SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q06215

- PPO_VICFA

UniProt

Q06215 - PPO_VICFA

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Polyphenol oxidase A1, chloroplastic

Gene
N/A
Organism
Vicia faba (Broad bean) (Faba vulgaris)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the oxidation of mono- and o-diphenols to o-diquinones.

Catalytic activityi

2 catechol + O2 = 2 1,2-benzoquinone + 2 H2O.

Cofactori

Binds 2 copper ions per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi181 – 1811Copper A By similarity
Metal bindingi202 – 2021Copper A By similarity
Metal bindingi211 – 2111Copper A By similarity
Metal bindingi333 – 3331Copper B By similarity
Metal bindingi337 – 3371Copper B By similarity
Metal bindingi367 – 3671Copper B By similarity

GO - Molecular functioni

  1. catechol oxidase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. pigment biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Copper, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Polyphenol oxidase A1, chloroplastic (EC:1.10.3.1)
Short name:
PPO
Alternative name(s):
Catechol oxidase
OrganismiVicia faba (Broad bean) (Faba vulgaris)
Taxonomic identifieri3906 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeFabeaeVicia

Subcellular locationi

GO - Cellular componenti

  1. chloroplast thylakoid lumen Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid, Thylakoid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 9292Chloroplast1 PublicationAdd
BLAST
Chaini93 – 606514Polyphenol oxidase A1, chloroplasticPRO_0000035919Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi103 ↔ 121 By similarity
Disulfide bondi120 ↔ 182 By similarity
Cross-linki185 ↔ 2022'-(S-cysteinyl)-histidine (Cys-His) By similarity

Keywords - PTMi

Disulfide bond, Thioether bond

Structurei

3D structure databases

ProteinModelPortaliQ06215.
SMRiQ06215. Positions 93-431.

Family & Domainsi

Sequence similaritiesi

Belongs to the tyrosinase family.

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di1.10.1280.10. 1 hit.
InterProiIPR016213. Polyphenol_oxidase.
IPR022740. Polyphenol_oxidase_C.
IPR022739. Polyphenol_oxidase_cen.
IPR002227. Tyrosinase_Cu-bd.
IPR008922. Unchr_di-copper_centre.
[Graphical view]
PfamiPF12142. PPO1_DWL. 1 hit.
PF12143. PPO1_KFDV. 1 hit.
PF00264. Tyrosinase. 1 hit.
[Graphical view]
PIRSFiPIRSF000290. PPO_plant. 1 hit.
PRINTSiPR00092. TYROSINASE.
SUPFAMiSSF48056. SSF48056. 1 hit.
PROSITEiPS00497. TYROSINASE_1. 1 hit.
PS00498. TYROSINASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q06215-1 [UniParc]FASTAAdd to Basket

« Hide

MTSISALSFI STINVSSNSK ISHSSVYPFL QKQHQSSKLR KPKRQVTCSS    50
NNNQNNPKEE QELSNIVGHR RNVLIGLGGI YGTLATNPSA LASPISPPDL 100
SKCVPPSDLP SGTTPPNINC CPPYSTKITD FKFPSNQPLR VRQAAHLVDN 150
EFLEKYKKAT ELMKALPSND PRNFTQQANI HCAYCDGAYS QIGFPDLKLQ 200
VHGSWLFFPF HRWYLYFYER ILGSLINDPT FALPFWNYDA PDGMQLPTIY 250
ADKASPLYDE LRNASHQPPT LIDLNFCDIG SDIDRNELIK TNLSIMYRQV 300
YSNGKTSRLF LGNPYRAGDA EPQGAGSIEN VPHAPVHTWT GDNTQTNIED 350
MGIFYSAARD PIFYSHHSNV DRLWYIWKTL GGKKHDFTDK DWLESGFLFY 400
DENKNLVRVN VKDSLDIDKL GYAYQDVPIP WEKAKPVPRR TKVQKLVEVE 450
VNDGNLRKSP TIFLVRQQSP RKYVTFPLVL NNKVSAIVKR PKKLRSKKEK 500
EEEEEVLVIE GIEFYMNIAI KFDVYINDED DKVGAGNTEF AGSFVNIPHS 550
AHGHKNKKII TSLRLGITDL LEDLHVEGDD NIVVTLVPKC GSGQVKINNV 600
EIVFED 606
Length:606
Mass (Da):68,541
Last modified:November 1, 1995 - v1
Checksum:iFC539F9F041B503C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z11702 mRNA. Translation: CAA77764.1.
PIRiS24758.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z11702 mRNA. Translation: CAA77764.1 .
PIRi S24758.

3D structure databases

ProteinModelPortali Q06215.
SMRi Q06215. Positions 93-431.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.10.1280.10. 1 hit.
InterProi IPR016213. Polyphenol_oxidase.
IPR022740. Polyphenol_oxidase_C.
IPR022739. Polyphenol_oxidase_cen.
IPR002227. Tyrosinase_Cu-bd.
IPR008922. Unchr_di-copper_centre.
[Graphical view ]
Pfami PF12142. PPO1_DWL. 1 hit.
PF12143. PPO1_KFDV. 1 hit.
PF00264. Tyrosinase. 1 hit.
[Graphical view ]
PIRSFi PIRSF000290. PPO_plant. 1 hit.
PRINTSi PR00092. TYROSINASE.
SUPFAMi SSF48056. SSF48056. 1 hit.
PROSITEi PS00497. TYROSINASE_1. 1 hit.
PS00498. TYROSINASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and characterization of cDNAs coding for Vicia faba polyphenol oxidase."
    Cary J.W., Lax A.R., Flurkey W.H.
    Plant Mol. Biol. 20:245-253(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Tissue: Leaf.
  2. "Polypeptide composition and amino-terminal sequence of broad bean polyphenol oxidase."
    Flurkey W.H.
    Plant Physiol. 91:481-483(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: PRELIMINARY PROTEIN SEQUENCE OF 93-119.
    Tissue: Leaf.

Entry informationi

Entry nameiPPO_VICFA
AccessioniPrimary (citable) accession number: Q06215
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: September 3, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi