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Q06215

- PPO_VICFA

UniProt

Q06215 - PPO_VICFA

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Protein

Polyphenol oxidase A1, chloroplastic

Gene
N/A
Organism
Vicia faba (Broad bean) (Faba vulgaris)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the oxidation of mono- and o-diphenols to o-diquinones.

Catalytic activityi

2 catechol + O2 = 2 1,2-benzoquinone + 2 H2O.

Cofactori

Binds 2 copper ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi181 – 1811Copper ABy similarity
Metal bindingi202 – 2021Copper ABy similarity
Metal bindingi211 – 2111Copper ABy similarity
Metal bindingi333 – 3331Copper BBy similarity
Metal bindingi337 – 3371Copper BBy similarity
Metal bindingi367 – 3671Copper BBy similarity

GO - Molecular functioni

  1. catechol oxidase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. pigment biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Copper, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Polyphenol oxidase A1, chloroplastic (EC:1.10.3.1)
Short name:
PPO
Alternative name(s):
Catechol oxidase
OrganismiVicia faba (Broad bean) (Faba vulgaris)
Taxonomic identifieri3906 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeFabeaeVicia

Subcellular locationi

GO - Cellular componenti

  1. chloroplast Source: UniProtKB-KW
  2. thylakoid Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid, Thylakoid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 9292ChloroplastAdd
BLAST
Chaini93 – 606514Polyphenol oxidase A1, chloroplasticPRO_0000035919Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi103 ↔ 121By similarity
Disulfide bondi120 ↔ 182By similarity
Cross-linki185 ↔ 2022'-(S-cysteinyl)-histidine (Cys-His)By similarity

Keywords - PTMi

Disulfide bond, Thioether bond

Structurei

3D structure databases

ProteinModelPortaliQ06215.
SMRiQ06215. Positions 93-431.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tyrosinase family.Curated

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di1.10.1280.10. 1 hit.
InterProiIPR016213. Polyphenol_oxidase.
IPR022740. Polyphenol_oxidase_C.
IPR022739. Polyphenol_oxidase_cen.
IPR002227. Tyrosinase_Cu-bd.
IPR008922. Unchr_di-copper_centre.
[Graphical view]
PfamiPF12142. PPO1_DWL. 1 hit.
PF12143. PPO1_KFDV. 1 hit.
PF00264. Tyrosinase. 1 hit.
[Graphical view]
PIRSFiPIRSF000290. PPO_plant. 1 hit.
PRINTSiPR00092. TYROSINASE.
SUPFAMiSSF48056. SSF48056. 1 hit.
PROSITEiPS00497. TYROSINASE_1. 1 hit.
PS00498. TYROSINASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q06215 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTSISALSFI STINVSSNSK ISHSSVYPFL QKQHQSSKLR KPKRQVTCSS
60 70 80 90 100
NNNQNNPKEE QELSNIVGHR RNVLIGLGGI YGTLATNPSA LASPISPPDL
110 120 130 140 150
SKCVPPSDLP SGTTPPNINC CPPYSTKITD FKFPSNQPLR VRQAAHLVDN
160 170 180 190 200
EFLEKYKKAT ELMKALPSND PRNFTQQANI HCAYCDGAYS QIGFPDLKLQ
210 220 230 240 250
VHGSWLFFPF HRWYLYFYER ILGSLINDPT FALPFWNYDA PDGMQLPTIY
260 270 280 290 300
ADKASPLYDE LRNASHQPPT LIDLNFCDIG SDIDRNELIK TNLSIMYRQV
310 320 330 340 350
YSNGKTSRLF LGNPYRAGDA EPQGAGSIEN VPHAPVHTWT GDNTQTNIED
360 370 380 390 400
MGIFYSAARD PIFYSHHSNV DRLWYIWKTL GGKKHDFTDK DWLESGFLFY
410 420 430 440 450
DENKNLVRVN VKDSLDIDKL GYAYQDVPIP WEKAKPVPRR TKVQKLVEVE
460 470 480 490 500
VNDGNLRKSP TIFLVRQQSP RKYVTFPLVL NNKVSAIVKR PKKLRSKKEK
510 520 530 540 550
EEEEEVLVIE GIEFYMNIAI KFDVYINDED DKVGAGNTEF AGSFVNIPHS
560 570 580 590 600
AHGHKNKKII TSLRLGITDL LEDLHVEGDD NIVVTLVPKC GSGQVKINNV

EIVFED
Length:606
Mass (Da):68,541
Last modified:November 1, 1995 - v1
Checksum:iFC539F9F041B503C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z11702 mRNA. Translation: CAA77764.1.
PIRiS24758.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z11702 mRNA. Translation: CAA77764.1 .
PIRi S24758.

3D structure databases

ProteinModelPortali Q06215.
SMRi Q06215. Positions 93-431.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.10.1280.10. 1 hit.
InterProi IPR016213. Polyphenol_oxidase.
IPR022740. Polyphenol_oxidase_C.
IPR022739. Polyphenol_oxidase_cen.
IPR002227. Tyrosinase_Cu-bd.
IPR008922. Unchr_di-copper_centre.
[Graphical view ]
Pfami PF12142. PPO1_DWL. 1 hit.
PF12143. PPO1_KFDV. 1 hit.
PF00264. Tyrosinase. 1 hit.
[Graphical view ]
PIRSFi PIRSF000290. PPO_plant. 1 hit.
PRINTSi PR00092. TYROSINASE.
SUPFAMi SSF48056. SSF48056. 1 hit.
PROSITEi PS00497. TYROSINASE_1. 1 hit.
PS00498. TYROSINASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and characterization of cDNAs coding for Vicia faba polyphenol oxidase."
    Cary J.W., Lax A.R., Flurkey W.H.
    Plant Mol. Biol. 20:245-253(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Tissue: Leaf.
  2. "Polypeptide composition and amino-terminal sequence of broad bean polyphenol oxidase."
    Flurkey W.H.
    Plant Physiol. 91:481-483(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: PRELIMINARY PROTEIN SEQUENCE OF 93-119.
    Tissue: Leaf.

Entry informationi

Entry nameiPPO_VICFA
AccessioniPrimary (citable) accession number: Q06215
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: October 29, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3