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Q06213

- MED10_YEAST

UniProt

Q06213 - MED10_YEAST

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Protein

Mediator of RNA polymerase II transcription subunit 10

Gene

NUT2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. The Mediator complex unfolds to an extended conformation and partially surrounds RNA polymerase II, specifically interacting with the unphosphorylated form of the C-terminal domain (CTD) of RNA polymerase II. The Mediator complex dissociates from the RNA polymerase II holoenzyme and stays at the promoter when transcriptional elongation begins.4 Publications

GO - Molecular functioni

  1. RNA polymerase II transcription cofactor activity Source: InterPro

GO - Biological processi

  1. negative regulation of transcription from RNA polymerase II promoter Source: SGD
  2. positive regulation of transcription from RNA polymerase II promoter Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-34296-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 10
Alternative name(s):
Mediator complex subunit 10
Negative regulator of URS2 protein 2
Gene namesi
Name:NUT2
Synonyms:MED10
Ordered Locus Names:YPR168W
ORF Names:P9325.2
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XVI

Organism-specific databases

CYGDiYPR168w.
SGDiS000006372. NUT2.

Subcellular locationi

Nucleus 1 Publication

GO - Cellular componenti

  1. core mediator complex Source: SGD
  2. mediator complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 157157Mediator of RNA polymerase II transcription subunit 10PRO_0000096357Add
BLAST

Proteomic databases

MaxQBiQ06213.
PaxDbiQ06213.

Expressioni

Gene expression databases

GenevestigatoriQ06213.

Interactioni

Subunit structurei

Component of the Mediator complex, which is composed of at least 21 subunits that form three structurally distinct submodules. The Mediator head module contains MED6, MED8, MED11, SRB4/MED17, SRB5/MED18, ROX3/MED19, SRB2/MED20 and SRB6/MED22, the middle module contains MED1, MED4, NUT1/MED5, MED7, CSE2/MED9, NUT2/MED10, SRB7/MED21 and SOH1/MED31, and the tail module contains MED2, PGD1/MED3, RGR1/MED14, GAL11/MED15 and SIN4/MED16. The head and the middle modules interact directly with RNA polymerase II, whereas the elongated tail module interacts with gene-specific regulatory proteins. NUT2/MED10 interacts directly with SRB7/MED21.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
LSM1P470171EBI-12414,EBI-174
SOH1P386331EBI-12414,EBI-17658

Protein-protein interaction databases

BioGridi36341. 44 interactions.
DIPiDIP-3922N.
IntActiQ06213. 10 interactions.
MINTiMINT-503239.
STRINGi4932.YPR168W.

Structurei

3D structure databases

ProteinModelPortaliQ06213.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Mediator complex subunit 10 family.Curated

Phylogenomic databases

eggNOGiNOG245504.
HOGENOMiHOG000001009.
InParanoidiQ06213.
KOiK15151.
OMAiNAKSNAR.
OrthoDBiEOG7DZ8XP.

Family and domain databases

InterProiIPR019145. Mediator_Med10.
[Graphical view]
PfamiPF09748. Med10. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q06213-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNGNSTNNEQ LQQELATTQD QVASIIESFV ELGVSIYDFP GTPEATKGMI
60 70 80 90 100
TNLQRNVDRL YKLNVRSNDP QSSLSKVDIP LEVVQYIEDG RNPDIYTREF
110 120 130 140 150
VEAIRRSNQY QRGKMHGLKQ LRDSLADKIV DEFPELKEPV EDIIKRTSPI

DNVSNTH
Length:157
Mass (Da):17,908
Last modified:November 1, 1996 - v1
Checksum:i0E77A43A5222E862
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U25840 Genomic DNA. Translation: AAB68155.1.
BK006949 Genomic DNA. Translation: DAA11585.1.
PIRiS59827.
RefSeqiNP_015494.1. NM_001184265.1.

Genome annotation databases

EnsemblFungiiYPR168W; YPR168W; YPR168W.
GeneIDi856297.
KEGGisce:YPR168W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U25840 Genomic DNA. Translation: AAB68155.1 .
BK006949 Genomic DNA. Translation: DAA11585.1 .
PIRi S59827.
RefSeqi NP_015494.1. NM_001184265.1.

3D structure databases

ProteinModelPortali Q06213.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36341. 44 interactions.
DIPi DIP-3922N.
IntActi Q06213. 10 interactions.
MINTi MINT-503239.
STRINGi 4932.YPR168W.

Proteomic databases

MaxQBi Q06213.
PaxDbi Q06213.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YPR168W ; YPR168W ; YPR168W .
GeneIDi 856297.
KEGGi sce:YPR168W.

Organism-specific databases

CYGDi YPR168w.
SGDi S000006372. NUT2.

Phylogenomic databases

eggNOGi NOG245504.
HOGENOMi HOG000001009.
InParanoidi Q06213.
KOi K15151.
OMAi NAKSNAR.
OrthoDBi EOG7DZ8XP.

Enzyme and pathway databases

BioCyci YEAST:G3O-34296-MONOMER.

Miscellaneous databases

NextBioi 981650.
PROi Q06213.

Gene expression databases

Genevestigatori Q06213.

Family and domain databases

InterProi IPR019145. Mediator_Med10.
[Graphical view ]
Pfami PF09748. Med10. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Nuclear proteins Nut1p and Nut2p cooperate to negatively regulate a Swi4p-dependent lacZ reporter gene in Saccharomyces cerevisiae."
    Tabtiang R.K., Herskowitz I.
    Mol. Cell. Biol. 18:4707-4718(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. "Identification of new mediator subunits in the RNA polymerase II holoenzyme from Saccharomyces cerevisiae."
    Gustafsson C.M., Myers L.C., Beve J., Spaahr H., Lui M., Erdjument-Bromage H., Tempst P., Kornberg R.D.
    J. Biol. Chem. 273:30851-30854(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: COMPONENT OF MEDIATOR COMPLEX.
  5. "The structural and functional organization of the yeast mediator complex."
    Kang J.S., Kim S.H., Hwang M.S., Han S.J., Lee Y.C., Kim Y.-J.
    J. Biol. Chem. 276:42003-42010(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SRB7, FUNCTION OF THE MEDIATOR COMPLEX, INTERACTION OF THE MEDIATOR COMPLEX WITH RNA POLYMERASE II.
  6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. Cited for: NOMENCLATURE.
  9. Cited for: TOPOLOGY OF THE MEDIATOR COMPLEX.
  10. "A conserved mediator hinge revealed in the structure of the MED7-MED21 (Med7-Srb7) heterodimer."
    Baumli S., Hoeppner S., Cramer P.
    J. Biol. Chem. 280:18171-18178(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SRB7.
  11. "Preponderance of free mediator in the yeast Saccharomyces cerevisiae."
    Takagi Y., Chadick J.Z., Davis J.A., Asturias F.J.
    J. Biol. Chem. 280:31200-31207(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION OF THE MEDIATOR COMPLEX.
  12. "Mediator and TFIIH govern carboxyl-terminal domain-dependent transcription in yeast extracts."
    Nair D., Kim Y., Myers L.C.
    J. Biol. Chem. 280:33739-33748(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE MEDIATOR COMPLEX.
  13. "Mediator as a general transcription factor."
    Takagi Y., Kornberg R.D.
    J. Biol. Chem. 281:80-89(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE MEDIATOR COMPLEX.
  14. "Med19(Rox3) regulates intermodule interactions in the Saccharomyces cerevisiae mediator complex."
    Baidoobonso S.M., Guidi B.W., Myers L.C.
    J. Biol. Chem. 282:5551-5559(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION OF THE MEDIATOR COMPLEX, INTERACTION OF THE MEDIATOR COMPLEX WITH RNA POLYMERASE II.
  15. "Structure of the yeast RNA polymerase II holoenzyme: mediator conformation and polymerase interaction."
    Davis J.A., Takagi Y., Kornberg R.D., Asturias F.J.
    Mol. Cell 10:409-415(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: ELECTRON MICROSCOPY OF MEDIATOR COMPLEX IN COMPLEX WITH RNA POLYMERASE II.

Entry informationi

Entry nameiMED10_YEAST
AccessioniPrimary (citable) accession number: Q06213
Secondary accession number(s): D6W4G9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1472 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

External Data

Dasty 3