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Q06213

- MED10_YEAST

UniProt

Q06213 - MED10_YEAST

Protein

Mediator of RNA polymerase II transcription subunit 10

Gene

NUT2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. The Mediator complex unfolds to an extended conformation and partially surrounds RNA polymerase II, specifically interacting with the unphosphorylated form of the C-terminal domain (CTD) of RNA polymerase II. The Mediator complex dissociates from the RNA polymerase II holoenzyme and stays at the promoter when transcriptional elongation begins.4 Publications

    GO - Molecular functioni

    1. RNA polymerase II transcription cofactor activity Source: InterPro

    GO - Biological processi

    1. negative regulation of transcription from RNA polymerase II promoter Source: SGD
    2. positive regulation of transcription from RNA polymerase II promoter Source: SGD

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation

    Enzyme and pathway databases

    BioCyciYEAST:G3O-34296-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mediator of RNA polymerase II transcription subunit 10
    Alternative name(s):
    Mediator complex subunit 10
    Negative regulator of URS2 protein 2
    Gene namesi
    Name:NUT2
    Synonyms:MED10
    Ordered Locus Names:YPR168W
    ORF Names:P9325.2
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XVI

    Organism-specific databases

    CYGDiYPR168w.
    SGDiS000006372. NUT2.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. core mediator complex Source: SGD
    2. mediator complex Source: InterPro

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 157157Mediator of RNA polymerase II transcription subunit 10PRO_0000096357Add
    BLAST

    Proteomic databases

    MaxQBiQ06213.
    PaxDbiQ06213.

    Expressioni

    Gene expression databases

    GenevestigatoriQ06213.

    Interactioni

    Subunit structurei

    Component of the Mediator complex, which is composed of at least 21 subunits that form three structurally distinct submodules. The Mediator head module contains MED6, MED8, MED11, SRB4/MED17, SRB5/MED18, ROX3/MED19, SRB2/MED20 and SRB6/MED22, the middle module contains MED1, MED4, NUT1/MED5, MED7, CSE2/MED9, NUT2/MED10, SRB7/MED21 and SOH1/MED31, and the tail module contains MED2, PGD1/MED3, RGR1/MED14, GAL11/MED15 and SIN4/MED16. The head and the middle modules interact directly with RNA polymerase II, whereas the elongated tail module interacts with gene-specific regulatory proteins. NUT2/MED10 interacts directly with SRB7/MED21.3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    LSM1P470171EBI-12414,EBI-174
    SOH1P386331EBI-12414,EBI-17658

    Protein-protein interaction databases

    BioGridi36341. 44 interactions.
    DIPiDIP-3922N.
    IntActiQ06213. 10 interactions.
    MINTiMINT-503239.
    STRINGi4932.YPR168W.

    Structurei

    3D structure databases

    ProteinModelPortaliQ06213.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the Mediator complex subunit 10 family.Curated

    Phylogenomic databases

    eggNOGiNOG245504.
    HOGENOMiHOG000001009.
    KOiK15151.
    OMAiNAKSNAR.
    OrthoDBiEOG7DZ8XP.

    Family and domain databases

    InterProiIPR019145. Mediator_Med10.
    [Graphical view]
    PfamiPF09748. Med10. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q06213-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNGNSTNNEQ LQQELATTQD QVASIIESFV ELGVSIYDFP GTPEATKGMI    50
    TNLQRNVDRL YKLNVRSNDP QSSLSKVDIP LEVVQYIEDG RNPDIYTREF 100
    VEAIRRSNQY QRGKMHGLKQ LRDSLADKIV DEFPELKEPV EDIIKRTSPI 150
    DNVSNTH 157
    Length:157
    Mass (Da):17,908
    Last modified:November 1, 1996 - v1
    Checksum:i0E77A43A5222E862
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U25840 Genomic DNA. Translation: AAB68155.1.
    BK006949 Genomic DNA. Translation: DAA11585.1.
    PIRiS59827.
    RefSeqiNP_015494.1. NM_001184265.1.

    Genome annotation databases

    EnsemblFungiiYPR168W; YPR168W; YPR168W.
    GeneIDi856297.
    KEGGisce:YPR168W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U25840 Genomic DNA. Translation: AAB68155.1 .
    BK006949 Genomic DNA. Translation: DAA11585.1 .
    PIRi S59827.
    RefSeqi NP_015494.1. NM_001184265.1.

    3D structure databases

    ProteinModelPortali Q06213.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36341. 44 interactions.
    DIPi DIP-3922N.
    IntActi Q06213. 10 interactions.
    MINTi MINT-503239.
    STRINGi 4932.YPR168W.

    Proteomic databases

    MaxQBi Q06213.
    PaxDbi Q06213.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YPR168W ; YPR168W ; YPR168W .
    GeneIDi 856297.
    KEGGi sce:YPR168W.

    Organism-specific databases

    CYGDi YPR168w.
    SGDi S000006372. NUT2.

    Phylogenomic databases

    eggNOGi NOG245504.
    HOGENOMi HOG000001009.
    KOi K15151.
    OMAi NAKSNAR.
    OrthoDBi EOG7DZ8XP.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-34296-MONOMER.

    Miscellaneous databases

    NextBioi 981650.
    PROi Q06213.

    Gene expression databases

    Genevestigatori Q06213.

    Family and domain databases

    InterProi IPR019145. Mediator_Med10.
    [Graphical view ]
    Pfami PF09748. Med10. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
      Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
      , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
      Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "Nuclear proteins Nut1p and Nut2p cooperate to negatively regulate a Swi4p-dependent lacZ reporter gene in Saccharomyces cerevisiae."
      Tabtiang R.K., Herskowitz I.
      Mol. Cell. Biol. 18:4707-4718(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    4. "Identification of new mediator subunits in the RNA polymerase II holoenzyme from Saccharomyces cerevisiae."
      Gustafsson C.M., Myers L.C., Beve J., Spaahr H., Lui M., Erdjument-Bromage H., Tempst P., Kornberg R.D.
      J. Biol. Chem. 273:30851-30854(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: COMPONENT OF MEDIATOR COMPLEX.
    5. "The structural and functional organization of the yeast mediator complex."
      Kang J.S., Kim S.H., Hwang M.S., Han S.J., Lee Y.C., Kim Y.-J.
      J. Biol. Chem. 276:42003-42010(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SRB7, FUNCTION OF THE MEDIATOR COMPLEX, INTERACTION OF THE MEDIATOR COMPLEX WITH RNA POLYMERASE II.
    6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. Cited for: NOMENCLATURE.
    9. Cited for: TOPOLOGY OF THE MEDIATOR COMPLEX.
    10. "A conserved mediator hinge revealed in the structure of the MED7-MED21 (Med7-Srb7) heterodimer."
      Baumli S., Hoeppner S., Cramer P.
      J. Biol. Chem. 280:18171-18178(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SRB7.
    11. "Preponderance of free mediator in the yeast Saccharomyces cerevisiae."
      Takagi Y., Chadick J.Z., Davis J.A., Asturias F.J.
      J. Biol. Chem. 280:31200-31207(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION OF THE MEDIATOR COMPLEX.
    12. "Mediator and TFIIH govern carboxyl-terminal domain-dependent transcription in yeast extracts."
      Nair D., Kim Y., Myers L.C.
      J. Biol. Chem. 280:33739-33748(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF THE MEDIATOR COMPLEX.
    13. "Mediator as a general transcription factor."
      Takagi Y., Kornberg R.D.
      J. Biol. Chem. 281:80-89(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF THE MEDIATOR COMPLEX.
    14. "Med19(Rox3) regulates intermodule interactions in the Saccharomyces cerevisiae mediator complex."
      Baidoobonso S.M., Guidi B.W., Myers L.C.
      J. Biol. Chem. 282:5551-5559(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION OF THE MEDIATOR COMPLEX, INTERACTION OF THE MEDIATOR COMPLEX WITH RNA POLYMERASE II.
    15. "Structure of the yeast RNA polymerase II holoenzyme: mediator conformation and polymerase interaction."
      Davis J.A., Takagi Y., Kornberg R.D., Asturias F.J.
      Mol. Cell 10:409-415(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: ELECTRON MICROSCOPY OF MEDIATOR COMPLEX IN COMPLEX WITH RNA POLYMERASE II.

    Entry informationi

    Entry nameiMED10_YEAST
    AccessioniPrimary (citable) accession number: Q06213
    Secondary accession number(s): D6W4G9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 105 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1472 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XVI
      Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

    External Data

    Dasty 3