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Q06213 (MED10_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mediator of RNA polymerase II transcription subunit 10
Alternative name(s):
Mediator complex subunit 10
Negative regulator of URS2 protein 2
Gene names
Name:NUT2
Synonyms:MED10
Ordered Locus Names:YPR168W
ORF Names:P9325.2
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length157 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. The Mediator complex unfolds to an extended conformation and partially surrounds RNA polymerase II, specifically interacting with the unphosphorylated form of the C-terminal domain (CTD) of RNA polymerase II. The Mediator complex dissociates from the RNA polymerase II holoenzyme and stays at the promoter when transcriptional elongation begins. Ref.3 Ref.5 Ref.12 Ref.13

Subunit structure

Component of the Mediator complex, which is composed of at least 21 subunits that form three structurally distinct submodules. The Mediator head module contains MED6, MED8, MED11, SRB4/MED17, SRB5/MED18, ROX3/MED19, SRB2/MED20 and SRB6/MED22, the middle module contains MED1, MED4, NUT1/MED5, MED7, CSE2/MED9, NUT2/MED10, SRB7/MED21 and SOH1/MED31, and the tail module contains MED2, PGD1/MED3, RGR1/MED14, GAL11/MED15 and SIN4/MED16. The head and the middle modules interact directly with RNA polymerase II, whereas the elongated tail module interacts with gene-specific regulatory proteins. NUT2/MED10 interacts directly with SRB7/MED21. Ref.5 Ref.10 Ref.14

Subcellular location

Nucleus Ref.6.

Miscellaneous

Present with 1472 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the Mediator complex subunit 10 family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

LSM1P470171EBI-12414,EBI-174
SOH1P386331EBI-12414,EBI-17658

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 157157Mediator of RNA polymerase II transcription subunit 10
PRO_0000096357

Sequences

Sequence LengthMass (Da)Tools
Q06213 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 0E77A43A5222E862

FASTA15717,908
        10         20         30         40         50         60 
MNGNSTNNEQ LQQELATTQD QVASIIESFV ELGVSIYDFP GTPEATKGMI TNLQRNVDRL 

        70         80         90        100        110        120 
YKLNVRSNDP QSSLSKVDIP LEVVQYIEDG RNPDIYTREF VEAIRRSNQY QRGKMHGLKQ 

       130        140        150 
LRDSLADKIV DEFPELKEPV EDIIKRTSPI DNVSNTH 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. expand/collapse author list , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Nuclear proteins Nut1p and Nut2p cooperate to negatively regulate a Swi4p-dependent lacZ reporter gene in Saccharomyces cerevisiae."
Tabtiang R.K., Herskowitz I.
Mol. Cell. Biol. 18:4707-4718(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[4]"Identification of new mediator subunits in the RNA polymerase II holoenzyme from Saccharomyces cerevisiae."
Gustafsson C.M., Myers L.C., Beve J., Spaahr H., Lui M., Erdjument-Bromage H., Tempst P., Kornberg R.D.
J. Biol. Chem. 273:30851-30854(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: COMPONENT OF MEDIATOR COMPLEX.
[5]"The structural and functional organization of the yeast mediator complex."
Kang J.S., Kim S.H., Hwang M.S., Han S.J., Lee Y.C., Kim Y.-J.
J. Biol. Chem. 276:42003-42010(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SRB7, FUNCTION OF THE MEDIATOR COMPLEX, INTERACTION OF THE MEDIATOR COMPLEX WITH RNA POLYMERASE II.
[6]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]"A unified nomenclature for protein subunits of mediator complexes linking transcriptional regulators to RNA polymerase II."
Bourbon H.-M., Aguilera A., Ansari A.Z., Asturias F.J., Berk A.J., Bjoerklund S., Blackwell T.K., Borggrefe T., Carey M., Carlson M., Conaway J.W., Conaway R.C., Emmons S.W., Fondell J.D., Freedman L.P., Fukasawa T., Gustafsson C.M., Han M. expand/collapse author list , He X., Herman P.K., Hinnebusch A.G., Holmberg S., Holstege F.C.P., Jaehning J.A., Kim Y.-J., Kuras L., Leutz A., Lis J.T., Meisterernest M., Naeaer A.M., Nasmyth K., Parvin J.D., Ptashne M., Reinberg D., Ronne H., Sadowski I., Sakurai H., Sipiczki M., Sternberg P.W., Stillman D.J., Strich R., Struhl K., Svejstrup J.Q., Tuck S., Winston F., Roeder R.G., Kornberg R.D.
Mol. Cell 14:553-557(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NOMENCLATURE.
[9]"A high resolution protein interaction map of the yeast Mediator complex."
Guglielmi B., van Berkum N.L., Klapholz B., Bijma T., Boube M., Boschiero C., Bourbon H.-M., Holstege F.C.P., Werner M.
Nucleic Acids Res. 32:5379-5391(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY OF THE MEDIATOR COMPLEX.
[10]"A conserved mediator hinge revealed in the structure of the MED7-MED21 (Med7-Srb7) heterodimer."
Baumli S., Hoeppner S., Cramer P.
J. Biol. Chem. 280:18171-18178(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SRB7.
[11]"Preponderance of free mediator in the yeast Saccharomyces cerevisiae."
Takagi Y., Chadick J.Z., Davis J.A., Asturias F.J.
J. Biol. Chem. 280:31200-31207(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION OF THE MEDIATOR COMPLEX.
[12]"Mediator and TFIIH govern carboxyl-terminal domain-dependent transcription in yeast extracts."
Nair D., Kim Y., Myers L.C.
J. Biol. Chem. 280:33739-33748(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION OF THE MEDIATOR COMPLEX.
[13]"Mediator as a general transcription factor."
Takagi Y., Kornberg R.D.
J. Biol. Chem. 281:80-89(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION OF THE MEDIATOR COMPLEX.
[14]"Med19(Rox3) regulates intermodule interactions in the Saccharomyces cerevisiae mediator complex."
Baidoobonso S.M., Guidi B.W., Myers L.C.
J. Biol. Chem. 282:5551-5559(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION OF THE MEDIATOR COMPLEX, INTERACTION OF THE MEDIATOR COMPLEX WITH RNA POLYMERASE II.
[15]"Structure of the yeast RNA polymerase II holoenzyme: mediator conformation and polymerase interaction."
Davis J.A., Takagi Y., Kornberg R.D., Asturias F.J.
Mol. Cell 10:409-415(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: ELECTRON MICROSCOPY OF MEDIATOR COMPLEX IN COMPLEX WITH RNA POLYMERASE II.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U25840 Genomic DNA. Translation: AAB68155.1.
BK006949 Genomic DNA. Translation: DAA11585.1.
PIRS59827.
RefSeqNP_015494.1. NM_001184265.1.

3D structure databases

ProteinModelPortalQ06213.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36341. 44 interactions.
DIPDIP-3922N.
IntActQ06213. 10 interactions.
MINTMINT-503239.
STRING4932.YPR168W.

Proteomic databases

MaxQBQ06213.
PaxDbQ06213.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYPR168W; YPR168W; YPR168W.
GeneID856297.
KEGGsce:YPR168W.

Organism-specific databases

CYGDYPR168w.
SGDS000006372. NUT2.

Phylogenomic databases

eggNOGNOG245504.
HOGENOMHOG000001009.
KOK15151.
OMANAKSNAR.
OrthoDBEOG7DZ8XP.

Enzyme and pathway databases

BioCycYEAST:G3O-34296-MONOMER.

Gene expression databases

GenevestigatorQ06213.

Family and domain databases

InterProIPR019145. Mediator_Med10.
[Graphical view]
PfamPF09748. Med10. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio981650.
PROQ06213.

Entry information

Entry nameMED10_YEAST
AccessionPrimary (citable) accession number: Q06213
Secondary accession number(s): D6W4G9
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: May 14, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XVI

Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families