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Protein

Mediator of RNA polymerase II transcription subunit 10

Gene

NUT2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. The Mediator complex unfolds to an extended conformation and partially surrounds RNA polymerase II, specifically interacting with the unphosphorylated form of the C-terminal domain (CTD) of RNA polymerase II. The Mediator complex dissociates from the RNA polymerase II holoenzyme and stays at the promoter when transcriptional elongation begins.4 Publications

Miscellaneous

Present with 1472 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

GO - Biological processi

  • negative regulation of transcription by RNA polymerase II Source: SGD
  • positive regulation of transcription by RNA polymerase II Source: SGD
  • RNA polymerase II transcriptional preinitiation complex assembly Source: SGD

Keywordsi

Molecular functionActivator
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-34296-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 10
Alternative name(s):
Mediator complex subunit 10
Negative regulator of URS2 protein 2
Gene namesi
Name:NUT2
Synonyms:MED10
Ordered Locus Names:YPR168W
ORF Names:P9325.2
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPR168W
SGDiS000006372 NUT2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000963571 – 157Mediator of RNA polymerase II transcription subunit 10Add BLAST157

Proteomic databases

MaxQBiQ06213
PaxDbiQ06213
PRIDEiQ06213

Interactioni

Subunit structurei

Component of the Mediator complex, which is composed of at least 21 subunits that form three structurally distinct submodules. The Mediator head module contains MED6, MED8, MED11, SRB4/MED17, SRB5/MED18, ROX3/MED19, SRB2/MED20 and SRB6/MED22, the middle module contains MED1, MED4, NUT1/MED5, MED7, CSE2/MED9, NUT2/MED10, SRB7/MED21 and SOH1/MED31, and the tail module contains MED2, PGD1/MED3, RGR1/MED14, GAL11/MED15 and SIN4/MED16. The head and the middle modules interact directly with RNA polymerase II, whereas the elongated tail module interacts with gene-specific regulatory proteins. NUT2/MED10 interacts directly with SRB7/MED21.3 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi36341, 325 interactors
ComplexPortaliCPX-3226 Core mediator complex
DIPiDIP-3922N
IntActiQ06213, 25 interactors
MINTiQ06213
STRINGi4932.YPR168W

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5OQMelectron microscopy5.80k1-157[»]
ProteinModelPortaliQ06213
SMRiQ06213
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Mediator complex subunit 10 family.Curated

Phylogenomic databases

HOGENOMiHOG000001009
InParanoidiQ06213
KOiK15151
OMAiRNEQVKG
OrthoDBiEOG092C4ZNB

Family and domain databases

InterProiView protein in InterPro
IPR019145 Mediator_Med10
PfamiView protein in Pfam
PF09748 Med10, 1 hit

Sequencei

Sequence statusi: Complete.

Q06213-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNGNSTNNEQ LQQELATTQD QVASIIESFV ELGVSIYDFP GTPEATKGMI
60 70 80 90 100
TNLQRNVDRL YKLNVRSNDP QSSLSKVDIP LEVVQYIEDG RNPDIYTREF
110 120 130 140 150
VEAIRRSNQY QRGKMHGLKQ LRDSLADKIV DEFPELKEPV EDIIKRTSPI

DNVSNTH
Length:157
Mass (Da):17,908
Last modified:November 1, 1996 - v1
Checksum:i0E77A43A5222E862
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U25840 Genomic DNA Translation: AAB68155.1
BK006949 Genomic DNA Translation: DAA11585.1
PIRiS59827
RefSeqiNP_015494.1, NM_001184265.1

Genome annotation databases

EnsemblFungiiYPR168W; YPR168W; YPR168W
GeneIDi856297
KEGGisce:YPR168W

Similar proteinsi

Entry informationi

Entry nameiMED10_YEAST
AccessioniPrimary (citable) accession number: Q06213
Secondary accession number(s): D6W4G9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: June 20, 2018
This is version 133 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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