Reviewed, UniProtKB/Swiss-Prot Q06209 (CHI4_BRANA)

Names and origin

Protein names	Recommended name:     Basic endochitinase CHB4     EC=3.2.1.14
Organism	Brassica napus (Rape)
Taxonomic identifier	3708 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Brassica

Protein attributes

Sequence length	268 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Defense against chitin containing fungal pathogens.
Catalytic activity	Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.
Subcellular location	Secreted › extracellular space.
Induction	By infection with the fungal pathogen Phoma lingam.
Sequence similarities	Belongs to the glycosyl hydrolase 19 family. Chitinase class I subfamily. Contains 1 chitin-binding type-1 domain.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Chitin degradation Plant defense Polysaccharide degradation
Cellular component	Secreted
Domain	Signal
Ligand	Chitin-binding
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	cell wall macromolecule catabolic process Inferred from electronic annotation. Source: InterPro chitin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW defense response Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	chitin binding Inferred from electronic annotation. Source: UniProtKB-KW chitinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Chain	25 – 268	244	Basic endochitinase CHB4		PRO_0000005291
Regions
	Domain	25 – 59	35	Chitin-binding type-1
	Region	71 – 268	198	Catalytic
	Compositional bias	60 – 70	11	Gly/Pro-rich (hinge region)
Amino acid modifications
	Glycosylation	265	1	N-linked (GlcNAc...) Potential
	Disulfide bond	27 ↔ 35		By similarity
	Disulfide bond	29 ↔ 41		By similarity
	Disulfide bond	34 ↔ 48		By similarity
	Disulfide bond	52 ↔ 57		By similarity
	Disulfide bond	92 ↔ 137		By similarity
	Disulfide bond	150 ↔ 159		By similarity
	Disulfide bond	236 ↔ 268		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q06209-1 [UniParc]. Last modified October 1, 1994. Version 2. Checksum: 066BAC7BC699A0D6	FASTA	268	28,733
	10 20 30 40 50 60 MALTKLSLVL FLCFLGLYSE TVKSQNCGCA PNLCCSQFGY CGSTDAYCGT GCRSGPCRSP 70 80 90 100 110 120 GGTPSPPGGG SVGSIVTQAF FNGIINQAGG GCAGKNFYTR DSFINAANTF PNFANSVTRR 130 140 150 160 170 180 EIATMFAHFT HETGHFCYIE EINGASRDYC DENNRQYPCA PGKGYFGRGP IQLSWNYNYG 190 200 210 220 230 240 ACGQSLNLNL LGQPELVSSN PTVAFRTGLW FWMNSVRPVL NQGFGATIRA INGMECNGGN 250 260 SGAVNARIRY YRDYCGQLGV DPGPNLSC

References

[1]	"Cloning and characterization of a pathogen-induced chitinase in Brassica napus." Rasmussen U., Bojsen K., Collinge D.B. Plant Mol. Biol. 20:277-287(1992) [PubMed: 1391771] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Bienvenu.
[2]	"Induction and purification of chitinase in Brassica napus L. spp. oleifera infected with Phoma lingam." Rasmussen U., Giese H., Mikkelsen J.D. Planta 187:328-334(1992) Cited for: PROTEIN SEQUENCE OF 101-115 AND 253-267. Strain: cv. Bienvenu. Tissue: Cotyledon.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X61488 mRNA. Translation: CAA43708.1.
PIR	S25311.
3D structure databases
SMR	Q06209. Positions 29-55, 69-268.
ModBase	Search...
Protein family/group databases
CAZy	CBM18. Carbohydrate-Binding Module Family 18. GH19. Glycoside Hydrolase Family 19.
Enzyme and pathway databases
BRENDA	3.2.1.14. 393.
Family and domain databases
InterPro	IPR018371. Chitin-binding_1_CS. IPR001002. Chitin_bd_1. IPR016283. Glyco_hydro_19. IPR000726. Glyco_hydro_19_cat. [Graphical view]
PANTHER	PTHR22595. Glyco_hydro_19_cat. 1 hit.
Pfam	PF00187. Chitin_bind_1. 1 hit. PF00182. Glyco_hydro_19. 1 hit. [Graphical view]
PIRSF	PIRSF001060. Endochitinase. 1 hit.
PRINTS	PR00451. CHITINBINDNG.
ProDom	PD000609. Chitin_bd_1. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00270. ChtBD1. 1 hit. [Graphical view]
PROSITE	PS00026. CHIT_BIND_I_1. 1 hit. PS50941. CHIT_BIND_I_2. 1 hit. PS00773. CHITINASE_19_1. 1 hit. PS00774. CHITINASE_19_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHI4_BRANA

Accession

Primary (citable) accession number: Q06209

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	October 1, 1994
Last modified:	February 9, 2010
This is version 80 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)