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Q06203

- PUR1_HUMAN

UniProt

Q06203 - PUR1_HUMAN

Protein

Amidophosphoribosyltransferase

Gene

PPAT

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 147 (01 Oct 2014)
      Sequence version 1 (01 Jun 1994)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.

    Cofactori

    Binds 1 magnesium ion per subunit.By similarity
    Binds 1 4Fe-4S cluster per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei12 – 121For GATase activityBy similarity
    Metal bindingi280 – 2801Iron-sulfur (4Fe-4S)By similarity
    Metal bindingi327 – 3271MagnesiumBy similarity
    Metal bindingi389 – 3891MagnesiumBy similarity
    Metal bindingi390 – 3901MagnesiumBy similarity
    Metal bindingi426 – 4261Iron-sulfur (4Fe-4S)By similarity
    Metal bindingi503 – 5031Iron-sulfur (4Fe-4S)By similarity
    Metal bindingi506 – 5061Iron-sulfur (4Fe-4S)By similarity

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    2. amidophosphoribosyltransferase activity Source: ProtInc
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
    2. cellular response to drug Source: Ensembl
    3. cellular response to insulin stimulus Source: Ensembl
    4. G1/S transition of mitotic cell cycle Source: Ensembl
    5. glutamine catabolic process Source: Ensembl
    6. kidney development Source: Ensembl
    7. lactation Source: Ensembl
    8. maternal process involved in female pregnancy Source: Ensembl
    9. nucleobase-containing small molecule metabolic process Source: Reactome
    10. nucleoside metabolic process Source: InterPro
    11. organ regeneration Source: Ensembl
    12. protein homotetramerization Source: Ensembl
    13. purine nucleobase biosynthetic process Source: InterPro
    14. purine nucleobase metabolic process Source: Reactome
    15. purine nucleotide biosynthetic process Source: ProtInc
    16. purine ribonucleoside monophosphate biosynthetic process Source: Reactome
    17. small molecule metabolic process Source: Reactome

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Purine biosynthesis

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:HS05157-MONOMER.
    ReactomeiREACT_1776. Purine ribonucleoside monophosphate biosynthesis.
    UniPathwayiUPA00074; UER00124.

    Protein family/group databases

    MEROPSiC44.001.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Amidophosphoribosyltransferase (EC:2.4.2.14)
    Short name:
    ATase
    Alternative name(s):
    Glutamine phosphoribosylpyrophosphate amidotransferase
    Short name:
    GPAT
    Gene namesi
    Name:PPAT
    Synonyms:GPAT
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 4

    Organism-specific databases

    HGNCiHGNC:9238. PPAT.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: Reactome

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA33559.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Propeptidei1 – 1111CuratedPRO_0000029283Add
    BLAST
    Chaini12 – 517506AmidophosphoribosyltransferasePRO_0000029284Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiQ06203.
    PaxDbiQ06203.
    PeptideAtlasiQ06203.
    PRIDEiQ06203.

    PTM databases

    PhosphoSiteiQ06203.

    Expressioni

    Tissue specificityi

    Ubiquitously expressed.

    Gene expression databases

    ArrayExpressiQ06203.
    BgeeiQ06203.
    CleanExiHS_PPAT.
    GenevestigatoriQ06203.

    Organism-specific databases

    HPAiHPA036091.
    HPA036092.

    Interactioni

    Subunit structurei

    Homotetramer.

    Protein-protein interaction databases

    BioGridi111467. 19 interactions.
    IntActiQ06203. 2 interactions.
    STRINGi9606.ENSP00000264220.

    Structurei

    3D structure databases

    ProteinModelPortaliQ06203.
    SMRiQ06203. Positions 12-515.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini12 – 261250Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.Curated
    Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Glutamine amidotransferase

    Phylogenomic databases

    eggNOGiCOG0034.
    HOGENOMiHOG000033688.
    HOVERGENiHBG002589.
    InParanoidiQ06203.
    KOiK00764.
    OMAiIPVGDMN.
    OrthoDBiEOG789C9W.
    PhylomeDBiQ06203.
    TreeFamiTF106370.

    Family and domain databases

    Gene3Di3.40.50.2020. 1 hit.
    3.60.20.10. 1 hit.
    InterProiIPR005854. Amd_phspho_trans.
    IPR017932. GATase_2_dom.
    IPR000583. GATase_dom.
    IPR029055. Ntn_hydrolases_N.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    [Graphical view]
    PfamiPF00310. GATase_2. 2 hits.
    PF00156. Pribosyltran. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
    SUPFAMiSSF53271. SSF53271. 1 hit.
    SSF56235. SSF56235. 1 hit.
    TIGRFAMsiTIGR01134. purF. 1 hit.
    PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
    PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q06203-1 [UniParc]FASTAAdd to Basket

    « Hide

    MELEELGIRE ECGVFGCIAS GEWPTQLDVP HVITLGLVGL QHRGQESAGI    50
    VTSDGSSVPT FKSHKGMGLV NHVFTEDNLK KLYVSNLGIG HTRYATTGKC 100
    ELENCQPFVV ETLHGKIAVA HNGELVNAAR LRKKLLRHGI GLSTSSDSEM 150
    ITQLLAYTPP QEQDDTPDWV ARIKNLMKEA PTAYSLLIMH RDVIYAVRDP 200
    YGNRPLCIGR LIPVSDINDK EKKTSETEGW VVSSESCSFL SIGARYYREV 250
    LPGEIVEISR HNVQTLDIIS RSEGNPVAFC IFEYVYFARP DSMFEDQMVY 300
    TVRYRCGQQL AIEAPVDADL VSTVPESATP AALAYAGKCG LPYVEVLCKN 350
    RYVGRTFIQP NMRLRQLGVA KKFGVLSDNF KGKRIVLVDD SIVRGNTISP 400
    IIKLLKESGA KEVHIRVASP PIKYPCFMGI NIPTKEELIA NKPEFDHLAE 450
    YLGANSVVYL SVEGLVSSVQ EGIKFKKQKE KKHDIMIQEN GNGLECFEKS 500
    GHCTACLTGK YPVELEW 517
    Length:517
    Mass (Da):57,399
    Last modified:June 1, 1994 - v1
    Checksum:iD3F2A354C36B29D9
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti369 – 3691V → I in AAC27345. (PubMed:8106516)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D13757 mRNA. Translation: BAA02903.1.
    U00238 mRNA. Translation: AAC27345.1.
    U00239 Genomic DNA. No translation available.
    BC004200 mRNA. Translation: AAH04200.1.
    CCDSiCCDS3505.1.
    PIRiA53342.
    RefSeqiNP_002694.3. NM_002703.4.
    UniGeneiHs.331420.

    Genome annotation databases

    EnsembliENST00000264220; ENSP00000264220; ENSG00000128059.
    GeneIDi5471.
    KEGGihsa:5471.
    UCSCiuc003hbr.3. human.

    Polymorphism databases

    DMDMi548638.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D13757 mRNA. Translation: BAA02903.1 .
    U00238 mRNA. Translation: AAC27345.1 .
    U00239 Genomic DNA. No translation available.
    BC004200 mRNA. Translation: AAH04200.1 .
    CCDSi CCDS3505.1.
    PIRi A53342.
    RefSeqi NP_002694.3. NM_002703.4.
    UniGenei Hs.331420.

    3D structure databases

    ProteinModelPortali Q06203.
    SMRi Q06203. Positions 12-515.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 111467. 19 interactions.
    IntActi Q06203. 2 interactions.
    STRINGi 9606.ENSP00000264220.

    Chemistry

    ChEMBLi CHEMBL2362992.
    DrugBanki DB00130. L-Glutamine.
    DB00352. Thioguanine.
    GuidetoPHARMACOLOGYi 2761.

    Protein family/group databases

    MEROPSi C44.001.

    PTM databases

    PhosphoSitei Q06203.

    Polymorphism databases

    DMDMi 548638.

    Proteomic databases

    MaxQBi Q06203.
    PaxDbi Q06203.
    PeptideAtlasi Q06203.
    PRIDEi Q06203.

    Protocols and materials databases

    DNASUi 5471.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000264220 ; ENSP00000264220 ; ENSG00000128059 .
    GeneIDi 5471.
    KEGGi hsa:5471.
    UCSCi uc003hbr.3. human.

    Organism-specific databases

    CTDi 5471.
    GeneCardsi GC04M057259.
    HGNCi HGNC:9238. PPAT.
    HPAi HPA036091.
    HPA036092.
    MIMi 172450. gene.
    neXtProti NX_Q06203.
    PharmGKBi PA33559.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG0034.
    HOGENOMi HOG000033688.
    HOVERGENi HBG002589.
    InParanoidi Q06203.
    KOi K00764.
    OMAi IPVGDMN.
    OrthoDBi EOG789C9W.
    PhylomeDBi Q06203.
    TreeFami TF106370.

    Enzyme and pathway databases

    UniPathwayi UPA00074 ; UER00124 .
    BioCyci MetaCyc:HS05157-MONOMER.
    Reactomei REACT_1776. Purine ribonucleoside monophosphate biosynthesis.

    Miscellaneous databases

    GeneWikii Amidophosphoribosyltransferase.
    GenomeRNAii 5471.
    NextBioi 21182.
    PROi Q06203.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q06203.
    Bgeei Q06203.
    CleanExi HS_PPAT.
    Genevestigatori Q06203.

    Family and domain databases

    Gene3Di 3.40.50.2020. 1 hit.
    3.60.20.10. 1 hit.
    InterProi IPR005854. Amd_phspho_trans.
    IPR017932. GATase_2_dom.
    IPR000583. GATase_dom.
    IPR029055. Ntn_hydrolases_N.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    [Graphical view ]
    Pfami PF00310. GATase_2. 2 hits.
    PF00156. Pribosyltran. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000485. Amd_phspho_trans. 1 hit.
    SUPFAMi SSF53271. SSF53271. 1 hit.
    SSF56235. SSF56235. 1 hit.
    TIGRFAMsi TIGR01134. purF. 1 hit.
    PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
    PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Hepatoma.
    2. "Two genes for de novo purine nucleotide synthesis on human chromosome 4 are closely linked and divergently transcribed."
      Brayton K.A., Chen Z., Zhou G., Nagy P.L., Gavalas A., Trent J.M., Deaven L.L., Dixon J.E., Zalkin H.
      J. Biol. Chem. 269:5313-5321(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Muscle.
    4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    5. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiPUR1_HUMAN
    AccessioniPrimary (citable) accession number: Q06203
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 147 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 4
      Human chromosome 4: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3