Reviewed, UniProtKB/Swiss-Prot Q06197 (IDHC_SOYBN)

Names and origin

Protein names

Recommended name:
    Isocitrate dehydrogenase [NADP]
      Short name=IDH
    EC=1.1.1.42
Alternative name(s):
    Oxalosuccinate decarboxylase
    NADP(+)-specific ICDH
    IDP

Gene names

Name:

IDH1

Organism

Glycine max (Soybean)

Taxonomic identifier

3847 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Fabales › Fabaceae › Papilionoideae › Phaseoleae › Glycine

Protein attributes

Sequence length	413 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	May supply 2-oxoglutarate for amino acid biosynthesis and ammonia assimilation.
Catalytic activity	Isocitrate + NADP(+) = 2-oxoglutarate + CO(2) + NADPH. Oxalosuccinate + NADP(+) = 2-oxoglutarate + CO(2) + NADPH.
Cofactor	Binds 1 magnesium or manganese ion per subunit.
Subunit structure	Heterodimer.
Subcellular location	Cytoplasm.
Tissue specificity	Leaves, nodules and roots with the relative amount of 1:3.4:7.7.
Sequence similarities	Belongs to the isocitrate and isopropylmalate dehydrogenases family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Ligand	Magnesium Manganese Metal-binding NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-KW isocitrate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	isocitrate dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: InterPro magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 413	413	Isocitrate dehydrogenase [NADP]		PRO_0000083585
Regions
	Nucleotide binding	78 – 80	3	NADP By similarity
	Nucleotide binding	313 – 318	6	NADP By similarity
	Region	97 – 103	7	Substrate binding By similarity
Sites
	Metal binding	255	1	Magnesium or manganese By similarity
	Metal binding	278	1	Magnesium or manganese By similarity
	Binding site	80	1	Substrate By similarity
	Binding site	85	1	NADP By similarity
	Binding site	112	1	Substrate By similarity
	Binding site	135	1	Substrate By similarity
	Binding site	263	1	NADP By similarity
	Binding site	331	1	NADP; via amide nitrogen and carbonyl oxygen By similarity
	Site	142	1	Critical for catalysis By similarity
	Site	215	1	Critical for catalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q06197-1 [UniParc]. Last modified October 1, 1996. Version 2. Checksum: 78B90E53C6D686B5	FASTA	413	46,050
	10 20 30 40 50 60 MAAFQKIKVA NPIVEMDGDE MTRVIWKSIK DKLILPFLEL DIKYYDLGLP YRDETDDKVT 70 80 90 100 110 120 IESAEATLKY NVAIKCATIT PDEARVKEFG LKSMWKSPNG TIRNILNGTV FREPILCKNI 130 140 150 160 170 180 PRLVPGWTKA ICIGRHAFGD QYRATDTVIK GAGKLKLVFV PEGQGEETEF EVFNFTGEGG 190 200 210 220 230 240 VSLAMYNTDE SIRSFAEASM ATALEKKWPL YLSTKNTILK KYDGRFKDIF QEVYEASWKS 250 260 270 280 290 300 KFEAAGIWYE HRLIDDMVAY ALKSEGGYVW ACKNYDGDVQ SDFLAQGFGS LGLMTSVLVC 310 320 330 340 350 360 PDGKTIEAEA AHGTVTRHFR VHQKGGETST NSIASIFAWT RGLAHRAKLD DNAKLLDFTE 370 380 390 400 410 KLEAACIGVV EAGKMTKDLA LILHGSKLSR EHYLNTEEFI DAVAAELSAR LSA

References

[1]

"Isolation and characterization of a cDNA encoding NADP(+)-specific isocitrate dehydrogenase from soybean (Glycine max)." Udvardi M.K., McDermott T.R., Kahn M.L. Plant Mol. Biol. 21:739-752(1993) [PubMed: 8467073] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Harosoy-L1. Tissue: Root nodule.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L12157 mRNA. Translation: AAA33978.1. Different initiation.
3D structure databases
HSSP	HSSP built from PDB template 1LWD based on UniProtKB P33198.
ModBase	Search...
Family and domain databases
InterPro	IPR004790. IsoCit_DHase_NADP-dep_euk. IPR001804. IsoCit_IM_DHase. [Graphical view]
Gene3D	G3DSA:3.40.718.10. IDH_IMDH. 1 hit.
PANTHER	PTHR11822. IDH_NADP_euk. 1 hit.
Pfam	PF00180. Iso_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000108. IDH_NADP. 1 hit.
ProDom	PD328597. Nadp_idh_plant. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00127. nadp_idh_euk. 1 hit.
PROSITE	PS00470. IDH_IMDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IDHC_SOYBN

Accession

Primary (citable) accession number: Q06197

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	October 1, 1996
Last modified:	November 25, 2008
This is version 55 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)