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Q06197 (IDHC_SOYBN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Isocitrate dehydrogenase [NADP]
Short name=IDH
EC=1.1.1.42
Alternative name(s):
IDP
NADP(+)-specific ICDH
Oxalosuccinate decarboxylase
Gene names
Name:IDH1
OrganismGlycine max (Soybean) (Glycine hispida) [Reference proteome]
Taxonomic identifier3847 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Phaseoleae › Glycine

Protein attributes

Sequence length413 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May supply 2-oxoglutarate for amino acid biosynthesis and ammonia assimilation via the glutamine synthetase/glutamate synthase (GS/GOGAT) pathway By similarity.

Catalytic activity

Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.

Cofactor

Binds 1 magnesium or manganese ion per subunit.

Subunit structure

Heterodimer.

Subcellular location

Cytoplasm.

Tissue specificity

Leaves, nodules and roots with the relative amount of 1:3.4:7.7.

Sequence similarities

Belongs to the isocitrate and isopropylmalate dehydrogenases family.

Sequence caution

The sequence AAA33978.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 413413Isocitrate dehydrogenase [NADP]
PRO_0000083585

Regions

Nucleotide binding78 – 803NADP By similarity
Nucleotide binding313 – 3186NADP By similarity
Region97 – 1037Substrate binding By similarity

Sites

Metal binding2551Magnesium or manganese By similarity
Metal binding2781Magnesium or manganese By similarity
Binding site801Substrate By similarity
Binding site851NADP By similarity
Binding site1121Substrate By similarity
Binding site1351Substrate By similarity
Binding site2631NADP By similarity
Binding site3311NADP; via amide nitrogen and carbonyl oxygen By similarity
Site1421Critical for catalysis By similarity
Site2151Critical for catalysis By similarity

Sequences

Sequence LengthMass (Da)Tools
Q06197 [UniParc].

Last modified October 1, 1996. Version 2.
Checksum: 78B90E53C6D686B5

FASTA41346,050
        10         20         30         40         50         60 
MAAFQKIKVA NPIVEMDGDE MTRVIWKSIK DKLILPFLEL DIKYYDLGLP YRDETDDKVT 

        70         80         90        100        110        120 
IESAEATLKY NVAIKCATIT PDEARVKEFG LKSMWKSPNG TIRNILNGTV FREPILCKNI 

       130        140        150        160        170        180 
PRLVPGWTKA ICIGRHAFGD QYRATDTVIK GAGKLKLVFV PEGQGEETEF EVFNFTGEGG 

       190        200        210        220        230        240 
VSLAMYNTDE SIRSFAEASM ATALEKKWPL YLSTKNTILK KYDGRFKDIF QEVYEASWKS 

       250        260        270        280        290        300 
KFEAAGIWYE HRLIDDMVAY ALKSEGGYVW ACKNYDGDVQ SDFLAQGFGS LGLMTSVLVC 

       310        320        330        340        350        360 
PDGKTIEAEA AHGTVTRHFR VHQKGGETST NSIASIFAWT RGLAHRAKLD DNAKLLDFTE 

       370        380        390        400        410 
KLEAACIGVV EAGKMTKDLA LILHGSKLSR EHYLNTEEFI DAVAAELSAR LSA

« Hide

References

[1]"Isolation and characterization of a cDNA encoding NADP(+)-specific isocitrate dehydrogenase from soybean (Glycine max)."
Udvardi M.K., McDermott T.R., Kahn M.L.
Plant Mol. Biol. 21:739-752(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Harosoy-L1.
Tissue: Root nodule.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		L12157 mRNA. Translation: AAA33978.1. Different initiation.
RefSeqXP_003544966.1. XM_003544918.1.
UniGeneGma.8359.

3D structure databases

ProteinModelPortalQ06197.
SMRQ06197. Positions 3-411.
ModBaseSearch...

Proteomic databases

PRIDEQ06197.
ProMEXQ06197.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsGLYMA14G39160.2; GLYMA14G39160.2; GLYMA14G39160.
GeneID100791166.
KEGGgmx:100791166.

Phylogenomic databases

KOK00031.

Gene expression databases

GenevestigatorQ06197.

Family and domain databases

Gene3D3.40.718.10. 1 hit.
InterProIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERPTHR11822. PTHR11822. 1 hit.
PfamPF00180. Iso_dh. 1 hit.
[Graphical view]
PIRSFPIRSF000108. IDH_NADP. 1 hit.
TIGRFAMsTIGR00127. nadp_idh_euk. 1 hit.
PROSITEPS00470. IDH_IMDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameIDHC_SOYBN
AccessionPrimary (citable) accession number: Q06197
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: October 1, 1996
Last modified: April 3, 2013
This is version 83 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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