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Q06197

- IDHC_SOYBN

UniProt

Q06197 - IDHC_SOYBN

Protein

Isocitrate dehydrogenase [NADP]

Gene

IDH1

Organism
Glycine max (Soybean) (Glycine hispida)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 89 (01 Oct 2014)
      Sequence version 2 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    May supply 2-oxoglutarate for amino acid biosynthesis and ammonia assimilation via the glutamine synthetase/glutamate synthase (GS/GOGAT) pathway.By similarity

    Catalytic activityi

    Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.

    Cofactori

    Binds 1 magnesium or manganese ion per subunit.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei80 – 801SubstrateBy similarity
    Binding sitei85 – 851NADPBy similarity
    Binding sitei112 – 1121SubstrateBy similarity
    Binding sitei135 – 1351SubstrateBy similarity
    Sitei142 – 1421Critical for catalysisBy similarity
    Sitei215 – 2151Critical for catalysisBy similarity
    Metal bindingi255 – 2551Magnesium or manganeseBy similarity
    Binding sitei263 – 2631NADPBy similarity
    Metal bindingi278 – 2781Magnesium or manganeseBy similarity
    Binding sitei331 – 3311NADP; via amide nitrogen and carbonyl oxygenBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi78 – 803NADPBy similarity
    Nucleotide bindingi313 – 3186NADPBy similarity

    GO - Molecular functioni

    1. isocitrate dehydrogenase (NADP+) activity Source: UniProtKB-EC
    2. magnesium ion binding Source: InterPro
    3. NAD binding Source: InterPro

    GO - Biological processi

    1. glyoxylate cycle Source: UniProtKB-KW
    2. isocitrate metabolic process Source: InterPro
    3. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Glyoxylate bypass, Tricarboxylic acid cycle

    Keywords - Ligandi

    Magnesium, Manganese, Metal-binding, NADP

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isocitrate dehydrogenase [NADP] (EC:1.1.1.42)
    Short name:
    IDH
    Alternative name(s):
    IDP
    NADP(+)-specific ICDH
    Oxalosuccinate decarboxylase
    Gene namesi
    Name:IDH1
    OrganismiGlycine max (Soybean) (Glycine hispida)
    Taxonomic identifieri3847 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaeGlycineSoja
    ProteomesiUP000008827: Chromosome 14

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 413413Isocitrate dehydrogenase [NADP]PRO_0000083585Add
    BLAST

    Proteomic databases

    PRIDEiQ06197.
    ProMEXiQ06197.

    Expressioni

    Tissue specificityi

    Leaves, nodules and roots with the relative amount of 1:3.4:7.7.

    Gene expression databases

    GenevestigatoriQ06197.

    Interactioni

    Subunit structurei

    Heterodimer.

    Structurei

    3D structure databases

    ProteinModelPortaliQ06197.
    SMRiQ06197. Positions 3-411.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni97 – 1037Substrate bindingBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    KOiK00031.
    OMAiGEGTLEM.

    Family and domain databases

    Gene3Di3.40.718.10. 1 hit.
    InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
    IPR004790. Isocitrate_DH_NADP.
    IPR024084. IsoPropMal-DH-like_dom.
    [Graphical view]
    PANTHERiPTHR11822. PTHR11822. 1 hit.
    PfamiPF00180. Iso_dh. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000108. IDH_NADP. 1 hit.
    TIGRFAMsiTIGR00127. nadp_idh_euk. 1 hit.
    PROSITEiPS00470. IDH_IMDH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q06197-1 [UniParc]FASTAAdd to Basket

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    MAAFQKIKVA NPIVEMDGDE MTRVIWKSIK DKLILPFLEL DIKYYDLGLP    50
    YRDETDDKVT IESAEATLKY NVAIKCATIT PDEARVKEFG LKSMWKSPNG 100
    TIRNILNGTV FREPILCKNI PRLVPGWTKA ICIGRHAFGD QYRATDTVIK 150
    GAGKLKLVFV PEGQGEETEF EVFNFTGEGG VSLAMYNTDE SIRSFAEASM 200
    ATALEKKWPL YLSTKNTILK KYDGRFKDIF QEVYEASWKS KFEAAGIWYE 250
    HRLIDDMVAY ALKSEGGYVW ACKNYDGDVQ SDFLAQGFGS LGLMTSVLVC 300
    PDGKTIEAEA AHGTVTRHFR VHQKGGETST NSIASIFAWT RGLAHRAKLD 350
    DNAKLLDFTE KLEAACIGVV EAGKMTKDLA LILHGSKLSR EHYLNTEEFI 400
    DAVAAELSAR LSA 413
    Length:413
    Mass (Da):46,050
    Last modified:October 1, 1996 - v2
    Checksum:i78B90E53C6D686B5
    GO

    Sequence cautioni

    The sequence AAA33978.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L12157 mRNA. Translation: AAA33978.1. Different initiation.
    RefSeqiXP_006596496.1. XM_006596433.1.
    UniGeneiGma.8359.

    Genome annotation databases

    EnsemblPlantsiGLYMA14G39160.2; GLYMA14G39160.2; GLYMA14G39160.
    GeneIDi100791166.
    KEGGigmx:100791166.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L12157 mRNA. Translation: AAA33978.1 . Different initiation.
    RefSeqi XP_006596496.1. XM_006596433.1.
    UniGenei Gma.8359.

    3D structure databases

    ProteinModelPortali Q06197.
    SMRi Q06197. Positions 3-411.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi Q06197.
    ProMEXi Q06197.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi GLYMA14G39160.2 ; GLYMA14G39160.2 ; GLYMA14G39160 .
    GeneIDi 100791166.
    KEGGi gmx:100791166.

    Phylogenomic databases

    KOi K00031.
    OMAi GEGTLEM.

    Gene expression databases

    Genevestigatori Q06197.

    Family and domain databases

    Gene3Di 3.40.718.10. 1 hit.
    InterProi IPR019818. IsoCit/isopropylmalate_DH_CS.
    IPR004790. Isocitrate_DH_NADP.
    IPR024084. IsoPropMal-DH-like_dom.
    [Graphical view ]
    PANTHERi PTHR11822. PTHR11822. 1 hit.
    Pfami PF00180. Iso_dh. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000108. IDH_NADP. 1 hit.
    TIGRFAMsi TIGR00127. nadp_idh_euk. 1 hit.
    PROSITEi PS00470. IDH_IMDH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and characterization of a cDNA encoding NADP(+)-specific isocitrate dehydrogenase from soybean (Glycine max)."
      Udvardi M.K., McDermott T.R., Kahn M.L.
      Plant Mol. Biol. 21:739-752(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Harosoy-L1.
      Tissue: Root nodule.

    Entry informationi

    Entry nameiIDHC_SOYBN
    AccessioniPrimary (citable) accession number: Q06197
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 89 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3