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Protein

Isocitrate dehydrogenase [NADP]

Gene

IDH1

Organism
Glycine max (Soybean) (Glycine hispida)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May supply 2-oxoglutarate for amino acid biosynthesis and ammonia assimilation via the glutamine synthetase/glutamate synthase (GS/GOGAT) pathway.By similarity

Catalytic activityi

Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.

Cofactori

Mg2+, Mn2+Note: Binds 1 Mg(2+) or Mn2+ ion per subunit.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei80 – 801SubstrateBy similarity
Binding sitei85 – 851NADPBy similarity
Binding sitei112 – 1121SubstrateBy similarity
Binding sitei135 – 1351SubstrateBy similarity
Sitei142 – 1421Critical for catalysisBy similarity
Sitei215 – 2151Critical for catalysisBy similarity
Metal bindingi255 – 2551Magnesium or manganeseBy similarity
Binding sitei263 – 2631NADPBy similarity
Metal bindingi278 – 2781Magnesium or manganeseBy similarity
Binding sitei331 – 3311NADP; via amide nitrogen and carbonyl oxygenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi78 – 803NADPBy similarity
Nucleotide bindingi313 – 3186NADPBy similarity

GO - Molecular functioni

  1. isocitrate dehydrogenase (NADP+) activity Source: UniProtKB-EC
  2. magnesium ion binding Source: InterPro
  3. NAD binding Source: InterPro

GO - Biological processi

  1. glyoxylate cycle Source: UniProtKB-KW
  2. isocitrate metabolic process Source: InterPro
  3. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Manganese, Metal-binding, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate dehydrogenase [NADP] (EC:1.1.1.42)
Short name:
IDH
Alternative name(s):
IDP
NADP(+)-specific ICDH
Oxalosuccinate decarboxylase
Gene namesi
Name:IDH1
OrganismiGlycine max (Soybean) (Glycine hispida)
Taxonomic identifieri3847 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaeGlycineSoja
ProteomesiUP000008827: Chromosome 14

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 413413Isocitrate dehydrogenase [NADP]PRO_0000083585Add
BLAST

Proteomic databases

PRIDEiQ06197.
ProMEXiQ06197.

Expressioni

Tissue specificityi

Leaves, nodules and roots with the relative amount of 1:3.4:7.7.

Gene expression databases

GenevestigatoriQ06197.

Interactioni

Subunit structurei

Heterodimer.

Structurei

3D structure databases

ProteinModelPortaliQ06197.
SMRiQ06197. Positions 3-411.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni97 – 1037Substrate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

InParanoidiQ06197.
KOiK00031.
OMAiQAGHETS.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11822. PTHR11822. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF000108. IDH_NADP. 1 hit.
TIGRFAMsiTIGR00127. nadp_idh_euk. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q06197-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAFQKIKVA NPIVEMDGDE MTRVIWKSIK DKLILPFLEL DIKYYDLGLP
60 70 80 90 100
YRDETDDKVT IESAEATLKY NVAIKCATIT PDEARVKEFG LKSMWKSPNG
110 120 130 140 150
TIRNILNGTV FREPILCKNI PRLVPGWTKA ICIGRHAFGD QYRATDTVIK
160 170 180 190 200
GAGKLKLVFV PEGQGEETEF EVFNFTGEGG VSLAMYNTDE SIRSFAEASM
210 220 230 240 250
ATALEKKWPL YLSTKNTILK KYDGRFKDIF QEVYEASWKS KFEAAGIWYE
260 270 280 290 300
HRLIDDMVAY ALKSEGGYVW ACKNYDGDVQ SDFLAQGFGS LGLMTSVLVC
310 320 330 340 350
PDGKTIEAEA AHGTVTRHFR VHQKGGETST NSIASIFAWT RGLAHRAKLD
360 370 380 390 400
DNAKLLDFTE KLEAACIGVV EAGKMTKDLA LILHGSKLSR EHYLNTEEFI
410
DAVAAELSAR LSA
Length:413
Mass (Da):46,050
Last modified:October 1, 1996 - v2
Checksum:i78B90E53C6D686B5
GO

Sequence cautioni

The sequence AAA33978.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L12157 mRNA. Translation: AAA33978.1. Different initiation.
RefSeqiXP_006596496.1. XM_006596433.1.
UniGeneiGma.8359.

Genome annotation databases

EnsemblPlantsiGLYMA14G39160.2; GLYMA14G39160.2; GLYMA14G39160.
GeneIDi100791166.
KEGGigmx:100791166.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L12157 mRNA. Translation: AAA33978.1. Different initiation.
RefSeqiXP_006596496.1. XM_006596433.1.
UniGeneiGma.8359.

3D structure databases

ProteinModelPortaliQ06197.
SMRiQ06197. Positions 3-411.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ06197.
ProMEXiQ06197.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiGLYMA14G39160.2; GLYMA14G39160.2; GLYMA14G39160.
GeneIDi100791166.
KEGGigmx:100791166.

Phylogenomic databases

InParanoidiQ06197.
KOiK00031.
OMAiQAGHETS.

Gene expression databases

GenevestigatoriQ06197.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11822. PTHR11822. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF000108. IDH_NADP. 1 hit.
TIGRFAMsiTIGR00127. nadp_idh_euk. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Isolation and characterization of a cDNA encoding NADP(+)-specific isocitrate dehydrogenase from soybean (Glycine max)."
    Udvardi M.K., McDermott T.R., Kahn M.L.
    Plant Mol. Biol. 21:739-752(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Harosoy-L1.
    Tissue: Root nodule.

Entry informationi

Entry nameiIDHC_SOYBN
AccessioniPrimary (citable) accession number: Q06197
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: October 1, 1996
Last modified: January 7, 2015
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.