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Q06194 (FA8_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 123. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Coagulation factor VIII
Alternative name(s):
Procoagulant component
Gene names
Name:F8
Synonyms:Cf8, F8c
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length2319 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Factor VIII, along with calcium and phospholipid, acts as a cofactor for factor IXa when it converts factor X to the activated form, factor Xa.

Subunit structure

Interacts with vWF. vWF binding is essential for the stabilization of F8 in circulation By similarity.

Subcellular location

Secretedextracellular space.

Tissue specificity

Found in most tissues.

Post-translational modification

The binding of vWF and activation depend on the sulfation of Tyr-1669.

Sequence similarities

Belongs to the multicopper oxidase family.

Contains 3 F5/8 type A domains.

Contains 2 F5/8 type C domains.

Contains 6 plastocyanin-like domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 Potential
Chain20 – 23192300Coagulation factor VIII
PRO_0000002972

Regions

Domain20 – 349330F5/8 type A 1
Domain20 – 199180Plastocyanin-like 1
Domain207 – 349143Plastocyanin-like 2
Domain399 – 730332F5/8 type A 2
Domain399 – 573175Plastocyanin-like 3
Domain583 – 730148Plastocyanin-like 4
Domain1683 – 2008326F5/8 type A 3
Domain1683 – 1845163Plastocyanin-like 5
Domain1855 – 2008154Plastocyanin-like 6
Domain2008 – 2156149F5/8 type C 1
Domain2161 – 2313153F5/8 type C 2
Region760 – 1640881B

Sites

Site391 – 3922Cleavage; by thrombin By similarity
Site759 – 7602Cleavage; by thrombin By similarity
Site1324 – 13252Cleavage (activation) By similarity
Site1640 – 16412Cleavage (activation) By similarity
Site1678 – 16792Cleavage; by thrombin By similarity

Amino acid modifications

Modified residue3671Sulfotyrosine By similarity
Modified residue7371Sulfotyrosine By similarity
Modified residue7381Sulfotyrosine By similarity
Modified residue7421Sulfotyrosine By similarity
Modified residue16691Sulfotyrosine By similarity
Modified residue16871Sulfotyrosine By similarity
Glycosylation611N-linked (GlcNAc...) Potential
Glycosylation2331N-linked (GlcNAc...) Potential
Glycosylation2591N-linked (GlcNAc...) Potential
Glycosylation4231N-linked (GlcNAc...) Potential
Glycosylation6011N-linked (GlcNAc...) Potential
Glycosylation8801N-linked (GlcNAc...) Potential
Glycosylation9581N-linked (GlcNAc...) Potential
Glycosylation10151N-linked (GlcNAc...) Potential
Glycosylation10221N-linked (GlcNAc...) Potential
Glycosylation10261N-linked (GlcNAc...) Potential
Glycosylation10441N-linked (GlcNAc...) Potential
Glycosylation10761N-linked (GlcNAc...) Potential
Glycosylation10871N-linked (GlcNAc...) Potential
Glycosylation11361N-linked (GlcNAc...) Potential
Glycosylation11611N-linked (GlcNAc...) Potential
Glycosylation11921N-linked (GlcNAc...) Potential
Glycosylation12551N-linked (GlcNAc...) Potential
Glycosylation12681N-linked (GlcNAc...) Potential
Glycosylation12731N-linked (GlcNAc...) Potential
Glycosylation12741N-linked (GlcNAc...) Potential
Glycosylation13021N-linked (GlcNAc...) Potential
Glycosylation13161N-linked (GlcNAc...) Potential
Glycosylation13401N-linked (GlcNAc...) Potential
Glycosylation13781N-linked (GlcNAc...) Potential
Glycosylation17971N-linked (GlcNAc...) Potential
Glycosylation21051N-linked (GlcNAc...) Potential
Disulfide bond173 ↔ 199 Probable
Disulfide bond547 ↔ 573 Probable
Disulfide bond1819 ↔ 1845 Probable
Disulfide bond2008 ↔ 2156 By similarity
Disulfide bond2161 ↔ 2313 By similarity

Experimental info

Sequence conflict9591V → A in AAA37385. Ref.1
Sequence conflict9881M → I in AAA37385. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q06194 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 09CAC4CFBCCECA57

FASTA2,319266,196
        10         20         30         40         50         60 
MQIALFACFF LSLFNFCSSA IRRYYLGAVE LSWNYIQSDL LSVLHTDSRF LPRMSTSFPF 

        70         80         90        100        110        120 
NTSIMYKKTV FVEYKDQLFN IAKPRPPWMG LLGPTIWTEV HDTVVITLKN MASHPVSLHA 

       130        140        150        160        170        180 
VGVSYWKASE GDEYEDQTSQ MEKEDDKVFP GESHTYVWQV LKENGPMASD PPCLTYSYMS 

       190        200        210        220        230        240 
HVDLVKDLNS GLIGALLVCK EGSLSKERTQ MLYQFVLLFA VFDEGKSWHS ETNDSYTQSM 

       250        260        270        280        290        300 
DSASARDWPK MHTVNGYVNR SLPGLIGCHR KSVYWHVIGM GTTPEIHSIF LEGHTFFVRN 

       310        320        330        340        350        360 
HRQASLEISP ITFLTAQTLL IDLGQFLLFC HISSHKHDGM EAYVKVDSCP EESQWQKKNN 

       370        380        390        400        410        420 
NEEMEDYDDD LYSEMDMFTL DYDSSPFIQI RSVAKKYPKT WIHYISAEEE DWDYAPSVPT 

       430        440        450        460        470        480 
SDNGSYKSQY LSNGPHRIGR KYKKVRFIAY TDETFKTRET IQHESGLLGP LLYGEVGDTL 

       490        500        510        520        530        540 
LIIFKNQASR PYNIYPHGIT DVSPLHARRL PRGIKHVKDL PIHPGEIFKY KWTVTVEDGP 

       550        560        570        580        590        600 
TKSDPRCLTR YYSSFINPER DLASGLIGPL LICYKESVDQ RGNQMMSDKR NVILFSIFDE 

       610        620        630        640        650        660 
NQSWYITENM QRFLPNAAKT QPQDPGFQAS NIMHSINGYV FDSLELTVCL HEVAYWHILS 

       670        680        690        700        710        720 
VGAQTDFLSI FFSGYTFKHK MVYEDTLTLF PFSGETVFMS MENPGLWVLG CHNSDFRKRG 

       730        740        750        760        770        780 
MTALLKVSSC DKSTSDYYEE IYEDIPTQLV NENNVIDPRS FFQNTNHPNT RKKKFKDSTI 

       790        800        810        820        830        840 
PKNDMEKIEP QFEEIAEMLK VQSVSVSDML MLLGQSHPTP HGLFLSDGQE AIYEAIHDDH 

       850        860        870        880        890        900 
SPNAIDSNEG PSKVTQLRPE SHHSEKIVFT PQPGLQLRSN KSLETTIEVK WKKLGLQVSS 

       910        920        930        940        950        960 
LPSNLMTTTI LSDNLKATFE KTDSSGFPDM PVHSSSKLST TAFGKKAYSL VGSHVPLNVS 

       970        980        990       1000       1010       1020 
EENSDSNILD STLMYSQESL PRDNILSMEN DRLLREKRFH GIALLTKDNT LFKDNVSLMK 

      1030       1040       1050       1060       1070       1080 
TNKTYNHSTT NEKLHTESPT SIENSTTDLQ DAILKVNSEI QEVTALIHDG TLLGKNSTYL 

      1090       1100       1110       1120       1130       1140 
RLNHMLNRTT STKNKDIFHR KDEDPIPQDE ENTIMPFSKM LFLSESSNWF KKTNGNNSLN 

      1150       1160       1170       1180       1190       1200 
SEQEHSPKQL VYLMFKKYVK NQSFLSEKNK VTVEQDGFTK NIGLKDMAFP HNMSIFLTTL 

      1210       1220       1230       1240       1250       1260 
SNVHENGRHN QEKNIQEEIE KEALIEEKVV LPQVHEATGS KNFLKDILIL GTRQNISLYE 

      1270       1280       1290       1300       1310       1320 
VHVPVLQNIT SINNSTNTVQ IHMEHFFKRR KDKETNSEGL VNKTREMVKN YPSQKNITTQ 

      1330       1340       1350       1360       1370       1380 
RSKRALGQFR LSTQWLKTIN CSTQCIIKQI DHSKEMKKFI TKSSLSDSSV IKSTTQTNSS 

      1390       1400       1410       1420       1430       1440 
DSHIVKTSAF PPIDLKRSPF QNKFSHVQAS SYIYDFKTKS SRIQESNNFL KETKINNPSL 

      1450       1460       1470       1480       1490       1500 
AILPWNMFID QGKFTSPGKS NTNSVTYKKR ENIIFLKPTL PEESGKIELL PQVSIQEEEI 

      1510       1520       1530       1540       1550       1560 
LPTETSHGSP GHLNLMKEVF LQKIQGPTKW NKAKRHGESI KGKTESSKNT RSKLLNHHAW 

      1570       1580       1590       1600       1610       1620 
DYHYAAQIPK DMWKSKEKSP EIISIKQEDT ILSLRPHGNS HSIGANEKQN WPQRETTWVK 

      1630       1640       1650       1660       1670       1680 
QGQTQRTCSQ IPPVLKRHQR ELSAFQSEQE ATDYDDAITI ETIEDFDIYS EDIKQGPRSF 

      1690       1700       1710       1720       1730       1740 
QQKTRHYFIA AVERLWDYGM STSHVLRNRY QSDNVPQFKK VVFQEFTDGS FSQPLYRGEL 

      1750       1760       1770       1780       1790       1800 
NEHLGLLGPY IRAEVEDNIM VTFKNQASRP YSFYSSLISY KEDQRGEEPR RNFVKPNETK 

      1810       1820       1830       1840       1850       1860 
IYFWKVQHHM APTEDEFDCK AWAYFSDVDL ERDMHSGLIG PLLICHANTL NPAHGRQVSV 

      1870       1880       1890       1900       1910       1920 
QEFALLFTIF DETKSWYFTE NVKRNCKTPC NFQMEDPTLK ENYRFHAING YVMDTLPGLV 

      1930       1940       1950       1960       1970       1980 
MAQDQRIRWY LLSMGNNENI QSIHFSGHVF TVRKKEEYKM AVYNLYPGVF ETLEMIPSRA 

      1990       2000       2010       2020       2030       2040 
GIWRVECLIG EHLQAGMSTL FLVYSKQCQI PLGMASGSIR DFQITASGHY GQWAPNLARL 

      2050       2060       2070       2080       2090       2100 
HYSGSINAWS TKEPFSWIKV DLLAPMIVHG IKTQGARQKF SSLYISQFII MYSLDGKKWL 

      2110       2120       2130       2140       2150       2160 
SYQGNSTGTL MVFFGNVDSS GIKHNSFNPP IIARYIRLHP THSSIRSTLR MELMGCDLNS 

      2170       2180       2190       2200       2210       2220 
CSIPLGMESK VISDTQITAS SYFTNMFATW SPSQARLHLQ GRTNAWRPQV NDPKQWLQVD 

      2230       2240       2250       2260       2270       2280 
LQKTMKVTGI ITQGVKSLFT SMFVKEFLIS SSQDGHHWTQ ILYNGKVKVF QGNQDSSTPM 

      2290       2300       2310 
MNSLDPPLLT RYLRIHPQIW EHQIALRLEI LGCEAQQQY 

« Hide

References

« Hide 'large scale' references
[1]"Sequence of the murine factor VIII cDNA."
Elder B., Lakich D., Gitschier J.
Genomics 16:374-379(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C57BL/6 X CBA.
Tissue: Liver.
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L05573 mRNA. Translation: AAA37385.1.
AL731844, AL808110 Genomic DNA. Translation: CAM15581.1.
AL808110, AL731844 Genomic DNA. Translation: CAM26492.1.
CH466576 Genomic DNA. Translation: EDL29229.1.
CCDSCCDS30238.1.
PIRA47004.
RefSeqNP_032003.2. NM_007977.2.
UniGeneMm.1805.

3D structure databases

ProteinModelPortalQ06194.
SMRQ06194. Positions 20-744, 1678-2319.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ06194.

Proteomic databases

PaxDbQ06194.
PRIDEQ06194.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000033539; ENSMUSP00000033539; ENSMUSG00000031196.
GeneID14069.
KEGGmmu:14069.
UCSCuc009tpt.3. mouse.

Organism-specific databases

CTD2157.
MGIMGI:88383. F8.

Phylogenomic databases

eggNOGNOG151351.
GeneTreeENSGT00740000114988.
HOGENOMHOG000231686.
HOVERGENHBG106657.
InParanoidA2AN88.
KOK03899.
OMAWHVIGMG.
OrthoDBEOG7ZWD1D.
TreeFamTF329807.

Gene expression databases

ArrayExpressQ06194.
BgeeQ06194.
CleanExMM_F8.
GenevestigatorQ06194.

Family and domain databases

Gene3D2.60.120.260. 2 hits.
2.60.40.420. 6 hits.
InterProIPR000421. Coagulation_fac_5/8-C_type_dom.
IPR011706. Cu-oxidase_2.
IPR011707. Cu-oxidase_3.
IPR002355. Cu_oxidase_Cu_BS.
IPR008972. Cupredoxin.
IPR024715. Factor_5/8.
IPR014707. Factor_8.
IPR008979. Galactose-bd-like.
[Graphical view]
PANTHERPTHR10127:SF50. PTHR10127:SF50. 1 hit.
PfamPF07731. Cu-oxidase_2. 1 hit.
PF07732. Cu-oxidase_3. 2 hits.
PF00754. F5_F8_type_C. 2 hits.
[Graphical view]
PIRSFPIRSF000354. Factors_V_VIII. 1 hit.
SMARTSM00231. FA58C. 2 hits.
[Graphical view]
SUPFAMSSF49503. SSF49503. 6 hits.
SSF49785. SSF49785. 2 hits.
PROSITEPS01285. FA58C_1. 2 hits.
PS01286. FA58C_2. 2 hits.
PS50022. FA58C_3. 2 hits.
PS00079. MULTICOPPER_OXIDASE1. 3 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio285060.
PROQ06194.
SOURCESearch...

Entry information

Entry nameFA8_MOUSE
AccessionPrimary (citable) accession number: Q06194
Secondary accession number(s): A2AN88
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot