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Q06194

- FA8_MOUSE

UniProt

Q06194 - FA8_MOUSE

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Protein
Coagulation factor VIII
Gene
F8, Cf8, F8c
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Factor VIII, along with calcium and phospholipid, acts as a cofactor for factor IXa when it converts factor X to the activated form, factor Xa.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei391 – 3922Cleavage; by thrombin By similarity
Sitei759 – 7602Cleavage; by thrombin By similarity
Sitei1324 – 13252Cleavage (activation) By similarity
Sitei1640 – 16412Cleavage (activation) By similarity
Sitei1678 – 16792Cleavage; by thrombin By similarity

GO - Molecular functioni

  1. copper ion binding Source: InterPro
  2. oxidoreductase activity Source: InterPro
  3. serine-type endopeptidase activity Source: Ensembl
Complete GO annotation...

GO - Biological processi

  1. acute-phase response Source: UniProtKB-KW
  2. blood coagulation, intrinsic pathway Source: Ensembl
  3. cell adhesion Source: InterPro
  4. platelet activation Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Acute phase, Blood coagulation, Hemostasis

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Coagulation factor VIII
Alternative name(s):
Procoagulant component
Gene namesi
Name:F8
Synonyms:Cf8, F8c
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome X

Organism-specific databases

MGIiMGI:88383. F8.

Subcellular locationi

GO - Cellular componenti

  1. extracellular space Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919 Reviewed prediction
Add
BLAST
Chaini20 – 23192300Coagulation factor VIII
PRO_0000002972Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi61 – 611N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi173 ↔ 199 Inferred
Glycosylationi233 – 2331N-linked (GlcNAc...) Reviewed prediction
Glycosylationi259 – 2591N-linked (GlcNAc...) Reviewed prediction
Modified residuei367 – 3671Sulfotyrosine By similarity
Glycosylationi423 – 4231N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi547 ↔ 573 Inferred
Glycosylationi601 – 6011N-linked (GlcNAc...) Reviewed prediction
Modified residuei737 – 7371Sulfotyrosine By similarity
Modified residuei738 – 7381Sulfotyrosine By similarity
Modified residuei742 – 7421Sulfotyrosine By similarity
Glycosylationi880 – 8801N-linked (GlcNAc...) Reviewed prediction
Glycosylationi958 – 9581N-linked (GlcNAc...) Reviewed prediction
Glycosylationi1015 – 10151N-linked (GlcNAc...) Reviewed prediction
Glycosylationi1022 – 10221N-linked (GlcNAc...) Reviewed prediction
Glycosylationi1026 – 10261N-linked (GlcNAc...) Reviewed prediction
Glycosylationi1044 – 10441N-linked (GlcNAc...) Reviewed prediction
Glycosylationi1076 – 10761N-linked (GlcNAc...) Reviewed prediction
Glycosylationi1087 – 10871N-linked (GlcNAc...) Reviewed prediction
Glycosylationi1136 – 11361N-linked (GlcNAc...) Reviewed prediction
Glycosylationi1161 – 11611N-linked (GlcNAc...) Reviewed prediction
Glycosylationi1192 – 11921N-linked (GlcNAc...) Reviewed prediction
Glycosylationi1255 – 12551N-linked (GlcNAc...) Reviewed prediction
Glycosylationi1268 – 12681N-linked (GlcNAc...) Reviewed prediction
Glycosylationi1273 – 12731N-linked (GlcNAc...) Reviewed prediction
Glycosylationi1274 – 12741N-linked (GlcNAc...) Reviewed prediction
Glycosylationi1302 – 13021N-linked (GlcNAc...) Reviewed prediction
Glycosylationi1316 – 13161N-linked (GlcNAc...) Reviewed prediction
Glycosylationi1340 – 13401N-linked (GlcNAc...) Reviewed prediction
Glycosylationi1378 – 13781N-linked (GlcNAc...) Reviewed prediction
Modified residuei1669 – 16691Sulfotyrosine By similarity
Modified residuei1687 – 16871Sulfotyrosine By similarity
Glycosylationi1797 – 17971N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi1819 ↔ 1845 Inferred
Disulfide bondi2008 ↔ 2156 By similarity
Glycosylationi2105 – 21051N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi2161 ↔ 2313 By similarity

Post-translational modificationi

The binding of vWF and activation depend on the sulfation of Tyr-1669.

Keywords - PTMi

Disulfide bond, Glycoprotein, Sulfation

Proteomic databases

PaxDbiQ06194.
PRIDEiQ06194.

PTM databases

PhosphoSiteiQ06194.

Expressioni

Tissue specificityi

Found in most tissues.

Gene expression databases

ArrayExpressiQ06194.
BgeeiQ06194.
CleanExiMM_F8.
GenevestigatoriQ06194.

Interactioni

Subunit structurei

Interacts with vWF. vWF binding is essential for the stabilization of F8 in circulation By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ06194.
SMRiQ06194. Positions 20-744, 1678-2319.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini20 – 349330F5/8 type A 1
Add
BLAST
Domaini20 – 199180Plastocyanin-like 1
Add
BLAST
Domaini207 – 349143Plastocyanin-like 2
Add
BLAST
Domaini399 – 730332F5/8 type A 2
Add
BLAST
Domaini399 – 573175Plastocyanin-like 3
Add
BLAST
Domaini583 – 730148Plastocyanin-like 4
Add
BLAST
Domaini1683 – 2008326F5/8 type A 3
Add
BLAST
Domaini1683 – 1845163Plastocyanin-like 5
Add
BLAST
Domaini1855 – 2008154Plastocyanin-like 6
Add
BLAST
Domaini2008 – 2156149F5/8 type C 1
Add
BLAST
Domaini2161 – 2313153F5/8 type C 2
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni760 – 1640881B
Add
BLAST

Sequence similaritiesi

Contains 3 F5/8 type A domains.
Contains 2 F5/8 type C domains.

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiNOG151351.
GeneTreeiENSGT00740000114988.
HOGENOMiHOG000231686.
HOVERGENiHBG106657.
InParanoidiA2AN88.
KOiK03899.
OMAiWHVIGMG.
OrthoDBiEOG7ZWD1D.
TreeFamiTF329807.

Family and domain databases

Gene3Di2.60.120.260. 2 hits.
2.60.40.420. 6 hits.
InterProiIPR000421. Coagulation_fac_5/8-C_type_dom.
IPR011706. Cu-oxidase_2.
IPR011707. Cu-oxidase_3.
IPR002355. Cu_oxidase_Cu_BS.
IPR008972. Cupredoxin.
IPR024715. Factor_5/8.
IPR014707. Factor_8.
IPR008979. Galactose-bd-like.
[Graphical view]
PANTHERiPTHR10127:SF50. PTHR10127:SF50. 1 hit.
PfamiPF07731. Cu-oxidase_2. 1 hit.
PF07732. Cu-oxidase_3. 2 hits.
PF00754. F5_F8_type_C. 2 hits.
[Graphical view]
PIRSFiPIRSF000354. Factors_V_VIII. 1 hit.
SMARTiSM00231. FA58C. 2 hits.
[Graphical view]
SUPFAMiSSF49503. SSF49503. 6 hits.
SSF49785. SSF49785. 2 hits.
PROSITEiPS01285. FA58C_1. 2 hits.
PS01286. FA58C_2. 2 hits.
PS50022. FA58C_3. 2 hits.
PS00079. MULTICOPPER_OXIDASE1. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q06194-1 [UniParc]FASTAAdd to Basket

« Hide

MQIALFACFF LSLFNFCSSA IRRYYLGAVE LSWNYIQSDL LSVLHTDSRF     50
LPRMSTSFPF NTSIMYKKTV FVEYKDQLFN IAKPRPPWMG LLGPTIWTEV 100
HDTVVITLKN MASHPVSLHA VGVSYWKASE GDEYEDQTSQ MEKEDDKVFP 150
GESHTYVWQV LKENGPMASD PPCLTYSYMS HVDLVKDLNS GLIGALLVCK 200
EGSLSKERTQ MLYQFVLLFA VFDEGKSWHS ETNDSYTQSM DSASARDWPK 250
MHTVNGYVNR SLPGLIGCHR KSVYWHVIGM GTTPEIHSIF LEGHTFFVRN 300
HRQASLEISP ITFLTAQTLL IDLGQFLLFC HISSHKHDGM EAYVKVDSCP 350
EESQWQKKNN NEEMEDYDDD LYSEMDMFTL DYDSSPFIQI RSVAKKYPKT 400
WIHYISAEEE DWDYAPSVPT SDNGSYKSQY LSNGPHRIGR KYKKVRFIAY 450
TDETFKTRET IQHESGLLGP LLYGEVGDTL LIIFKNQASR PYNIYPHGIT 500
DVSPLHARRL PRGIKHVKDL PIHPGEIFKY KWTVTVEDGP TKSDPRCLTR 550
YYSSFINPER DLASGLIGPL LICYKESVDQ RGNQMMSDKR NVILFSIFDE 600
NQSWYITENM QRFLPNAAKT QPQDPGFQAS NIMHSINGYV FDSLELTVCL 650
HEVAYWHILS VGAQTDFLSI FFSGYTFKHK MVYEDTLTLF PFSGETVFMS 700
MENPGLWVLG CHNSDFRKRG MTALLKVSSC DKSTSDYYEE IYEDIPTQLV 750
NENNVIDPRS FFQNTNHPNT RKKKFKDSTI PKNDMEKIEP QFEEIAEMLK 800
VQSVSVSDML MLLGQSHPTP HGLFLSDGQE AIYEAIHDDH SPNAIDSNEG 850
PSKVTQLRPE SHHSEKIVFT PQPGLQLRSN KSLETTIEVK WKKLGLQVSS 900
LPSNLMTTTI LSDNLKATFE KTDSSGFPDM PVHSSSKLST TAFGKKAYSL 950
VGSHVPLNVS EENSDSNILD STLMYSQESL PRDNILSMEN DRLLREKRFH 1000
GIALLTKDNT LFKDNVSLMK TNKTYNHSTT NEKLHTESPT SIENSTTDLQ 1050
DAILKVNSEI QEVTALIHDG TLLGKNSTYL RLNHMLNRTT STKNKDIFHR 1100
KDEDPIPQDE ENTIMPFSKM LFLSESSNWF KKTNGNNSLN SEQEHSPKQL 1150
VYLMFKKYVK NQSFLSEKNK VTVEQDGFTK NIGLKDMAFP HNMSIFLTTL 1200
SNVHENGRHN QEKNIQEEIE KEALIEEKVV LPQVHEATGS KNFLKDILIL 1250
GTRQNISLYE VHVPVLQNIT SINNSTNTVQ IHMEHFFKRR KDKETNSEGL 1300
VNKTREMVKN YPSQKNITTQ RSKRALGQFR LSTQWLKTIN CSTQCIIKQI 1350
DHSKEMKKFI TKSSLSDSSV IKSTTQTNSS DSHIVKTSAF PPIDLKRSPF 1400
QNKFSHVQAS SYIYDFKTKS SRIQESNNFL KETKINNPSL AILPWNMFID 1450
QGKFTSPGKS NTNSVTYKKR ENIIFLKPTL PEESGKIELL PQVSIQEEEI 1500
LPTETSHGSP GHLNLMKEVF LQKIQGPTKW NKAKRHGESI KGKTESSKNT 1550
RSKLLNHHAW DYHYAAQIPK DMWKSKEKSP EIISIKQEDT ILSLRPHGNS 1600
HSIGANEKQN WPQRETTWVK QGQTQRTCSQ IPPVLKRHQR ELSAFQSEQE 1650
ATDYDDAITI ETIEDFDIYS EDIKQGPRSF QQKTRHYFIA AVERLWDYGM 1700
STSHVLRNRY QSDNVPQFKK VVFQEFTDGS FSQPLYRGEL NEHLGLLGPY 1750
IRAEVEDNIM VTFKNQASRP YSFYSSLISY KEDQRGEEPR RNFVKPNETK 1800
IYFWKVQHHM APTEDEFDCK AWAYFSDVDL ERDMHSGLIG PLLICHANTL 1850
NPAHGRQVSV QEFALLFTIF DETKSWYFTE NVKRNCKTPC NFQMEDPTLK 1900
ENYRFHAING YVMDTLPGLV MAQDQRIRWY LLSMGNNENI QSIHFSGHVF 1950
TVRKKEEYKM AVYNLYPGVF ETLEMIPSRA GIWRVECLIG EHLQAGMSTL 2000
FLVYSKQCQI PLGMASGSIR DFQITASGHY GQWAPNLARL HYSGSINAWS 2050
TKEPFSWIKV DLLAPMIVHG IKTQGARQKF SSLYISQFII MYSLDGKKWL 2100
SYQGNSTGTL MVFFGNVDSS GIKHNSFNPP IIARYIRLHP THSSIRSTLR 2150
MELMGCDLNS CSIPLGMESK VISDTQITAS SYFTNMFATW SPSQARLHLQ 2200
GRTNAWRPQV NDPKQWLQVD LQKTMKVTGI ITQGVKSLFT SMFVKEFLIS 2250
SSQDGHHWTQ ILYNGKVKVF QGNQDSSTPM MNSLDPPLLT RYLRIHPQIW 2300
EHQIALRLEI LGCEAQQQY 2319
Length:2,319
Mass (Da):266,196
Last modified:July 27, 2011 - v2
Checksum:i09CAC4CFBCCECA57
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti959 – 9591V → A in AAA37385. 1 Publication
Sequence conflicti988 – 9881M → I in AAA37385. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L05573 mRNA. Translation: AAA37385.1.
AL731844, AL808110 Genomic DNA. Translation: CAM15581.1.
AL808110, AL731844 Genomic DNA. Translation: CAM26492.1.
CH466576 Genomic DNA. Translation: EDL29229.1.
CCDSiCCDS30238.1.
PIRiA47004.
RefSeqiNP_032003.2. NM_007977.2.
UniGeneiMm.1805.

Genome annotation databases

EnsembliENSMUST00000033539; ENSMUSP00000033539; ENSMUSG00000031196.
GeneIDi14069.
KEGGimmu:14069.
UCSCiuc009tpt.3. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L05573 mRNA. Translation: AAA37385.1 .
AL731844 , AL808110 Genomic DNA. Translation: CAM15581.1 .
AL808110 , AL731844 Genomic DNA. Translation: CAM26492.1 .
CH466576 Genomic DNA. Translation: EDL29229.1 .
CCDSi CCDS30238.1.
PIRi A47004.
RefSeqi NP_032003.2. NM_007977.2.
UniGenei Mm.1805.

3D structure databases

ProteinModelPortali Q06194.
SMRi Q06194. Positions 20-744, 1678-2319.
ModBasei Search...

PTM databases

PhosphoSitei Q06194.

Proteomic databases

PaxDbi Q06194.
PRIDEi Q06194.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000033539 ; ENSMUSP00000033539 ; ENSMUSG00000031196 .
GeneIDi 14069.
KEGGi mmu:14069.
UCSCi uc009tpt.3. mouse.

Organism-specific databases

CTDi 2157.
MGIi MGI:88383. F8.

Phylogenomic databases

eggNOGi NOG151351.
GeneTreei ENSGT00740000114988.
HOGENOMi HOG000231686.
HOVERGENi HBG106657.
InParanoidi A2AN88.
KOi K03899.
OMAi WHVIGMG.
OrthoDBi EOG7ZWD1D.
TreeFami TF329807.

Miscellaneous databases

NextBioi 285060.
PROi Q06194.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q06194.
Bgeei Q06194.
CleanExi MM_F8.
Genevestigatori Q06194.

Family and domain databases

Gene3Di 2.60.120.260. 2 hits.
2.60.40.420. 6 hits.
InterProi IPR000421. Coagulation_fac_5/8-C_type_dom.
IPR011706. Cu-oxidase_2.
IPR011707. Cu-oxidase_3.
IPR002355. Cu_oxidase_Cu_BS.
IPR008972. Cupredoxin.
IPR024715. Factor_5/8.
IPR014707. Factor_8.
IPR008979. Galactose-bd-like.
[Graphical view ]
PANTHERi PTHR10127:SF50. PTHR10127:SF50. 1 hit.
Pfami PF07731. Cu-oxidase_2. 1 hit.
PF07732. Cu-oxidase_3. 2 hits.
PF00754. F5_F8_type_C. 2 hits.
[Graphical view ]
PIRSFi PIRSF000354. Factors_V_VIII. 1 hit.
SMARTi SM00231. FA58C. 2 hits.
[Graphical view ]
SUPFAMi SSF49503. SSF49503. 6 hits.
SSF49785. SSF49785. 2 hits.
PROSITEi PS01285. FA58C_1. 2 hits.
PS01286. FA58C_2. 2 hits.
PS50022. FA58C_3. 2 hits.
PS00079. MULTICOPPER_OXIDASE1. 3 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence of the murine factor VIII cDNA."
    Elder B., Lakich D., Gitschier J.
    Genomics 16:374-379(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6 X CBA.
    Tissue: Liver.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiFA8_MOUSE
AccessioniPrimary (citable) accession number: Q06194
Secondary accession number(s): A2AN88
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi