Q06190 (P2R3A_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit alpha

Alternative name(s):
PP2A subunit B isoform PR72/PR130
PP2A subunit B isoform R3 isoform
PP2A subunit B isoforms B''-PR72/PR130
PP2A subunit B isoforms B72/B130
Serine/threonine-protein phosphatase 2A 72/130 kDa regulatory subunit B

Gene names

Name:	PPP2R3A
Synonyms:	PPP2R3

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1150 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.
Subunit structure	PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules.
Tissue specificity	Expressed in heart, brain, placenta, lung, muscle and kidney.
Sequence similarities	Contains 2 EF-hand domains.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat
Ligand	Calcium Metal-binding
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	Wnt receptor signaling pathway involved in somitogenesis Inferred by curator PubMed 15687260. Source: BHF-UCL eye photoreceptor cell differentiation Inferred from genetic interaction PubMed 15687260. Source: BHF-UCL negative regulation of canonical Wnt receptor signaling pathway Inferred from genetic interaction PubMed 15687260. Source: BHF-UCL positive regulation of canonical Wnt receptor signaling pathway Inferred from direct assay PubMed 16567647. Source: BHF-UCL positive regulation of protein catabolic process Inferred from mutant phenotype PubMed 15687260. Source: BHF-UCL protein dephosphorylation Inferred from sequence or structural similarity PubMed 1849734. Source: UniProtKB regulation of cell motility involved in somitogenic axis elongation Inferred from genetic interaction PubMed 15687260. Source: BHF-UCL somatic muscle development Inferred from genetic interaction PubMed 15687260. Source: BHF-UCL somite development Inferred from sequence or structural similarity PubMed 16567647. Source: BHF-UCL
Cellular_component	protein phosphatase type 2A complex Inferred from direct assay PubMed 17055435. Source: UniProtKB
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro protein phosphatase type 2A regulator activity Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
NKD1	Q969G9	4	EBI-949204,EBI-1538217

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform PR130 (identifier: Q06190-1)

Also known as: 130 kDa;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform PR72 (identifier: Q06190-2)

Also known as: 72 kDa;

The sequence of this isoform differs from the canonical sequence as follows:
     1-621: Missing.
     622-665: MQILQETLTT...LIQQTPEVIK → MMIKETSLRR...KRLKSFQQTQ

Isoform 3 (identifier: Q06190-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-736: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1150	1150	Serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit alpha		PRO_0000071443
Regions
	Domain	758 – 793	36	EF-hand 1
	Domain	972 – 1007	36	EF-hand 2
	Calcium binding	985 – 996	12	Potential
	Compositional bias	670 – 695	26	Pro-rich
Natural variations
	Alternative sequence	1 – 736	736	Missing in isoform 3.		VSP_045203
	Alternative sequence	1 – 621	621	Missing in isoform PR72.		VSP_005107
	Alternative sequence	622 – 665	44	MQILQ…PEVIK → MMIKETSLRRDPDLRGELAF LARGCDFVLPSRFKKRLKSF QQTQ in isoform PR72.		VSP_005108
	Natural variant	67	1	D → G. Corresponds to variant rs9814557 [ dbSNP | Ensembl ].		VAR_051739
	Natural variant	67	1	D → N. Corresponds to variant rs57374999 [ dbSNP | Ensembl ].		VAR_061760
	Natural variant	108	1	N → S. Corresponds to variant rs36020282 [ dbSNP | Ensembl ].		VAR_051740
	Natural variant	171	1	A → S. Corresponds to variant rs6779903 [ dbSNP | Ensembl ].		VAR_051741
	Natural variant	481	1	P → A. Corresponds to variant rs34901937 [ dbSNP | Ensembl ].		VAR_051742
	Natural variant	642	1	S → G. Corresponds to variant rs17197552 [ dbSNP | Ensembl ].		VAR_051743
	Natural variant	695	1	P → L. Corresponds to variant rs9826032 [ dbSNP | Ensembl ].		VAR_051744
	Natural variant	745	1	D → N. Corresponds to variant rs16843645 [ dbSNP | Ensembl ].		VAR_022095

Sequences

	Sequence		Length	Mass (Da)
	Isoform PR130 (130 kDa) [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 97A31BA4206518A3	FASTA	1,150	130,278
	10 20 30 40 50 60 MAATYRLVVS TVNHYSSVVI DRRFEQAIHY CTGTCHTFTH GIDCIVVHHS VCADLLHIPV 70 80 90 100 110 120 SQFKDADLNS MFLPHENGLS SAEGDYPQQA FTGIPRVKRG STFQNTYNLK DIAGEAISFA 130 140 150 160 170 180 SGKIKEFSFE KLKNSNHAAY RKGRKVKSDS FNRRSVDLDL LCGHYNNDGN APSFGLLRSS 190 200 210 220 230 240 SVEEKPLSHR NSLDTNLTSM FLQNFSEEDL VTQILEKHKI DNFSSGTDIK MCLDILLKCS 250 260 270 280 290 300 EDLKKCTDII KQCIKKKSGS SISEGSGNDT ISSSETVYMN VMTRLASYLK KLPFEFMQSG 310 320 330 340 350 360 NNEALDLTEL ISNMPSLQLT PFSPVFGTEQ PPKYEDVVQL SASDSGRFQT IELQNDKPNS 370 380 390 400 410 420 RKMDTVQSIP NNSTNSLYNL EVNDPRTLKA VQVQSQSLTM NPLENVSSDD LMETLYIEEE 430 440 450 460 470 480 SDGKKALDKG QKTENGPSHE LLKVNEHRAE FPEHATHLKK CPTPMQNEIG KIFEKSFVNL 490 500 510 520 530 540 PKEDCKSKVS KFEEGDQRDF TNSSSQEEID KLLMDLESFS QKMETSLREP LAKGKNSNFL 550 560 570 580 590 600 NSHSQLTGQT LVDLEPKSKV SSPIEKVSPS CLTRIIETNG HKIEEEDRAL LLRILESIED 610 620 630 640 650 660 FAQELVECKS SRGSLSQEKE MMQILQETLT TSSQANLSVC RSPVGDKAKD TTSAVLIQQT 670 680 690 700 710 720 PEVIKIQNKP EKKPGTPLPP PATSPSSPRP LSPVPHVNNV VNAPLSINIP RFYFPEGLPD 730 740 750 760 770 780 TCSNHEQTLS RIETAFMDIE EQKADIYEMG KIAKVCGCPL YWKAPMFRAA GGEKTGFVTA 790 800 810 820 830 840 QSFIAMWRKL LNNHHDDASK FICLLAKPNC SSLEQEDFIP LLQDVVDTHP GLTFLKDAPE 850 860 870 880 890 900 FHSRYITTVI QRIFYTVNRS WSGKITSTEI RKSNFLQTLA LLEEEEDINQ ITDYFSYEHF 910 920 930 940 950 960 YVIYCKFWEL DTDHDLYISQ ADLSRYNDQA SSSRIIERIF SGAVTRGKTI QKEGRMSYAD 970 980 990 1000 1010 1020 FVWFLISEED KRNPTSIEYW FRCMDVDGDG VLSMYELEYF YEEQCERMEA MGIEPLPFHD 1030 1040 1050 1060 1070 1080 LLCQMLDLVK PAVDGKITLR DLKRCRMAHI FYDTFFNLEK YLDHEQRDPF AVQKDVENDG 1090 1100 1110 1120 1130 1140 PEPSDWDRFA AEEYETLVAE ESAQAQFQEG FEDYETDEPA SPSEFGNKSN KILSASLPEK 1150 CGKLQSVDEE
	Isoform PR72 (72 kDa) [UniParc]. Checksum: 3782D0D800A5F6D4	FASTA	529	61,097
	10 20 30 40 50 60 MMIKETSLRR DPDLRGELAF LARGCDFVLP SRFKKRLKSF QQTQIQNKPE KKPGTPLPPP 70 80 90 100 110 120 ATSPSSPRPL SPVPHVNNVV NAPLSINIPR FYFPEGLPDT CSNHEQTLSR IETAFMDIEE 130 140 150 160 170 180 QKADIYEMGK IAKVCGCPLY WKAPMFRAAG GEKTGFVTAQ SFIAMWRKLL NNHHDDASKF 190 200 210 220 230 240 ICLLAKPNCS SLEQEDFIPL LQDVVDTHPG LTFLKDAPEF HSRYITTVIQ RIFYTVNRSW 250 260 270 280 290 300 SGKITSTEIR KSNFLQTLAL LEEEEDINQI TDYFSYEHFY VIYCKFWELD TDHDLYISQA 310 320 330 340 350 360 DLSRYNDQAS SSRIIERIFS GAVTRGKTIQ KEGRMSYADF VWFLISEEDK RNPTSIEYWF 370 380 390 400 410 420 RCMDVDGDGV LSMYELEYFY EEQCERMEAM GIEPLPFHDL LCQMLDLVKP AVDGKITLRD 430 440 450 460 470 480 LKRCRMAHIF YDTFFNLEKY LDHEQRDPFA VQKDVENDGP EPSDWDRFAA EEYETLVAEE 490 500 510 520 SAQAQFQEGF EDYETDEPAS PSEFGNKSNK ILSASLPEKC GKLQSVDEE
	Isoform 3 [UniParc]. Checksum: 3F0B982FF10C8AD9	FASTA	414	48,178
	10 20 30 40 50 60 MDIEEQKADI YEMGKIAKVC GCPLYWKAPM FRAAGGEKTG FVTAQSFIAM WRKLLNNHHD 70 80 90 100 110 120 DASKFICLLA KPNCSSLEQE DFIPLLQDVV DTHPGLTFLK DAPEFHSRYI TTVIQRIFYT 130 140 150 160 170 180 VNRSWSGKIT STEIRKSNFL QTLALLEEEE DINQITDYFS YEHFYVIYCK FWELDTDHDL 190 200 210 220 230 240 YISQADLSRY NDQASSSRII ERIFSGAVTR GKTIQKEGRM SYADFVWFLI SEEDKRNPTS 250 260 270 280 290 300 IEYWFRCMDV DGDGVLSMYE LEYFYEEQCE RMEAMGIEPL PFHDLLCQML DLVKPAVDGK 310 320 330 340 350 360 ITLRDLKRCR MAHIFYDTFF NLEKYLDHEQ RDPFAVQKDV ENDGPEPSDW DRFAAEEYET 370 380 390 400 410 LVAEESAQAQ FQEGFEDYET DEPASPSEFG NKSNKILSAS LPEKCGKLQS VDEE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure and expression of a 72-kDa regulatory subunit of protein phosphatase 2A. Evidence for different size forms produced by alternative splicing." Hendrix P., Mayer-Jaekel R.E., Cron P., Goris J., Hofsteenge J., Merlevede W., Hemmings B.A. J. Biol. Chem. 268:15267-15276(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS PR72 AND PR130), PARTIAL PROTEIN SEQUENCE, ALTERNATIVE SPLICING. Tissue: Fetal brain.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS PR72; PR130 AND 3). Tissue: Lung, Tongue and Trachea.
[3]	"The DNA sequence, annotation and analysis of human chromosome 3." Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J. , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A. Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	The European IMAGE consortium Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 768-1150.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L07590 mRNA. Translation: AAB02613.1. L12146 mRNA. Translation: AAB02614.1. AK293012 mRNA. Translation: BAF85701.1. AK298059 mRNA. Translation: BAG60353.1. AK316258 mRNA. Translation: BAH14629.1. AC072039 Genomic DNA. No translation available. AC092991 Genomic DNA. No translation available. CH471052 Genomic DNA. Translation: EAW79122.1. CH471052 Genomic DNA. Translation: EAW79123.1. AL389975 mRNA. Translation: CAB97532.1.
IPI	IPI00029144. IPI00218930. IPI00944998.
PIR	A47114. B47114.
RefSeq	NP_001177376.1. NM_001190447.1. NP_002709.2. NM_002718.4. NP_871626.1. NM_181897.2.
UniGene	Hs.518155.
3D structure databases
ProteinModelPortal	Q06190.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-29397N.
IntAct	Q06190. 5 interactions.
MINT	MINT-2861926.
STRING	9606.ENSP00000264977.
PTM databases
PhosphoSite	Q06190.
Polymorphism databases
DMDM	543720.
Proteomic databases
PaxDb	Q06190.
PRIDE	Q06190.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000264977; ENSP00000264977; ENSG00000073711. ENST00000334546; ENSP00000334748; ENSG00000073711. ENST00000490467; ENSP00000419344; ENSG00000073711.
GeneID	5523.
KEGG	hsa:5523.
UCSC	uc003eqv.2. human. uc003eqw.2. human.
Organism-specific databases
CTD	5523.
GeneCards	GC03P135684.
HGNC	HGNC:9307. PPP2R3A.
HPA	HPA035829. HPA035830.
MIM	604944. gene.
neXtProt	NX_Q06190.
PharmGKB	PA35523.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG271066.
HOGENOM	HOG000013216.
HOVERGEN	HBG000013.
InParanoid	Q06190.
KO	K11583.
OMA	FQTIELQ.
OrthoDB	EOG4PC9RJ.
PhylomeDB	Q06190.
Gene expression databases
ArrayExpress	Q06190.
Bgee	Q06190.
CleanEx	HS_PPP2R3A.
Genevestigator	Q06190.
GermOnline	ENSG00000073711. Homo sapiens.
Family and domain databases
Gene3D	1.10.238.10. 1 hit.
InterPro	IPR011992. EF-hand-like_dom. IPR018247. EF_Hand_1_Ca_BS. IPR002048. EF_hand_dom. [Graphical view]
Pfam	PF13499. EF_hand_5. 1 hit. [Graphical view]
PROSITE	PS00018. EF_HAND_1. 1 hit. PS50222. EF_HAND_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	5523.
NextBio	21376.
SOURCE	Search...

Entry information

Entry name

P2R3A_HUMAN

Accession

Primary (citable) accession number: Q06190
Secondary accession number(s): A8KAE7 , B4DNU1, B7ZAE3, Q06189, Q9NPQ5

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	May 1, 2013
This is version 123 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.