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Q06186 (HBEGF_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 118. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Proheparin-binding EGF-like growth factor

Cleaved into the following chain:

  1. Heparin-binding EGF-like growth factor
    Short name=HB-EGF
    Short name=HBEGF
Gene names
Name:Hbegf
Synonyms:Dtr, Hegfl
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length208 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Growth factor that mediates its effects via EGFR, ERBB2 and ERBB4. Required for normal cardiac valve formation and normal heart function. Promotes smooth muscle cell proliferation. May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts, but not endothelial cells. It is able to bind EGF receptor/EGFR with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor.

Subunit structure

Interacts with FBLN1 By similarity. Interacts with EGFR and ERBB4.

Subcellular location

Heparin-binding EGF-like growth factor: Secretedextracellular space. Note: Mature HB-EGF is released into the extracellular space and probably binds to a receptor.

Proheparin-binding EGF-like growth factor: Cell membrane; Single-pass type I membrane protein.

Tissue specificity

Most abundant in kidney, skeletal muscle, lung, spleen, brain and heart.

Post-translational modification

O-glycosylated By similarity.

Sequence similarities

Contains 1 EGF-like domain.

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
Secreted
   DomainEGF-like domain
Signal
Transmembrane
Transmembrane helix
   LigandHeparin-binding
   Molecular functionGrowth factor
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processangiogenesis

Non-traceable author statement PubMed 15289334. Source: UniProtKB

blastocyst growth

Traceable author statement PubMed 10101133. Source: UniProtKB

cell migration

Inferred from mutant phenotype PubMed 15923649. Source: UniProtKB

epidermal growth factor receptor signaling pathway

Inferred from direct assay PubMed 12621152. Source: MGI

negative regulation of elastin biosynthetic process

Inferred from electronic annotation. Source: Ensembl

positive regulation of cell growth

Inferred from electronic annotation. Source: Ensembl

positive regulation of keratinocyte migration

Inferred from mutant phenotype PubMed 15923649. Source: UniProtKB

positive regulation of protein kinase B signaling

Inferred from electronic annotation. Source: Ensembl

positive regulation of smooth muscle cell proliferation

Inferred from direct assay PubMed 1840698. Source: UniProtKB

positive regulation of wound healing

Inferred from electronic annotation. Source: Ensembl

regulation of heart contraction

Inferred from mutant phenotype PubMed 12621152. Source: MGI

wound healing, spreading of epidermal cells

Inferred from mutant phenotype PubMed 15923649. Source: UniProtKB

   Cellular_componentcell surface

Inferred from electronic annotation. Source: Ensembl

extracellular region

Traceable author statement. Source: Reactome

extracellular space

Inferred from direct assay PubMed 9857183. Source: BHF-UCL

integral component of plasma membrane

Inferred from direct assay PubMed 9857183. Source: BHF-UCL

   Molecular_functionepidermal growth factor receptor binding

Inferred from direct assay PubMed 1840698. Source: UniProtKB

growth factor activity

Inferred from direct assay PubMed 1840698. Source: UniProtKB

heparin binding

Inferred from direct assay PubMed 1840698. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Potential
Chain24 – 208185Proheparin-binding EGF-like growth factor
PRO_0000302804
Propeptide24 – 6239 By similarity
PRO_0000007614
Chain63 – 14886Heparin-binding EGF-like growth factor
PRO_0000007615
Propeptide149 – 20860C-terminal Potential
PRO_0000007616

Regions

Topological domain24 – 160137Extracellular Potential
Transmembrane161 – 18424Helical; Potential
Topological domain185 – 20824Cytoplasmic Potential
Domain104 – 14441EGF-like

Amino acid modifications

Glycosylation851O-linked (GalNAc...) By similarity
Disulfide bond108 ↔ 121 By similarity
Disulfide bond116 ↔ 132 By similarity
Disulfide bond134 ↔ 143 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q06186 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 7086934E23D25F8E

FASTA20822,808
        10         20         30         40         50         60 
MKLLPSVMLK LFLAAVLSAL VTGESLERLR RGLAAATSNP DPPTGSTNQL LPTGGDRAQG 

        70         80         90        100        110        120 
VQDLEGTDLN LFKVAFSSKP QGLATPSKER NGKKKKKGKG LGKKRDPCLR KYKDYCIHGE 

       130        140        150        160        170        180 
CRYLQEFRTP SCKCLPGYHG HRCHGLTLPV ENPLYTYDHT TVLAVVAVVL SSVCLLVIVG 

       190        200 
LLMFRYHRRG GYDLESEEKV KLGVASSH 

« Hide

References

[1]"Heparin-binding EGF-like growth factor: characterization of rat and mouse cDNA clones, protein domain conservation across species, and transcript expression in tissues."
Abraham J.A., Damm D., Bajardi A., Miller J., Klagsbrun M., Ezekowitz R.A.B.
Biochem. Biophys. Res. Commun. 190:125-133(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Macrophage.
[2]"Characterization of the gene encoding murine heparin-binding epidermal growth factor-like growth factor."
Harding P.A., Brigstock D.R., Shen L., Crissman-Combs M.A., Besner G.E.
Gene 169:291-292(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 129/SvJ.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L36027 expand/collapse EMBL AC list , L36024, L36025, L36026 Genomic DNA. Translation: AAC42069.1.
L07264 mRNA. Translation: AAA37542.1.
U39192 expand/collapse EMBL AC list , U39189, U39190, U39191 Genomic DNA. Translation: AAC52617.1.
CCDSCCDS29153.1.
PIRJC1410.
RefSeqNP_034545.1. NM_010415.2.
UniGeneMm.289681.

3D structure databases

ProteinModelPortalQ06186.
SMRQ06186. Positions 107-147.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000025363.

PTM databases

PhosphoSiteQ06186.

Proteomic databases

PRIDEQ06186.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000025363; ENSMUSP00000025363; ENSMUSG00000024486.
GeneID15200.
KEGGmmu:15200.
UCSCuc008enl.2. mouse.

Organism-specific databases

CTD1839.
MGIMGI:96070. Hbegf.

Phylogenomic databases

eggNOGNOG45526.
HOGENOMHOG000026782.
HOVERGENHBG053952.
InParanoidQ06186.
KOK08523.
OMAYSYDHTT.
OrthoDBEOG7VQJGP.
PhylomeDBQ06186.
TreeFamTF332773.

Enzyme and pathway databases

ReactomeREACT_188257. Signal Transduction.

Gene expression databases

ArrayExpressQ06186.
BgeeQ06186.
CleanExMM_HBEGF.
GenevestigatorQ06186.

Family and domain databases

InterProIPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR015497. EGF_rcpt_ligand.
[Graphical view]
PANTHERPTHR10740. PTHR10740. 1 hit.
PfamPF00008. EGF. 1 hit.
[Graphical view]
SMARTSM00181. EGF. 1 hit.
[Graphical view]
PROSITEPS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio287735.
PMAP-CutDBQ06186.
PROQ06186.
SOURCESearch...

Entry information

Entry nameHBEGF_MOUSE
AccessionPrimary (citable) accession number: Q06186
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: July 9, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot