Q06185 (ATP5I_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP synthase subunit e, mitochondrial
Short name=ATPase subunit e

Gene names

Name:	Atp5i
Synonyms:	Atp5k, Lfm-1, Lfm1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	71 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core, and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F0 domain. Minor subunit located with subunit a in the membrane.
Subunit structure	F-type ATPases have 2 components, CF1 - the catalytic core - and CF0 - the membrane proton channel. CF0 seems to have nine subunits: a, b, c, d, e, f, g, F6 and 8 (or A6L). Component of an ATP synthase complex composed of ATP5F1, ATP5G1, ATP5E, ATP5H, ATP5I, ATP5J, ATP5J2, MT-ATP6, MT-ATP8, ATP5A1, ATP5B, ATP5D, ATP5C1, ATP5O, ATP5L, USMG5 and MP68 By similarity.
Subcellular location	Mitochondrion. Mitochondrion inner membrane.
Tissue specificity	Mammary gland, liver, kidney, heart, spleen, brain and lung.
Sequence similarities	Belongs to the ATPase e subunit family.

Ontologies

Keywords
Biological process	ATP synthesis Hydrogen ion transport Ion transport Transport
Cellular component	CF(0) Membrane Mitochondrion Mitochondrion inner membrane
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	ATP synthesis coupled proton transport Inferred from electronic annotation. Source: InterPro
Cellular_component	mitochondrial proton-transporting ATP synthase complex Inferred from sequence or structural similarity. Source: UniProtKB mitochondrial proton-transporting ATP synthase complex, coupling factor F(o) Inferred from electronic annotation. Source: Compara
Molecular_function	ATPase activity Inferred from electronic annotation. Source: Compara hydrogen ion transmembrane transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 71	70	ATP synthase subunit e, mitochondrial		PRO_0000071685
Amino acid modifications
	Modified residue	32	1	Phosphotyrosine Ref.5
Experimental info
	Sequence conflict	13	1	F → S in AAC52713. Ref.2
	Sequence conflict	48	1	K → R in AAC52713. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q06185 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 0379C3E14C098F12	FASTA	71	8,236
	10 20 30 40 50 60 MVPPVQVSPL IKFGRYSALI IGMAYGAKRY SYLKPRAEEE RRIAAEEKKR LDELKRIERE 70 LAEAQDDSIL K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"F1F0-ATPase subunit e gene isolated in a screen for diet regulated genes." Elliott T.S., Swartz D.A., Paisley E.A., Mangian H.J., Visek W.J., Kaput J. Biochem. Biophys. Res. Commun. 190:167-174(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c. Tissue: Mammary gland.
[2]	"The e subunit gene of murine F1F0-ATP synthase. Genomic sequence, chromosomal mapping, and diet regulation." Swartz D.A., Park E.I., Visek W.J., Kaput J. J. Biol. Chem. 271:20942-20948(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: BALB/cJ.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Mammary gland.
[4]	Lubec G., Kang S.U. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-12; 16-28 AND 60-71, MASS SPECTROMETRY. Strain: C57BL/6. Tissue: Brain.
[5]	"Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain." Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P. J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-32, MASS SPECTROMETRY. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	S52977 mRNA. Translation: AAB24947.1. U59283 Genomic DNA. Translation: AAC52713.1. BC028438 mRNA. Translation: AAH28438.1.
IPI	IPI00111770.
PIR	JC1412.
RefSeq	NP_031533.2. NM_007507.2.
UniGene	Mm.136093.
3D structure databases
ProteinModelPortal	Q06185.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-1840518.
STRING	10090.ENSMUSP00000051222.
PTM databases
PhosphoSite	Q06185.
Proteomic databases
PaxDb	Q06185.
PRIDE	Q06185.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000049628; ENSMUSP00000051222; ENSMUSG00000050856.
GeneID	11958.
KEGG	mmu:11958.
UCSC	uc008yoa.1. mouse.
Organism-specific databases
CTD	11958.
MGI	MGI:106636. Atp5k.
Phylogenomic databases
eggNOG	NOG81326.
GeneTree	ENSGT00390000005102.
HOGENOM	HOG000231826.
HOVERGEN	HBG050613.
InParanoid	Q06185.
KO	K02129.
OMA	EQERIYK.
OrthoDB	EOG45758D.
Gene expression databases
ArrayExpress	Q06185.
Bgee	Q06185.
Genevestigator	Q06185.
Family and domain databases
InterPro	IPR008386. ATPase_F0-cplx_esu_mt. [Graphical view]
Pfam	PF05680. ATP-synt_E. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	280081.
SOURCE	Search...

Entry information

Entry name

ATP5I_MOUSE

Accession

Primary (citable) accession number: Q06185
Secondary accession number(s): P70342

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	January 23, 2007
Last modified:	May 29, 2013
This is version 99 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program