Q06180 (PTN2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tyrosine-protein phosphatase non-receptor type 2
Short name=Protein-tyrosine phosphatase PTP-2
EC=3.1.3.48

Alternative name(s):
MPTP

Gene names

Name:	Ptpn2
Synonyms:	Ptpt

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	406 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.
Subunit structure	Interacts with RMDN3. Interacts with TMED9 By similarity.
Subcellular location	Cytoplasm. Endoplasmic reticulum By similarity. Nucleus By similarity.
Tissue specificity	Found in all the examined tissues. The highest expression levels were found in ovaries, testes, thymus and kidneys. Also present in spleen, mouse, liver, heart and brain.
Sequence similarities	Belongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily. Contains 1 tyrosine-protein phosphatase domain.

Ontologies

Keywords
Cellular component	Cytoplasm Endoplasmic reticulum Nucleus
Coding sequence diversity	Alternative splicing
Molecular function	Hydrolase Protein phosphatase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	insulin receptor signaling pathway Inferred from mutant phenotype PubMed 15632081. Source: MGI peptidyl-tyrosine dephosphorylation Inferred from electronic annotation. Source: GOC
Cellular_component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	non-membrane spanning protein tyrosine phosphatase activity Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform PTPB (identifier: Q06180-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform PTPA (identifier: Q06180-2)

The sequence of this isoform differs from the canonical sequence as follows:
     377-406: WLYWQPILTKMGFVSVILVGALVGWTLLFH → PRLTDT

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 406	406	Tyrosine-protein phosphatase non-receptor type 2		PRO_0000094753
Regions
	Domain	5 – 275	271	Tyrosine-protein phosphatase
	Region	216 – 222	7	Substrate binding By similarity
Sites
	Active site	216	1	Phosphocysteine intermediate By similarity
	Binding site	182	1	Substrate By similarity
	Binding site	260	1	Substrate By similarity
Amino acid modifications
	Modified residue	298	1	Phosphoserine By similarity
	Modified residue	304	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	377 – 406	30	WLYWQ…TLLFH → PRLTDT in isoform PTPA.		VSP_012367

Sequences

	Sequence		Length	Mass (Da)
	Isoform PTPB [UniParc]. Last modified January 4, 2005. Version 2. Checksum: DFB881DF3C800DC3	FASTA	406	47,360
	10 20 30 40 50 60 MSATIEREFE ELDAQCRWQP LYLEIRNESH DYPHRVAKFP ENRNRNRYRD VSPYDHSRVK 70 80 90 100 110 120 LQSTENDYIN ASLVDIEEAQ RSYILTQGPL PNTCCHFWLM VWQQKTKAVV MLNRTVEKES 130 140 150 160 170 180 VKCAQYWPTD DREMVFKETG FSVKLLSEDV KSYYTVHLLQ LENINTGETR TISHFHYTTW 190 200 210 220 230 240 PDFGVPESPA SFLNFLFKVR ESGCLTPDHG PAVIHCSAGI GRSGTFSLVD TCLVLMEKGE 250 260 270 280 290 300 DVNVKQLLLN MRKYRMGLIQ TPDQLRFSYM AIIEGAKYTK GDSNIQKRWK ELSKEDLSPI 310 320 330 340 350 360 CDHSQNRVMV EKYNGKRIGS EDEKLTGLPS KVQDTVEESS ESILRKRIRE DRKATTAQKV 370 380 390 400 QQMKQRLNET ERKRKRWLYW QPILTKMGFV SVILVGALVG WTLLFH
	Isoform PTPA [UniParc]. Checksum: BFE174ABC0963929	FASTA	382	44,573
	10 20 30 40 50 60 MSATIEREFE ELDAQCRWQP LYLEIRNESH DYPHRVAKFP ENRNRNRYRD VSPYDHSRVK 70 80 90 100 110 120 LQSTENDYIN ASLVDIEEAQ RSYILTQGPL PNTCCHFWLM VWQQKTKAVV MLNRTVEKES 130 140 150 160 170 180 VKCAQYWPTD DREMVFKETG FSVKLLSEDV KSYYTVHLLQ LENINTGETR TISHFHYTTW 190 200 210 220 230 240 PDFGVPESPA SFLNFLFKVR ESGCLTPDHG PAVIHCSAGI GRSGTFSLVD TCLVLMEKGE 250 260 270 280 290 300 DVNVKQLLLN MRKYRMGLIQ TPDQLRFSYM AIIEGAKYTK GDSNIQKRWK ELSKEDLSPI 310 320 330 340 350 360 CDHSQNRVMV EKYNGKRIGS EDEKLTGLPS KVQDTVEESS ESILRKRIRE DRKATTAQKV 370 380 QQMKQRLNET ERKRKRPRLT DT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of a mouse cDNA encoding a cytoplasmic protein-tyrosine-phosphatase." Mosinger B. Jr., Tillmann U., Westphal H., Tremblay M.L. Proc. Natl. Acad. Sci. U.S.A. 89:499-503(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM PTPA).
[2]	"Molecular cloning, nucleotide sequence and expression of a cDNA encoding an intracellular protein tyrosine phosphatase, PTPase-2, from mouse testis and T-cells." Miyasaka H., Li S.S.-L. Mol. Cell. Biochem. 118:91-98(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM PTPA). Strain: C57BL/6N. Tissue: T-cell and Testis.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM PTPB).
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM PTPB). Strain: FVB/N. Tissue: Mammary gland.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M81477 mRNA. Translation: AAA37446.1. S52655 mRNA. Translation: AAB25035.2. BC008269 mRNA. Translation: AAH08269.1. AK076072 mRNA. Translation: BAC36163.1. AK132013 mRNA. Translation: BAE20939.1.
IPI	IPI00515708. IPI00623994.
PIR	A38191.
RefSeq	NP_001120649.1. NM_001127177.1. NP_033003.1. NM_008977.3.
UniGene	Mm.260433.
3D structure databases
ProteinModelPortal	Q06180.
SMR	Q06180. Positions 5-277.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-5170362.
PTM databases
PhosphoSite	Q06180.
Proteomic databases
PaxDb	Q06180.
PRIDE	Q06180.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000025420; ENSMUSP00000025420; ENSMUSG00000024539. ENSMUST00000122412; ENSMUSP00000112675; ENSMUSG00000024539.
GeneID	19255.
KEGG	mmu:19255.
UCSC	uc008fmu.2. mouse. uc008fmv.2. mouse.
Organism-specific databases
CTD	5771.
MGI	MGI:97806. Ptpn2.
Phylogenomic databases
eggNOG	COG5599.
GeneTree	ENSGT00700000104092.
HOGENOM	HOG000273908.
HOVERGEN	HBG008321.
InParanoid	Q06180.
KO	K01104.
OMA	NTAQKVQ.
OrthoDB	EOG461447.
Gene expression databases
ArrayExpress	Q06180.
Bgee	Q06180.
CleanEx	MM_PTPN2.
Genevestigator	Q06180.
GermOnline	ENSMUSG00000024539. Mus musculus.
Family and domain databases
InterPro	IPR012265. Ptpn1/Ptpn2. IPR000387. Tyr/Dual-sp_Pase. IPR016130. Tyr_Pase_AS. IPR000242. Tyr_Pase_rcpt/non-rcpt. [Graphical view]
PANTHER	PTHR19134:SF57. PTHR19134:SF57. 1 hit.
Pfam	PF00102. Y_phosphatase. 1 hit. [Graphical view]
PIRSF	PIRSF000926. Tyr-Ptase_nr1. 1 hit.
PRINTS	PR00700. PRTYPHPHTASE.
SMART	SM00194. PTPc. 1 hit. [Graphical view]
PROSITE	PS00383. TYR_PHOSPHATASE_1. 1 hit. PS50056. TYR_PHOSPHATASE_2. 1 hit. PS50055. TYR_PHOSPHATASE_PTP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	296100.
SOURCE	Search...

Entry information

Entry name

PTN2_MOUSE

Accession

Primary (citable) accession number: Q06180
Secondary accession number(s): Q3V259, Q922E7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	January 4, 2005
Last modified:	May 29, 2013
This is version 117 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program