Reviewed, UniProtKB/Swiss-Prot Q06178 (NMA1_YEAST)

Names and origin

Protein names

Recommended name:
    Nicotinamide-nucleotide adenylyltransferase 1
    EC=2.7.7.1
Alternative name(s):
    NAD(+) pyrophosphorylase 1
    NAD(+) diphosphorylase 1
    NMN adenylyltransferase 1

Gene names

Name:	NMA1
Ordered Locus Names:	YLR328W
ORF Names:	L8543.16

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	401 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + nicotinamide ribonucleotide = diphosphate + NAD+.
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from nicotinamide ribonucleotide: step 1/1.
Subunit structure	Homotetramer.
Miscellaneous	Present with 5130 molecules/cell in log phase SD medium. Ref.3
Sequence similarities	Belongs to the eukaryotic NMN adenylyltransferase family.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Nucleotidyltransferase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from direct assay. Source: SGD nucleus Inferred from direct assay. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW identical protein binding Inferred from physical interaction. Source: IntAct nicotinamide-nucleotide adenylyltransferase activity Ref.2 Inferred from direct assay. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		1	EBI-11803,EBI-11803
NMA2	P53204	1	EBI-11803,EBI-23073
NNF1	P47149	1	EBI-11803,EBI-12098
SPF1	P39986	1	EBI-11803,EBI-3128
SRP1	Q02821	1	EBI-11803,EBI-1797
TEM1	P38987	1	EBI-11803,EBI-19113

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 401	401	Nicotinamide-nucleotide adenylyltransferase 1		PRO_0000135018
Regions
	Compositional bias	61 – 67	7	Poly-His
Amino acid modifications
	Modified residue	44	1	Phosphoserine Ref.5
	Modified residue	91	1	Phosphoserine Ref.4
	Modified residue	95	1	Phosphoserine Ref.5 Ref.4
	Modified residue	96	1	Phosphoserine Ref.5 Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q06178-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: A176964E56A30DD8	FASTA	401	45,859
	10 20 30 40 50 60 MDPTRAPDFK PPSADEELIP PPDPESKIPK SIPIIPYVLA DANSSIDAPF NIKRKKKHPK 70 80 90 100 110 120 HHHHHHHSRK EGNDKKHQHI PLNQDDFQPL SAEVSSEDDD ADFRSKERYG SDSTTESETR 130 140 150 160 170 180 GVQKYQIADL EEVPHGIVRQ ARTLEDYEFP SHRLSKKLLD PNKLPLVIVA CGSFSPITYL 190 200 210 220 230 240 HLRMFEMALD AISEQTRFEV IGGYYSPVSD NYQKQGLAPS YHRVRMCELA CERTSSWLMV 250 260 270 280 290 300 DAWESLQPSY TRTAKVLDHF NHEINIKRGG VATVTGEKIG VKIMLLAGGD LIESMGEPNV 310 320 330 340 350 360 WADADLHHIL GNYGCLIVER TGSDVRSFLL SHDIMYEHRR NILIIKQLIY NDISSTKVRL 370 380 390 400 FIRRAMSVQY LLPNSVIRYI QEHRLYVDQT EPVKQVLGNK E

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D. Nature 387:87-90(1997) [PubMed: 9169871] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Identification and characterization of YLR328W, the Saccharomyces cerevisiae structural gene encoding NMN adenylyltransferase. Expression and characterization of the recombinant enzyme." Emanuelli M., Carnevali F., Lorenzi M., Raffaelli N., Amici A., Ruggieri S., Magni G. FEBS Lett. 455:13-17(1999) [PubMed: 10428462] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE, CHARACTERIZATION.
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[4]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-91; SER-95 AND SER-96, MASS SPECTROMETRY.
[5]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-44; SER-95 AND SER-96, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U20618 Genomic DNA. Translation: AAB64524.1.
PIR	S53405.
RefSeq	NP_013432.1.
3D structure databases
HSSP	HSSP built from PDB template 1KR2 based on UniProtKB Q9HAN9.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:1228N.
IntAct	Q06178. 13 interactions.
Genome annotation databases
Ensembl	YLR328W. Saccharomyces cerevisiae. [Contig view]
GeneID	851039.
GenomeReviews	Gene locus YLR328W in contig Y13138_GR.
KEGG	sce:YLR328W.
NMPDR	fig|4932.3.peg.4452.
Organism-specific databases
CYGD	YLR328w.
SGD	S000004320. NMA1.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	Q06178.
OMA	Q06178. EPNVWAD.
Enzyme and pathway databases
BioCyc	MetaCyc:YLR328W-MON.
BRENDA	2.7.7.1. 250.
Gene expression databases
GermOnline	YLR328W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR004820. Cytidylyltransf. IPR005248. NAMN_adtrnsfrase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR12039. NAMN_adtrnsfrase. 1 hit.
Pfam	PF01467. CTP_transf_2. 1 hit. [Graphical view]
TIGRFAMs	TIGR00482. NAMN_adtrnsfrase. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	967639.

Entry information

Entry name

NMA1_YEAST

Accession

Primary (citable) accession number: Q06178

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)