Chromatin structure-remodeling complex subunit SFH1 - Saccharomyces cerevisiae (Baker's yeast)

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Reviewed, UniProtKB/Swiss-Prot Q06168 (SFH1_YEAST)

Last modified June 16, 2009. Version 68. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Chromatin structure-remodeling complex subunit SFH1
Alternative name(s):
    RSC complex subunit SFH1
    SNF5 homolog 1

Gene names

Name:	SFH1
Ordered Locus Names:	YLR321C
ORF Names:	L8543.4

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length	426 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Component of the chromatin structure-remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. This subunit is essential for mitotic growth and required for cell cycle progression. Ref.2 Ref.3 Ref.4 Ref.5 Ref.7 Ref.8 Ref.9
Subunit structure	Interacts directly with STH1. Component of the two forms of the RSC complex composed of at least either RSC1 or RSC2, and ARP7, ARP9, LDB7, NPL6, RSC3, RSC30, RSC4, RSC58, RSC6, RSC8, RSC9, SFH1, STH1, HTL1 and probably RTT102. The complexes interact with histone and histone variant components of centromeric chromatin. Ref.3 Ref.9 Ref.6
Subcellular location	Nucleus. Note: Localizes to centromeric and flanking chromatin. Association with these loci is dependent on STH1. Ref.9
Post-translational modification	Phosphorylated in the G1 phase. Ref.3 Ref.11
Miscellaneous	Present with 3940 molecules/cell in log phase SD medium. Ref.10
Sequence similarities	Belongs to the SNF5 family.

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Ontologies

Keywords
Biological process	Cell cycle Transcription Transcription regulation
Cellular component	Nucleus
Molecular function	Chromatin regulator
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	ATP-dependent chromatin remodeling Ref.7 Inferred from direct assay. Source: UniProtKB G2/M transition of mitotic cell cycle Ref.3 Inferred from mutant phenotype. Source: UniProtKB RNA elongation from RNA polymerase II promoter Inferred from direct assay. Source: SGD chromatin remodeling at centromere Ref.9 Inferred from mutant phenotype. Source: SGD chromosome segregation Ref.9 Inferred from genetic interaction. Source: SGD double-strand break repair Inferred from mutant phenotype. Source: SGD nucleosome disassembly Inferred from direct assay. Source: SGD regulation of DNA replication during S phase Inferred from genetic interaction. Source: SGD regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	RSC complex Ref.3 Ref.6 Ref.7 Inferred from direct assay. Source: UniProtKB nuclear chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	protein binding Ref.3 Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct	Notes
	Q06137	1	EBI-35813,EBI-33827

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 426

426

Chromatin structure-remodeling complex subunit SFH1

PRO_0000205962

Regions

Region

201 – 242

Interaction with STH1

Amino acid modifications

Modified residue

Phosphoserine Ref.11

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Sequences

Sequence

Length

Mass (Da)

Tools

Q06168-1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: C8D6F151249E8479
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FASTA

426

48,777

        10         20         30         40         50         60 
MSHQNQLIPQ AYISNFHNRL TNEDDGIPIF TMAQQTRQHK RAKVVNYAEY DNDLFDEFNM 

        70         80         90        100        110        120 
NGSNFNNADT HYKDNAVSHE NTPALTNGVT MDGSEYNVLE NMNGADSIIS NNKYDAGSNM 

       130        140        150        160        170        180 
VVESLSGLNS NNNASNGPSN KAQAQDIGNA VLPDLQDQHH NPFNILRYPK IRDTFINGKV 

       190        200        210        220        230        240 
VSPYRLNTDQ ETKANANSGE AIMIPITLDI EHMGHTIKDQ FLWNYNDDSI SPEEFASIYC 

       250        260        270        280        290        300 
KDLDMTSATL QTQIANIIKE QLKDLENIAA TEIMSDLHVI INLTCNLQDR FFEDNFQWNL 

       310        320        330        340        350        360 
NDKSLTPERF ATSIVQDLGL TREFIPLISQ SLHETILKIK KDWVDGHLIQ DHVPNDAAFG 

       370        380        390        400        410        420 
YLSGIRLDID ELGSNWCPRV EILTKEEIQK REIEKERNLR RLKRETDRLS RRGRRRLDDL 


ETTMRM

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. Hoheisel J.D. Nature 387:87-90(1997) [PubMed: 9169871] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"RSC, an essential, abundant chromatin-remodeling complex." Cairns B.R., Lorch Y., Li Y., Zhang M., Lacomis L., Erdjument-Bromage H., Tempst P., Du J., Laurent B.C., Kornberg R.D. Cell 87:1249-1260(1996) [PubMed: 8980231] [Abstract] Cited for: FUNCTION OF THE RSC COMPLEX.
[3]	"Sfh1p, a component of a novel chromatin-remodeling complex, is required for cell cycle progression." Cao Y., Cairns B.R., Kornberg R.D., Laurent B.C. Mol. Cell. Biol. 17:3323-3334(1997) [PubMed: 9154831] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN THE RSC COMPLEX, INTERACTION WITH STH1, PHOSPHORYLATION.
[4]	"Histone octamer transfer by a chromatin-remodeling complex." Lorch Y., Zhang M., Kornberg R.D. Cell 96:389-392(1999) [PubMed: 10025404] [Abstract] Cited for: FUNCTION OF THE RSC COMPLEX.
[5]	"Transcriptional repression of the yeast CHA1 gene requires the chromatin-remodeling complex RSC." Moreira J.M.A., Holmberg S. EMBO J. 18:2836-2844(1999) [PubMed: 10329629] [Abstract] Cited for: FUNCTION OF THE RSC COMPLEX.
[6]	"Two functionally distinct forms of the RSC nucleosome-remodeling complex, containing essential AT hook, BAH, and bromodomains." Cairns B.R., Schlichter A., Erdjument-Bromage H., Tempst P., Kornberg R.D., Winston F. Mol. Cell 4:715-723(1999) [PubMed: 10619019] [Abstract] Cited for: COMPOSITION OF THE RSC COMPLEX.
[7]	"Chromatin remodeling by RSC involves ATP-dependent DNA translocation." Saha A., Wittmeyer J., Cairns B.R. Genes Dev. 16:2120-2134(2002) [PubMed: 12183366] [Abstract] Cited for: FUNCTION OF THE RSC COMPLEX.
[8]	"Yeast RSC function is required for organization of the cellular cytoskeleton via an alternative PKC1 pathway." Chai B., Hsu J.-M., Du J., Laurent B.C. Genetics 161:575-584(2002) [PubMed: 12072455] [Abstract] Cited for: FUNCTION OF THE RSC COMPLEX.
[9]	"The yeast RSC chromatin-remodeling complex is required for kinetochore function in chromosome segregation." Hsu J.-M., Huang J., Meluh P.B., Laurent B.C. Mol. Cell. Biol. 23:3202-3215(2003) [PubMed: 12697820] [Abstract] Cited for: FUNCTION OF THE RSC COMPLEX, SUBCELLULAR LOCATION, INTERACTION OF THE RSC COMPLEX WITH HISTONES.
[10]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[11]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-78, MASS SPECTROMETRY.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
	U20618 Genomic DNA. Translation: AAB64513.1.
PIR	S53399.
RefSeq	NP_013425.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:1898N.
IntAct	Q06168. 28 interactions.
Proteomic databases
PeptideAtlas	Q06168.
Genome annotation databases
Ensembl	YLR321C. Saccharomyces cerevisiae. [Contig view]
GeneID	851032.
GenomeReviews	Gene locus YLR321C in contig Y13138_GR.
KEGG	sce:YLR321C.
NMPDR	fig\|4932.3.peg.4445.
Organism-specific databases
CYGD	YLR321c.
SGD	S000004313. SFH1.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	Q06168.
OMA	Q06168. HVIINLT.
Gene expression databases
GermOnline	YLR321C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR006939. SNF5. IPR017393. SWI_SNF_chromatin_remodel_cplx. [Graphical view]
PANTHER	PTHR10019. SNF5. 1 hit.
Pfam	PF04855. SNF5. 1 hit. [Graphical view]
PIRSF	PIRSF038126. SWI_SNF. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	967619.

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Entry information

Entry name

SFH1_YEAST

Accession

Primary (citable) accession number: Q06168

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents