Q06163 (TERT_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Telomerase reverse transcriptase
EC=2.7.7.49

Alternative name(s):
Telomerase catalytic subunit

Gene names

Name:	EST2
Ordered Locus Names:	YLR318W
ORF Names:	L8543.12

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	884 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. It elongates telomeres. It is a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme.
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
Subcellular location	Nucleus. Chromosome › telomere.
Miscellaneous	Deletion causes telomere shortening and senescence.
Sequence similarities	Belongs to the reverse transcriptase family. Telomerase subfamily. Contains 1 reverse transcriptase domain.

Ontologies

Keywords
Cellular component	Chromosome Nucleus Telomere
Ligand	DNA-binding Magnesium Metal-binding
Molecular function	Nucleotidyltransferase RNA-directed DNA polymerase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	replicative cell aging Inferred from genetic interaction PubMed 18627461. Source: SGD telomere maintenance via telomerase Inferred from mutant phenotype PubMed 15813705PubMed 8978029. Source: SGD
Cellular_component	chromosome, telomeric region Inferred from electronic annotation. Source: UniProtKB-SubCell nucleolus Inferred from direct assay PubMed 12101098. Source: SGD telomerase catalytic core complex Inferred from physical interaction PubMed 15813705. Source: SGD
Molecular_function	RNA binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW telomeric DNA binding Inferred from direct assay PubMed 17656141. Source: SGD telomeric template RNA reverse transcriptase activity Traceable author statement PubMed 11884619. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 884	884	Telomerase reverse transcriptase		PRO_0000054932
Regions
	Domain	422 – 725	304	Reverse transcriptase
Sites
	Metal binding	530	1	Magnesium; catalytic By similarity
	Metal binding	670	1	Magnesium; catalytic By similarity
	Metal binding	671	1	Magnesium; catalytic By similarity
Amino acid modifications
	Modified residue	537	1	Phosphoserine Ref.4
Experimental info
	Sequence conflict	162	1	A → V in AAB64520. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q06163 [UniParc]. Last modified September 21, 2011. Version 2. Checksum: 84540735E3CD40F5	FASTA	884	102,635
	10 20 30 40 50 60 MKILFEFIQD KLDIDLQTNS TYKENLKCGH FNGLDEILTT CFALPNSRKI ALPCLPGDLS 70 80 90 100 110 120 HKAVIDHCII YLLTGELYNN VLTFGYKIAR NEDVNNSLFC HSANVNVTLL KGAAWKMFHS 130 140 150 160 170 180 LVGTYAFVDL LINYTVIQFN GQFFTQIVGN RCNEPHLPPK WAQRSSSSSA TAAQIKQLTE 190 200 210 220 230 240 PVTNKQFLHK LNINSSSFFP YSKILPSSSS IKKLTDLREA IFPTNLVKIP QRLKVRINLT 250 260 270 280 290 300 LQKLLKRHKR LNYVSILNSI CPPLEGTVLD LSHLSRQSPK ERVLKFIIVI LQKLLPQEMF 310 320 330 340 350 360 GSKKNKGKII KNLNLLLSLP LNGYLPFDSL LKKLRLKDFR WLFISDIWFT KHNFENLNQL 370 380 390 400 410 420 AICFISWLFR QLIPKIIQTF FYCTEISSTV TIVYFRHDTW NKLITPFIVE YFKTYLVENN 430 440 450 460 470 480 VCRNHNSYTL SNFNHSKMRI IPKKSNNEFR IIAIPCRGAD EEEFTIYKEN HKNAIQPTQK 490 500 510 520 530 540 ILEYLRNKRP TSFTKIYSPT QIADRIKEFK QRLLKKFNNV LPELYFMKFD VKSCYDSIPR 550 560 570 580 590 600 MECMRILKDA LKNENGFFVR SQYFFNTNTG VLKLFNVVNA SRVPKPYELY IDNVRTVHLS 610 620 630 640 650 660 NQDVINVVEM EIFKTALWVE DKCYIREDGL FQGSSLSAPI VDLVYDDLLE FYSEFKASPS 670 680 690 700 710 720 QDTLILKLAD DFLIISTDQQ QVINIKKLAM GGFQKYNAKA NRDKILAVSS QSDDDTVIQF 730 740 750 760 770 780 CAMHIFVKEL EVWKHSSTMN NFHIRSKSSK GIFRSLIALF NTRISYKTID TNLNSTNTVL 790 800 810 820 830 840 MQIDHVVKNI SECYKSAFKD LSINVTQNMQ FHSFLQRIIE MTVSGCPITK CDPLIEYEVR 850 860 870 880 FTILNGFLES LSSNTSKFKD NIILLRKEIQ HLQAYIYIYI HIVN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D. Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 162. Strain: ATCC 204508 / S288c.
[3]	"Reverse transcriptase motifs in the catalytic subunit of telomerase." Lingner J., Hughes T.R., Shevchenko A., Mann M., Lundblad V., Cech T.R. Science 276:561-567(1997) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[4]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-537, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U20618 Genomic DNA. Translation: AAB64520.1. BK006945 Genomic DNA. Translation: DAA09627.2.
PIR	S53396.
RefSeq	NP_013422.2. NM_001182207.2.
3D structure databases
ProteinModelPortal	Q06163.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-7548N.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YLR318W; YLR318W; YLR318W.
GeneID	851028.
KEGG	sce:YLR318W.
Organism-specific databases
CYGD	YLR318w.
SGD	S000004310. EST2.
Phylogenomic databases
eggNOG	NOG276584.
GeneTree	ENSGT00390000018531.
HOGENOM	HOG000141949.
KO	K11126.
OMA	SCYDSIP.
OrthoDB	EOG41NXVP.
Gene expression databases
ArrayExpress	Q06163.
Genevestigator	Q06163.
GermOnline	YLR318W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR000477. RVT. IPR021891. Telomerase_RBD. IPR003545. Telomerase_RT. [Graphical view]
Pfam	PF00078. RVT_1. 1 hit. PF12009. Telomerase_RBD. 1 hit. [Graphical view]
PRINTS	PR01365. TELOMERASERT.
SMART	SM00975. Telomerase_RBD. 1 hit. [Graphical view]
PROSITE	PS50878. RT_POL. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	967610.

Entry information

Entry name

TERT_YEAST

Accession

Primary (citable) accession number: Q06163
Secondary accession number(s): D6VYW1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	September 21, 2011
Last modified:	May 1, 2013
This is version 100 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program