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Q06163

- TERT_YEAST

UniProt

Q06163 - TERT_YEAST

Protein

Telomerase reverse transcriptase

Gene

EST2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 2 (21 Sep 2011)
      Previous versions | rss
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    Functioni

    Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. It elongates telomeres. It is a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme.

    Catalytic activityi

    Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi530 – 5301Magnesium; catalyticPROSITE-ProRule annotation
    Metal bindingi670 – 6701Magnesium; catalyticPROSITE-ProRule annotation
    Metal bindingi671 – 6711Magnesium; catalyticPROSITE-ProRule annotation

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. RNA binding Source: InterPro
    3. telomeric DNA binding Source: SGD
    4. telomeric template RNA reverse transcriptase activity Source: SGD

    GO - Biological processi

    1. replicative cell aging Source: SGD
    2. telomere maintenance via telomerase Source: SGD

    Keywords - Molecular functioni

    Nucleotidyltransferase, RNA-directed DNA polymerase, Transferase

    Keywords - Ligandi

    DNA-binding, Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32402-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Telomerase reverse transcriptase (EC:2.7.7.49)
    Alternative name(s):
    Telomerase catalytic subunit
    Gene namesi
    Name:EST2
    Ordered Locus Names:YLR318W
    ORF Names:L8543.12
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XII

    Organism-specific databases

    CYGDiYLR318w.
    SGDiS000004310. EST2.

    Subcellular locationi

    GO - Cellular componenti

    1. chromosome, telomeric region Source: UniProtKB-SubCell
    2. nucleolus Source: SGD
    3. nucleus Source: SGD
    4. telomerase catalytic core complex Source: SGD
    5. telomerase holoenzyme complex Source: SGD

    Keywords - Cellular componenti

    Chromosome, Nucleus, Telomere

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 884884Telomerase reverse transcriptasePRO_0000054932Add
    BLAST

    Expressioni

    Gene expression databases

    GenevestigatoriQ06163.

    Interactioni

    Protein-protein interaction databases

    BioGridi31582. 40 interactions.
    DIPiDIP-7548N.
    IntActiQ06163. 4 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ06163.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini422 – 725304Reverse transcriptasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 reverse transcriptase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG276584.
    GeneTreeiENSGT00390000018531.
    HOGENOMiHOG000141949.
    KOiK11126.
    OMAiTINIVRI.
    OrthoDBiEOG7VTDWJ.

    Family and domain databases

    InterProiIPR000477. RT_dom.
    IPR021891. Telomerase_RBD.
    IPR003545. Telomerase_RT.
    [Graphical view]
    PfamiPF00078. RVT_1. 1 hit.
    PF12009. Telomerase_RBD. 1 hit.
    [Graphical view]
    PRINTSiPR01365. TELOMERASERT.
    SMARTiSM00975. Telomerase_RBD. 1 hit.
    [Graphical view]
    PROSITEiPS50878. RT_POL. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q06163-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKILFEFIQD KLDIDLQTNS TYKENLKCGH FNGLDEILTT CFALPNSRKI    50
    ALPCLPGDLS HKAVIDHCII YLLTGELYNN VLTFGYKIAR NEDVNNSLFC 100
    HSANVNVTLL KGAAWKMFHS LVGTYAFVDL LINYTVIQFN GQFFTQIVGN 150
    RCNEPHLPPK WAQRSSSSSA TAAQIKQLTE PVTNKQFLHK LNINSSSFFP 200
    YSKILPSSSS IKKLTDLREA IFPTNLVKIP QRLKVRINLT LQKLLKRHKR 250
    LNYVSILNSI CPPLEGTVLD LSHLSRQSPK ERVLKFIIVI LQKLLPQEMF 300
    GSKKNKGKII KNLNLLLSLP LNGYLPFDSL LKKLRLKDFR WLFISDIWFT 350
    KHNFENLNQL AICFISWLFR QLIPKIIQTF FYCTEISSTV TIVYFRHDTW 400
    NKLITPFIVE YFKTYLVENN VCRNHNSYTL SNFNHSKMRI IPKKSNNEFR 450
    IIAIPCRGAD EEEFTIYKEN HKNAIQPTQK ILEYLRNKRP TSFTKIYSPT 500
    QIADRIKEFK QRLLKKFNNV LPELYFMKFD VKSCYDSIPR MECMRILKDA 550
    LKNENGFFVR SQYFFNTNTG VLKLFNVVNA SRVPKPYELY IDNVRTVHLS 600
    NQDVINVVEM EIFKTALWVE DKCYIREDGL FQGSSLSAPI VDLVYDDLLE 650
    FYSEFKASPS QDTLILKLAD DFLIISTDQQ QVINIKKLAM GGFQKYNAKA 700
    NRDKILAVSS QSDDDTVIQF CAMHIFVKEL EVWKHSSTMN NFHIRSKSSK 750
    GIFRSLIALF NTRISYKTID TNLNSTNTVL MQIDHVVKNI SECYKSAFKD 800
    LSINVTQNMQ FHSFLQRIIE MTVSGCPITK CDPLIEYEVR FTILNGFLES 850
    LSSNTSKFKD NIILLRKEIQ HLQAYIYIYI HIVN 884
    Length:884
    Mass (Da):102,635
    Last modified:September 21, 2011 - v2
    Checksum:i84540735E3CD40F5
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti162 – 1621A → V in AAB64520. (PubMed:9169871)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U20618 Genomic DNA. Translation: AAB64520.1.
    BK006945 Genomic DNA. Translation: DAA09627.2.
    PIRiS53396.
    RefSeqiNP_013422.2. NM_001182207.2.

    Genome annotation databases

    EnsemblFungiiYLR318W; YLR318W; YLR318W.
    GeneIDi851028.
    KEGGisce:YLR318W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U20618 Genomic DNA. Translation: AAB64520.1 .
    BK006945 Genomic DNA. Translation: DAA09627.2 .
    PIRi S53396.
    RefSeqi NP_013422.2. NM_001182207.2.

    3D structure databases

    ProteinModelPortali Q06163.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31582. 40 interactions.
    DIPi DIP-7548N.
    IntActi Q06163. 4 interactions.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YLR318W ; YLR318W ; YLR318W .
    GeneIDi 851028.
    KEGGi sce:YLR318W.

    Organism-specific databases

    CYGDi YLR318w.
    SGDi S000004310. EST2.

    Phylogenomic databases

    eggNOGi NOG276584.
    GeneTreei ENSGT00390000018531.
    HOGENOMi HOG000141949.
    KOi K11126.
    OMAi TINIVRI.
    OrthoDBi EOG7VTDWJ.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32402-MONOMER.

    Miscellaneous databases

    NextBioi 967610.
    PROi Q06163.

    Gene expression databases

    Genevestigatori Q06163.

    Family and domain databases

    InterProi IPR000477. RT_dom.
    IPR021891. Telomerase_RBD.
    IPR003545. Telomerase_RT.
    [Graphical view ]
    Pfami PF00078. RVT_1. 1 hit.
    PF12009. Telomerase_RBD. 1 hit.
    [Graphical view ]
    PRINTSi PR01365. TELOMERASERT.
    SMARTi SM00975. Telomerase_RBD. 1 hit.
    [Graphical view ]
    PROSITEi PS50878. RT_POL. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
      Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
      , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
      Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 162.
      Strain: ATCC 204508 / S288c.
    3. "Reverse transcriptase motifs in the catalytic subunit of telomerase."
      Lingner J., Hughes T.R., Shevchenko A., Mann M., Lundblad V., Cech T.R.
      Science 276:561-567(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.

    Entry informationi

    Entry nameiTERT_YEAST
    AccessioniPrimary (citable) accession number: Q06163
    Secondary accession number(s): D6VYW1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: September 21, 2011
    Last modified: October 1, 2014
    This is version 112 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Deletion causes telomere shortening and senescence.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XII
      Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

    External Data

    Dasty 3