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Protein

Telomerase reverse transcriptase

Gene

EST2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. It elongates telomeres. It is a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme.

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi530 – 5301Magnesium; catalyticPROSITE-ProRule annotation
Metal bindingi670 – 6701Magnesium; catalyticPROSITE-ProRule annotation
Metal bindingi671 – 6711Magnesium; catalyticPROSITE-ProRule annotation

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • telomeric DNA binding Source: SGD
  • telomeric template RNA reverse transcriptase activity Source: SGD

GO - Biological processi

  • replicative cell aging Source: SGD
  • telomere maintenance via telomerase Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, RNA-directed DNA polymerase, Transferase

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32402-MONOMER.
ReactomeiREACT_277124. Telomere Extension By Telomerase.

Names & Taxonomyi

Protein namesi
Recommended name:
Telomerase reverse transcriptase (EC:2.7.7.49)
Alternative name(s):
Telomerase catalytic subunit
Gene namesi
Name:EST2
Ordered Locus Names:YLR318W
ORF Names:L8543.12
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome XII

Organism-specific databases

CYGDiYLR318w.
EuPathDBiFungiDB:YLR318W.
SGDiS000004310. EST2.

Subcellular locationi

GO - Cellular componenti

  • chromosome, telomeric region Source: UniProtKB-SubCell
  • nucleolus Source: SGD
  • nucleus Source: SGD
  • telomerase catalytic core complex Source: SGD
  • telomerase holoenzyme complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Nucleus, Telomere

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 884884Telomerase reverse transcriptasePRO_0000054932Add
BLAST

Interactioni

Subunit structurei

Catalytic subunit of the telomerase holoenzyme complex composed minimally of EST2 and the telomerase RNA template component.By similarity

Protein-protein interaction databases

BioGridi31582. 40 interactions.
DIPiDIP-7548N.
IntActiQ06163. 4 interactions.
STRINGi4932.YLR318W.

Structurei

3D structure databases

ProteinModelPortaliQ06163.
SMRiQ06163. Positions 243-403.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini422 – 725304Reverse transcriptasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 reverse transcriptase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG276584.
GeneTreeiENSGT00390000018531.
HOGENOMiHOG000141949.
InParanoidiQ06163.
KOiK11126.
OMAiTINIVRI.
OrthoDBiEOG7VTDWJ.

Family and domain databases

InterProiIPR000477. RT_dom.
IPR021891. Telomerase_RBD.
IPR003545. Telomerase_RT.
[Graphical view]
PfamiPF00078. RVT_1. 1 hit.
PF12009. Telomerase_RBD. 1 hit.
[Graphical view]
PRINTSiPR01365. TELOMERASERT.
SMARTiSM00975. Telomerase_RBD. 1 hit.
[Graphical view]
PROSITEiPS50878. RT_POL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q06163-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKILFEFIQD KLDIDLQTNS TYKENLKCGH FNGLDEILTT CFALPNSRKI
60 70 80 90 100
ALPCLPGDLS HKAVIDHCII YLLTGELYNN VLTFGYKIAR NEDVNNSLFC
110 120 130 140 150
HSANVNVTLL KGAAWKMFHS LVGTYAFVDL LINYTVIQFN GQFFTQIVGN
160 170 180 190 200
RCNEPHLPPK WAQRSSSSSA TAAQIKQLTE PVTNKQFLHK LNINSSSFFP
210 220 230 240 250
YSKILPSSSS IKKLTDLREA IFPTNLVKIP QRLKVRINLT LQKLLKRHKR
260 270 280 290 300
LNYVSILNSI CPPLEGTVLD LSHLSRQSPK ERVLKFIIVI LQKLLPQEMF
310 320 330 340 350
GSKKNKGKII KNLNLLLSLP LNGYLPFDSL LKKLRLKDFR WLFISDIWFT
360 370 380 390 400
KHNFENLNQL AICFISWLFR QLIPKIIQTF FYCTEISSTV TIVYFRHDTW
410 420 430 440 450
NKLITPFIVE YFKTYLVENN VCRNHNSYTL SNFNHSKMRI IPKKSNNEFR
460 470 480 490 500
IIAIPCRGAD EEEFTIYKEN HKNAIQPTQK ILEYLRNKRP TSFTKIYSPT
510 520 530 540 550
QIADRIKEFK QRLLKKFNNV LPELYFMKFD VKSCYDSIPR MECMRILKDA
560 570 580 590 600
LKNENGFFVR SQYFFNTNTG VLKLFNVVNA SRVPKPYELY IDNVRTVHLS
610 620 630 640 650
NQDVINVVEM EIFKTALWVE DKCYIREDGL FQGSSLSAPI VDLVYDDLLE
660 670 680 690 700
FYSEFKASPS QDTLILKLAD DFLIISTDQQ QVINIKKLAM GGFQKYNAKA
710 720 730 740 750
NRDKILAVSS QSDDDTVIQF CAMHIFVKEL EVWKHSSTMN NFHIRSKSSK
760 770 780 790 800
GIFRSLIALF NTRISYKTID TNLNSTNTVL MQIDHVVKNI SECYKSAFKD
810 820 830 840 850
LSINVTQNMQ FHSFLQRIIE MTVSGCPITK CDPLIEYEVR FTILNGFLES
860 870 880
LSSNTSKFKD NIILLRKEIQ HLQAYIYIYI HIVN
Length:884
Mass (Da):102,635
Last modified:September 21, 2011 - v2
Checksum:i84540735E3CD40F5
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti162 – 1621A → V in AAB64520 (PubMed:9169871).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U20618 Genomic DNA. Translation: AAB64520.1.
BK006945 Genomic DNA. Translation: DAA09627.2.
PIRiS53396.
RefSeqiNP_013422.2. NM_001182207.2.

Genome annotation databases

EnsemblFungiiYLR318W; YLR318W; YLR318W.
GeneIDi851028.
KEGGisce:YLR318W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U20618 Genomic DNA. Translation: AAB64520.1.
BK006945 Genomic DNA. Translation: DAA09627.2.
PIRiS53396.
RefSeqiNP_013422.2. NM_001182207.2.

3D structure databases

ProteinModelPortaliQ06163.
SMRiQ06163. Positions 243-403.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31582. 40 interactions.
DIPiDIP-7548N.
IntActiQ06163. 4 interactions.
STRINGi4932.YLR318W.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR318W; YLR318W; YLR318W.
GeneIDi851028.
KEGGisce:YLR318W.

Organism-specific databases

CYGDiYLR318w.
EuPathDBiFungiDB:YLR318W.
SGDiS000004310. EST2.

Phylogenomic databases

eggNOGiNOG276584.
GeneTreeiENSGT00390000018531.
HOGENOMiHOG000141949.
InParanoidiQ06163.
KOiK11126.
OMAiTINIVRI.
OrthoDBiEOG7VTDWJ.

Enzyme and pathway databases

BioCyciYEAST:G3O-32402-MONOMER.
ReactomeiREACT_277124. Telomere Extension By Telomerase.

Miscellaneous databases

NextBioi967610.
PROiQ06163.

Family and domain databases

InterProiIPR000477. RT_dom.
IPR021891. Telomerase_RBD.
IPR003545. Telomerase_RT.
[Graphical view]
PfamiPF00078. RVT_1. 1 hit.
PF12009. Telomerase_RBD. 1 hit.
[Graphical view]
PRINTSiPR01365. TELOMERASERT.
SMARTiSM00975. Telomerase_RBD. 1 hit.
[Graphical view]
PROSITEiPS50878. RT_POL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
    Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
    , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
    Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 162.
    Strain: ATCC 204508 / S288c.
  3. "Reverse transcriptase motifs in the catalytic subunit of telomerase."
    Lingner J., Hughes T.R., Shevchenko A., Mann M., Lundblad V., Cech T.R.
    Science 276:561-567(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.

Entry informationi

Entry nameiTERT_YEAST
AccessioniPrimary (citable) accession number: Q06163
Secondary accession number(s): D6VYW1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: September 21, 2011
Last modified: June 24, 2015
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Deletion causes telomere shortening and senescence.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.