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Protein

Central kinetochore subunit NKP2

Gene

NKP2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the central kinetochore, which mediates the attachment of the centromere to the mitotic spindle by forming essential interactions between the microtubule-associated outer kinetochore proteins and the centromere-associated inner kinetochore proteins. Required for establishing bipolar spindle-microtubule attachments and proper chromosome segregation.

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • chromosome segregation Source: SGD
  • meiotic cell cycle Source: UniProtKB-KW
  • mitotic nuclear division Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Meiosis, Mitosis

Enzyme and pathway databases

BioCyciYEAST:G3O-32401-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Central kinetochore subunit NKP2
Alternative name(s):
Non-essential kinetochore protein 2
Gene namesi
Name:NKP2
Ordered Locus Names:YLR315W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR315W.
SGDiS000004307. NKP2.

Subcellular locationi

GO - Cellular componenti

  • kinetochore Source: SGD
  • Mis6-Sim4 complex Source: GO_Central
  • nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000968681 – 153Central kinetochore subunit NKP2Add BLAST153

Proteomic databases

PRIDEiQ06162.

Interactioni

Subunit structurei

Component of the central kinetochore CTF19 complex, which is composed of at least AME1, CHL4, CTF3, CTF19, IML3, MCM16, MCM21, MCM22, NKP1, NKP2 and OKP1.

Binary interactionsi

WithEntry#Exp.IntActNotes
NKP1Q124933EBI-34256,EBI-35840

Protein-protein interaction databases

BioGridi31580. 119 interactors.
DIPiDIP-1939N.
IntActiQ06162. 8 interactors.
MINTiMINT-389657.

Structurei

3D structure databases

ProteinModelPortaliQ06162.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili86 – 128Sequence analysisAdd BLAST43

Keywords - Domaini

Coiled coil

Phylogenomic databases

InParanoidiQ06162.
OMAiHESEPDE.
OrthoDBiEOG092C5Z03.

Family and domain databases

InterProiIPR018565. Kinetochore_su_NPK2.
[Graphical view]
PfamiPF09447. Cnl2_NKP2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q06162-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNSEQLLHNY VSDSLLTTLI SFQEFKQQLQ SYTSDEQQLQ HWYELLQARD
60 70 80 90 100
ARVTSELEAR IKQFFITLRS RLLRFLESEQ LSHSLSLETL IDALYKINDL
110 120 130 140 150
LQQRLQILDD AIQEKTSELA EFENMVRSPS AGDNAIPGLL QIIQSYINLL

EEN
Length:153
Mass (Da):17,862
Last modified:November 1, 1996 - v1
Checksum:i1445E11838649D70
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U20618 Genomic DNA. Translation: AAB64519.1.
AY692848 Genomic DNA. Translation: AAT92867.1.
BK006945 Genomic DNA. Translation: DAA09625.1.
PIRiS53394.
RefSeqiNP_013419.1. NM_001182204.1.

Genome annotation databases

EnsemblFungiiYLR315W; YLR315W; YLR315W.
GeneIDi851025.
KEGGisce:YLR315W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U20618 Genomic DNA. Translation: AAB64519.1.
AY692848 Genomic DNA. Translation: AAT92867.1.
BK006945 Genomic DNA. Translation: DAA09625.1.
PIRiS53394.
RefSeqiNP_013419.1. NM_001182204.1.

3D structure databases

ProteinModelPortaliQ06162.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31580. 119 interactors.
DIPiDIP-1939N.
IntActiQ06162. 8 interactors.
MINTiMINT-389657.

Proteomic databases

PRIDEiQ06162.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR315W; YLR315W; YLR315W.
GeneIDi851025.
KEGGisce:YLR315W.

Organism-specific databases

EuPathDBiFungiDB:YLR315W.
SGDiS000004307. NKP2.

Phylogenomic databases

InParanoidiQ06162.
OMAiHESEPDE.
OrthoDBiEOG092C5Z03.

Enzyme and pathway databases

BioCyciYEAST:G3O-32401-MONOMER.

Miscellaneous databases

PROiQ06162.

Family and domain databases

InterProiIPR018565. Kinetochore_su_NPK2.
[Graphical view]
PfamiPF09447. Cnl2_NKP2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNKP2_YEAST
AccessioniPrimary (citable) accession number: Q06162
Secondary accession number(s): D6VYV9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1630 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.