Q06162 (NKP2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Central kinetochore subunit NKP2

Alternative name(s):
Non-essential kinetochore protein 2

Gene names

Name:	NKP2
Ordered Locus Names:	YLR315W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	153 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the central kinetochore, which mediates the attachment of the centromere to the mitotic spindle by forming essential interactions between the microtubule-associated outer kinetochore proteins and the centromere-associated inner kinetochore proteins. Required for establishing bipolar spindle-microtubule attachments and proper chromosome segregation.
Subunit structure	Component of the central kinetochore CTF19 complex, which is composed of at least AME1, CHL4, CTF3, CTF19, IML3, MCM16, MCM21, MCM22, NKP1, NKP2 and OKP1.
Subcellular location	Nucleus. Chromosome › centromere › kinetochore. Note: Associated with kinetochores. Ref.4 Ref.5
Miscellaneous	Present with 1630 molecules/cell in log phase SD medium. Ref.6

Ontologies

Keywords
Biological process	Cell cycle Cell division Meiosis Mitosis
Cellular component	Centromere Chromosome Kinetochore Nucleus
Domain	Coiled coil
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell division Inferred from electronic annotation. Source: UniProtKB-KW meiosis Inferred from electronic annotation. Source: UniProtKB-KW mitosis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	condensed chromosome kinetochore Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
NKP1	Q12493	3	EBI-34256,EBI-35840

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 153	153	Central kinetochore subunit NKP2		PRO_0000096868
Regions
	Coiled coil	86 – 128	43	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q06162 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 1445E11838649D70	FASTA	153	17,862
	10 20 30 40 50 60 MNSEQLLHNY VSDSLLTTLI SFQEFKQQLQ SYTSDEQQLQ HWYELLQARD ARVTSELEAR 70 80 90 100 110 120 IKQFFITLRS RLLRFLESEQ LSHSLSLETL IDALYKINDL LQQRLQILDD AIQEKTSELA 130 140 150 EFENMVRSPS AGDNAIPGLL QIIQSYINLL EEN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D. Nature 387:87-90(1997) [PubMed: 9169871] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Phospho-regulation of kinetochore-microtubule attachments by the Aurora kinase Ipl1p." Cheeseman I.M., Anderson S., Jwa M., Green E.M., Kang J.-S., Yates J.R. III, Chan C.S.M., Drubin D.G., Barnes G. Cell 111:163-172(2002) [PubMed: 12408861] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, COMPONENT OF CTF19 COMPLEX, SUBCELLULAR LOCATION.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U20618 Genomic DNA. Translation: AAB64519.1. AY692848 Genomic DNA. Translation: AAT92867.1. BK006945 Genomic DNA. Translation: DAA09625.1.
PIR	S53394.
RefSeq	NP_013419.1. NM_001182204.1.
3D structure databases
ProteinModelPortal	Q06162.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1939N.
IntAct	Q06162. 14 interactions.
MINT	MINT-389657.
STRING	Q06162.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YLR315W; YLR315W; YLR315W.
GeneID	851025.
KEGG	sce:YLR315W.
NMPDR	fig|4932.3.peg.4439.
Organism-specific databases
CYGD	YLR315w.
SGD	S000004307. NKP2.
Phylogenomic databases
eggNOG	fuNOG13668.
OMA	FLESEQL.
Gene expression databases
ArrayExpress	Q06162.
Genevestigator	Q06162.
GermOnline	YLR315W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR018565. Kinetochore_su_NPK2. [Graphical view]
Pfam	PF09447. Cnl2_NKP2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	967604.

Entry information

Entry name

NKP2_YEAST

Accession

Primary (citable) accession number: Q06162
Secondary accession number(s): D6VYV9

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program