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Protein

Non-homologous end-joining protein 1

Gene

NEJ1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in non-homologous end joining (NHEJ). Facilitates the transport of LIF1 into the nucleus, where it can interact with DNA ligase DNL4 to repair double-strand breaks (DSB). Mediates mating-type regulation of NHEJ. Prevents chromosome circularisation by NHEJ in absence of telomerase.6 Publications

Miscellaneous

Present with 377 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • DNA end binding Source: SGD

GO - Biological processi

  • double-strand break repair via nonhomologous end joining Source: SGD
  • double-strand break repair via single-strand annealing Source: SGD

Keywordsi

Biological processDNA damage, DNA repair

Enzyme and pathway databases

BioCyciYEAST:G3O-32365-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Non-homologous end-joining protein 1
Gene namesi
Name:NEJ1
Synonyms:LIF2
Ordered Locus Names:YLR265C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR265C.
SGDiS000004255. NEJ1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei27 – 47HelicalSequence analysisAdd BLAST21
Transmembranei129 – 149HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • DNA ligase IV complex Source: SGD
  • integral component of membrane Source: UniProtKB-KW
  • nonhomologous end joining complex Source: GO_Central
  • nuclear membrane Source: UniProtKB-SubCell
  • nucleus Source: SGD

Keywords - Cellular componenti

Cytoplasm, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002686891 – 342Non-homologous end-joining protein 1Add BLAST342

Proteomic databases

MaxQBiQ06148.
PRIDEiQ06148.

PTM databases

iPTMnetiQ06148.

Expressioni

Inductioni

Repressed in diploid cells.3 Publications

Interactioni

Protein-protein interaction databases

BioGridi31533. 97 interactors.
DIPiDIP-4776N.
IntActiQ06148. 2 interactors.
MINTiMINT-521085.
STRINGi4932.YLR265C.

Structurei

3D structure databases

ProteinModelPortaliQ06148.
SMRiQ06148.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the XLF family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

InParanoidiQ06148.
KOiK10983.
OrthoDBiEOG092C3825.

Family and domain databases

InterProiView protein in InterPro
IPR015381. XLF_fam.
PfamiView protein in Pfam
PF09302. XLF. 1 hit.

Sequencei

Sequence statusi: Complete.

Q06148-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDSELKGQQL SDAEWCVKKI NGEGNCLLLF LPMSSPTTIV MIVLVSLERL
60 70 80 90 100
VPYVFKLSQT QLSQQCQSQG FTDSISLNLI KLKLMDILQA PQEINQIGLV
110 120 130 140 150
DSNLVFSFDV SADITVSINS VPSHVTKDMF YMILQSLCML LLKLVNLSTQ
160 170 180 190 200
YHYVQRDILN EKQKCLDFLL ISLRDLDGGS KVISQWAPEN SKNYESLQQC
210 220 230 240 250
TDDDIIKKLL HKGKFQHQEF LADSLKTLLS LRNKFQDVSR FEESGELNKK
260 270 280 290 300
ERVRFPAVNH FYNDDFELQA DPTNEARPNS RGKIKPKTDF KPKSRESSTS
310 320 330 340
SQLRLENFSE SEATPEKTKS SSSLVEEYPQ KKRKFGKVRI KN
Length:342
Mass (Da):39,116
Last modified:November 1, 1996 - v1
Checksum:i5E09B5F851CF03B9
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti17V → I in strain: DBVPG1135, DBVPG1373, DBVPG1378, DBVPG1788, DBVPG1794, DBVPG1853, DBVPG3051, DBVPG6044, DBVPG6763, DBVPG6765, SK1, Y55 and YPS128. 1 Publication1
Natural varianti21N → I in strain: DBVPG1135, DBVPG1373, DBVPG1378, DBVPG1788, DBVPG1794, DBVPG1853, DBVPG3051, DBVPG6763, DBVPG6765 and SK1. 1 Publication1
Natural varianti65Q → E in strain: YPS128. 1 Publication1
Natural varianti105V → A in strain: YPS128. 1 Publication1
Natural varianti161E → Q in strain: DBVPG1135, DBVPG1373, DBVPG1378, DBVPG1788, DBVPG1794, DBVPG1853, DBVPG3051, DBVPG6763, DBVPG6765 and SK1. 1 Publication1
Natural varianti204D → G in strain: DBVPG6044 and Y55. 1 Publication1
Natural varianti231L → F in strain: DBVPG1135, DBVPG1373, DBVPG1378, DBVPG1788, DBVPG1794, DBVPG1853, DBVPG3051, DBVPG6763, DBVPG6765 and SK1. 1 Publication1
Natural varianti249K → E in strain: DBVPG1135, DBVPG1373, DBVPG1378, DBVPG1788, DBVPG1794, DBVPG1853, DBVPG3051, DBVPG6763, DBVPG6765 and SK1. 1 Publication1
Natural varianti270A → V in strain: DBVPG1135, DBVPG1373, DBVPG1378, DBVPG1788, DBVPG1794, DBVPG1853, DBVPG3051, DBVPG6763, DBVPG6765 and SK1. 1 Publication1
Natural varianti281R → C in strain: DBVPG1135, DBVPG1373, DBVPG1378, DBVPG1788, DBVPG1794, DBVPG1853, DBVPG3051, DBVPG6763, DBVPG6765 and SK1. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM296374 Genomic DNA. Translation: CAL35943.1.
AM296375 Genomic DNA. Translation: CAL35942.1.
AM296376 Genomic DNA. Translation: CAL35941.1.
AM296377 Genomic DNA. Translation: CAL35940.1.
AM296378 Genomic DNA. Translation: CAL35939.1.
AM296379 Genomic DNA. Translation: CAL35938.1.
AM296380 Genomic DNA. Translation: CAL35937.1.
AM296381 Genomic DNA. Translation: CAL35936.1.
AM296382 Genomic DNA. Translation: CAL36078.1.
AM296383 Genomic DNA. Translation: CAL35935.1.
AM296384 Genomic DNA. Translation: CAL35934.1.
AM296385 Genomic DNA. Translation: CAL35933.1.
AM296386 Genomic DNA. Translation: CAL35932.1.
U17244 Genomic DNA. Translation: AAB67382.1.
AY557954 Genomic DNA. Translation: AAS56280.1.
BK006945 Genomic DNA. Translation: DAA09579.1.
PIRiS51402.
RefSeqiNP_013367.1. NM_001182152.1.

Genome annotation databases

EnsemblFungiiYLR265C; YLR265C; YLR265C.
GeneIDi850970.
KEGGisce:YLR265C.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiNEJ1_YEAST
AccessioniPrimary (citable) accession number: Q06148
Secondary accession number(s): D6VYR3
, Q0P6Z6, Q0P6Z7, Q0P707
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: November 1, 1996
Last modified: July 5, 2017
This is version 104 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names