Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q06147

- LCB5_YEAST

UniProt

Q06147 - LCB5_YEAST

Protein

Sphingoid long chain base kinase 5

Gene

LCB5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the phosphorylation of the sphingoid long chain bases dihydrosphingosine (DHS or sphinganine) and phytosphingosine (PHS) to form dihydrosphingosine 1-phosphate (DHS-1P) and phytosphingosine 1-phosphate (PHS-1P) respectively. Redundant to LCB4, is only responsible for few percent of the total activity. Involved in the biosynthesis of sphingolipids and ceramides. Involved in heat-induced transient cell cycle arrest. Accumulation of phosphorylated sphingoid long chain bases (LCBPs) stimulates calcium influx and activates calcineurin signaling. Involved in heat-stress resistance.5 Publications

    Catalytic activityi

    ATP + sphinganine = ADP + sphinganine 1-phosphate.
    ATP + phytosphingosine = ADP + phytosphingosine 1-phosphate.

    Kineticsi

    1. KM=2 µM for dihydrosphingosine1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei308 – 3081ATPPROSITE-ProRule annotation
    Active sitei335 – 3351Proton donor/acceptorBy similarity
    Binding sitei340 – 3401ATPPROSITE-ProRule annotation
    Binding sitei434 – 4341ATPPROSITE-ProRule annotation
    Binding sitei440 – 4401ATPPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi276 – 2783ATPPROSITE-ProRule annotation
    Nucleotide bindingi366 – 3683ATPPROSITE-ProRule annotation
    Nucleotide bindingi652 – 6543ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. D-erythro-sphingosine kinase activity Source: SGD
    3. diacylglycerol kinase activity Source: InterPro
    4. NAD+ kinase activity Source: InterPro
    5. sphinganine kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. calcium-mediated signaling Source: SGD
    2. protein kinase C-activating G-protein coupled receptor signaling pathway Source: InterPro
    3. response to heat Source: SGD
    4. sphingolipid metabolic process Source: SGD

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Lipid metabolism, Sphingolipid metabolism

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:YLR260W-MONOMER.
    YEAST:YLR260W-MONOMER.
    ReactomeiREACT_188975. Glycosphingolipid metabolism.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sphingoid long chain base kinase 5 (EC:2.7.1.91)
    Short name:
    LCB kinase 5
    Alternative name(s):
    Sphinganine kinase 5
    Gene namesi
    Name:LCB5
    Ordered Locus Names:YLR260W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XII

    Organism-specific databases

    CYGDiYLR260w.
    SGDiS000004250. LCB5.

    Subcellular locationi

    Golgi apparatus membrane 1 Publication; Peripheral membrane protein 1 Publication

    GO - Cellular componenti

    1. Golgi apparatus Source: SGD
    2. Golgi membrane Source: UniProtKB-SubCell
    3. membrane Source: SGD

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 687687Sphingoid long chain base kinase 5PRO_0000255957Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi91 – 911S-palmitoyl cysteineBy similarity
    Lipidationi94 – 941S-palmitoyl cysteineBy similarity

    Keywords - PTMi

    Lipoprotein, Palmitate

    Proteomic databases

    MaxQBiQ06147.
    PaxDbiQ06147.
    PeptideAtlasiQ06147.

    Expressioni

    Gene expression databases

    GenevestigatoriQ06147.

    Interactioni

    Protein-protein interaction databases

    BioGridi31528. 134 interactions.
    IntActiQ06147. 1 interaction.
    MINTiMINT-2784711.
    STRINGi4932.YLR260W.

    Structurei

    3D structure databases

    ProteinModelPortaliQ06147.
    SMRiQ06147. Positions 271-455, 550-667.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini266 – 405140DAGKcPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni333 – 3364Substrate bindingBy similarity

    Sequence similaritiesi

    Contains 1 DAGKc domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG1597.
    GeneTreeiENSGT00690000101761.
    HOGENOMiHOG000207396.
    KOiK04718.
    OMAiISRTSFQ.
    OrthoDBiEOG7KH9TK.

    Family and domain databases

    InterProiIPR016064. ATP-NAD_kinase_PpnK-typ.
    IPR001206. Diacylglycerol_kinase_cat_dom.
    [Graphical view]
    PfamiPF00781. DAGK_cat. 1 hit.
    [Graphical view]
    SMARTiSM00046. DAGKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF111331. SSF111331. 2 hits.
    PROSITEiPS50146. DAGK. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q06147-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTLKPSKRRK GRSRHSRKKQ ITSAILTEEG IMIKAKPSSP YTYANRMADK    50
    RSRSSIDNIS RTSFQSNISR TSFQSNSDNN SIFETASLIS CVTCLSDTDT 100
    IDRSETSTTD TSKDDLSANP KLHYPSVNGQ LPANTVIPYG RILDARYIEK 150
    EPLHYYDANS SPSSPLSSSM SNISEKCDLD ELESSQKKER KGNSLSRGSN 200
    SSSSLLTSRS PFTKLVEVIF ARPRRHDVVP KRVSLYIDYK PHSSSHLKEE 250
    DDLVEEILKR SYKNTRRNKS IFVIINPFGG KGKAKKLFMT KAKPLLLASR 300
    CSIEVVYTKY PGHAIEIARE MDIDKYDTIA CASGDGIPHE VINGLYQRPD 350
    HVKAFNNIAI TEIPCGSGNA MSVSCHWTNN PSYSTLCLIK SIETRIDLMC 400
    CSQPSYAREH PKLSFLSQTY GLIAETDINT EFIRWMGPAR FELGVAFNII 450
    QKKKYPCEIY VKYAAKSKNE LKNHYLEHKN KGSLEFQHIT MNKDNEDCDN 500
    YNYENEYETE NEDEDEDADA DDEDSHLISR DLADSSADQI KEEDFKIKYP 550
    LDEGIPSDWE RLDPNISNNL GIFYTGKMPY VAADTKFFPA ALPSDGTMDM 600
    VITDARTSLT RMAPILLGLD KGSHVLQPEV LHSKILAYKI IPKLGNGLFS 650
    VDGEKFPLEP LQVEIMPRLC KTLLRNGRYV DTDFDSM 687
    Length:687
    Mass (Da):77,566
    Last modified:November 1, 1996 - v1
    Checksum:i205C32F3570042FA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U17244 Genomic DNA. Translation: AAB67377.1.
    BK006945 Genomic DNA. Translation: DAA09573.1.
    PIRiS51398.
    RefSeqiNP_013361.1. NM_001182147.1.

    Genome annotation databases

    EnsemblFungiiYLR260W; YLR260W; YLR260W.
    GeneIDi850964.
    KEGGisce:YLR260W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U17244 Genomic DNA. Translation: AAB67377.1 .
    BK006945 Genomic DNA. Translation: DAA09573.1 .
    PIRi S51398.
    RefSeqi NP_013361.1. NM_001182147.1.

    3D structure databases

    ProteinModelPortali Q06147.
    SMRi Q06147. Positions 271-455, 550-667.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31528. 134 interactions.
    IntActi Q06147. 1 interaction.
    MINTi MINT-2784711.
    STRINGi 4932.YLR260W.

    Proteomic databases

    MaxQBi Q06147.
    PaxDbi Q06147.
    PeptideAtlasi Q06147.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YLR260W ; YLR260W ; YLR260W .
    GeneIDi 850964.
    KEGGi sce:YLR260W.

    Organism-specific databases

    CYGDi YLR260w.
    SGDi S000004250. LCB5.

    Phylogenomic databases

    eggNOGi COG1597.
    GeneTreei ENSGT00690000101761.
    HOGENOMi HOG000207396.
    KOi K04718.
    OMAi ISRTSFQ.
    OrthoDBi EOG7KH9TK.

    Enzyme and pathway databases

    BioCyci MetaCyc:YLR260W-MONOMER.
    YEAST:YLR260W-MONOMER.
    Reactomei REACT_188975. Glycosphingolipid metabolism.

    Miscellaneous databases

    NextBioi 967451.

    Gene expression databases

    Genevestigatori Q06147.

    Family and domain databases

    InterProi IPR016064. ATP-NAD_kinase_PpnK-typ.
    IPR001206. Diacylglycerol_kinase_cat_dom.
    [Graphical view ]
    Pfami PF00781. DAGK_cat. 1 hit.
    [Graphical view ]
    SMARTi SM00046. DAGKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF111331. SSF111331. 2 hits.
    PROSITEi PS50146. DAGK. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
      Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
      , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
      Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "The LCB4 (YOR171c) and LCB5 (YLR260w) genes of Saccharomyces encode sphingoid long chain base kinases."
      Nagiec M.M., Skrzypek M.S., Nagiec E.E., Lester R.L., Dickson R.C.
      J. Biol. Chem. 273:19437-19442(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
    4. "Accumulation of phosphorylated sphingoid long chain bases results in cell growth inhibition in Saccharomyces cerevisiae."
      Kim S., Fyrst H., Saba J.D.
      Genetics 156:1519-1529(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. "Role for de novo sphingoid base biosynthesis in the heat-induced transient cell cycle arrest of Saccharomyces cerevisiae."
      Jenkins G.M., Hannun Y.A.
      J. Biol. Chem. 276:8574-8581(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "Calcium influx and signaling in yeast stimulated by intracellular sphingosine 1-phosphate accumulation."
      Birchwood C.J., Saba J.D., Dickson R.C., Cunningham K.W.
      J. Biol. Chem. 276:11712-11718(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "Lcb4p is a key regulator of ceramide synthesis from exogenous long chain sphingoid base in Saccharomyces cerevisiae."
      Funato K., Lombardi R., Vallee B., Riezman H.
      J. Biol. Chem. 278:7325-7334(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiLCB5_YEAST
    AccessioniPrimary (citable) accession number: Q06147
    Secondary accession number(s): D6VYQ7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 31, 2006
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 105 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1760 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XII
      Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

    External Data

    Dasty 3