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Protein

Mitochondrial dicarboxylate transporter

Gene

DIC1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Mitochondrial dicarboxylic transporter catalyzing the exchange of dicarboxylic acids like malate and succinate for inorganic phosphate. Required for growth on ethanol and acetate.4 Publications

Kineticsi

  1. KM=1.55 mM for malonate3 Publications
  1. Vmax=3 µmol/min/mg enzyme3 Publications

GO - Molecular functioni

GO - Biological processi

  • dicarboxylic acid transport Source: SGD
  • phosphate ion transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Antiport, Phosphate transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-32424-MONOMER.
ReactomeiR-SCE-1614517. Sulfide oxidation to sulfate.
R-SCE-167826. The fatty acid cycling model.
R-SCE-167827. The proton buffering model.
R-SCE-428643. Organic anion transporters.
R-SCE-70263. Gluconeogenesis.
R-SCE-70326. Glucose metabolism.
SABIO-RKQ06143.

Protein family/group databases

TCDBi2.A.29.2.3. the mitochondrial carrier (mc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial dicarboxylate transporter
Alternative name(s):
DTP
Dicarboxylate carrier 1
Gene namesi
Name:DIC1
Ordered Locus Names:YLR348C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR348C.
SGDiS000004340. DIC1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei17 – 37Helical; Name=1Sequence analysisAdd BLAST21
Transmembranei58 – 76Helical; Name=2Sequence analysisAdd BLAST19
Transmembranei105 – 126Helical; Name=3Sequence analysisAdd BLAST22
Transmembranei170 – 189Helical; Name=4Sequence analysisAdd BLAST20
Transmembranei211 – 231Helical; Name=5Sequence analysisAdd BLAST21
Transmembranei265 – 283Helical; Name=6Sequence analysisAdd BLAST19

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002330051 – 298Mitochondrial dicarboxylate transporterAdd BLAST298

Proteomic databases

MaxQBiQ06143.
PRIDEiQ06143.

Interactioni

Subunit structurei

Homodimer. Binds to the TIM22 translocation complex during import.1 Publication

Protein-protein interaction databases

BioGridi31611. 31 interactors.
DIPiDIP-8816N.
IntActiQ06143. 1 interactor.
MINTiMINT-4496018.

Structurei

3D structure databases

ProteinModelPortaliQ06143.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati11 – 95Solcar 1Add BLAST85
Repeati103 – 195Solcar 2Add BLAST93
Repeati205 – 289Solcar 3Add BLAST85

Domaini

The C-terminal Solcar repeat is required for association with the TIM22 translocation complex during import.1 Publication

Sequence similaritiesi

Contains 3 Solcar repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00760000119170.
HOGENOMiHOG000165139.
InParanoidiQ06143.
KOiK13577.
OMAiTFGHVIK.
OrthoDBiEOG092C44NO.

Family and domain databases

Gene3Di1.50.40.10. 1 hit.
InterProiIPR018108. Mitochondrial_sb/sol_carrier.
IPR023395. Mt_carrier_dom.
[Graphical view]
PfamiPF00153. Mito_carr. 3 hits.
[Graphical view]
SUPFAMiSSF103506. SSF103506. 1 hit.
PROSITEiPS50920. SOLCAR. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q06143-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTNAKESAG KNIKYPWWYG GAAGIFATMV THPLDLAKVR LQAAPMPKPT
60 70 80 90 100
LFRMLESILA NEGVVGLYSG LSAAVLRQCT YTTVRFGAYD LLKENVIPRE
110 120 130 140 150
QLTNMAYLLP CSMFSGAIGG LAGNFADVVN IRMQNDSALE AAKRRNYKNA
160 170 180 190 200
IDGVYKIYRY EGGLKTLFTG WKPNMVRGIL MTASQVVTYD VFKNYLVTKL
210 220 230 240 250
DFDASKNYTH LTASLLAGLV ATTVCSPADV MKTRIMNGSG DHQPALKILA
260 270 280 290
DAVRKEGPSF MFRGWLPSFT RLGPFTMLIF FAIEQLKKHR VGMPKEDK
Length:298
Mass (Da):32,992
Last modified:November 1, 1996 - v1
Checksum:iC66A1F963450453D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U79459 Genomic DNA. Translation: AAB71336.1.
U19028 Genomic DNA. Translation: AAB67266.1.
BK006945 Genomic DNA. Translation: DAA09653.1.
PIRiS51351.
RefSeqiNP_013452.1. NM_001182237.1.

Genome annotation databases

EnsemblFungiiYLR348C; YLR348C; YLR348C.
GeneIDi851063.
KEGGisce:YLR348C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U79459 Genomic DNA. Translation: AAB71336.1.
U19028 Genomic DNA. Translation: AAB67266.1.
BK006945 Genomic DNA. Translation: DAA09653.1.
PIRiS51351.
RefSeqiNP_013452.1. NM_001182237.1.

3D structure databases

ProteinModelPortaliQ06143.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31611. 31 interactors.
DIPiDIP-8816N.
IntActiQ06143. 1 interactor.
MINTiMINT-4496018.

Protein family/group databases

TCDBi2.A.29.2.3. the mitochondrial carrier (mc) family.

Proteomic databases

MaxQBiQ06143.
PRIDEiQ06143.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR348C; YLR348C; YLR348C.
GeneIDi851063.
KEGGisce:YLR348C.

Organism-specific databases

EuPathDBiFungiDB:YLR348C.
SGDiS000004340. DIC1.

Phylogenomic databases

GeneTreeiENSGT00760000119170.
HOGENOMiHOG000165139.
InParanoidiQ06143.
KOiK13577.
OMAiTFGHVIK.
OrthoDBiEOG092C44NO.

Enzyme and pathway databases

BioCyciYEAST:G3O-32424-MONOMER.
ReactomeiR-SCE-1614517. Sulfide oxidation to sulfate.
R-SCE-167826. The fatty acid cycling model.
R-SCE-167827. The proton buffering model.
R-SCE-428643. Organic anion transporters.
R-SCE-70263. Gluconeogenesis.
R-SCE-70326. Glucose metabolism.
SABIO-RKQ06143.

Miscellaneous databases

PROiQ06143.

Family and domain databases

Gene3Di1.50.40.10. 1 hit.
InterProiIPR018108. Mitochondrial_sb/sol_carrier.
IPR023395. Mt_carrier_dom.
[Graphical view]
PfamiPF00153. Mito_carr. 3 hits.
[Graphical view]
SUPFAMiSSF103506. SSF103506. 1 hit.
PROSITEiPS50920. SOLCAR. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDIC1_YEAST
AccessioniPrimary (citable) accession number: Q06143
Secondary accession number(s): D6VYY7, P87332
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 18, 2006
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3390 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.