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Protein

Importin subunit beta-1

Gene

KAP95

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Importin beta subunit that functions in nuclear protein import through association with the importin alpha subunit, which binds to the classical nuclear localization signal (cNLS) in cargo substrates (PubMed:7622450). Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by importin beta through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism (PubMed:8521485). At the nucleoplasmic side of the NPC, GTP-Ran binds to importin beta and the three components separate, leading to release of the cargo (PubMed:15864302). Importin alpha and beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin beta. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (PubMed:11423015). Mediates the nuclear import of histones H2A and H2B (PubMed:11309407).1 Publication4 Publications

GO - Molecular functioni

  • nuclear localization sequence binding Source: GO_Central
  • protein transporter activity Source: SGD
  • Ran guanyl-nucleotide exchange factor activity Source: SGD

GO - Biological processi

  • mRNA transport Source: UniProtKB-KW
  • NLS-bearing protein import into nucleus Source: GO_Central
  • nuclear pore complex assembly Source: SGD
  • phosphatidylcholine biosynthetic process Source: SGD
  • protein import into nucleus Source: SGD
  • protein import into nucleus, docking Source: GO_Central
  • protein import into nucleus, translocation Source: GO_Central
  • protein targeting to membrane Source: SGD
  • regulation of protein desumoylation Source: SGD
  • ribosomal protein import into nucleus Source: GO_Central
Complete GO annotation...

Keywords - Biological processi

mRNA transport, Protein transport, Translocation, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-32423-MONOMER.
ReactomeiR-SCE-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Importin subunit beta-11 Publication
Alternative name(s):
Importin-95
Karyopherin subunit beta-11 Publication
Karyopherin-951 Publication
Gene namesi
Name:KAP951 Publication
Ordered Locus Names:YLR347CImported
ORF Names:L8300.15
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR347C.
SGDiS000004339. KAP95.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • nuclear membrane Source: GO_Central
  • nuclear periphery Source: GO_Central
  • nuclear pore Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nuclear pore complex, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001207642 – 861Importin subunit beta-1Add BLAST860

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei836PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ06142.
PRIDEiQ06142.

PTM databases

iPTMnetiQ06142.

Interactioni

Subunit structurei

Forms a complex with the importin alpha subunit (SRP1/KAP60) (PubMed:7622450). Interacts with Ran (GSP1); interacts specifically with the GTP-bound form of Ran (GTP-Ran), protecting it from GTP hydrolysis and nucleotide exchange (PubMed:8621381, PubMed:9321403). Interacts with nucleoporin NUP1 (PubMed:8521485, PubMed:15878174).5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
NSP1P149073EBI-9145,EBI-12265
NUP1P206766EBI-9145,EBI-12392
NUP100Q026294EBI-9145,EBI-11698
NUP116Q026304EBI-9145,EBI-11703
NUP159P404773EBI-9145,EBI-11747
NUP2P324995EBI-9145,EBI-12401
NUP42P496862EBI-9145,EBI-12310
NUP49Q021992EBI-9145,EBI-12315
NUP57P488372EBI-9145,EBI-12324
NUP60P397054EBI-9145,EBI-20731
SRP1Q028215EBI-9145,EBI-1797

Protein-protein interaction databases

BioGridi31610. 139 interactors.
DIPiDIP-2357N.
IntActiQ06142. 68 interactors.
MINTiMINT-476645.

Structurei

Secondary structure

1861
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 15Combined sources13
Helixi19 – 35Combined sources17
Helixi37 – 48Combined sources12
Helixi55 – 66Combined sources12
Turni67 – 69Combined sources3
Helixi74 – 87Combined sources14
Helixi90 – 104Combined sources15
Helixi109 – 126Combined sources18
Helixi127 – 129Combined sources3
Helixi134 – 142Combined sources9
Helixi149 – 164Combined sources16
Beta strandi166 – 169Combined sources4
Helixi174 – 176Combined sources3
Helixi177 – 188Combined sources12
Helixi195 – 208Combined sources14
Helixi209 – 211Combined sources3
Helixi213 – 216Combined sources4
Helixi219 – 233Combined sources15
Helixi238 – 255Combined sources18
Helixi256 – 258Combined sources3
Helixi260 – 265Combined sources6
Helixi267 – 274Combined sources8
Helixi280 – 306Combined sources27
Helixi317 – 332Combined sources16
Helixi346 – 362Combined sources17
Helixi363 – 366Combined sources4
Helixi367 – 377Combined sources11
Helixi383 – 395Combined sources13
Beta strandi397 – 400Combined sources4
Helixi402 – 418Combined sources17
Helixi419 – 421Combined sources3
Helixi425 – 442Combined sources18
Helixi443 – 445Combined sources3
Turni448 – 451Combined sources4
Helixi452 – 463Combined sources12
Helixi467 – 484Combined sources18
Beta strandi485 – 489Combined sources5
Helixi491 – 495Combined sources5
Helixi496 – 507Combined sources12
Beta strandi509 – 511Combined sources3
Helixi513 – 515Combined sources3
Helixi516 – 530Combined sources15
Helixi533 – 535Combined sources3
Helixi536 – 553Combined sources18
Helixi558 – 560Combined sources3
Helixi563 – 586Combined sources24
Helixi588 – 590Combined sources3
Helixi592 – 594Combined sources3
Helixi595 – 607Combined sources13
Beta strandi608 – 610Combined sources3
Helixi611 – 613Combined sources3
Helixi615 – 629Combined sources15
Helixi630 – 633Combined sources4
Helixi634 – 649Combined sources16
Helixi654 – 669Combined sources16
Helixi671 – 674Combined sources4
Helixi675 – 690Combined sources16
Helixi698 – 713Combined sources16
Helixi714 – 717Combined sources4
Helixi718 – 732Combined sources15
Beta strandi737 – 740Combined sources4
Helixi741 – 764Combined sources24
Turni765 – 767Combined sources3
Helixi769 – 772Combined sources4
Helixi773 – 775Combined sources3
Helixi776 – 788Combined sources13
Helixi790 – 793Combined sources4
Helixi796 – 812Combined sources17
Beta strandi816 – 818Combined sources3
Helixi819 – 821Combined sources3
Helixi825 – 836Combined sources12
Beta strandi838 – 840Combined sources3
Helixi842 – 859Combined sources18

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2BKUX-ray2.70B/D1-861[»]
2BPTX-ray1.99A1-861[»]
3EA5X-ray2.50B/D1-861[»]
3ND2X-ray2.40A1-861[»]
ProteinModelPortaliQ06142.
SMRiQ06142.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ06142.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati3 – 35HEAT 11 PublicationAdd BLAST33
Domaini25 – 106Importin N-terminalPROSITE-ProRule annotationAdd BLAST82
Repeati37 – 66HEAT 21 PublicationAdd BLAST30
Repeati90 – 129HEAT 31 PublicationAdd BLAST40
Repeati134 – 164HEAT 41 PublicationAdd BLAST31
Repeati177 – 208HEAT 51 PublicationAdd BLAST32
Repeati219 – 255HEAT 61 PublicationAdd BLAST37
Repeati260 – 306HEAT 71 PublicationAdd BLAST47
Repeati317 – 362HEAT 81 PublicationAdd BLAST46
Repeati367 – 395HEAT 91 PublicationAdd BLAST29
Repeati402 – 442HEAT 101 PublicationAdd BLAST41
Repeati452 – 484HEAT 111 PublicationAdd BLAST33
Repeati496 – 530HEAT 121 PublicationAdd BLAST35
Repeati536 – 586HEAT 131 PublicationAdd BLAST51
Repeati592 – 629HEAT 141 PublicationAdd BLAST38
Repeati634 – 669HEAT 151 PublicationAdd BLAST36
Repeati675 – 713HEAT 161 PublicationAdd BLAST39
Repeati718 – 764HEAT 171 PublicationAdd BLAST47
Repeati773 – 812HEAT 181 PublicationAdd BLAST40
Repeati819 – 859HEAT 191 PublicationAdd BLAST41

Sequence similaritiesi

Contains 19 HEAT repeats.1 Publication
Contains 1 importin N-terminal domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00550000074898.
HOGENOMiHOG000204108.
InParanoidiQ06142.
KOiK14293.
OMAiEFNARAS.
OrthoDBiEOG092C12XO.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000225. Armadillo.
IPR021133. HEAT_type_2.
IPR001494. Importin-beta_N.
[Graphical view]
PfamiPF03810. IBN_N. 1 hit.
[Graphical view]
SMARTiSM00185. ARM. 2 hits.
SM00913. IBN_N. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS50077. HEAT_REPEAT. 1 hit.
PS50166. IMPORTIN_B_NT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q06142-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTAEFAQLL ENSILSPDQN IRLTSETQLK KLSNDNFLQF AGLSSQVLID
60 70 80 90 100
ENTKLEGRIL AALTLKNELV SKDSVKTQQF AQRWITQVSP EAKNQIKTNA
110 120 130 140 150
LTALVSIEPR IANAAAQLIA AIADIELPHG AWPELMKIMV DNTGAEQPEN
160 170 180 190 200
VKRASLLALG YMCESADPQS QALVSSSNNI LIAIVQGAQS TETSKAVRLA
210 220 230 240 250
ALNALADSLI FIKNNMEREG ERNYLMQVVC EATQAEDIEV QAAAFGCLCK
260 270 280 290 300
IMSLYYTFMK PYMEQALYAL TIATMKSPND KVASMTVEFW STICEEEIDI
310 320 330 340 350
AYELAQFPQS PLQSYNFALS SIKDVVPNLL NLLTRQNEDP EDDDWNVSMS
360 370 380 390 400
AGACLQLFAQ NCGNHILEPV LEFVEQNITA DNWRNREAAV MAFGSIMDGP
410 420 430 440 450
DKVQRTYYVH QALPSILNLM NDQSLQVKET TAWCIGRIAD SVAESIDPQQ
460 470 480 490 500
HLPGVVQACL IGLQDHPKVA TNCSWTIINL VEQLAEATPS PIYNFYPALV
510 520 530 540 550
DGLIGAANRI DNEFNARASA FSALTTMVEY ATDTVAETSA SISTFVMDKL
560 570 580 590 600
GQTMSVDENQ LTLEDAQSLQ ELQSNILTVL AAVIRKSPSS VEPVADMLMG
610 620 630 640 650
LFFRLLEKKD SAFIEDDVFY AISALAASLG KGFEKYLETF SPYLLKALNQ
660 670 680 690 700
VDSPVSITAV GFIADISNSL EEDFRRYSDA MMNVLAQMIS NPNARRELKP
710 720 730 740 750
AVLSVFGDIA SNIGADFIPY LNDIMALCVA AQNTKPENGT LEALDYQIKV
760 770 780 790 800
LEAVLDAYVG IVAGLHDKPE ALFPYVGTIF QFIAQVAEDP QLYSEDATSR
810 820 830 840 850
AAVGLIGDIA AMFPDGSIKQ FYGQDWVIDY IKRTRSGQLF SQATKDTARW
860
AREQQKRQLS L
Length:861
Mass (Da):94,776
Last modified:November 1, 1996 - v1
Checksum:iED530F5313E75B06
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U19028 Genomic DNA. Translation: AAB67265.1.
BK006945 Genomic DNA. Translation: DAA09652.1.
PIRiS51350.
RefSeqiNP_013451.1. NM_001182236.1.

Genome annotation databases

EnsemblFungiiYLR347C; YLR347C; YLR347C.
GeneIDi851061.
KEGGisce:YLR347C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U19028 Genomic DNA. Translation: AAB67265.1.
BK006945 Genomic DNA. Translation: DAA09652.1.
PIRiS51350.
RefSeqiNP_013451.1. NM_001182236.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2BKUX-ray2.70B/D1-861[»]
2BPTX-ray1.99A1-861[»]
3EA5X-ray2.50B/D1-861[»]
3ND2X-ray2.40A1-861[»]
ProteinModelPortaliQ06142.
SMRiQ06142.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31610. 139 interactors.
DIPiDIP-2357N.
IntActiQ06142. 68 interactors.
MINTiMINT-476645.

PTM databases

iPTMnetiQ06142.

Proteomic databases

MaxQBiQ06142.
PRIDEiQ06142.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR347C; YLR347C; YLR347C.
GeneIDi851061.
KEGGisce:YLR347C.

Organism-specific databases

EuPathDBiFungiDB:YLR347C.
SGDiS000004339. KAP95.

Phylogenomic databases

GeneTreeiENSGT00550000074898.
HOGENOMiHOG000204108.
InParanoidiQ06142.
KOiK14293.
OMAiEFNARAS.
OrthoDBiEOG092C12XO.

Enzyme and pathway databases

BioCyciYEAST:G3O-32423-MONOMER.
ReactomeiR-SCE-6798695. Neutrophil degranulation.

Miscellaneous databases

EvolutionaryTraceiQ06142.
PROiQ06142.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000225. Armadillo.
IPR021133. HEAT_type_2.
IPR001494. Importin-beta_N.
[Graphical view]
PfamiPF03810. IBN_N. 1 hit.
[Graphical view]
SMARTiSM00185. ARM. 2 hits.
SM00913. IBN_N. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS50077. HEAT_REPEAT. 1 hit.
PS50166. IMPORTIN_B_NT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIMB1_YEAST
AccessioniPrimary (citable) accession number: Q06142
Secondary accession number(s): D6VYY6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 157 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Binds to nucleoporin FxFG but not GLFG repeat regions. Ran-GTP can disrupt the importin alpha/beta heterodimer by binding to the beta subunit and releases both subunits from the docking site.1 Publication
The stoichiometric complex between importin beta and Ran-GTP renders the latter inaccessible to Ran-specific GTPase activating protein (Ran-GAP) thereby inhibiting GTP hydrolysis stimulated by Ran-GAP.1 Publication
Present with 51700 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.