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Reviewed, UniProtKB/Swiss-Prot Q06142 (IMB1_YEAST)

Last modified February 9, 2010. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Importin subunit beta-1
Alternative name(s):
    Karyopherin subunit beta-1
    Karyopherin-95
    Importin-95
Gene names
Name: KAP95
Ordered Locus Names: YLR347C
ORF Names: L8300.15
OrganismSaccharomyces cerevisiae (Baker's yeast) [Complete proteome]
Taxonomic identifier4932 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length861 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Required for nuclear protein import and mediates docking of import substrate to distinct nucleoporins. Serves a receptor for nuclear localization signals. Mediates the nuclear import of histones H2A and H2B. Ref.5

Subunit structure

Forms a complex with importin alpha subunit.

Subcellular location

Cytoplasm By similarity. Nucleus envelope By similarity.

Miscellaneous

Binds to nucleoporin FxFG but not GLFG repeat regions. Ran-GTP can disrupt the karyopherin heterodimer by binding to the beta subunit and releases both subunits from the docking site.

The stoichiometric complex between the beta subunit and Ran-GTP renders the latter inaccessible to Ran-specific GTPase activating protein (Ran-GAP) thereby inhibiting GTP hydrolysis stimulated by Ran-GAP.

Present with 51700 molecules/cell in log phase SD medium. Ref.6

Sequence similarities

Belongs to the importin beta family.

Contains 8 HEAT repeats.

Contains 1 importin N-terminal domain.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 861861Importin subunit beta-1
PRO_0000120764

Regions

Domain25 – 10682Importin N-terminal
Repeat130 – 16839HEAT 1
Repeat220 – 25738HEAT 2
Repeat320 – 36445HEAT 3
Repeat366 – 40237HEAT 4
Repeat407 – 44438HEAT 5
Repeat571 – 60838HEAT 6
Repeat635 – 67238HEAT 7
Repeat676 – 71540HEAT 8
Region332 – 34514IAB
Region337 – 42286Ran-GTP binding By similarity

Amino acid modifications

Modified residue8361Phosphoserine Ref.7

Secondary structure

1 .................................................................................................................. 861
Helix Strand Turn

Details...

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Sequences

Sequence LengthMass (Da)Tools
Q06142-1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: ED530F5313E75B06

FASTA86194,776
        10         20         30         40         50         60 
MSTAEFAQLL ENSILSPDQN IRLTSETQLK KLSNDNFLQF AGLSSQVLID ENTKLEGRIL 

        70         80         90        100        110        120 
AALTLKNELV SKDSVKTQQF AQRWITQVSP EAKNQIKTNA LTALVSIEPR IANAAAQLIA 

       130        140        150        160        170        180 
AIADIELPHG AWPELMKIMV DNTGAEQPEN VKRASLLALG YMCESADPQS QALVSSSNNI 

       190        200        210        220        230        240 
LIAIVQGAQS TETSKAVRLA ALNALADSLI FIKNNMEREG ERNYLMQVVC EATQAEDIEV 

       250        260        270        280        290        300 
QAAAFGCLCK IMSLYYTFMK PYMEQALYAL TIATMKSPND KVASMTVEFW STICEEEIDI 

       310        320        330        340        350        360 
AYELAQFPQS PLQSYNFALS SIKDVVPNLL NLLTRQNEDP EDDDWNVSMS AGACLQLFAQ 

       370        380        390        400        410        420 
NCGNHILEPV LEFVEQNITA DNWRNREAAV MAFGSIMDGP DKVQRTYYVH QALPSILNLM 

       430        440        450        460        470        480 
NDQSLQVKET TAWCIGRIAD SVAESIDPQQ HLPGVVQACL IGLQDHPKVA TNCSWTIINL 

       490        500        510        520        530        540 
VEQLAEATPS PIYNFYPALV DGLIGAANRI DNEFNARASA FSALTTMVEY ATDTVAETSA 

       550        560        570        580        590        600 
SISTFVMDKL GQTMSVDENQ LTLEDAQSLQ ELQSNILTVL AAVIRKSPSS VEPVADMLMG 

       610        620        630        640        650        660 
LFFRLLEKKD SAFIEDDVFY AISALAASLG KGFEKYLETF SPYLLKALNQ VDSPVSITAV 

       670        680        690        700        710        720 
GFIADISNSL EEDFRRYSDA MMNVLAQMIS NPNARRELKP AVLSVFGDIA SNIGADFIPY 

       730        740        750        760        770        780 
LNDIMALCVA AQNTKPENGT LEALDYQIKV LEAVLDAYVG IVAGLHDKPE ALFPYVGTIF 

       790        800        810        820        830        840 
QFIAQVAEDP QLYSEDATSR AAVGLIGDIA AMFPDGSIKQ FYGQDWVIDY IKRTRSGQLF 

       850        860 
SQATKDTARW AREQQKRQLS L

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References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. expand/collapse author list , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
Nature 387:87-90(1997) [PubMed: 9169871] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[2]"Identification of a yeast karyopherin heterodimer that targets import substrate to mammalian nuclear pore complexes."
Enenkel C., Blobel G., Rexach M.
J. Biol. Chem. 270:16499-16502(1995) [PubMed: 7622450] [Abstract]
Cited for: PROTEIN SEQUENCE OF 819-846, IDENTIFICATION.
[3]"The nuclear transport factor karyopherin beta binds stoichiometrically to Ran-GTP and inhibits the Ran GTPase activating protein."
Floer M., Blobel G.
J. Biol. Chem. 271:5313-5316(1996) [PubMed: 8621381] [Abstract]
Cited for: RAN-GTP BINDING.
[4]"Protein import into nuclei: association and dissociation reactions involving transport substrate, transport factors, and nucleoporins."
Rexach M., Blobel G.
Cell 83:683-692(1995) [PubMed: 8521485] [Abstract]
Cited for: NUCLEOPORIN REPEAT BINDING REQUIREMENT.
[5]"Nuclear import of histone H2A and H2B is mediated by a network of karyopherins."
Mosammaparast N., Jackson K.R., Guo Y., Brame C.J., Shabanowitz J., Hunt D.F., Pemberton L.F.
J. Cell Biol. 153:251-262(2001) [PubMed: 11309407] [Abstract]
Cited for: FUNCTION IN HISTONE H2A/H2B IMPORT.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-836, MASS SPECTROMETRY.
+Additional computationally mapped references.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U19028 Genomic DNA. Translation: AAB67265.1.
PIRS51350.
RefSeqNP_013451.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2BKUX-ray2.70B/D1-861[»]
2BPTX-ray1.99A1-861[»]
3EA5X-ray2.50B/D1-861[»]
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-2357N.
IntActQ06142. 92 interactions.
STRINGQ06142.

Proteomic databases

PeptideAtlasQ06142.
PRIDEQ06142.

Genome annotation databases

EnsemblYLR347C; YLR347C; YLR347C; Saccharomyces cerevisiae. [Genome view]
GeneID851061.
KEGGsce:YLR347C.
NMPDRfig|4932.3.peg.4472.

Organism-specific databases

CYGDYLR347c.
SGDS000004339. KAP95.

Phylogenomic databases

eggNOGfuNOG04024.
HOGENOMHBG357195.
OMADTARWAR.
OrthoDBEOG9JT1NM.
PhylomeDBQ06142.

Gene expression databases

ArrayExpressQ06142.
GenevestigatorQ06142.
GermOnlineYLR347C. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000357. HEAT.
IPR021133. HEAT_type_2.
IPR001494. Importin-b_N.
[Graphical view]
Gene3DG3DSA:1.25.10.10. ARM-like. 1 hit.
PfamPF02985. HEAT. 1 hit.
PF03810. IBN_N. 1 hit.
[Graphical view]
PROSITEPS50077. HEAT_REPEAT. 1 hit.
PS50166. IMPORTIN_B_NT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio967690.

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Entry information

Entry nameIMB1_YEAST
AccessionPrimary (citable) accession number: Q06142
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: February 9, 2010
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

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Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents