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Protein

Importin subunit beta-1

Gene

KAP95

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Importin beta subunit that functions in nuclear protein import through association with the importin alpha subunit, which binds to the classical nuclear localization signal (cNLS) in cargo substrates (PubMed:7622450). Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by importin beta through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism (PubMed:8521485). At the nucleoplasmic side of the NPC, GTP-Ran binds to importin beta and the three components separate, leading to release of the cargo (PubMed:15864302). Importin alpha and beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin beta. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (PubMed:11423015). Mediates the nuclear import of histones H2A and H2B (PubMed:11309407).1 Publication4 Publications

GO - Molecular functioni

  • nuclear localization sequence binding Source: GO_Central
  • protein transporter activity Source: SGD
  • Ran guanyl-nucleotide exchange factor activity Source: SGD

GO - Biological processi

  • mRNA transport Source: UniProtKB-KW
  • NLS-bearing protein import into nucleus Source: GO_Central
  • nuclear pore complex assembly Source: SGD
  • phosphatidylcholine biosynthetic process Source: SGD
  • protein import into nucleus Source: SGD
  • protein import into nucleus, docking Source: GO_Central
  • protein import into nucleus, translocation Source: GO_Central
  • protein targeting to membrane Source: SGD
  • regulation of protein desumoylation Source: SGD
  • ribosomal protein import into nucleus Source: GO_Central
Complete GO annotation...

Keywords - Biological processi

mRNA transport, Protein transport, Translocation, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-32423-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Importin subunit beta-11 Publication
Alternative name(s):
Importin-95
Karyopherin subunit beta-11 Publication
Karyopherin-951 Publication
Gene namesi
Name:KAP951 Publication
Ordered Locus Names:YLR347CImported
ORF Names:L8300.15
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR347C.
SGDiS000004339. KAP95.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • nuclear membrane Source: GO_Central
  • nuclear periphery Source: GO_Central
  • nuclear pore Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nuclear pore complex, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources
Chaini2 – 861860Importin subunit beta-1PRO_0000120764Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineCombined sources
Modified residuei836 – 8361PhosphoserineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ06142.

PTM databases

iPTMnetiQ06142.

Interactioni

Subunit structurei

Forms a complex with the importin alpha subunit (SRP1/KAP60) (PubMed:7622450). Interacts with Ran (GSP1); interacts specifically with the GTP-bound form of Ran (GTP-Ran), protecting it from GTP hydrolysis and nucleotide exchange (PubMed:8621381, PubMed:9321403). Interacts with nucleoporin NUP1 (PubMed:8521485, PubMed:15878174).5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
NSP1P149073EBI-9145,EBI-12265
NUP1P206766EBI-9145,EBI-12392
NUP100Q026294EBI-9145,EBI-11698
NUP116Q026304EBI-9145,EBI-11703
NUP159P404773EBI-9145,EBI-11747
NUP2P324995EBI-9145,EBI-12401
NUP42P496862EBI-9145,EBI-12310
NUP49Q021992EBI-9145,EBI-12315
NUP57P488372EBI-9145,EBI-12324
NUP60P397054EBI-9145,EBI-20731
SRP1Q028215EBI-9145,EBI-1797

Protein-protein interaction databases

BioGridi31610. 139 interactions.
DIPiDIP-2357N.
IntActiQ06142. 68 interactions.
MINTiMINT-476645.

Structurei

Secondary structure

1
861
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 1513Combined sources
Helixi19 – 3517Combined sources
Helixi37 – 4812Combined sources
Helixi55 – 6612Combined sources
Turni67 – 693Combined sources
Helixi74 – 8714Combined sources
Helixi90 – 10415Combined sources
Helixi109 – 12618Combined sources
Helixi127 – 1293Combined sources
Helixi134 – 1429Combined sources
Helixi149 – 16416Combined sources
Beta strandi166 – 1694Combined sources
Helixi174 – 1763Combined sources
Helixi177 – 18812Combined sources
Helixi195 – 20814Combined sources
Helixi209 – 2113Combined sources
Helixi213 – 2164Combined sources
Helixi219 – 23315Combined sources
Helixi238 – 25518Combined sources
Helixi256 – 2583Combined sources
Helixi260 – 2656Combined sources
Helixi267 – 2748Combined sources
Helixi280 – 30627Combined sources
Helixi317 – 33216Combined sources
Helixi346 – 36217Combined sources
Helixi363 – 3664Combined sources
Helixi367 – 37711Combined sources
Helixi383 – 39513Combined sources
Beta strandi397 – 4004Combined sources
Helixi402 – 41817Combined sources
Helixi419 – 4213Combined sources
Helixi425 – 44218Combined sources
Helixi443 – 4453Combined sources
Turni448 – 4514Combined sources
Helixi452 – 46312Combined sources
Helixi467 – 48418Combined sources
Beta strandi485 – 4895Combined sources
Helixi491 – 4955Combined sources
Helixi496 – 50712Combined sources
Beta strandi509 – 5113Combined sources
Helixi513 – 5153Combined sources
Helixi516 – 53015Combined sources
Helixi533 – 5353Combined sources
Helixi536 – 55318Combined sources
Helixi558 – 5603Combined sources
Helixi563 – 58624Combined sources
Helixi588 – 5903Combined sources
Helixi592 – 5943Combined sources
Helixi595 – 60713Combined sources
Beta strandi608 – 6103Combined sources
Helixi611 – 6133Combined sources
Helixi615 – 62915Combined sources
Helixi630 – 6334Combined sources
Helixi634 – 64916Combined sources
Helixi654 – 66916Combined sources
Helixi671 – 6744Combined sources
Helixi675 – 69016Combined sources
Helixi698 – 71316Combined sources
Helixi714 – 7174Combined sources
Helixi718 – 73215Combined sources
Beta strandi737 – 7404Combined sources
Helixi741 – 76424Combined sources
Turni765 – 7673Combined sources
Helixi769 – 7724Combined sources
Helixi773 – 7753Combined sources
Helixi776 – 78813Combined sources
Helixi790 – 7934Combined sources
Helixi796 – 81217Combined sources
Beta strandi816 – 8183Combined sources
Helixi819 – 8213Combined sources
Helixi825 – 83612Combined sources
Beta strandi838 – 8403Combined sources
Helixi842 – 85918Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2BKUX-ray2.70B/D1-861[»]
2BPTX-ray1.99A1-861[»]
3EA5X-ray2.50B/D1-861[»]
3ND2X-ray2.40A1-861[»]
ProteinModelPortaliQ06142.
SMRiQ06142. Positions 1-861.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ06142.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati3 – 3533HEAT 11 PublicationAdd
BLAST
Domaini25 – 10682Importin N-terminalPROSITE-ProRule annotationAdd
BLAST
Repeati37 – 6630HEAT 21 PublicationAdd
BLAST
Repeati90 – 12940HEAT 31 PublicationAdd
BLAST
Repeati134 – 16431HEAT 41 PublicationAdd
BLAST
Repeati177 – 20832HEAT 51 PublicationAdd
BLAST
Repeati219 – 25537HEAT 61 PublicationAdd
BLAST
Repeati260 – 30647HEAT 71 PublicationAdd
BLAST
Repeati317 – 36246HEAT 81 PublicationAdd
BLAST
Repeati367 – 39529HEAT 91 PublicationAdd
BLAST
Repeati402 – 44241HEAT 101 PublicationAdd
BLAST
Repeati452 – 48433HEAT 111 PublicationAdd
BLAST
Repeati496 – 53035HEAT 121 PublicationAdd
BLAST
Repeati536 – 58651HEAT 131 PublicationAdd
BLAST
Repeati592 – 62938HEAT 141 PublicationAdd
BLAST
Repeati634 – 66936HEAT 151 PublicationAdd
BLAST
Repeati675 – 71339HEAT 161 PublicationAdd
BLAST
Repeati718 – 76447HEAT 171 PublicationAdd
BLAST
Repeati773 – 81240HEAT 181 PublicationAdd
BLAST
Repeati819 – 85941HEAT 191 PublicationAdd
BLAST

Sequence similaritiesi

Contains 19 HEAT repeats.1 Publication
Contains 1 importin N-terminal domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00550000074898.
HOGENOMiHOG000204108.
InParanoidiQ06142.
KOiK14293.
OMAiEFNARAS.
OrthoDBiEOG092C12XO.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000225. Armadillo.
IPR021133. HEAT_type_2.
IPR001494. Importin-beta_N.
[Graphical view]
PfamiPF03810. IBN_N. 1 hit.
[Graphical view]
SMARTiSM00185. ARM. 2 hits.
SM00913. IBN_N. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS50077. HEAT_REPEAT. 1 hit.
PS50166. IMPORTIN_B_NT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q06142-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTAEFAQLL ENSILSPDQN IRLTSETQLK KLSNDNFLQF AGLSSQVLID
60 70 80 90 100
ENTKLEGRIL AALTLKNELV SKDSVKTQQF AQRWITQVSP EAKNQIKTNA
110 120 130 140 150
LTALVSIEPR IANAAAQLIA AIADIELPHG AWPELMKIMV DNTGAEQPEN
160 170 180 190 200
VKRASLLALG YMCESADPQS QALVSSSNNI LIAIVQGAQS TETSKAVRLA
210 220 230 240 250
ALNALADSLI FIKNNMEREG ERNYLMQVVC EATQAEDIEV QAAAFGCLCK
260 270 280 290 300
IMSLYYTFMK PYMEQALYAL TIATMKSPND KVASMTVEFW STICEEEIDI
310 320 330 340 350
AYELAQFPQS PLQSYNFALS SIKDVVPNLL NLLTRQNEDP EDDDWNVSMS
360 370 380 390 400
AGACLQLFAQ NCGNHILEPV LEFVEQNITA DNWRNREAAV MAFGSIMDGP
410 420 430 440 450
DKVQRTYYVH QALPSILNLM NDQSLQVKET TAWCIGRIAD SVAESIDPQQ
460 470 480 490 500
HLPGVVQACL IGLQDHPKVA TNCSWTIINL VEQLAEATPS PIYNFYPALV
510 520 530 540 550
DGLIGAANRI DNEFNARASA FSALTTMVEY ATDTVAETSA SISTFVMDKL
560 570 580 590 600
GQTMSVDENQ LTLEDAQSLQ ELQSNILTVL AAVIRKSPSS VEPVADMLMG
610 620 630 640 650
LFFRLLEKKD SAFIEDDVFY AISALAASLG KGFEKYLETF SPYLLKALNQ
660 670 680 690 700
VDSPVSITAV GFIADISNSL EEDFRRYSDA MMNVLAQMIS NPNARRELKP
710 720 730 740 750
AVLSVFGDIA SNIGADFIPY LNDIMALCVA AQNTKPENGT LEALDYQIKV
760 770 780 790 800
LEAVLDAYVG IVAGLHDKPE ALFPYVGTIF QFIAQVAEDP QLYSEDATSR
810 820 830 840 850
AAVGLIGDIA AMFPDGSIKQ FYGQDWVIDY IKRTRSGQLF SQATKDTARW
860
AREQQKRQLS L
Length:861
Mass (Da):94,776
Last modified:November 1, 1996 - v1
Checksum:iED530F5313E75B06
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U19028 Genomic DNA. Translation: AAB67265.1.
BK006945 Genomic DNA. Translation: DAA09652.1.
PIRiS51350.
RefSeqiNP_013451.1. NM_001182236.1.

Genome annotation databases

EnsemblFungiiYLR347C; YLR347C; YLR347C.
GeneIDi851061.
KEGGisce:YLR347C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U19028 Genomic DNA. Translation: AAB67265.1.
BK006945 Genomic DNA. Translation: DAA09652.1.
PIRiS51350.
RefSeqiNP_013451.1. NM_001182236.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2BKUX-ray2.70B/D1-861[»]
2BPTX-ray1.99A1-861[»]
3EA5X-ray2.50B/D1-861[»]
3ND2X-ray2.40A1-861[»]
ProteinModelPortaliQ06142.
SMRiQ06142. Positions 1-861.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31610. 139 interactions.
DIPiDIP-2357N.
IntActiQ06142. 68 interactions.
MINTiMINT-476645.

PTM databases

iPTMnetiQ06142.

Proteomic databases

MaxQBiQ06142.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR347C; YLR347C; YLR347C.
GeneIDi851061.
KEGGisce:YLR347C.

Organism-specific databases

EuPathDBiFungiDB:YLR347C.
SGDiS000004339. KAP95.

Phylogenomic databases

GeneTreeiENSGT00550000074898.
HOGENOMiHOG000204108.
InParanoidiQ06142.
KOiK14293.
OMAiEFNARAS.
OrthoDBiEOG092C12XO.

Enzyme and pathway databases

BioCyciYEAST:G3O-32423-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ06142.
PROiQ06142.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000225. Armadillo.
IPR021133. HEAT_type_2.
IPR001494. Importin-beta_N.
[Graphical view]
PfamiPF03810. IBN_N. 1 hit.
[Graphical view]
SMARTiSM00185. ARM. 2 hits.
SM00913. IBN_N. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS50077. HEAT_REPEAT. 1 hit.
PS50166. IMPORTIN_B_NT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIMB1_YEAST
AccessioniPrimary (citable) accession number: Q06142
Secondary accession number(s): D6VYY6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: September 7, 2016
This is version 154 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Binds to nucleoporin FxFG but not GLFG repeat regions. Ran-GTP can disrupt the importin alpha/beta heterodimer by binding to the beta subunit and releases both subunits from the docking site.1 Publication
The stoichiometric complex between importin beta and Ran-GTP renders the latter inaccessible to Ran-specific GTPase activating protein (Ran-GAP) thereby inhibiting GTP hydrolysis stimulated by Ran-GAP.1 Publication
Present with 51700 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.