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Protein

Regenerating islet-derived protein 3-alpha

Gene

REG3A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Bactericidal C-type lectin which acts exclusively against Gram-positive bacteria and mediates bacterial killing by binding to surface-exposed carbohydrate moieties of peptidoglycan. Regulates keratinocyte proliferation and differentiation after skin injury via activation of EXTL3-PI3K-AKT signaling pathway.1 Publication

GO - Molecular functioni

  • carbohydrate binding Source: ProtInc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Antimicrobial

Keywords - Biological processi

Acute phase, Inflammatory response

Keywords - Ligandi

Lectin

Enzyme and pathway databases

BioCyciZFISH:ENSG00000172016-MONOMER.
ReactomeiR-HSA-6803157. Antimicrobial peptides.

Protein family/group databases

MEROPSiI63.002.
TCDBi1.C.111.1.2. the regiii (regiii) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Regenerating islet-derived protein 3-alpha
Short name:
REG-3-alpha
Alternative name(s):
Hepatointestinal pancreatic protein
Short name:
HIP/PAP
Human proislet peptide
Pancreatitis-associated protein 1
Regenerating islet-derived protein III-alpha
Short name:
Reg III-alpha
Cleaved into the following 2 chains:
Gene namesi
Name:REG3A
Synonyms:HIP, PAP, PAP1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:8601. REG3A.

Subcellular locationi

  • Secreted

  • Note: Found in the apical region of pancreatic acinar cells.

GO - Cellular componenti

  • cytoplasm Source: ProtInc
  • extracellular space Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi114E → Q: Reduces peptidoglycan binding and antibacterial activity. 1 Publication1
Mutagenesisi118E → Q: Reduces antibacterial activity but no effect on peptidoglycan binding. 1 Publication1

Organism-specific databases

DisGeNETi5068.
OpenTargetsiENSG00000172016.
PharmGKBiPA32931.

Polymorphism and mutation databases

BioMutaiREG3A.
DMDMi464341.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 26By similarityAdd BLAST26
ChainiPRO_000001742927 – 175Regenerating islet-derived protein 3-alpha 16.5 kDa formAdd BLAST149
PropeptideiPRO_000042274127 – 371 PublicationAdd BLAST11
ChainiPRO_000042274238 – 175Regenerating islet-derived protein 3-alpha 15 kDa formAdd BLAST138

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi40 ↔ 51PROSITE-ProRule annotation
Disulfide bondi68 ↔ 171PROSITE-ProRule annotation
Disulfide bondi146 ↔ 163PROSITE-ProRule annotation

Post-translational modificationi

Proteolytic processing by trypsin removes an inhibitory N-terminal propeptide and is essential for peptidoglycan binding and antibacterial activity.1 Publication

Keywords - PTMi

Disulfide bond

Proteomic databases

EPDiQ06141.
PaxDbiQ06141.
PeptideAtlasiQ06141.
PRIDEiQ06141.

Miscellaneous databases

PMAP-CutDBQ06141.

Expressioni

Tissue specificityi

Highly expressed in epidermal keratinocytes of psoriasis patients (at protein level). Constitutively expressed in intestine. Low expression is found in healthy pancreas. Overexpressed during the acute phase of pancreatitis and in some patients with chronic pancreatitis.2 Publications

Inductioni

Appears in pancreatic juice after induction of pancreatic inflammation.

Gene expression databases

BgeeiENSG00000172016.
CleanExiHS_REG3A.
ExpressionAtlasiQ06141. baseline and differential.
GenevisibleiQ06141. HS.

Organism-specific databases

HPAiHPA048334.

Interactioni

Subunit structurei

Interacts with EXTL3.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
MDFIQ997503EBI-10223932,EBI-724076

Protein-protein interaction databases

BioGridi111103. 6 interactors.
DIPiDIP-60688N.
IntActiQ06141. 1 interactor.
STRINGi9606.ENSP00000304311.

Structurei

Secondary structure

1175
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi44 – 47Combined sources4
Beta strandi50 – 59Combined sources10
Helixi61 – 68Combined sources8
Helixi82 – 92Combined sources11
Beta strandi100 – 107Combined sources8
Turni109 – 112Combined sources4
Beta strandi114 – 116Combined sources3
Turni123 – 125Combined sources3
Beta strandi133 – 135Combined sources3
Helixi137 – 139Combined sources3
Beta strandi140 – 142Combined sources3
Beta strandi145 – 150Combined sources6
Helixi151 – 153Combined sources3
Beta strandi157 – 161Combined sources5
Beta strandi167 – 173Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1UV0X-ray1.78A27-175[»]
2GO0NMR-A39-175[»]
4MTHX-ray1.47A38-175[»]
ProteinModelPortaliQ06141.
SMRiQ06141.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ06141.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini47 – 172C-type lectinPROSITE-ProRule annotationAdd BLAST126

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi114 – 116EPN3

Domaini

The EPN motif is essential for recognition of the peptidoglycan carbohydrate backbone and for efficient bacterial killing with Glu-114 playing a key role in peptidoglycan binding and bactericidal activity.

Sequence similaritiesi

Contains 1 C-type lectin domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG4297. Eukaryota.
ENOG410XPJ1. LUCA.
GeneTreeiENSGT00700000104249.
HOGENOMiHOG000010281.
HOVERGENiHBG004151.
InParanoidiQ06141.
OMAiVKLPYVC.
OrthoDBiEOG091G0MDY.
PhylomeDBiQ06141.

Family and domain databases

Gene3Di3.10.100.10. 1 hit.
InterProiIPR001304. C-type_lectin-like.
IPR016186. C-type_lectin-like/link.
IPR018378. C-type_lectin_CS.
IPR016187. CTDL_fold.
[Graphical view]
PfamiPF00059. Lectin_C. 1 hit.
[Graphical view]
SMARTiSM00034. CLECT. 1 hit.
[Graphical view]
SUPFAMiSSF56436. SSF56436. 1 hit.
PROSITEiPS00615. C_TYPE_LECTIN_1. 1 hit.
PS50041. C_TYPE_LECTIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q06141-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLPPMALPSV SWMLLSCLML LSQVQGEEPQ RELPSARIRC PKGSKAYGSH
60 70 80 90 100
CYALFLSPKS WTDADLACQK RPSGNLVSVL SGAEGSFVSS LVKSIGNSYS
110 120 130 140 150
YVWIGLHDPT QGTEPNGEGW EWSSSDVMNY FAWERNPSTI SSPGHCASLS
160 170
RSTAFLRWKD YNCNVRLPYV CKFTD
Length:175
Mass (Da):19,395
Last modified:February 1, 1994 - v1
Checksum:iC51149FAC22EB68C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti173 – 175FTD → VH in AAA36415 (PubMed:1469087).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13510 mRNA. Translation: BAA02728.1.
M84337 mRNA. Translation: AAA36415.1.
S51768 mRNA. Translation: AAB24642.1.
X68641 mRNA. Translation: CAA48605.1.
L15533 Genomic DNA. Translation: AAA60020.1.
BC036776 mRNA. Translation: AAH36776.1.
CCDSiCCDS1965.1.
PIRiA49616.
RefSeqiNP_002571.1. NM_002580.2.
NP_620354.1. NM_138937.2.
NP_620355.1. NM_138938.2.
UniGeneiHs.567312.

Genome annotation databases

EnsembliENST00000305165; ENSP00000304311; ENSG00000172016.
ENST00000393878; ENSP00000377456; ENSG00000172016.
ENST00000409839; ENSP00000386630; ENSG00000172016.
GeneIDi5068.
KEGGihsa:5068.

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - Glycan Binding

Pancreatitis-associated protein 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13510 mRNA. Translation: BAA02728.1.
M84337 mRNA. Translation: AAA36415.1.
S51768 mRNA. Translation: AAB24642.1.
X68641 mRNA. Translation: CAA48605.1.
L15533 Genomic DNA. Translation: AAA60020.1.
BC036776 mRNA. Translation: AAH36776.1.
CCDSiCCDS1965.1.
PIRiA49616.
RefSeqiNP_002571.1. NM_002580.2.
NP_620354.1. NM_138937.2.
NP_620355.1. NM_138938.2.
UniGeneiHs.567312.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1UV0X-ray1.78A27-175[»]
2GO0NMR-A39-175[»]
4MTHX-ray1.47A38-175[»]
ProteinModelPortaliQ06141.
SMRiQ06141.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111103. 6 interactors.
DIPiDIP-60688N.
IntActiQ06141. 1 interactor.
STRINGi9606.ENSP00000304311.

Protein family/group databases

MEROPSiI63.002.
TCDBi1.C.111.1.2. the regiii (regiii) family.

Polymorphism and mutation databases

BioMutaiREG3A.
DMDMi464341.

Proteomic databases

EPDiQ06141.
PaxDbiQ06141.
PeptideAtlasiQ06141.
PRIDEiQ06141.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000305165; ENSP00000304311; ENSG00000172016.
ENST00000393878; ENSP00000377456; ENSG00000172016.
ENST00000409839; ENSP00000386630; ENSG00000172016.
GeneIDi5068.
KEGGihsa:5068.

Organism-specific databases

CTDi5068.
DisGeNETi5068.
GeneCardsiREG3A.
HGNCiHGNC:8601. REG3A.
HPAiHPA048334.
MIMi167805. gene.
neXtProtiNX_Q06141.
OpenTargetsiENSG00000172016.
PharmGKBiPA32931.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4297. Eukaryota.
ENOG410XPJ1. LUCA.
GeneTreeiENSGT00700000104249.
HOGENOMiHOG000010281.
HOVERGENiHBG004151.
InParanoidiQ06141.
OMAiVKLPYVC.
OrthoDBiEOG091G0MDY.
PhylomeDBiQ06141.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000172016-MONOMER.
ReactomeiR-HSA-6803157. Antimicrobial peptides.

Miscellaneous databases

ChiTaRSiREG3A. human.
EvolutionaryTraceiQ06141.
GeneWikiiREG3A.
GenomeRNAii5068.
PMAP-CutDBQ06141.
PROiQ06141.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000172016.
CleanExiHS_REG3A.
ExpressionAtlasiQ06141. baseline and differential.
GenevisibleiQ06141. HS.

Family and domain databases

Gene3Di3.10.100.10. 1 hit.
InterProiIPR001304. C-type_lectin-like.
IPR016186. C-type_lectin-like/link.
IPR018378. C-type_lectin_CS.
IPR016187. CTDL_fold.
[Graphical view]
PfamiPF00059. Lectin_C. 1 hit.
[Graphical view]
SMARTiSM00034. CLECT. 1 hit.
[Graphical view]
SUPFAMiSSF56436. SSF56436. 1 hit.
PROSITEiPS00615. C_TYPE_LECTIN_1. 1 hit.
PS50041. C_TYPE_LECTIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiREG3A_HUMAN
AccessioniPrimary (citable) accession number: Q06141
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: November 30, 2016
This is version 150 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.