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Protein

Putative 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase YLR345W

Gene

YLR345W

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Synthesis and degradation of fructose 2,6-bisphosphate.By similarity

Catalytic activityi

Beta-D-fructose 2,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate.
ATP + D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei173 – 1731Proton donor/acceptorBy similarity
Binding sitei237 – 2371Fructose 6-phosphateBy similarity
Binding sitei298 – 2981Fructose 2,6-bisphosphateBy similarity
Sitei298 – 2981Transition state stabilizerBy similarity
Binding sitei433 – 4331Fructose 2,6-bisphosphateBy similarity
Sitei459 – 4591Transition state stabilizerBy similarity
Binding sitei464 – 4641Fructose 2,6-bisphosphateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi90 – 989ATPBy similarity
Nucleotide bindingi212 – 2176ATPBy similarity
Nucleotide bindingi415 – 4184ATPBy similarity
Nucleotide bindingi460 – 4645ATPBy similarity

GO - Molecular functioni

GO - Biological processi

  • carbohydrate phosphorylation Source: GOC
  • fructose 2,6-bisphosphate metabolic process Source: SGD
  • fructose metabolic process Source: InterPro
  • regulation of glycolytic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32421-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase YLR345W
Including the following 2 domains:
6-phosphofructo-2-kinase (EC:2.7.1.105)
Fructose-2,6-bisphosphatase (EC:3.1.3.46)
Gene namesi
Ordered Locus Names:YLR345W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR345W.
SGDiS000004337. YLR345W.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 509509Putative 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase YLR345WPRO_0000268185Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei6 – 61PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ06137.
PeptideAtlasiQ06137.

PTM databases

iPTMnetiQ06137.

Expressioni

Inductioni

Expression is under the control of the RFX1 transcription factor.1 Publication

Interactioni

Subunit structurei

Homodimer.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
FBP26P326043EBI-33827,EBI-6749
MTR3P482403EBI-33827,EBI-1749
SFH1Q061683EBI-33827,EBI-35813

Protein-protein interaction databases

BioGridi31608. 29 interactions.
DIPiDIP-1500N.
IntActiQ06137. 15 interactions.
MINTiMINT-398775.

Structurei

3D structure databases

ProteinModelPortaliQ06137.
SMRiQ06137. Positions 85-481.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni6 – 2912866-phosphofructo-2-kinaseBy similarityAdd
BLAST
Regioni292 – 466175Fructose-2,6-bisphosphataseBy similarityAdd
BLAST

Sequence similaritiesi

In the C-terminal section; belongs to the phosphoglycerate mutase family.Curated

Phylogenomic databases

GeneTreeiENSGT00550000075979.
HOGENOMiHOG000200602.
InParanoidiQ06137.
KOiK19030.
OMAiHEEYYET.
OrthoDBiEOG7B05NZ.

Family and domain databases

Gene3Di3.40.50.1240. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR003094. 6Pfruct_kin.
IPR013079. 6Phosfructo_kin.
IPR013078. His_Pase_superF_clade-1.
IPR029033. His_PPase_superfam.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR10606. PTHR10606. 2 hits.
PfamiPF01591. 6PF2K. 1 hit.
PF00300. His_Phos_1. 1 hit.
[Graphical view]
PRINTSiPR00991. 6PFRUCTKNASE.
SMARTiSM00855. PGAM. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF53254. SSF53254. 1 hit.

Sequencei

Sequence statusi: Complete.

Q06137-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPNVLSDDEE LLNGLGSEIM KPSRQGNHMA RTVKRWVNKE RATTADLKNV
60 70 80 90 100
NIDGVHGPVN TESYISPGQL YSTDSGNLFH AGRILVVLVG LPATSKTLLS
110 120 130 140 150
VAITRYTRWL GVRTKSFHFS EYKESAKNIP SDYFCVVPTS KEGVAFVEKL
160 170 180 190 200
RMQMLNDILA FFNDLSGQLA IYDALNIRKI DRKNLETTFS EIGVKVLFIE
210 220 230 240 250
SIVSDQEIMN RNIALALESN DYKGLSTDEA IDEYMRRLSV NEPYYEMMTH
260 270 280 290 300
DEELSYIKYI NLGKQIIVKD NIHGYLVNKI VFFLMNLRQK KGCVYFARCG
310 320 330 340 350
TSDKDNYIHD EELNEEGIHY SQVLKDFVLQ RIKQKRQAKK NSDSLVEVID
360 370 380 390 400
GSHDEDLKTS LIVWTGPRKR THDTALFFSK EGIKVQQRSE LRQLNPGSIA
410 420 430 440 450
DLTDQQIMDK FPSEYKESLK DPYHFRFPRA ESYHDLAVRM EPLLLEMEHT
460 470 480 490 500
SKDILIIAHE STLRVLYGYL MACTCVELPN LNFTRDKLVE ISFSPFCNTV

ELLNIPLTS
Length:509
Mass (Da):58,386
Last modified:November 1, 1996 - v1
Checksum:i29718CA8ECA8EB4E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U19028 Genomic DNA. Translation: AAB67253.1.
BK006945 Genomic DNA. Translation: DAA09650.1.
PIRiS51348.
RefSeqiNP_013449.1. NM_001182234.1.

Genome annotation databases

EnsemblFungiiYLR345W; YLR345W; YLR345W.
GeneIDi851059.
KEGGisce:YLR345W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U19028 Genomic DNA. Translation: AAB67253.1.
BK006945 Genomic DNA. Translation: DAA09650.1.
PIRiS51348.
RefSeqiNP_013449.1. NM_001182234.1.

3D structure databases

ProteinModelPortaliQ06137.
SMRiQ06137. Positions 85-481.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31608. 29 interactions.
DIPiDIP-1500N.
IntActiQ06137. 15 interactions.
MINTiMINT-398775.

PTM databases

iPTMnetiQ06137.

Proteomic databases

MaxQBiQ06137.
PeptideAtlasiQ06137.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR345W; YLR345W; YLR345W.
GeneIDi851059.
KEGGisce:YLR345W.

Organism-specific databases

EuPathDBiFungiDB:YLR345W.
SGDiS000004337. YLR345W.

Phylogenomic databases

GeneTreeiENSGT00550000075979.
HOGENOMiHOG000200602.
InParanoidiQ06137.
KOiK19030.
OMAiHEEYYET.
OrthoDBiEOG7B05NZ.

Enzyme and pathway databases

BioCyciYEAST:G3O-32421-MONOMER.

Miscellaneous databases

NextBioi967684.
PROiQ06137.

Family and domain databases

Gene3Di3.40.50.1240. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR003094. 6Pfruct_kin.
IPR013079. 6Phosfructo_kin.
IPR013078. His_Pase_superF_clade-1.
IPR029033. His_PPase_superfam.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR10606. PTHR10606. 2 hits.
PfamiPF01591. 6PF2K. 1 hit.
PF00300. His_Phos_1. 1 hit.
[Graphical view]
PRINTSiPR00991. 6PFRUCTKNASE.
SMARTiSM00855. PGAM. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF53254. SSF53254. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
    Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
    , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
    Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  5. "Identification of new genes regulated by the Crt1 transcription factor, an effector of the DNA damage checkpoint pathway in Saccharomyces cerevisiae."
    Zaim J., Speina E., Kierzek A.M.
    J. Biol. Chem. 280:28-37(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  6. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-6, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiYL345_YEAST
AccessioniPrimary (citable) accession number: Q06137
Secondary accession number(s): D6VYY4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3380 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.