Reviewed, UniProtKB/Swiss-Prot Q06137 (YL345_YEAST)

Names and origin

Protein names

Recommended name:
    Putative 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase YLR345W
Including the following 2 domains:
    1- Recommended name:
            6-phosphofructo-2-kinase
              EC=2.7.1.105
    2- Recommended name:
            Fructose-2,6-bisphosphatase
              EC=3.1.3.46

Gene names

Ordered Locus Names:

YLR345W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	509 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Synthesis and degradation of fructose 2,6-bisphosphate By similarity.
Catalytic activity	Beta-D-fructose 2,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate. ATP + D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Ref.2.
Induction	Expression is under the control of the RFX1 transcription factor. Ref.4
Miscellaneous	Present with 3380 molecules/cell in log phase SD medium. Ref.3
Sequence similarities	In the C-terminal section; belongs to the phosphoglycerate mutase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	fructose 2,6-bisphosphate metabolic process Ref.4 Non-traceable author statement. Source: SGD regulation of glycolysis Ref.4 Non-traceable author statement. Source: SGD
Cellular component	cytoplasm Ref.2 Inferred from direct assay. Source: SGD
Molecular function	6-phosphofructo-2-kinase activity Ref.4 Non-traceable author statement. Source: SGD ATP binding Inferred from electronic annotation. Source: UniProtKB-KW fructose-2,6-bisphosphate 2-phosphatase activity Inferred from electronic annotation. Source: EC protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	Q06146	1	EBI-33827,EBI-36149
MTR3	P48240	3	EBI-33827,EBI-1749
PRP45	P28004	2	EBI-33827,EBI-20640
RNR2	P09938	1	EBI-33827,EBI-15240
SFH1	Q06168	3	EBI-33827,EBI-35813
SIC1	P38634	1	EBI-33827,EBI-17127
TPS3	P38426	1	EBI-33827,EBI-19448

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 509	509	Putative 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase YLR345W		PRO_0000268185
Regions
	Nucleotide binding	90 – 97	8	ATP By similarity
	Region	6 – 291	286	6-phosphofructo-2-kinase By similarity
	Region	292 – 466	175	Fructose-2,6-bisphosphatase By similarity
Sites
	Active site	173	1	Potential
	Active site	459	1	Proton donor By similarity
	Binding site	237	1	Fructose-6-phosphate By similarity
Amino acid modifications
	Modified residue	6	1	Phosphoserine Ref.6
	Modified residue	75	1	Phosphoserine Ref.6
	Modified residue	104	1	Phosphothreonine Ref.5
	Modified residue	432	1	Phosphoserine Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Q06137-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 29718CA8ECA8EB4E	FASTA	509	58,386
	10 20 30 40 50 60 MPNVLSDDEE LLNGLGSEIM KPSRQGNHMA RTVKRWVNKE RATTADLKNV NIDGVHGPVN 70 80 90 100 110 120 TESYISPGQL YSTDSGNLFH AGRILVVLVG LPATSKTLLS VAITRYTRWL GVRTKSFHFS 130 140 150 160 170 180 EYKESAKNIP SDYFCVVPTS KEGVAFVEKL RMQMLNDILA FFNDLSGQLA IYDALNIRKI 190 200 210 220 230 240 DRKNLETTFS EIGVKVLFIE SIVSDQEIMN RNIALALESN DYKGLSTDEA IDEYMRRLSV 250 260 270 280 290 300 NEPYYEMMTH DEELSYIKYI NLGKQIIVKD NIHGYLVNKI VFFLMNLRQK KGCVYFARCG 310 320 330 340 350 360 TSDKDNYIHD EELNEEGIHY SQVLKDFVLQ RIKQKRQAKK NSDSLVEVID GSHDEDLKTS 370 380 390 400 410 420 LIVWTGPRKR THDTALFFSK EGIKVQQRSE LRQLNPGSIA DLTDQQIMDK FPSEYKESLK 430 440 450 460 470 480 DPYHFRFPRA ESYHDLAVRM EPLLLEMEHT SKDILIIAHE STLRVLYGYL MACTCVELPN 490 500 LNFTRDKLVE ISFSPFCNTV ELLNIPLTS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D. Nature 387:87-90(1997) [PubMed: 9169871] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[4]	"Identification of new genes regulated by the Crt1 transcription factor, an effector of the DNA damage checkpoint pathway in Saccharomyces cerevisiae." Zaim J., Speina E., Kierzek A.M. J. Biol. Chem. 280:28-37(2005) [PubMed: 15494396] [Abstract] Cited for: INDUCTION.
[5]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-104, MASS SPECTROMETRY.
[6]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-6; SER-75 AND SER-432, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U19028 Genomic DNA. Translation: AAB67253.1.
PIR	S51348.
RefSeq	NP_013449.1.
3D structure databases
HSSP	HSSP built from PDB template 2AXN based on UniProtKB Q16875.
SMR	Q06137. Positions 83-506.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1500N.
IntAct	Q06137. 21 interactions.
STRING	Q06137.
Proteomic databases
PeptideAtlas	Q06137.
Genome annotation databases
Ensembl	YLR345W; YLR345W; YLR345W; Saccharomyces cerevisiae. [Genome view]
GeneID	851059.
KEGG	sce:YLR345W.
NMPDR	fig|4932.3.peg.4470.
Organism-specific databases
CYGD	YLR345w.
SGD	S000004337. YLR345W.
Phylogenomic databases
eggNOG	fuNOG04023.
HOGENOM	HBG395770.
OMA	SYHDLAV.
OrthoDB	EOG9TQNS1.
PhylomeDB	Q06137.
Enzyme and pathway databases
BRENDA	2.7.1.105. 250. 3.1.3.46. 250.
Gene expression databases
ArrayExpress	Q06137.
Genevestigator	Q06137.
GermOnline	YLR345W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR003094. 6Pfruct_kin. IPR013079. 6Phosfructo_kin. IPR016260. Bifunct_6PFK/fruc_bisP_Ptase. IPR013078. PG_mutase. [Graphical view]
PANTHER	PTHR10606. 6Pfruct_kin. 1 hit.
Pfam	PF01591. 6PF2K. 1 hit. PF00300. PGAM. 1 hit. [Graphical view]
PIRSF	PIRSF000709. 6PFK_2-Ptase. 1 hit.
PRINTS	PR00991. 6PFRUCTKNASE.
SMART	SM00855. PGAM. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	967684.

Entry information

Entry name

YL345_YEAST

Accession

Primary (citable) accession number: Q06137

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	November 1, 1996
Last modified:	January 19, 2010
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)