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Protein

Anthranilate synthase component 2

Gene

trpG

Organism
Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Part of a heterotetrameric complex that catalyzes the two-step biosynthesis of anthranilate, an intermediate in the biosynthesis of L-tryptophan. In the first step, the glutamine-binding beta subunit (TrpG) of anthranilate synthase (AS) provides the glutamine amidotransferase activity which generates ammonia as a substrate that, along with chorismate, is used in the second step, catalyzed by the large alpha subunit of AS (TrpE) to produce anthranilate. In the absence of TrpG, TrpE can synthesize anthranilate directly from chorismate and high concentrations of ammonia (Probable).1 Publication

Catalytic activityi

Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.

Pathwayi: L-tryptophan biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-tryptophan from chorismate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Anthranilate synthase component 1 (trpE), Anthranilate synthase component 2 (trpG)
  2. Anthranilate phosphoribosyltransferase (trpD)
  3. N-(5'-phosphoribosyl)anthranilate isomerase (trpF)
  4. Indole-3-glycerol phosphate synthase (trpC)
  5. Tryptophan synthase beta chain 2 (trpB2), Tryptophan synthase beta chain 1 (trpB1), Tryptophan synthase alpha chain (trpA)
This subpathway is part of the pathway L-tryptophan biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-tryptophan from chorismate, the pathway L-tryptophan biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei84Nucleophile; for GATase activityPROSITE-ProRule annotation1
Binding sitei88GlutamineBy similarity1
Active sitei175For GATase activityPROSITE-ProRule annotation1
Active sitei177For GATase activityPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-3604.
UniPathwayiUPA00035; UER00040.

Protein family/group databases

MEROPSiC26.955.

Names & Taxonomyi

Protein namesi
Recommended name:
Anthranilate synthase component 2 (EC:4.1.3.27)
Short name:
AS
Short name:
ASII
Alternative name(s):
Anthranilate synthase, GATase component
Anthranilate synthase, glutamine amidotransferase component
Gene namesi
Name:trpG
Ordered Locus Names:SSO0894
OrganismiSulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Taxonomic identifieri273057 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
Proteomesi
  • UP000001974 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000569001 – 195Anthranilate synthase component 2Add BLAST195

Interactioni

Subunit structurei

Heterotetramer consisting of two non-identical subunits: a beta subunit (TrpG) and a large alpha subunit (TrpE).1 Publication

Protein-protein interaction databases

DIPiDIP-6203N.
IntActiQ06129. 1 interactor.
MINTiMINT-102261.
STRINGi273057.SSO0894.

Structurei

Secondary structure

1195
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 8Combined sources6
Helixi14 – 23Combined sources10
Beta strandi27 – 32Combined sources6
Turni33 – 35Combined sources3
Helixi38 – 44Combined sources7
Beta strandi47 – 51Combined sources5
Helixi61 – 64Combined sources4
Helixi67 – 74Combined sources8
Turni75 – 77Combined sources3
Beta strandi80 – 83Combined sources4
Helixi85 – 93Combined sources9
Beta strandi97 – 114Combined sources18
Turni122 – 125Combined sources4
Beta strandi128 – 141Combined sources14
Beta strandi147 – 156Combined sources10
Beta strandi159 – 175Combined sources17
Helixi184 – 194Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1QDLX-ray2.50B1-195[»]
ProteinModelPortaliQ06129.
SMRiQ06129.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ06129.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 195Glutamine amidotransferase type-1PROSITE-ProRule annotationAdd BLAST193

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni54 – 56Glutamine bindingBy similarity3
Regioni137 – 138Glutamine bindingBy similarity2

Sequence similaritiesi

Contains 1 glutamine amidotransferase type-1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiarCOG00086. Archaea.
COG0512. LUCA.
HOGENOMiHOG000025029.
InParanoidiQ06129.
KOiK01658.
OMAiPCSPDEA.

Family and domain databases

Gene3Di3.40.50.880. 1 hit.
InterProiIPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR006221. TrpG/PapA_dom.
[Graphical view]
PfamiPF00117. GATase. 1 hit.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR00566. trpG_papA. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q06129-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDLTLIIDNY DSFVYNIAQI VGELGSYPIV IRNDEISIKG IERIDPDRII
60 70 80 90 100
ISPGPGTPEK REDIGVSLDV IKYLGKRTPI LGVCLGHQAI GYAFGAKIRR
110 120 130 140 150
ARKVFHGKIS NIILVNNSPL SLYYGIAKEF KATRYHSLVV DEVHRPLIVD
160 170 180 190
AISAEDNEIM AIHHEEYPIY GVQFHPESVG TSLGYKILYN FLNRV
Length:195
Mass (Da):21,909
Last modified:June 1, 2001 - v2
Checksum:i155B62933E9CD33E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti49I → L in AAA73380 (PubMed:8416906).Curated1
Sequence conflicti49I → L in CAA90312 (PubMed:8416906).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M98048 Genomic DNA. Translation: AAA73380.1.
Z50014 Genomic DNA. Translation: CAA90312.1.
AE006641 Genomic DNA. Translation: AAK41176.1.
PIRiA99240.
B40635.
RefSeqiWP_009992310.1. NC_002754.1.

Genome annotation databases

EnsemblBacteriaiAAK41176; AAK41176; SSO0894.
GeneIDi27427214.
KEGGisso:SSO0894.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M98048 Genomic DNA. Translation: AAA73380.1.
Z50014 Genomic DNA. Translation: CAA90312.1.
AE006641 Genomic DNA. Translation: AAK41176.1.
PIRiA99240.
B40635.
RefSeqiWP_009992310.1. NC_002754.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1QDLX-ray2.50B1-195[»]
ProteinModelPortaliQ06129.
SMRiQ06129.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-6203N.
IntActiQ06129. 1 interactor.
MINTiMINT-102261.
STRINGi273057.SSO0894.

Protein family/group databases

MEROPSiC26.955.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK41176; AAK41176; SSO0894.
GeneIDi27427214.
KEGGisso:SSO0894.

Phylogenomic databases

eggNOGiarCOG00086. Archaea.
COG0512. LUCA.
HOGENOMiHOG000025029.
InParanoidiQ06129.
KOiK01658.
OMAiPCSPDEA.

Enzyme and pathway databases

UniPathwayiUPA00035; UER00040.
BioCyciMetaCyc:MONOMER-3604.

Miscellaneous databases

EvolutionaryTraceiQ06129.

Family and domain databases

Gene3Di3.40.50.880. 1 hit.
InterProiIPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR006221. TrpG/PapA_dom.
[Graphical view]
PfamiPF00117. GATase. 1 hit.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR00566. trpG_papA. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRPG_SULSO
AccessioniPrimary (citable) accession number: Q06129
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: June 1, 2001
Last modified: November 2, 2016
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.