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Protein

Anthranilate synthase component 2

Gene

trpG

Organism
Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Part of a heterotetrameric complex that catalyzes the two-step biosynthesis of anthranilate, an intermediate in the biosynthesis of L-tryptophan. In the first step, the glutamine-binding beta subunit (TrpG) of anthranilate synthase (AS) provides the glutamine amidotransferase activity which generates ammonia as a substrate that, along with chorismate, is used in the second step, catalyzed by the large alpha subunit of AS (TrpE) to produce anthranilate. In the absence of TrpG, TrpE can synthesize anthranilate directly from chorismate and high concentrations of ammonia (Probable).1 Publication

Catalytic activityi

Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei84 – 841For GATase activityPROSITE-ProRule annotation
Binding sitei84 – 841GlutamineBy similarity
Binding sitei88 – 881GlutamineBy similarity
Active sitei175 – 1751For GATase activityPROSITE-ProRule annotation
Active sitei177 – 1771For GATase activityPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-3604.
SSOL273057:GCH2-850-MONOMER.
UniPathwayiUPA00035; UER00040.

Protein family/group databases

MEROPSiC26.955.

Names & Taxonomyi

Protein namesi
Recommended name:
Anthranilate synthase component 2 (EC:4.1.3.27)
Short name:
AS
Short name:
ASII
Alternative name(s):
Anthranilate synthase, GATase component
Anthranilate synthase, glutamine amidotransferase component
Gene namesi
Name:trpG
Ordered Locus Names:SSO0894
OrganismiSulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Taxonomic identifieri273057 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
ProteomesiUP000001974 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 195195Anthranilate synthase component 2PRO_0000056900Add
BLAST

Interactioni

Subunit structurei

Heterotetramer consisting of two non-identical subunits: a beta subunit (TrpG) and a large alpha subunit (TrpE).1 Publication

Protein-protein interaction databases

DIPiDIP-6203N.
IntActiQ06129. 1 interaction.
MINTiMINT-102261.
STRINGi273057.SSO0894.

Structurei

Secondary structure

1
195
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 86Combined sources
Helixi14 – 2310Combined sources
Beta strandi27 – 326Combined sources
Turni33 – 353Combined sources
Helixi38 – 447Combined sources
Beta strandi47 – 515Combined sources
Helixi61 – 644Combined sources
Helixi67 – 748Combined sources
Turni75 – 773Combined sources
Beta strandi80 – 834Combined sources
Helixi85 – 939Combined sources
Beta strandi97 – 11418Combined sources
Turni122 – 1254Combined sources
Beta strandi128 – 14114Combined sources
Beta strandi147 – 15610Combined sources
Beta strandi159 – 17517Combined sources
Helixi184 – 19411Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1QDLX-ray2.50B1-195[»]
ProteinModelPortaliQ06129.
SMRiQ06129. Positions 1-195.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ06129.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 195193Glutamine amidotransferase type-1PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni54 – 563Glutamine bindingBy similarity
Regioni137 – 1382Glutamine bindingBy similarity

Sequence similaritiesi

Contains 1 glutamine amidotransferase type-1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiCOG0512.
HOGENOMiHOG000025029.
InParanoidiQ06129.
KOiK01658.
OMAiTEHGHAM.

Family and domain databases

Gene3Di3.40.50.880. 1 hit.
InterProiIPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR006221. TrpG/PapA_dom.
[Graphical view]
PfamiPF00117. GATase. 1 hit.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR00566. trpG_papA. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q06129-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDLTLIIDNY DSFVYNIAQI VGELGSYPIV IRNDEISIKG IERIDPDRII
60 70 80 90 100
ISPGPGTPEK REDIGVSLDV IKYLGKRTPI LGVCLGHQAI GYAFGAKIRR
110 120 130 140 150
ARKVFHGKIS NIILVNNSPL SLYYGIAKEF KATRYHSLVV DEVHRPLIVD
160 170 180 190
AISAEDNEIM AIHHEEYPIY GVQFHPESVG TSLGYKILYN FLNRV
Length:195
Mass (Da):21,909
Last modified:June 1, 2001 - v2
Checksum:i155B62933E9CD33E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti49 – 491I → L in AAA73380 (PubMed:8416906).Curated
Sequence conflicti49 – 491I → L in CAA90312 (PubMed:8416906).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M98048 Genomic DNA. Translation: AAA73380.1.
Z50014 Genomic DNA. Translation: CAA90312.1.
AE006641 Genomic DNA. Translation: AAK41176.1.
PIRiA99240.
B40635.
RefSeqiNP_342386.1. NC_002754.1.
WP_009992310.1. NC_002754.1.

Genome annotation databases

EnsemblBacteriaiAAK41176; AAK41176; SSO0894.
GeneIDi1455143.
KEGGisso:SSO0894.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M98048 Genomic DNA. Translation: AAA73380.1.
Z50014 Genomic DNA. Translation: CAA90312.1.
AE006641 Genomic DNA. Translation: AAK41176.1.
PIRiA99240.
B40635.
RefSeqiNP_342386.1. NC_002754.1.
WP_009992310.1. NC_002754.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1QDLX-ray2.50B1-195[»]
ProteinModelPortaliQ06129.
SMRiQ06129. Positions 1-195.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-6203N.
IntActiQ06129. 1 interaction.
MINTiMINT-102261.
STRINGi273057.SSO0894.

Protein family/group databases

MEROPSiC26.955.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK41176; AAK41176; SSO0894.
GeneIDi1455143.
KEGGisso:SSO0894.

Phylogenomic databases

eggNOGiCOG0512.
HOGENOMiHOG000025029.
InParanoidiQ06129.
KOiK01658.
OMAiTEHGHAM.

Enzyme and pathway databases

UniPathwayiUPA00035; UER00040.
BioCyciMetaCyc:MONOMER-3604.
SSOL273057:GCH2-850-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ06129.

Family and domain databases

Gene3Di3.40.50.880. 1 hit.
InterProiIPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR006221. TrpG/PapA_dom.
[Graphical view]
PfamiPF00117. GATase. 1 hit.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR00566. trpG_papA. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Tryptophan biosynthesis genes trpEGC in the thermoacidophilic archaebacterium Sulfolobus solfataricus."
    Tutino M.L., Scarano G., Marino G., Sannia G., Cubellis M.V.
    J. Bacteriol. 175:299-302(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: DSM 5833 / MT-4.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
  3. "The crystal structure of anthranilate synthase from Sulfolobus solfataricus: functional implications."
    Knoechel T., Ivens A., Hester G., Gonzalez A., Bauerle R., Wilmanns M., Kirschner K., Jansonius J.N.
    Proc. Natl. Acad. Sci. U.S.A. 96:9479-9484(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS), FUNCTION, SUBUNIT.

Entry informationi

Entry nameiTRPG_SULSO
AccessioniPrimary (citable) accession number: Q06129
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: June 1, 2001
Last modified: May 27, 2015
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.