Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q06129

- TRPG_SULSO

UniProt

Q06129 - TRPG_SULSO

Protein

Anthranilate synthase component 2

Gene

trpG

Organism
Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 2 (01 Jun 2001)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Part of a heterotetrameric complex that catalyzes the two-step biosynthesis of anthranilate, an intermediate in the biosynthesis of L-tryptophan. In the first step, the glutamine-binding beta subunit (TrpG) of anthranilate synthase (AS) provides the glutamine amidotransferase activity which generates ammonia as a substrate that, along with chorismate, is used in the second step, catalyzed by the large alpha subunit of AS (TrpE) to produce anthranilate. In the absence of TrpG, TrpE can synthesize anthranilate directly from chorismate and high concentrations of ammonia Probable.1 Publication

    Catalytic activityi

    Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei84 – 841For GATase activityPROSITE-ProRule annotation
    Binding sitei84 – 841GlutamineBy similarity
    Binding sitei88 – 881GlutamineBy similarity
    Active sitei175 – 1751For GATase activityPROSITE-ProRule annotation
    Active sitei177 – 1771For GATase activityPROSITE-ProRule annotation

    GO - Molecular functioni

    1. anthranilate synthase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glutamine metabolic process Source: UniProtKB-KW
    2. tryptophan biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-3604.
    SSOL273057:GCH2-850-MONOMER.
    UniPathwayiUPA00035; UER00040.

    Protein family/group databases

    MEROPSiC26.955.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Anthranilate synthase component 2 (EC:4.1.3.27)
    Short name:
    AS
    Short name:
    ASII
    Alternative name(s):
    Anthranilate synthase, GATase component
    Anthranilate synthase, glutamine amidotransferase component
    Gene namesi
    Name:trpG
    Ordered Locus Names:SSO0894
    OrganismiSulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
    Taxonomic identifieri273057 [NCBI]
    Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
    ProteomesiUP000001974: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 195195Anthranilate synthase component 2PRO_0000056900Add
    BLAST

    Interactioni

    Subunit structurei

    Heterotetramer consisting of two non-identical subunits: a beta subunit (TrpG) and a large alpha subunit (TrpE).1 Publication

    Protein-protein interaction databases

    DIPiDIP-6203N.
    IntActiQ06129. 1 interaction.
    MINTiMINT-102261.
    STRINGi273057.SSO0894.

    Structurei

    Secondary structure

    1
    195
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi3 – 86
    Helixi14 – 2310
    Beta strandi27 – 326
    Turni33 – 353
    Helixi38 – 447
    Beta strandi47 – 515
    Helixi61 – 644
    Helixi67 – 748
    Turni75 – 773
    Beta strandi80 – 834
    Helixi85 – 939
    Beta strandi97 – 11418
    Turni122 – 1254
    Beta strandi128 – 14114
    Beta strandi147 – 15610
    Beta strandi159 – 17517
    Helixi184 – 19411

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1QDLX-ray2.50B1-195[»]
    ProteinModelPortaliQ06129.
    SMRiQ06129. Positions 1-195.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ06129.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini3 – 195193Glutamine amidotransferase type-1PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni54 – 563Glutamine bindingBy similarity
    Regioni137 – 1382Glutamine bindingBy similarity

    Sequence similaritiesi

    Contains 1 glutamine amidotransferase type-1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Glutamine amidotransferase

    Phylogenomic databases

    eggNOGiCOG0512.
    HOGENOMiHOG000025029.
    KOiK01658.
    OMAiDAYSKED.

    Family and domain databases

    Gene3Di3.40.50.880. 1 hit.
    InterProiIPR029062. Class_I_gatase-like.
    IPR017926. GATASE.
    IPR006221. TrpG/PapA_dom.
    [Graphical view]
    PfamiPF00117. GATase. 1 hit.
    [Graphical view]
    SUPFAMiSSF52317. SSF52317. 1 hit.
    TIGRFAMsiTIGR00566. trpG_papA. 1 hit.
    PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q06129-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDLTLIIDNY DSFVYNIAQI VGELGSYPIV IRNDEISIKG IERIDPDRII    50
    ISPGPGTPEK REDIGVSLDV IKYLGKRTPI LGVCLGHQAI GYAFGAKIRR 100
    ARKVFHGKIS NIILVNNSPL SLYYGIAKEF KATRYHSLVV DEVHRPLIVD 150
    AISAEDNEIM AIHHEEYPIY GVQFHPESVG TSLGYKILYN FLNRV 195
    Length:195
    Mass (Da):21,909
    Last modified:June 1, 2001 - v2
    Checksum:i155B62933E9CD33E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti49 – 491I → L in AAA73380. (PubMed:8416906)Curated
    Sequence conflicti49 – 491I → L in CAA90312. (PubMed:8416906)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M98048 Genomic DNA. Translation: AAA73380.1.
    Z50014 Genomic DNA. Translation: CAA90312.1.
    AE006641 Genomic DNA. Translation: AAK41176.1.
    PIRiA99240.
    B40635.
    RefSeqiNP_342386.1. NC_002754.1.
    WP_009992310.1. NC_002754.1.

    Genome annotation databases

    EnsemblBacteriaiAAK41176; AAK41176; SSO0894.
    GeneIDi1455143.
    KEGGisso:SSO0894.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M98048 Genomic DNA. Translation: AAA73380.1 .
    Z50014 Genomic DNA. Translation: CAA90312.1 .
    AE006641 Genomic DNA. Translation: AAK41176.1 .
    PIRi A99240.
    B40635.
    RefSeqi NP_342386.1. NC_002754.1.
    WP_009992310.1. NC_002754.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1QDL X-ray 2.50 B 1-195 [» ]
    ProteinModelPortali Q06129.
    SMRi Q06129. Positions 1-195.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-6203N.
    IntActi Q06129. 1 interaction.
    MINTi MINT-102261.
    STRINGi 273057.SSO0894.

    Protein family/group databases

    MEROPSi C26.955.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAK41176 ; AAK41176 ; SSO0894 .
    GeneIDi 1455143.
    KEGGi sso:SSO0894.

    Phylogenomic databases

    eggNOGi COG0512.
    HOGENOMi HOG000025029.
    KOi K01658.
    OMAi DAYSKED.

    Enzyme and pathway databases

    UniPathwayi UPA00035 ; UER00040 .
    BioCyci MetaCyc:MONOMER-3604.
    SSOL273057:GCH2-850-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei Q06129.

    Family and domain databases

    Gene3Di 3.40.50.880. 1 hit.
    InterProi IPR029062. Class_I_gatase-like.
    IPR017926. GATASE.
    IPR006221. TrpG/PapA_dom.
    [Graphical view ]
    Pfami PF00117. GATase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52317. SSF52317. 1 hit.
    TIGRFAMsi TIGR00566. trpG_papA. 1 hit.
    PROSITEi PS51273. GATASE_TYPE_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Tryptophan biosynthesis genes trpEGC in the thermoacidophilic archaebacterium Sulfolobus solfataricus."
      Tutino M.L., Scarano G., Marino G., Sannia G., Cubellis M.V.
      J. Bacteriol. 175:299-302(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: DSM 5833 / MT-4.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
    3. "The crystal structure of anthranilate synthase from Sulfolobus solfataricus: functional implications."
      Knoechel T., Ivens A., Hester G., Gonzalez A., Bauerle R., Wilmanns M., Kirschner K., Jansonius J.N.
      Proc. Natl. Acad. Sci. U.S.A. 96:9479-9484(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS), FUNCTION, SUBUNIT.

    Entry informationi

    Entry nameiTRPG_SULSO
    AccessioniPrimary (citable) accession number: Q06129
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 112 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3