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Q06129

- TRPG_SULSO

UniProt

Q06129 - TRPG_SULSO

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Protein

Anthranilate synthase component 2

Gene
trpG, SSO0894
Organism
Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Part of a heterotetrameric complex that catalyzes the two-step biosynthesis of anthranilate, an intermediate in the biosynthesis of L-tryptophan. In the first step, the glutamine-binding beta subunit (TrpG) of anthranilate synthase (AS) provides the glutamine amidotransferase activity which generates ammonia as a substrate that, along with chorismate, is used in the second step, catalyzed by the large alpha subunit of AS (TrpE) to produce anthranilate. In the absence of TrpG, TrpE can synthesize anthranilate directly from chorismate and high concentrations of ammonia Inferred.1 Publication

Catalytic activityi

Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei84 – 841For GATase activity By similarity
Binding sitei84 – 841Glutamine By similarity
Binding sitei88 – 881Glutamine By similarity
Active sitei175 – 1751For GATase activity By similarity
Active sitei177 – 1771For GATase activity By similarity

GO - Molecular functioni

  1. anthranilate synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. glutamine metabolic process Source: UniProtKB-KW
  2. tryptophan biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-3604.
SSOL273057:GCH2-850-MONOMER.
UniPathwayiUPA00035; UER00040.

Protein family/group databases

MEROPSiC26.955.

Names & Taxonomyi

Protein namesi
Recommended name:
Anthranilate synthase component 2 (EC:4.1.3.27)
Short name:
AS
Short name:
ASII
Alternative name(s):
Anthranilate synthase, GATase component
Anthranilate synthase, glutamine amidotransferase component
Gene namesi
Name:trpG
Ordered Locus Names:SSO0894
OrganismiSulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Taxonomic identifieri273057 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
ProteomesiUP000001974: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 195195Anthranilate synthase component 2PRO_0000056900Add
BLAST

Interactioni

Subunit structurei

Heterotetramer consisting of two non-identical subunits: a beta subunit (TrpG) and a large alpha subunit (TrpE).1 Publication

Protein-protein interaction databases

DIPiDIP-6203N.
IntActiQ06129. 1 interaction.
MINTiMINT-102261.
STRINGi273057.SSO0894.

Structurei

Secondary structure

1
195
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 86
Helixi14 – 2310
Beta strandi27 – 326
Turni33 – 353
Helixi38 – 447
Beta strandi47 – 515
Helixi61 – 644
Helixi67 – 748
Turni75 – 773
Beta strandi80 – 834
Helixi85 – 939
Beta strandi97 – 11418
Turni122 – 1254
Beta strandi128 – 14114
Beta strandi147 – 15610
Beta strandi159 – 17517
Helixi184 – 19411

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1QDLX-ray2.50B1-195[»]
ProteinModelPortaliQ06129.
SMRiQ06129. Positions 1-195.

Miscellaneous databases

EvolutionaryTraceiQ06129.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 195193Glutamine amidotransferase type-1Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni54 – 563Glutamine binding By similarity
Regioni137 – 1382Glutamine binding By similarity

Sequence similaritiesi

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiCOG0512.
HOGENOMiHOG000025029.
KOiK01658.
OMAiDAYSKED.

Family and domain databases

Gene3Di3.40.50.880. 1 hit.
InterProiIPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR006221. TrpG/PapA_dom.
[Graphical view]
PfamiPF00117. GATase. 1 hit.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR00566. trpG_papA. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q06129-1 [UniParc]FASTAAdd to Basket

« Hide

MDLTLIIDNY DSFVYNIAQI VGELGSYPIV IRNDEISIKG IERIDPDRII    50
ISPGPGTPEK REDIGVSLDV IKYLGKRTPI LGVCLGHQAI GYAFGAKIRR 100
ARKVFHGKIS NIILVNNSPL SLYYGIAKEF KATRYHSLVV DEVHRPLIVD 150
AISAEDNEIM AIHHEEYPIY GVQFHPESVG TSLGYKILYN FLNRV 195
Length:195
Mass (Da):21,909
Last modified:June 1, 2001 - v2
Checksum:i155B62933E9CD33E
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti49 – 491I → L in AAA73380. 1 Publication
Sequence conflicti49 – 491I → L in CAA90312. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M98048 Genomic DNA. Translation: AAA73380.1.
Z50014 Genomic DNA. Translation: CAA90312.1.
AE006641 Genomic DNA. Translation: AAK41176.1.
PIRiA99240.
B40635.
RefSeqiNP_342386.1. NC_002754.1.
WP_009992310.1. NC_002754.1.

Genome annotation databases

EnsemblBacteriaiAAK41176; AAK41176; SSO0894.
GeneIDi1455143.
KEGGisso:SSO0894.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M98048 Genomic DNA. Translation: AAA73380.1 .
Z50014 Genomic DNA. Translation: CAA90312.1 .
AE006641 Genomic DNA. Translation: AAK41176.1 .
PIRi A99240.
B40635.
RefSeqi NP_342386.1. NC_002754.1.
WP_009992310.1. NC_002754.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1QDL X-ray 2.50 B 1-195 [» ]
ProteinModelPortali Q06129.
SMRi Q06129. Positions 1-195.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-6203N.
IntActi Q06129. 1 interaction.
MINTi MINT-102261.
STRINGi 273057.SSO0894.

Protein family/group databases

MEROPSi C26.955.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAK41176 ; AAK41176 ; SSO0894 .
GeneIDi 1455143.
KEGGi sso:SSO0894.

Phylogenomic databases

eggNOGi COG0512.
HOGENOMi HOG000025029.
KOi K01658.
OMAi DAYSKED.

Enzyme and pathway databases

UniPathwayi UPA00035 ; UER00040 .
BioCyci MetaCyc:MONOMER-3604.
SSOL273057:GCH2-850-MONOMER.

Miscellaneous databases

EvolutionaryTracei Q06129.

Family and domain databases

Gene3Di 3.40.50.880. 1 hit.
InterProi IPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR006221. TrpG/PapA_dom.
[Graphical view ]
Pfami PF00117. GATase. 1 hit.
[Graphical view ]
SUPFAMi SSF52317. SSF52317. 1 hit.
TIGRFAMsi TIGR00566. trpG_papA. 1 hit.
PROSITEi PS51273. GATASE_TYPE_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Tryptophan biosynthesis genes trpEGC in the thermoacidophilic archaebacterium Sulfolobus solfataricus."
    Tutino M.L., Scarano G., Marino G., Sannia G., Cubellis M.V.
    J. Bacteriol. 175:299-302(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: DSM 5833 / MT-4.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
  3. "The crystal structure of anthranilate synthase from Sulfolobus solfataricus: functional implications."
    Knoechel T., Ivens A., Hester G., Gonzalez A., Bauerle R., Wilmanns M., Kirschner K., Jansonius J.N.
    Proc. Natl. Acad. Sci. U.S.A. 96:9479-9484(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS), FUNCTION, SUBUNIT.

Entry informationi

Entry nameiTRPG_SULSO
AccessioniPrimary (citable) accession number: Q06129
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: June 1, 2001
Last modified: September 3, 2014
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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