Reviewed, UniProtKB/Swiss-Prot Q06129 (TRPG_SULSO)

Names and origin

Protein names

Recommended name:
    Anthranilate synthase component II
    EC=4.1.3.27
Alternative name(s):
    Glutamine amido-transferase

Gene names

Name:	trpG
Ordered Locus Names:	SSO0894

Organism

Sulfolobus solfataricus [Complete proteome] [HAMAP]

Taxonomic identifier

2287 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus

Protein attributes

Sequence length	195 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.
Pathway	Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 1/5.
Subunit structure	Tetramer of two components I and two components II.
Miscellaneous	Component I catalyzes the formation of anthranilate using ammonia rather than glutamine, whereas component II provides glutamine amidotransferase activity.
Sequence similarities	Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis Tryptophan biosynthesis
Domain	Glutamine amidotransferase
Molecular function	Lyase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW tryptophan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	anthranilate synthase activity Inferred from electronic annotation. Source: EC protein binding Ref.3 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
trpE	Q06128	1	EBI-1028067,EBI-1028059

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 195

195

Anthranilate synthase component II

PRO_0000056900

Regions

Domain

3 – 195

193

Glutamine amidotransferase type-1

Sites

Active site

84

1

By similarity

Active site

175

1

By similarity

Active site

177

1

By similarity

Experimental info

Sequence conflict

49

1

I → L in AAA73380. Ref.1

Sequence conflict

49

1

I → L in CAA90312. Ref.1

Secondary structure

1

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195

Helix Strand Turn

Details...

Beta strand

3 – 8

6

Helix

14 – 23

10

Beta strand

27 – 32

6

Turn

33 – 35

3

Helix

38 – 44

7

Beta strand

47 – 51

5

Helix

61 – 64

4

Helix

67 – 74

8

Turn

75 – 77

3

Beta strand

80 – 83

4

Helix

85 – 93

9

Beta strand

97 – 114

18

Turn

122 – 125

4

Beta strand

128 – 141

14

Beta strand

147 – 156

10

Beta strand

159 – 175

17

Helix

184 – 194

11

Sequences

	Sequence		Length	Mass (Da)
	Q06129-1 [UniParc]. Last modified June 1, 2001. Version 2. Checksum: 155B62933E9CD33E	FASTA	195	21,909
	10 20 30 40 50 60 MDLTLIIDNY DSFVYNIAQI VGELGSYPIV IRNDEISIKG IERIDPDRII ISPGPGTPEK 70 80 90 100 110 120 REDIGVSLDV IKYLGKRTPI LGVCLGHQAI GYAFGAKIRR ARKVFHGKIS NIILVNNSPL 130 140 150 160 170 180 SLYYGIAKEF KATRYHSLVV DEVHRPLIVD AISAEDNEIM AIHHEEYPIY GVQFHPESVG 190 TSLGYKILYN FLNRV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Tryptophan biosynthesis genes trpEGC in the thermoacidophilic archaebacterium Sulfolobus solfataricus." Tutino M.L., Scarano G., Marino G., Sannia G., Cubellis M.V. J. Bacteriol. 175:299-302(1993) [PubMed: 8416906] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DSM 5833 / MT-4.
[2]	"The complete genome of the crenarchaeon Sulfolobus solfataricus P2." She Q., Singh R.K., Confalonieri F., Zivanovic Y., Allard G., Awayez M.J., Chan-Weiher C.C.-Y., Clausen I.G., Curtis B.A., De Moors A., Erauso G., Fletcher C., Gordon P.M.K., Heikamp-de Jong I., Jeffries A.C., Kozera C.J., Medina N., Peng X. , Thi-Ngoc H.P., Redder P., Schenk M.E., Theriault C., Tolstrup N., Charlebois R.L., Doolittle W.F., Duguet M., Gaasterland T., Garrett R.A., Ragan M.A., Sensen C.W., Van der Oost J. Proc. Natl. Acad. Sci. U.S.A. 98:7835-7840(2001) [PubMed: 11427726] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
[3]	"The crystal structure of anthranilate synthase from Sulfolobus solfataricus: functional implications." Knoechel T., Ivens A., Hester G., Gonzalez A., Bauerle R., Wilmanns M., Kirschner K., Jansonius J.N. Proc. Natl. Acad. Sci. U.S.A. 96:9479-9484(1999) [PubMed: 10449718] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).

Cross-references

Sequence databases

EMBLGenBankDDBJ

M98048 Genomic DNA. Translation: AAA73380.1.
Z50014 Genomic DNA. Translation: CAA90312.1.
AE006641 Genomic DNA. Translation: AAK41176.1.

PIR

A99240.
B40635.

RefSeq

NP_342386.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1QDL	X-ray	2.50	B	1-195	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:6203N.

IntAct

Q06129. 1 interaction.

Genome annotation databases

GeneID

1455143.

GenomeReviews

Gene locus SSO0894 in contig AE006641_GR.

KEGG

sso:SSO0894.

NMPDR

fig|273057.1.peg.809.

Organism-specific databases

CMR

Search...

Phylogenomic databases

HOGENOM

Q06129.

OMA

Q06129. EDNEIMA.

Enzyme and pathway databases

BioCyc

MetaCyc:MON-3604.
SSOL273057:SSO0894-MON.

BRENDA

4.1.3.27. 2070.

Family and domain databases

InterPro

IPR006220. Anth_synthII.
IPR001317. CarbamoylP_synth_GATase.
IPR011702. GATASE.
IPR017926. GATASE_1.
IPR000991. GATase_class1_C.
IPR006221. TrpG_papA.
[Graphical view]

Pfam

PF00117. GATase. 1 hit.
[Graphical view]

PRINTS

PR00097. ANTSNTHASEII.
PR00099. CPSGATASE.
PR00096. GATASE.

TIGRFAMs

TIGR00566. trpG_papA. 1 hit.

PROSITE

PS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

TRPG_SULSO

Accession

Primary (citable) accession number: Q06129

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	June 1, 2001
Last modified:	June 16, 2009
This is version 79 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)