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Q06128

- TRPE_SULSO

UniProt

Q06128 - TRPE_SULSO

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Protein

Anthranilate synthase component 1

Gene

trpE

Organism
Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Part of a heterotetrameric complex that catalyzes the two-step biosynthesis of anthranilate, an intermediate in the biosynthesis of L-tryptophan. In the first step, the glutamine-binding beta subunit (TrpG) of anthranilate synthase (AS) provides the glutamine amidotransferase activity which generates ammonia as a substrate that, along with chorismate, is used in the second step, catalyzed by the large alpha subunit of AS (TrpE) to produce anthranilate. In the absence of TrpG, TrpE can synthesize anthranilate directly from chorismate and high concentrations of ammonia.1 Publication

Catalytic activityi

Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.

Cofactori

Mg2+By similarityNote: Binds 1 Mg(2+) ion per subunit.By similarity

Enzyme regulationi

Cooperatively feedback inhibited by tryptophan.1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei31 – 311TryptophanBy similarity
Metal bindingi269 – 2691MagnesiumBy similarity
Binding sitei357 – 3571SubstrateBy similarity
Binding sitei377 – 3771SubstrateBy similarity
Binding sitei393 – 3931Substrate; via amide nitrogenBy similarity
Metal bindingi406 – 4061MagnesiumBy similarity

GO - Molecular functioni

  1. anthranilate synthase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. tryptophan biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-3603.
SSOL273057:GCH2-849-MONOMER.
UniPathwayiUPA00035; UER00040.

Names & Taxonomyi

Protein namesi
Recommended name:
Anthranilate synthase component 1 (EC:4.1.3.27)
Short name:
AS
Short name:
ASI
Gene namesi
Name:trpE
Ordered Locus Names:SSO0893
OrganismiSulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Taxonomic identifieri273057 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
ProteomesiUP000001974: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 421421Anthranilate synthase component 1PRO_0000154132Add
BLAST

Interactioni

Subunit structurei

Heterotetramer consisting of two non-identical subunits: a beta subunit (TrpG) and a large alpha subunit (TrpE).1 Publication

Protein-protein interaction databases

DIPiDIP-6202N.
IntActiQ06128. 1 interaction.
MINTiMINT-102245.
STRINGi273057.SSO0893.

Structurei

Secondary structure

421
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi1 – 55Combined sources
Helixi6 – 83Combined sources
Helixi12 – 2211Combined sources
Beta strandi24 – 307Combined sources
Beta strandi41 – 466Combined sources
Beta strandi52 – 543Combined sources
Helixi58 – 636Combined sources
Turni64 – 685Combined sources
Beta strandi75 – 773Combined sources
Beta strandi79 – 868Combined sources
Helixi88 – 936Combined sources
Beta strandi103 – 1053Combined sources
Beta strandi109 – 1135Combined sources
Beta strandi116 – 1227Combined sources
Helixi123 – 1253Combined sources
Beta strandi127 – 1326Combined sources
Beta strandi146 – 15510Combined sources
Helixi157 – 17317Combined sources
Beta strandi177 – 19115Combined sources
Helixi194 – 20310Combined sources
Beta strandi207 – 2148Combined sources
Beta strandi217 – 2248Combined sources
Beta strandi226 – 2316Combined sources
Beta strandi234 – 2374Combined sources
Beta strandi240 – 2456Combined sources
Helixi250 – 26112Combined sources
Helixi264 – 28219Combined sources
Beta strandi291 – 30010Combined sources
Beta strandi302 – 31514Combined sources
Helixi321 – 3288Combined sources
Helixi332 – 3343Combined sources
Beta strandi335 – 3384Combined sources
Helixi339 – 34911Combined sources
Beta strandi350 – 3534Combined sources
Turni355 – 3584Combined sources
Beta strandi359 – 3657Combined sources
Beta strandi370 – 3756Combined sources
Beta strandi377 – 3826Combined sources
Beta strandi385 – 39410Combined sources
Helixi400 – 41718Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1QDLX-ray2.50A1-421[»]
ProteinModelPortaliQ06128.
SMRiQ06128. Positions 1-421.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ06128.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni207 – 2093Tryptophan bindingBy similarity
Regioni242 – 2432Substrate bindingBy similarity
Regioni391 – 3933Substrate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0147.
HOGENOMiHOG000025142.
InParanoidiQ06128.
KOiK01657.
OMAiADFAITI.

Family and domain databases

Gene3Di3.60.120.10. 1 hit.
InterProiIPR005801. ADC_synthase.
IPR019999. Anth_synth_I_like.
IPR006805. Anth_synth_I_N.
IPR010116. Anthranilate_synth_I_arc_typ.
IPR015890. Chorismate-bd_C.
[Graphical view]
PfamiPF04715. Anth_synt_I_N. 1 hit.
PF00425. Chorismate_bind. 1 hit.
[Graphical view]
PRINTSiPR00095. ANTSNTHASEI.
SUPFAMiSSF56322. SSF56322. 1 hit.
TIGRFAMsiTIGR01820. TrpE-arch. 1 hit.

Sequencei

Sequence statusi: Complete.

Q06128-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEVHPISEFA SPFEVFKCIE RDFKVAGLLE SIGGPQYKAR YSVIAWSTNG
60 70 80 90 100
YLKIHDDPVN ILNGYLKDLK LADIPGLFKG GMIGYISYDA VRFWEKIRDL
110 120 130 140 150
KPAAEDWPYA EFFTPDNIII YDHNEGKVYV NADLSSVGGC GDIGEFKVSF
160 170 180 190 200
YDESLNKNSY ERIVSESLEY IRSGYIFQVV LSRFYRYIFS GDPLRIYYNL
210 220 230 240 250
RRINPSPYMF YLKFDEKYLI GSSPELLFRV QDNIVETYPI AGTRPRGADQ
260 270 280 290 300
EEDLKLELEL MNSEKDKAEH LMLVDLARND LGKVCVPGTV KVPELMYVEK
310 320 330 340 350
YSHVQHIVSK VIGTLKKKYN ALNVLSATFP AGTVSGAPKP MAMNIIETLE
360 370 380 390 400
EYKRGPYAGA VGFISADGNA EFAIAIRTAF LNKELLRIHA GAGIVYDSNP
410 420
ESEYFETEHK LKALKTAIGV R
Length:421
Mass (Da):47,652
Last modified:June 20, 2001 - v2
Checksum:iBD49BD7771ECC6EE
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti337 – 3371A → R in AAA73379. (PubMed:8416906)Curated
Sequence conflicti337 – 3371A → R in CAA90311. (PubMed:9016772)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M98048 Genomic DNA. Translation: AAA73379.1.
Z50014 Genomic DNA. Translation: CAA90311.1.
AE006641 Genomic DNA. Translation: AAK41175.1.
PIRiH90239.
JC5323.
RefSeqiNP_342385.1. NC_002754.1.

Genome annotation databases

EnsemblBacteriaiAAK41175; AAK41175; SSO0893.
GeneIDi1455142.
KEGGisso:SSO0893.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M98048 Genomic DNA. Translation: AAA73379.1 .
Z50014 Genomic DNA. Translation: CAA90311.1 .
AE006641 Genomic DNA. Translation: AAK41175.1 .
PIRi H90239.
JC5323.
RefSeqi NP_342385.1. NC_002754.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1QDL X-ray 2.50 A 1-421 [» ]
ProteinModelPortali Q06128.
SMRi Q06128. Positions 1-421.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-6202N.
IntActi Q06128. 1 interaction.
MINTi MINT-102245.
STRINGi 273057.SSO0893.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAK41175 ; AAK41175 ; SSO0893 .
GeneIDi 1455142.
KEGGi sso:SSO0893.

Phylogenomic databases

eggNOGi COG0147.
HOGENOMi HOG000025142.
InParanoidi Q06128.
KOi K01657.
OMAi ADFAITI.

Enzyme and pathway databases

UniPathwayi UPA00035 ; UER00040 .
BioCyci MetaCyc:MONOMER-3603.
SSOL273057:GCH2-849-MONOMER.

Miscellaneous databases

EvolutionaryTracei Q06128.

Family and domain databases

Gene3Di 3.60.120.10. 1 hit.
InterProi IPR005801. ADC_synthase.
IPR019999. Anth_synth_I_like.
IPR006805. Anth_synth_I_N.
IPR010116. Anthranilate_synth_I_arc_typ.
IPR015890. Chorismate-bd_C.
[Graphical view ]
Pfami PF04715. Anth_synt_I_N. 1 hit.
PF00425. Chorismate_bind. 1 hit.
[Graphical view ]
PRINTSi PR00095. ANTSNTHASEI.
SUPFAMi SSF56322. SSF56322. 1 hit.
TIGRFAMsi TIGR01820. TrpE-arch. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Tryptophan biosynthesis genes trpEGC in the thermoacidophilic archaebacterium Sulfolobus solfataricus."
    Tutino M.L., Scarano G., Marino G., Sannia G., Cubellis M.V.
    J. Bacteriol. 175:299-302(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: DSM 5833 / MT-4.
  2. "Expression of Sulfolobus solfataricus trpE and trpG genes in E. coli."
    Tutino M.L., Tosco A., Marino G., Sannia G.
    Biochem. Biophys. Res. Commun. 230:306-310(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: DSM 5833 / MT-4.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
  4. "The crystal structure of anthranilate synthase from Sulfolobus solfataricus: functional implications."
    Knoechel T., Ivens A., Hester G., Gonzalez A., Bauerle R., Wilmanns M., Kirschner K., Jansonius J.N.
    Proc. Natl. Acad. Sci. U.S.A. 96:9479-9484(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS), FUNCTION, ENZYME REGULATION, SUBUNIT.

Entry informationi

Entry nameiTRPE_SULSO
AccessioniPrimary (citable) accession number: Q06128
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: June 20, 2001
Last modified: November 26, 2014
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3