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Q06128

- TRPE_SULSO

UniProt

Q06128 - TRPE_SULSO

Protein

Anthranilate synthase component 1

Gene

trpE

Organism
Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 118 (01 Oct 2014)
      Sequence version 2 (20 Jun 2001)
      Previous versions | rss
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    Functioni

    Part of a heterotetrameric complex that catalyzes the two-step biosynthesis of anthranilate, an intermediate in the biosynthesis of L-tryptophan. In the first step, the glutamine-binding beta subunit (TrpG) of anthranilate synthase (AS) provides the glutamine amidotransferase activity which generates ammonia as a substrate that, along with chorismate, is used in the second step, catalyzed by the large alpha subunit of AS (TrpE) to produce anthranilate. In the absence of TrpG, TrpE can synthesize anthranilate directly from chorismate and high concentrations of ammonia.1 Publication

    Catalytic activityi

    Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.

    Cofactori

    Binds 1 magnesium ion per subunit.By similarity

    Enzyme regulationi

    Cooperatively feedback inhibited by tryptophan.1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei31 – 311TryptophanBy similarity
    Metal bindingi269 – 2691MagnesiumBy similarity
    Binding sitei357 – 3571SubstrateBy similarity
    Binding sitei377 – 3771SubstrateBy similarity
    Binding sitei393 – 3931Substrate; via amide nitrogenBy similarity
    Metal bindingi406 – 4061MagnesiumBy similarity

    GO - Molecular functioni

    1. anthranilate synthase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. tryptophan biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-3603.
    SSOL273057:GCH2-849-MONOMER.
    UniPathwayiUPA00035; UER00040.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Anthranilate synthase component 1 (EC:4.1.3.27)
    Short name:
    AS
    Short name:
    ASI
    Gene namesi
    Name:trpE
    Ordered Locus Names:SSO0893
    OrganismiSulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
    Taxonomic identifieri273057 [NCBI]
    Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
    ProteomesiUP000001974: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 421421Anthranilate synthase component 1PRO_0000154132Add
    BLAST

    Interactioni

    Subunit structurei

    Heterotetramer consisting of two non-identical subunits: a beta subunit (TrpG) and a large alpha subunit (TrpE).1 Publication

    Protein-protein interaction databases

    DIPiDIP-6202N.
    IntActiQ06128. 1 interaction.
    MINTiMINT-102245.
    STRINGi273057.SSO0893.

    Structurei

    Secondary structure

    421
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi1 – 55
    Helixi6 – 83
    Helixi12 – 2211
    Beta strandi24 – 307
    Beta strandi41 – 466
    Beta strandi52 – 543
    Helixi58 – 636
    Turni64 – 685
    Beta strandi75 – 773
    Beta strandi79 – 868
    Helixi88 – 936
    Beta strandi103 – 1053
    Beta strandi109 – 1135
    Beta strandi116 – 1227
    Helixi123 – 1253
    Beta strandi127 – 1326
    Beta strandi146 – 15510
    Helixi157 – 17317
    Beta strandi177 – 19115
    Helixi194 – 20310
    Beta strandi207 – 2148
    Beta strandi217 – 2248
    Beta strandi226 – 2316
    Beta strandi234 – 2374
    Beta strandi240 – 2456
    Helixi250 – 26112
    Helixi264 – 28219
    Beta strandi291 – 30010
    Beta strandi302 – 31514
    Helixi321 – 3288
    Helixi332 – 3343
    Beta strandi335 – 3384
    Helixi339 – 34911
    Beta strandi350 – 3534
    Turni355 – 3584
    Beta strandi359 – 3657
    Beta strandi370 – 3756
    Beta strandi377 – 3826
    Beta strandi385 – 39410
    Helixi400 – 41718

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1QDLX-ray2.50A1-421[»]
    ProteinModelPortaliQ06128.
    SMRiQ06128. Positions 1-421.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ06128.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni207 – 2093Tryptophan bindingBy similarity
    Regioni242 – 2432Substrate bindingBy similarity
    Regioni391 – 3933Substrate bindingBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0147.
    HOGENOMiHOG000025142.
    KOiK01657.
    OMAiADFAITI.

    Family and domain databases

    Gene3Di3.60.120.10. 1 hit.
    InterProiIPR005801. ADC_synthase.
    IPR019999. Anth_synth_I_like.
    IPR006805. Anth_synth_I_N.
    IPR010116. Anthranilate_synth_I_arc_typ.
    IPR015890. Chorismate-bd_C.
    [Graphical view]
    PfamiPF04715. Anth_synt_I_N. 1 hit.
    PF00425. Chorismate_bind. 1 hit.
    [Graphical view]
    PRINTSiPR00095. ANTSNTHASEI.
    SUPFAMiSSF56322. SSF56322. 1 hit.
    TIGRFAMsiTIGR01820. TrpE-arch. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q06128-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEVHPISEFA SPFEVFKCIE RDFKVAGLLE SIGGPQYKAR YSVIAWSTNG    50
    YLKIHDDPVN ILNGYLKDLK LADIPGLFKG GMIGYISYDA VRFWEKIRDL 100
    KPAAEDWPYA EFFTPDNIII YDHNEGKVYV NADLSSVGGC GDIGEFKVSF 150
    YDESLNKNSY ERIVSESLEY IRSGYIFQVV LSRFYRYIFS GDPLRIYYNL 200
    RRINPSPYMF YLKFDEKYLI GSSPELLFRV QDNIVETYPI AGTRPRGADQ 250
    EEDLKLELEL MNSEKDKAEH LMLVDLARND LGKVCVPGTV KVPELMYVEK 300
    YSHVQHIVSK VIGTLKKKYN ALNVLSATFP AGTVSGAPKP MAMNIIETLE 350
    EYKRGPYAGA VGFISADGNA EFAIAIRTAF LNKELLRIHA GAGIVYDSNP 400
    ESEYFETEHK LKALKTAIGV R 421
    Length:421
    Mass (Da):47,652
    Last modified:June 20, 2001 - v2
    Checksum:iBD49BD7771ECC6EE
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti337 – 3371A → R in AAA73379. (PubMed:8416906)Curated
    Sequence conflicti337 – 3371A → R in CAA90311. (PubMed:9016772)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M98048 Genomic DNA. Translation: AAA73379.1.
    Z50014 Genomic DNA. Translation: CAA90311.1.
    AE006641 Genomic DNA. Translation: AAK41175.1.
    PIRiH90239.
    JC5323.
    RefSeqiNP_342385.1. NC_002754.1.
    WP_009992309.1. NC_002754.1.

    Genome annotation databases

    EnsemblBacteriaiAAK41175; AAK41175; SSO0893.
    GeneIDi1455142.
    KEGGisso:SSO0893.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M98048 Genomic DNA. Translation: AAA73379.1 .
    Z50014 Genomic DNA. Translation: CAA90311.1 .
    AE006641 Genomic DNA. Translation: AAK41175.1 .
    PIRi H90239.
    JC5323.
    RefSeqi NP_342385.1. NC_002754.1.
    WP_009992309.1. NC_002754.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1QDL X-ray 2.50 A 1-421 [» ]
    ProteinModelPortali Q06128.
    SMRi Q06128. Positions 1-421.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-6202N.
    IntActi Q06128. 1 interaction.
    MINTi MINT-102245.
    STRINGi 273057.SSO0893.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAK41175 ; AAK41175 ; SSO0893 .
    GeneIDi 1455142.
    KEGGi sso:SSO0893.

    Phylogenomic databases

    eggNOGi COG0147.
    HOGENOMi HOG000025142.
    KOi K01657.
    OMAi ADFAITI.

    Enzyme and pathway databases

    UniPathwayi UPA00035 ; UER00040 .
    BioCyci MetaCyc:MONOMER-3603.
    SSOL273057:GCH2-849-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei Q06128.

    Family and domain databases

    Gene3Di 3.60.120.10. 1 hit.
    InterProi IPR005801. ADC_synthase.
    IPR019999. Anth_synth_I_like.
    IPR006805. Anth_synth_I_N.
    IPR010116. Anthranilate_synth_I_arc_typ.
    IPR015890. Chorismate-bd_C.
    [Graphical view ]
    Pfami PF04715. Anth_synt_I_N. 1 hit.
    PF00425. Chorismate_bind. 1 hit.
    [Graphical view ]
    PRINTSi PR00095. ANTSNTHASEI.
    SUPFAMi SSF56322. SSF56322. 1 hit.
    TIGRFAMsi TIGR01820. TrpE-arch. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Tryptophan biosynthesis genes trpEGC in the thermoacidophilic archaebacterium Sulfolobus solfataricus."
      Tutino M.L., Scarano G., Marino G., Sannia G., Cubellis M.V.
      J. Bacteriol. 175:299-302(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: DSM 5833 / MT-4.
    2. "Expression of Sulfolobus solfataricus trpE and trpG genes in E. coli."
      Tutino M.L., Tosco A., Marino G., Sannia G.
      Biochem. Biophys. Res. Commun. 230:306-310(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: DSM 5833 / MT-4.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
    4. "The crystal structure of anthranilate synthase from Sulfolobus solfataricus: functional implications."
      Knoechel T., Ivens A., Hester G., Gonzalez A., Bauerle R., Wilmanns M., Kirschner K., Jansonius J.N.
      Proc. Natl. Acad. Sci. U.S.A. 96:9479-9484(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS), FUNCTION, ENZYME REGULATION, SUBUNIT.

    Entry informationi

    Entry nameiTRPE_SULSO
    AccessioniPrimary (citable) accession number: Q06128
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: June 20, 2001
    Last modified: October 1, 2014
    This is version 118 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Allosteric enzyme, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3