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Q06128

- TRPE_SULSO

UniProt

Q06128 - TRPE_SULSO

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Protein

Anthranilate synthase component 1

Gene
trpE, SSO0893
Organism
Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Part of a heterotetrameric complex that catalyzes the two-step biosynthesis of anthranilate, an intermediate in the biosynthesis of L-tryptophan. In the first step, the glutamine-binding beta subunit (TrpG) of anthranilate synthase (AS) provides the glutamine amidotransferase activity which generates ammonia as a substrate that, along with chorismate, is used in the second step, catalyzed by the large alpha subunit of AS (TrpE) to produce anthranilate. In the absence of TrpG, TrpE can synthesize anthranilate directly from chorismate and high concentrations of ammonia.1 Publication

Catalytic activityi

Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.

Cofactori

Binds 1 magnesium ion per subunit By similarity.

Enzyme regulationi

Cooperatively feedback inhibited by tryptophan.1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei31 – 311Tryptophan By similarity
Metal bindingi269 – 2691Magnesium By similarity
Binding sitei357 – 3571Substrate By similarity
Binding sitei377 – 3771Substrate By similarity
Binding sitei393 – 3931Substrate; via amide nitrogen By similarity
Metal bindingi406 – 4061Magnesium By similarity

GO - Molecular functioni

  1. anthranilate synthase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. tryptophan biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-3603.
SSOL273057:GCH2-849-MONOMER.
UniPathwayiUPA00035; UER00040.

Names & Taxonomyi

Protein namesi
Recommended name:
Anthranilate synthase component 1 (EC:4.1.3.27)
Short name:
AS
Short name:
ASI
Gene namesi
Name:trpE
Ordered Locus Names:SSO0893
OrganismiSulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Taxonomic identifieri273057 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
ProteomesiUP000001974: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 421421Anthranilate synthase component 1PRO_0000154132Add
BLAST

Interactioni

Subunit structurei

Heterotetramer consisting of two non-identical subunits: a beta subunit (TrpG) and a large alpha subunit (TrpE).1 Publication

Protein-protein interaction databases

DIPiDIP-6202N.
IntActiQ06128. 1 interaction.
MINTiMINT-102245.
STRINGi273057.SSO0893.

Structurei

Secondary structure

421
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi1 – 55
Helixi6 – 83
Helixi12 – 2211
Beta strandi24 – 307
Beta strandi41 – 466
Beta strandi52 – 543
Helixi58 – 636
Turni64 – 685
Beta strandi75 – 773
Beta strandi79 – 868
Helixi88 – 936
Beta strandi103 – 1053
Beta strandi109 – 1135
Beta strandi116 – 1227
Helixi123 – 1253
Beta strandi127 – 1326
Beta strandi146 – 15510
Helixi157 – 17317
Beta strandi177 – 19115
Helixi194 – 20310
Beta strandi207 – 2148
Beta strandi217 – 2248
Beta strandi226 – 2316
Beta strandi234 – 2374
Beta strandi240 – 2456
Helixi250 – 26112
Helixi264 – 28219
Beta strandi291 – 30010
Beta strandi302 – 31514
Helixi321 – 3288
Helixi332 – 3343
Beta strandi335 – 3384
Helixi339 – 34911
Beta strandi350 – 3534
Turni355 – 3584
Beta strandi359 – 3657
Beta strandi370 – 3756
Beta strandi377 – 3826
Beta strandi385 – 39410
Helixi400 – 41718

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1QDLX-ray2.50A1-421[»]
ProteinModelPortaliQ06128.
SMRiQ06128. Positions 1-421.

Miscellaneous databases

EvolutionaryTraceiQ06128.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni207 – 2093Tryptophan binding By similarity
Regioni242 – 2432Substrate binding By similarity
Regioni391 – 3933Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0147.
HOGENOMiHOG000025142.
KOiK01657.
OMAiADFAITI.

Family and domain databases

Gene3Di3.60.120.10. 1 hit.
InterProiIPR005801. ADC_synthase.
IPR019999. Anth_synth_I_like.
IPR006805. Anth_synth_I_N.
IPR010116. Anthranilate_synth_I_arc_typ.
IPR015890. Chorismate-bd_C.
[Graphical view]
PfamiPF04715. Anth_synt_I_N. 1 hit.
PF00425. Chorismate_bind. 1 hit.
[Graphical view]
PRINTSiPR00095. ANTSNTHASEI.
SUPFAMiSSF56322. SSF56322. 1 hit.
TIGRFAMsiTIGR01820. TrpE-arch. 1 hit.

Sequencei

Sequence statusi: Complete.

Q06128-1 [UniParc]FASTAAdd to Basket

« Hide

MEVHPISEFA SPFEVFKCIE RDFKVAGLLE SIGGPQYKAR YSVIAWSTNG    50
YLKIHDDPVN ILNGYLKDLK LADIPGLFKG GMIGYISYDA VRFWEKIRDL 100
KPAAEDWPYA EFFTPDNIII YDHNEGKVYV NADLSSVGGC GDIGEFKVSF 150
YDESLNKNSY ERIVSESLEY IRSGYIFQVV LSRFYRYIFS GDPLRIYYNL 200
RRINPSPYMF YLKFDEKYLI GSSPELLFRV QDNIVETYPI AGTRPRGADQ 250
EEDLKLELEL MNSEKDKAEH LMLVDLARND LGKVCVPGTV KVPELMYVEK 300
YSHVQHIVSK VIGTLKKKYN ALNVLSATFP AGTVSGAPKP MAMNIIETLE 350
EYKRGPYAGA VGFISADGNA EFAIAIRTAF LNKELLRIHA GAGIVYDSNP 400
ESEYFETEHK LKALKTAIGV R 421
Length:421
Mass (Da):47,652
Last modified:June 20, 2001 - v2
Checksum:iBD49BD7771ECC6EE
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti337 – 3371A → R in AAA73379. 1 Publication
Sequence conflicti337 – 3371A → R in CAA90311. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M98048 Genomic DNA. Translation: AAA73379.1.
Z50014 Genomic DNA. Translation: CAA90311.1.
AE006641 Genomic DNA. Translation: AAK41175.1.
PIRiH90239.
JC5323.
RefSeqiNP_342385.1. NC_002754.1.
WP_009992309.1. NC_002754.1.

Genome annotation databases

EnsemblBacteriaiAAK41175; AAK41175; SSO0893.
GeneIDi1455142.
KEGGisso:SSO0893.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M98048 Genomic DNA. Translation: AAA73379.1 .
Z50014 Genomic DNA. Translation: CAA90311.1 .
AE006641 Genomic DNA. Translation: AAK41175.1 .
PIRi H90239.
JC5323.
RefSeqi NP_342385.1. NC_002754.1.
WP_009992309.1. NC_002754.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1QDL X-ray 2.50 A 1-421 [» ]
ProteinModelPortali Q06128.
SMRi Q06128. Positions 1-421.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-6202N.
IntActi Q06128. 1 interaction.
MINTi MINT-102245.
STRINGi 273057.SSO0893.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAK41175 ; AAK41175 ; SSO0893 .
GeneIDi 1455142.
KEGGi sso:SSO0893.

Phylogenomic databases

eggNOGi COG0147.
HOGENOMi HOG000025142.
KOi K01657.
OMAi ADFAITI.

Enzyme and pathway databases

UniPathwayi UPA00035 ; UER00040 .
BioCyci MetaCyc:MONOMER-3603.
SSOL273057:GCH2-849-MONOMER.

Miscellaneous databases

EvolutionaryTracei Q06128.

Family and domain databases

Gene3Di 3.60.120.10. 1 hit.
InterProi IPR005801. ADC_synthase.
IPR019999. Anth_synth_I_like.
IPR006805. Anth_synth_I_N.
IPR010116. Anthranilate_synth_I_arc_typ.
IPR015890. Chorismate-bd_C.
[Graphical view ]
Pfami PF04715. Anth_synt_I_N. 1 hit.
PF00425. Chorismate_bind. 1 hit.
[Graphical view ]
PRINTSi PR00095. ANTSNTHASEI.
SUPFAMi SSF56322. SSF56322. 1 hit.
TIGRFAMsi TIGR01820. TrpE-arch. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Tryptophan biosynthesis genes trpEGC in the thermoacidophilic archaebacterium Sulfolobus solfataricus."
    Tutino M.L., Scarano G., Marino G., Sannia G., Cubellis M.V.
    J. Bacteriol. 175:299-302(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: DSM 5833 / MT-4.
  2. "Expression of Sulfolobus solfataricus trpE and trpG genes in E. coli."
    Tutino M.L., Tosco A., Marino G., Sannia G.
    Biochem. Biophys. Res. Commun. 230:306-310(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: DSM 5833 / MT-4.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
  4. "The crystal structure of anthranilate synthase from Sulfolobus solfataricus: functional implications."
    Knoechel T., Ivens A., Hester G., Gonzalez A., Bauerle R., Wilmanns M., Kirschner K., Jansonius J.N.
    Proc. Natl. Acad. Sci. U.S.A. 96:9479-9484(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS), FUNCTION, ENZYME REGULATION, SUBUNIT.

Entry informationi

Entry nameiTRPE_SULSO
AccessioniPrimary (citable) accession number: Q06128
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: June 20, 2001
Last modified: September 3, 2014
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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