Q06121 (TRPC_SULSO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Indole-3-glycerol phosphate synthase
Short name=IGPS
EC=4.1.1.48

Gene names

Name:	trpC
Ordered Locus Names:	SSO0895

Organism

Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)

Taxonomic identifier

273057 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus

Protein attributes

Sequence length	248 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate = 1-C-(3-indolyl)-glycerol 3-phosphate + CO2 + H2O. HAMAP MF_00134_A
Pathway	Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 4/5. HAMAP MF_00134_A
Subunit structure	Monomer.
Sequence similarities	Belongs to the TrpC family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis Tryptophan biosynthesis
Molecular function	Decarboxylase Lyase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	tryptophan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	indole-3-glycerol-phosphate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 248

248

Indole-3-glycerol phosphate synthase HAMAP MF_00134_A

PRO_0000154302

Secondary structure

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248

Helix Strand Turn

Details...

Helix

7 – 17

11

Helix

33 – 42

10

Beta strand

48 – 52

5

Helix

66 – 73

8

Turn

74 – 76

3

Beta strand

78 – 83

6

Turn

87 – 89

3

Helix

93 – 102

10

Beta strand

107 – 111

5

Helix

116 – 125

10

Beta strand

128 – 133

6

Helix

134 – 136

3

Helix

139 – 150

12

Turn

151 – 153

3

Beta strand

157 – 160

4

Helix

163 – 171

9

Beta strand

175 – 182

8

Turn

184 – 186

3

Helix

191 – 200

10

Beta strand

205 – 211

7

Helix

216 – 224

9

Beta strand

229 – 232

4

Helix

234 – 238

5

Helix

242 – 246

5

Sequences

	Sequence		Length	Mass (Da)
	Q06121 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 919E2AEEE68DDF65	FASTA	248	28,588
	10 20 30 40 50 60 MPRYLKGWLK DVVQLSLRRP SFRASRQRPI ISLNERILEF NKRNITAIIA EYKRKSPSGL 70 80 90 100 110 120 DVERDPIEYS KFMERYAVGL SILTEEKYFN GSYETLRKIA SSVSIPILMK DFIVKESQID 130 140 150 160 170 180 DAYNLGADTV LLIVKILTER ELESLLEYAR SYGMEPLIEI NDENDLDIAL RIGARFIGIN 190 200 210 220 230 240 SRDLETLEIN KENQRKLISM IPSNVVKVAE SGISERNEIE ELRKLGVNAF LIGSSLMRNP EKIKEFIL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Tryptophan biosynthesis genes trpEGC in the thermoacidophilic archaebacterium Sulfolobus solfataricus." Tutino M.L., Scarano G., Marino G., Sannia G., Cubellis M.V. J. Bacteriol. 175:299-302(1993) [PubMed: 8416906] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DSM 5833 / MT-4.
[2]	"The tryptophan operon in Sulfolobus solfataricus." Tutino M.L., Cubellis M., Sannia G., Marino G. Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DSM 5833 / MT-4.
[3]	"The complete genome of the crenarchaeon Sulfolobus solfataricus P2." She Q., Singh R.K., Confalonieri F., Zivanovic Y., Allard G., Awayez M.J., Chan-Weiher C.C.-Y., Clausen I.G., Curtis B.A., De Moors A., Erauso G., Fletcher C., Gordon P.M.K., Heikamp-de Jong I., Jeffries A.C., Kozera C.J., Medina N., Peng X. , Thi-Ngoc H.P., Redder P., Schenk M.E., Theriault C., Tolstrup N., Charlebois R.L., Doolittle W.F., Duguet M., Gaasterland T., Garrett R.A., Ragan M.A., Sensen C.W., Van der Oost J. Proc. Natl. Acad. Sci. U.S.A. 98:7835-7840(2001) [PubMed: 11427726] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
[4]	"2.0-A structure of indole-3-glycerol phosphate synthase from the hyperthermophile Sulfolobus solfataricus: possible determinants of protein stability." Hennig M., Darimont B., Kirschner K., Jansonius J.N. Structure 3:1295-1306(1995) [PubMed: 8747456] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
[5]	"The crystal structure of indole-3-glycerol phosphate synthase from the hyperthermophilic archaeon Sulfolobus solfataricus in three different crystal forms: effects of ionic strength." Knoechel T.R., Hennig M., Merz A., Darimont B., Kirschner K., Jansonius J.N. J. Mol. Biol. 262:502-515(1996) [PubMed: 8893859] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
[6]	"The catalytic mechanism of indole-3-glycerolphosphate synthase: crystal structure of substrate, product and substrate analogue bound to the enzyme from Sulfolobus solfataricus." Hennig M., Darimont B., Kirschner K., Jansonius J.N. Submitted (FEB-1998) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M98048 Genomic DNA. Translation: AAA73381.1.
Z50014 Genomic DNA. Translation: CAA90313.1.
AE006641 Genomic DNA. Translation: AAK41177.1.

PIR

C40635.
S50179.

RefSeq

NP_342387.1. NC_002754.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1A53	X-ray	2.00	A	2-248	[»]
1IGS	X-ray	2.00	A	1-248	[»]
1JUK	X-ray	2.50	A	1-248	[»]
1JUL	X-ray	2.00	A	1-248	[»]
1LBF	X-ray	2.05	A	2-248	[»]
1LBL	X-ray	2.40	A	2-248	[»]
2C3Z	X-ray	2.80	A	27-248	[»]
3NYZ	X-ray	1.51	A/B	1-248	[»]
3NZ1	X-ray	1.56	A	1-248	[»]
3TC6	X-ray	1.60	A	1-245	[»]
3TC7	X-ray	1.50	A	1-245	[»]

ProteinModelPortal

Q06121.

SMR

Q06121. Positions 2-248.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

1455144.

GenomeReviews

Gene locus SSO0895 in contig AE006641_GR.

KEGG

sso:SSO0895.

NMPDR

fig|273057.1.peg.810.

Phylogenomic databases

HOGENOM

HBG540956.

OMA

FETDINT.

ProtClustDB

PRK00278.

Enzyme and pathway databases

BioCyc

MetaCyc:MONOMER-3602.
SSOL273057:SSO0895-MONOMER.

Family and domain databases

HAMAP

MF_00134_A. IGPS_A.
[Tree]

InterPro

IPR013785. Aldolase_TIM.
IPR013798. Indole-3-glycerol_P_synth.
IPR001468. Indole-3-GlycerolPSynthase_CS.
IPR011060. RibuloseP-bd_barrel.
[Graphical view]

Gene3D

G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.

KO

K01609.

Pfam

PF00218. IGPS. 1 hit.
[Graphical view]

SUPFAM

SSF51366. RibP_bind_barrel. 1 hit.

PROSITE

PS00614. IGPS. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

TRPC_SULSO

Accession

Primary (citable) accession number: Q06121
Secondary accession number(s): P50385

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	November 16, 2011
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program