Q06119 (KDGL_RHIME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Diacylglycerol kinase
Short name=DAGK
EC=2.7.1.107

Alternative name(s):
Diglyceride kinase
Short name=DGK

Gene names

Name:	dgkA
Ordered Locus Names:	R01883
ORF Names:	SMc04213

Organism

Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti) [Complete proteome] [HAMAP]

Taxonomic identifier

266834 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium

Protein attributes

Sequence length	137 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Recycling of diacylglycerol produced during cyclic beta-1,2-glucan biosynthesis.
Catalytic activity	ATP + 1,2-diacylglycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the bacterial diacylglycerol kinase family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW diacylglycerol kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 137	137	Diacylglycerol kinase		PRO_0000195267
Regions
	Topological domain	1 – 46	46	Cytoplasmic Potential
	Transmembrane	47 – 62	16	Helical; Potential
	Transmembrane	66 – 82	17	Helical; Potential
	Topological domain	83 – 109	27	Cytoplasmic Potential
	Transmembrane	110 – 133	24	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	Q06119 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 0BE65269F1145BC5	FASTA	137	14,892
	10 20 30 40 50 60 MDAKNTTSRN GTAAPVHKHR GIRHLFAAAS YSFGGAKRLI GEAAFRHELI AFAVAMVAFM 70 80 90 100 110 120 IVGANLFQYV AMAILFLLMM AFEAINTAIE EIVDRVSPEI SEMGRNAKDL GSFACLCLIL 130 ANAAYALYVI FLARFLG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of the diacylglycerol kinase structural gene of Rhizobium meliloti 1021." Miller K.J., McKinstry M.W., Hunt W.P., Nixon B.T. Mol. Plant Microbe Interact. 5:363-371(1992) [PubMed: 1335301] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 1021.
[2]	"Analysis of the chromosome sequence of the legume symbiont Sinorhizobium meliloti strain 1021." Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J., Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S., Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D. , Pohl T., Portetelle D., Puehler A., Purnelle B., Ramsperger U., Renard C., Thebault P., Vandenbol M., Weidner S., Galibert F. Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001) [PubMed: 11481430] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021.
[3]	"The composite genome of the legume symbiont Sinorhizobium meliloti." Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F., Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G., Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P., Cowie A. , Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S., Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I., Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S., Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C., Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R., Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H., Wong K., Yeh K.-C., Batut J. Science 293:668-672(2001) [PubMed: 11474104] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M94085 Genomic DNA. Translation: AAA26257.1. AL591688 Genomic DNA. Translation: CAC46462.1.
PIR	S27659.
RefSeq	NP_385989.1. NC_003047.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1233548.
GenomeReviews	Gene locus R01883 in contig AL591688_GR.
KEGG	sme:SMc04213.
NMPDR	fig|266834.1.peg.3177.
PATRIC	23633175. VBISinMel96828_3326.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG729542.
OMA	VGKHAKD.
ProtClustDB	CLSK2484729.
Enzyme and pathway databases
BioCyc	SMEL266834:SMC04213-MONOMER.
Family and domain databases
InterPro	IPR000829. Diacylglycerol_kinase_prok. [Graphical view]
KO	K00901.
Pfam	PF01219. DAGK_prokar. 1 hit. [Graphical view]
PROSITE	PS01069. DAGK_PROKAR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KDGL_RHIME

Accession

Primary (citable) accession number: Q06119

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	January 25, 2012
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program