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Q06115 (CBH_LACPL) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Choloylglycine hydrolase

EC=3.5.1.24
Alternative name(s):
Bile salt hydrolase
Conjugated bile acid hydrolase
Short name=CBAH
Gene names
Name:cbh
Synonyms:bsh
Ordered Locus Names:lp_3536
OrganismLactobacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1) [Reference proteome] [HAMAP]
Taxonomic identifier220668 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

Protein attributes

Sequence length324 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

The enzyme catalyzes the degradation of conjugated bile acids in the mammalian gut By similarity.

Catalytic activity

3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholan-24-oylglycine + H2O = 3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholanate + glycine.

Sequence similarities

Belongs to the peptidase C59 family.

Ontologies

Keywords
   Molecular functionHydrolase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Molecular_functioncholoylglycine hydrolase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 324323Choloylglycine hydrolase
PRO_0000073020

Sites

Active site21 By similarity

Experimental info

Sequence conflict2361S → P in AAB24746. Ref.1
Sequence conflict2361S → P in AAA25233. Ref.1
Sequence conflict2981S → N in AAB24746. Ref.1
Sequence conflict2981S → N in AAA25233. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q06115 [UniParc].

Last modified January 23, 2007. Version 4.
Checksum: B5E53F74AD742347

FASTA32437,042
        10         20         30         40         50         60 
MCTAITYQSY NNYFGRNFDY EISYNEMVTI TPRKYPLVFR KVENLDHHYA IIGITADVES 

        70         80         90        100        110        120 
YPLYYDAMNE KGLCIAGLNF AGYADYKKYD ADKVNITPFE LIPWLLGQFS SVREVKKNIQ 

       130        140        150        160        170        180 
KLNLVNINFS EQLPLSPLHW LVADKQESIV IESVKEGLKI YDNPVGVLTN NPNFDYQLFN 

       190        200        210        220        230        240 
LNNYRALSNS TPQNSFSEKV DLDSYSRGMG GLGLPGDLSS MSRFVRAAFT KLNSLSMQTE 

       250        260        270        280        290        300 
SGSVSQFFHI LGSVEQQKGL CEVTDGKYEY TIYSSCCDMD KGVYYYRTYD NSQINSVSLN 

       310        320 
HEHLDTTELI SYPLRSEAQY YAVN 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and expression of a conjugated bile acid hydrolase gene from Lactobacillus plantarum by using a direct plate assay."
Christiaens H., Leer R.J., Pouwels P.H., Verstraete W.
Appl. Environ. Microbiol. 58:3792-3798(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: DSM 4229 / 80.
[2]"Complete genome sequence of Lactobacillus plantarum WCFS1."
Kleerebezem M., Boekhorst J., van Kranenburg R., Molenaar D., Kuipers O.P., Leer R., Tarchini R., Peters S.A., Sandbrink H.M., Fiers M.W.E.J., Stiekema W., Klein Lankhorst R.M., Bron P.A., Hoffer S.M., Nierop Groot M.N., Kerkhoven R., De Vries M., Ursing B., De Vos W.M., Siezen R.J.
Proc. Natl. Acad. Sci. U.S.A. 100:1990-1995(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-793 / NCIMB 8826 / WCFS1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
S51638 Genomic DNA. Translation: AAB24746.1.
M96175 Genomic DNA. Translation: AAA25233.1.
AL935263 Genomic DNA. Translation: CCC80500.1.
PIRA48953.
RefSeqYP_004891014.1. NC_004567.2.

3D structure databases

ProteinModelPortalQ06115.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING220668.lp_3536.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCCC80500; CCC80500; lp_3536.
GeneID1062382.
KEGGlpl:lp_3536.
PATRIC22252698. VBILacPla27411_2947.

Phylogenomic databases

eggNOGCOG3049.
HOGENOMHOG000039938.
KOK01442.
OMAASFEFIP.
ProtClustDBCLSK809444.

Family and domain databases

Gene3D3.60.60.10. 1 hit.
InterProIPR003199. Chologlycine_hydro.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCBH_LACPL
AccessionPrimary (citable) accession number: Q06115
Secondary accession number(s): F9UUJ8
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 82 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries