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Protein

Choloylglycine hydrolase

Gene

cbh

Organism
Lactobacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

The enzyme catalyzes the degradation of conjugated bile acids in the mammalian gut.By similarity

Catalytic activityi

3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholan-24-oylglycine + H2O = 3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholanate + glycine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21By similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BRENDAi3.5.1.24. 2849.

Names & Taxonomyi

Protein namesi
Recommended name:
Choloylglycine hydrolase (EC:3.5.1.24)
Alternative name(s):
Bile salt hydrolase
Conjugated bile acid hydrolase
Short name:
CBAH
Gene namesi
Name:cbh
Synonyms:bsh
Ordered Locus Names:lp_3536
OrganismiLactobacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1)
Taxonomic identifieri220668 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
ProteomesiUP000000432 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 324323Choloylglycine hydrolasePRO_0000073020Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi220668.lp_3536.

Structurei

3D structure databases

ProteinModelPortaliQ06115.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase C59 family.Curated

Phylogenomic databases

eggNOGiCOG3049.
HOGENOMiHOG000039938.
KOiK01442.
OMAiASFEFIP.

Family and domain databases

Gene3Di3.60.60.10. 1 hit.
InterProiIPR029132. CBAH/NAAA_C.
IPR003199. Chologlycine_hydro/PeptC59.
IPR029055. Ntn_hydrolases_N.
[Graphical view]
PfamiPF02275. CBAH. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q06115-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCTAITYQSY NNYFGRNFDY EISYNEMVTI TPRKYPLVFR KVENLDHHYA
60 70 80 90 100
IIGITADVES YPLYYDAMNE KGLCIAGLNF AGYADYKKYD ADKVNITPFE
110 120 130 140 150
LIPWLLGQFS SVREVKKNIQ KLNLVNINFS EQLPLSPLHW LVADKQESIV
160 170 180 190 200
IESVKEGLKI YDNPVGVLTN NPNFDYQLFN LNNYRALSNS TPQNSFSEKV
210 220 230 240 250
DLDSYSRGMG GLGLPGDLSS MSRFVRAAFT KLNSLSMQTE SGSVSQFFHI
260 270 280 290 300
LGSVEQQKGL CEVTDGKYEY TIYSSCCDMD KGVYYYRTYD NSQINSVSLN
310 320
HEHLDTTELI SYPLRSEAQY YAVN
Length:324
Mass (Da):37,042
Last modified:January 23, 2007 - v4
Checksum:iB5E53F74AD742347
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti236 – 2361S → P in AAB24746 (PubMed:1476424).Curated
Sequence conflicti236 – 2361S → P in AAA25233 (PubMed:1476424).Curated
Sequence conflicti298 – 2981S → N in AAB24746 (PubMed:1476424).Curated
Sequence conflicti298 – 2981S → N in AAA25233 (PubMed:1476424).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S51638 Genomic DNA. Translation: AAB24746.1.
M96175 Genomic DNA. Translation: AAA25233.1.
AL935263 Genomic DNA. Translation: CCC80500.1.
PIRiA48953.
RefSeqiWP_011102211.1. NC_004567.2.
YP_004891014.1. NC_004567.2.

Genome annotation databases

EnsemblBacteriaiCCC80500; CCC80500; lp_3536.
GeneIDi1062382.
KEGGilpl:lp_3536.
PATRICi22252698. VBILacPla27411_2947.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S51638 Genomic DNA. Translation: AAB24746.1.
M96175 Genomic DNA. Translation: AAA25233.1.
AL935263 Genomic DNA. Translation: CCC80500.1.
PIRiA48953.
RefSeqiWP_011102211.1. NC_004567.2.
YP_004891014.1. NC_004567.2.

3D structure databases

ProteinModelPortaliQ06115.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi220668.lp_3536.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCCC80500; CCC80500; lp_3536.
GeneIDi1062382.
KEGGilpl:lp_3536.
PATRICi22252698. VBILacPla27411_2947.

Phylogenomic databases

eggNOGiCOG3049.
HOGENOMiHOG000039938.
KOiK01442.
OMAiASFEFIP.

Enzyme and pathway databases

BRENDAi3.5.1.24. 2849.

Family and domain databases

Gene3Di3.60.60.10. 1 hit.
InterProiIPR029132. CBAH/NAAA_C.
IPR003199. Chologlycine_hydro/PeptC59.
IPR029055. Ntn_hydrolases_N.
[Graphical view]
PfamiPF02275. CBAH. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and expression of a conjugated bile acid hydrolase gene from Lactobacillus plantarum by using a direct plate assay."
    Christiaens H., Leer R.J., Pouwels P.H., Verstraete W.
    Appl. Environ. Microbiol. 58:3792-3798(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: DSM 4229 / 80.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-793 / NCIMB 8826 / WCFS1.

Entry informationi

Entry nameiCBH_LACPL
AccessioniPrimary (citable) accession number: Q06115
Secondary accession number(s): F9UUJ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: January 23, 2007
Last modified: April 1, 2015
This is version 91 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.