Regulator of the glycerol channel 1 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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Q06108 (RGC1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 87. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Regulator of the glycerol channel 1

Gene names

Name:	RGC1
Ordered Locus Names:	YPR115W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	1083 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Positive regulator of FPS1 glycerol channel required for the glycerol efflux. Ref.3 Ref.11
Subcellular location	Cytoplasm Ref.4 Ref.6.
Miscellaneous	Present with 300 molecules/cell in log phase SD medium. Does not bind efficiently to phosphoinositides and does not associate with membranes.
Sequence similarities	Belongs to the RGC1 family. Contains 1 PH domain.

Ontologies

Keywords
Biological process	Cell cycle
Cellular component	Cytoplasm
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of glycerol transport Inferred from mutant phenotype Ref.11. Source: SGD
Cellular_component	cytoplasm Inferred from direct assay PubMed 11914276 Ref.4. Source: SGD
Molecular_function	phospholipid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1083

1083

Regulator of the glycerol channel 1

PRO_0000242488

Regions

Domain

495 – 606

112

Amino acid modifications

Modified residue

481

Phosphoserine Ref.8 Ref.9 Ref.10

Modified residue

535

Phosphoserine Ref.10

Modified residue

537

Phosphoserine Ref.10

Modified residue

652

Phosphoserine Ref.7 Ref.10

Modified residue

778

Phosphoserine Ref.10

Modified residue

817

Phosphothreonine Ref.9 Ref.10

Modified residue

854

Phosphoserine Ref.10

Modified residue

866

Phosphoserine Ref.10

Modified residue

875

Phosphoserine Ref.10

Modified residue

915

Phosphoserine Ref.10

Modified residue

918

Phosphoserine Ref.10

Modified residue

966

Phosphoserine Ref.10

Modified residue

975

Phosphoserine Ref.10

Modified residue

1046

Phosphothreonine Ref.10

Modified residue

1047

Phosphotyrosine Ref.10

Modified residue

1048

Phosphoserine Ref.7 Ref.10

Modified residue

1059

Phosphoserine Ref.10

Sequences

Sequence

Length

Mass (Da)

Tools

Q06108 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 28D63D32843BFE08
Show »

FASTA

1,083

120,395

        10         20         30         40         50         60 
MSDYFTFPKQ ENGGISKQPA TPGSTRSSSR NLELPKNYRS FGGSSDELAS MYSADSQYLM 

        70         80         90        100        110        120 
DMIPDSLTLK NEPASGNTQM NGPDGKENKD IKLDEYILPK TDPRSPYYIN MPIPKKLPKS 

       130        140        150        160        170        180 
EGKARAKQKV NRADPSDLDV ENIYETSGEF VREYPTDILI DRFHKWKKIL KSLIAYFREA 

       190        200        210        220        230        240 
AYSQEQIARI NYQMKNAVKF AFLTDLEDET NKLVDPSISK LPTKKPQPVP LAAQKLDSKY 

       250        260        270        280        290        300 
DTDVEQPQSI QSVPSEEVAS ASSGFMKFGS GSIQDIQVIL KKYHLSLGSQ QYKISKEILA 

       310        320        330        340        350        360 
YIIPKLTDLR KDLTTKMKEI KELNGDFKTN IGEHIKITSR LLNKYIASVK LLDEASTSGD 

       370        380        390        400        410        420 
KQGEKLKPKH DPYLLKLQLD LQLKRQLLEE NYLREAFLNL QSAALQLEKI VYSKIQSALQ 

       430        440        450        460        470        480 
RYSALIDSEA RLMIKNLCHE LQQGILSRPP AVEWDNFVSH HPTCLMNLKS TDPPPQPRRL 

       490        500        510        520        530        540 
SDIVYPNMKS PLAKCIRVGY LLKKTESSKS FTKGYFVLTT NYLHEFKSSD FFLDSKSPRS 

       550        560        570        580        590        600 
KNKPVVEQSD ISRVNKDGTN AGSHPSSKGT QDPKLTKRRK GLSSSNLYPI SSLSLNDCSL 

       610        620        630        640        650        660 
KDSTDSTFVL QGYASYHSPE DTCTKESSTT SDLACPTKTL ASNKGKHQRT PSALSMVSVP 

       670        680        690        700        710        720 
KFLKSSSVPK EQKKAKEEAN INKKSICEKR VEWTFKIFSA SLEPTPEESK NFKKWVQDIK 

       730        740        750        760        770        780 
ALTSFNSTQE RSNFIEEKIL KSRNHNNGKS SQRSKNSTYI TPVDSFVNLS EKVTPSSSVT 

       790        800        810        820        830        840 
TLNTRKRANR PRYIDIPKSA NMNAGAMNSV YRSKVNTPAI DENGNLAIVG ETKNSAPQNG 

       850        860        870        880        890        900 
MSYTIRTPCK SPYSPYTGEG MLYNRSADNL MASSSRKASA PGEVPQIAVS NHGDEAIIPA 

       910        920        930        940        950        960 
SAYSDSSHKS SRASSVASIH NQRVDFYPSP LMNLPGVSPS CLALDGNANG YFGIPLNCNS 

       970        980        990       1000       1010       1020 
EARRGSDLSP FEMESPLFEE NRTQNCSGSR KSSACHIPHQ CGPRKEGNDS RLIYGNEKGA 

      1030       1040       1050       1060       1070       1080 
SQSRLTLKEP LTSKGVEAPY SSLKKTYSAE NVPLTSTVSN DKSLHSRKEG STNTVPATSA 


SSK

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. Hani J. Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"The budding index of Saccharomyces cerevisiae deletion strains identifies genes important for cell cycle progression." Zettel M.F., Garza L.R., Cass A.M., Myhre R.A., Haizlip L.A., Osadebe S.N., Sudimack D.W., Pathak R., Stone T.L., Polymenis M. FEMS Microbiol. Lett. 223:253-258(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Genome-wide analysis of membrane targeting by S.cerevisiae pleckstrin homology domains." Yu J.W., Mendrola J.M., Audhya A., Singh S., Keleti D., DeWald D.B., Murray D., Emr S.D., Lemmon M.A. Mol. Cell 13:677-688(2004) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[7]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-652 AND SER-1048, MASS SPECTROMETRY. Strain: YAL6B.
[8]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-481, MASS SPECTROMETRY. Strain: ADR376.
[9]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-481 AND THR-817, MASS SPECTROMETRY.
[10]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-481; SER-535; SER-537; SER-652; SER-778; THR-817; SER-854; SER-866; SER-875; SER-915; SER-918; SER-966; SER-975; THR-1046; TYR-1047; SER-1048 AND SER-1059, MASS SPECTROMETRY.
[11]	"Identification of positive regulators of the yeast fps1 glycerol channel." Beese S.E., Negishi T., Levin D.E. PLoS Genet. 5:E1000738-E1000738(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U32445 Genomic DNA. Translation: AAB68085.1. BK006949 Genomic DNA. Translation: DAA11530.1.
PIR	S59780.
RefSeq	NP_015440.1. NM_001184212.1.
3D structure databases
ProteinModelPortal	Q06108.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1970N.
IntAct	Q06108. 31 interactions.
MINT	MINT-410152.
STRING	4932.YPR115W.
Proteomic databases
PaxDb	Q06108.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YPR115W; YPR115W; YPR115W.
GeneID	856231.
KEGG	sce:YPR115W.
Organism-specific databases
CYGD	YPR115w.
SGD	S000006319. RGC1.
Phylogenomic databases
eggNOG	NOG41793.
GeneTree	ENSGT00390000002928.
OrthoDB	EOG41K2MS.
Enzyme and pathway databases
BioCyc	YEAST:G3O-34254-MONOMER.
Gene expression databases
Genevestigator	Q06108.
GermOnline	YPR115W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	2.30.29.30. 2 hits.
InterPro	IPR011993. PH_like_dom. IPR001849. Pleckstrin_homology. [Graphical view]
Pfam	PF00169. PH. 1 hit. [Graphical view]
SMART	SM00233. PH. 1 hit. [Graphical view]
PROSITE	PS50003. PH_DOMAIN. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	981479.

Entry information

Entry name

RGC1_YEAST

Accession

Primary (citable) accession number: Q06108
Secondary accession number(s): D6W4B4

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	November 1, 1996
Last modified:	May 29, 2013
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XVI

Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents