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Q06108 (RGC1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Regulator of the glycerol channel 1
Gene names
Name:RGC1
Ordered Locus Names:YPR115W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length1083 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Positive regulator of FPS1 glycerol channel required for the glycerol efflux. Ref.3 Ref.9

Subcellular location

Cytoplasm Ref.4 Ref.6.

Miscellaneous

Present with 300 molecules/cell in log phase SD medium.

Does not bind efficiently to phosphoinositides and does not associate with membranes.

Sequence similarities

Belongs to the RGC1 family.

Contains 1 PH domain.

Ontologies

Keywords
   Biological processCell cycle
   Cellular componentCytoplasm
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcell cycle

Inferred from electronic annotation. Source: UniProtKB-KW

positive regulation of glycerol transport

Inferred from mutant phenotype Ref.9. Source: SGD

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10831083Regulator of the glycerol channel 1
PRO_0000242488

Regions

Domain495 – 606112PH

Amino acid modifications

Modified residue1361Phosphoserine Ref.10
Modified residue2491Phosphoserine Ref.10
Modified residue2521Phosphoserine Ref.10
Modified residue4811Phosphoserine Ref.7 Ref.8 Ref.10
Modified residue5371Phosphoserine Ref.10
Modified residue6521Phosphoserine Ref.8 Ref.10
Modified residue7651Phosphoserine Ref.10
Modified residue8131Phosphoserine Ref.10
Modified residue8171Phosphothreonine Ref.8 Ref.10
Modified residue8571Phosphothreonine Ref.10
Modified residue8661Phosphoserine Ref.8 Ref.10
Modified residue8791Phosphoserine Ref.10
Modified residue9181Phosphoserine Ref.8
Modified residue9661Phosphoserine Ref.10
Modified residue9691Phosphoserine Ref.10
Modified residue9751Phosphoserine Ref.10
Modified residue10591Phosphoserine Ref.8 Ref.10
Modified residue10811Phosphoserine Ref.10
Modified residue10821Phosphoserine Ref.10

Sequences

Sequence LengthMass (Da)Tools
Q06108 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 28D63D32843BFE08

FASTA1,083120,395
        10         20         30         40         50         60 
MSDYFTFPKQ ENGGISKQPA TPGSTRSSSR NLELPKNYRS FGGSSDELAS MYSADSQYLM 

        70         80         90        100        110        120 
DMIPDSLTLK NEPASGNTQM NGPDGKENKD IKLDEYILPK TDPRSPYYIN MPIPKKLPKS 

       130        140        150        160        170        180 
EGKARAKQKV NRADPSDLDV ENIYETSGEF VREYPTDILI DRFHKWKKIL KSLIAYFREA 

       190        200        210        220        230        240 
AYSQEQIARI NYQMKNAVKF AFLTDLEDET NKLVDPSISK LPTKKPQPVP LAAQKLDSKY 

       250        260        270        280        290        300 
DTDVEQPQSI QSVPSEEVAS ASSGFMKFGS GSIQDIQVIL KKYHLSLGSQ QYKISKEILA 

       310        320        330        340        350        360 
YIIPKLTDLR KDLTTKMKEI KELNGDFKTN IGEHIKITSR LLNKYIASVK LLDEASTSGD 

       370        380        390        400        410        420 
KQGEKLKPKH DPYLLKLQLD LQLKRQLLEE NYLREAFLNL QSAALQLEKI VYSKIQSALQ 

       430        440        450        460        470        480 
RYSALIDSEA RLMIKNLCHE LQQGILSRPP AVEWDNFVSH HPTCLMNLKS TDPPPQPRRL 

       490        500        510        520        530        540 
SDIVYPNMKS PLAKCIRVGY LLKKTESSKS FTKGYFVLTT NYLHEFKSSD FFLDSKSPRS 

       550        560        570        580        590        600 
KNKPVVEQSD ISRVNKDGTN AGSHPSSKGT QDPKLTKRRK GLSSSNLYPI SSLSLNDCSL 

       610        620        630        640        650        660 
KDSTDSTFVL QGYASYHSPE DTCTKESSTT SDLACPTKTL ASNKGKHQRT PSALSMVSVP 

       670        680        690        700        710        720 
KFLKSSSVPK EQKKAKEEAN INKKSICEKR VEWTFKIFSA SLEPTPEESK NFKKWVQDIK 

       730        740        750        760        770        780 
ALTSFNSTQE RSNFIEEKIL KSRNHNNGKS SQRSKNSTYI TPVDSFVNLS EKVTPSSSVT 

       790        800        810        820        830        840 
TLNTRKRANR PRYIDIPKSA NMNAGAMNSV YRSKVNTPAI DENGNLAIVG ETKNSAPQNG 

       850        860        870        880        890        900 
MSYTIRTPCK SPYSPYTGEG MLYNRSADNL MASSSRKASA PGEVPQIAVS NHGDEAIIPA 

       910        920        930        940        950        960 
SAYSDSSHKS SRASSVASIH NQRVDFYPSP LMNLPGVSPS CLALDGNANG YFGIPLNCNS 

       970        980        990       1000       1010       1020 
EARRGSDLSP FEMESPLFEE NRTQNCSGSR KSSACHIPHQ CGPRKEGNDS RLIYGNEKGA 

      1030       1040       1050       1060       1070       1080 
SQSRLTLKEP LTSKGVEAPY SSLKKTYSAE NVPLTSTVSN DKSLHSRKEG STNTVPATSA 


SSK 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. expand/collapse author list , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"The budding index of Saccharomyces cerevisiae deletion strains identifies genes important for cell cycle progression."
Zettel M.F., Garza L.R., Cass A.M., Myhre R.A., Haizlip L.A., Osadebe S.N., Sudimack D.W., Pathak R., Stone T.L., Polymenis M.
FEMS Microbiol. Lett. 223:253-258(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[4]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"Genome-wide analysis of membrane targeting by S.cerevisiae pleckstrin homology domains."
Yu J.W., Mendrola J.M., Audhya A., Singh S., Keleti D., DeWald D.B., Murray D., Emr S.D., Lemmon M.A.
Mol. Cell 13:677-688(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[7]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-481, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[8]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-481; SER-652; THR-817; SER-866; SER-918 AND SER-1059, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"Identification of positive regulators of the yeast fps1 glycerol channel."
Beese S.E., Negishi T., Levin D.E.
PLoS Genet. 5:E1000738-E1000738(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[10]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-136; SER-249; SER-252; SER-481; SER-537; SER-652; SER-765; SER-813; THR-817; THR-857; SER-866; SER-879; SER-966; SER-969; SER-975; SER-1059; SER-1081 AND SER-1082, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U32445 Genomic DNA. Translation: AAB68085.1.
BK006949 Genomic DNA. Translation: DAA11530.1.
PIRS59780.
RefSeqNP_015440.1. NM_001184212.1.

3D structure databases

ProteinModelPortalQ06108.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36282. 56 interactions.
DIPDIP-1970N.
IntActQ06108. 32 interactions.
MINTMINT-410152.
STRING4932.YPR115W.

Proteomic databases

PaxDbQ06108.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYPR115W; YPR115W; YPR115W.
GeneID856231.
KEGGsce:YPR115W.

Organism-specific databases

CYGDYPR115w.
SGDS000006319. RGC1.

Phylogenomic databases

eggNOGNOG41793.
GeneTreeENSGT00390000002928.
OrthoDBEOG7FZ06S.

Enzyme and pathway databases

BioCycYEAST:G3O-34254-MONOMER.

Gene expression databases

GenevestigatorQ06108.

Family and domain databases

Gene3D2.30.29.30. 2 hits.
InterProIPR011993. PH_like_dom.
IPR001849. Pleckstrin_homology.
[Graphical view]
PfamPF00169. PH. 1 hit.
[Graphical view]
SMARTSM00233. PH. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio981479.

Entry information

Entry nameRGC1_YEAST
AccessionPrimary (citable) accession number: Q06108
Secondary accession number(s): D6W4B4
Entry history
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: November 1, 1996
Last modified: March 19, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XVI

Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families