Reviewed, UniProtKB/Swiss-Prot Q06098 (ISR1_YEAST)

Names and origin

Protein names

Recommended name:
    Serine/threonine-protein kinase ISR1
    EC=2.7.11.1
Alternative name(s):
    Inhibition of staurosporine resistance protein 1

Gene names

Name:	ISR1
Ordered Locus Names:	YPR106W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	443 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Probable serine/threonine protein kinase which may function redundantly with MPK1-independent branch of the PCK1 pathway that is presumed to be required for the tolerance to high temperatures and stauroporin. Ref.2
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Miscellaneous	Present with 1590 molecules/cell in log phase SD medium. Ref.3
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CHS2	P14180	1	EBI-38581,EBI-4624
GCN1	P33892	1	EBI-38581,EBI-7442
GIR2	Q03768	1	EBI-38581,EBI-7618

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 443	443	Serine/threonine-protein kinase ISR1		PRO_0000086038
Regions
	Domain	135 – 415	281	Protein kinase
	Nucleotide binding	141 – 149	9	ATP By similarity
Sites
	Active site	280	1	Proton acceptor By similarity
	Binding site	169	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q06098-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: FBB31592EEF9349F	FASTA	443	49,736
	10 20 30 40 50 60 MNSTPPTSPV TRVSDGSFPS ISNNSKGFAY RQPQKHKSNF AYSHLVSPVE EPTAKFSEAF 70 80 90 100 110 120 QTDYSSKAPV ATSEAHLKND LDVLFTTPRF YSPENLALMF RLSNTVSSLE FLDEFLMGIL 130 140 150 160 170 180 LAPEMDFLSN PSYSLPSNKL VGQGSYSYVY PISSSASSRC NNDSGVVLKF AKSQHKSKVI 190 200 210 220 230 240 LQEALTLAYL QYMSPSTNES HIIPFYGLTY ITKSHFRRLR SNECVPGLIL PKCEMSLYHF 250 260 270 280 290 300 NTAVSHKLSL ITKRKIWWRL MKQMIDALKS LKTNGIIHGD IKTANILITE MHVLNGGHCK 310 320 330 340 350 360 DFDFYLADFT SAFHINQTPT DLNTTVEYCA PELIDSSSDH VPTFESDLYA VGLCLLSFIS 370 380 390 400 410 420 QHEPYNELQA LVSHGSSPGI GSSSIQQSQW LINALLKKDP INLNMLRNDL FQDWKSELAL 430 440 LSRILVDRLP LENLITILDS NYI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed: 9169875] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Functional interaction of Isr1, a predicted protein kinase, with the Pkc1 pathway in Saccharomyces cerevisiae." Miyahara K., Hirata D., Miyakawa T. Biosci. Biotechnol. Biochem. 62:1376-1380(1998) [PubMed: 9720220] [Abstract] Cited for: FUNCTION.
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U32445 Genomic DNA. Translation: AAB68076.1.
PIR	S59771.
RefSeq	NP_015431.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-3818N.
IntAct	Q06098. 4 interactions.
STRING	Q06098.
Genome annotation databases
Ensembl	YPR106W; YPR106W; YPR106W; Saccharomyces cerevisiae. [Genome view]
GeneID	856221.
KEGG	sce:YPR106W.
NMPDR	fig|4932.3.peg.6567.
Organism-specific databases
CYGD	YPR106w.
SGD	S000006310. ISR1.
Phylogenomic databases
OrthoDB	EOG9W0ZW9.
Enzyme and pathway databases
BRENDA	2.7.11.1. 250.
Gene expression databases
ArrayExpress	Q06098.
Genevestigator	Q06098.
GermOnline	YPR106W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_prot_kinase-like_dom. IPR008271. Ser/Thr_prot_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	981450.

Entry information

Entry name

ISR1_YEAST

Accession

Primary (citable) accession number: Q06098

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2005
Last sequence update:	November 1, 1996
Last modified:	December 15, 2009
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)