Reviewed, UniProtKB/Swiss-Prot Q06067 (ATOS_ECOLI)

Names and origin

Protein names

Recommended name:
    Signal transduction histidine-protein kinase atoS
    EC=2.7.13.3

Gene names

Name:	atoS
Ordered Locus Names:	b2219, JW2213

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	608 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Member of the two-component regulatory system atoS/atoC; may activate atoC by phosphorylation.
Catalytic activity	ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.
Subcellular location	Cell inner membrane; Multi-pass membrane protein Ref.5.
Sequence similarities	Contains 1 HAMP domain. Contains 1 histidine kinase domain. Contains 1 PAC (PAS-associated C-terminal) domain. Contains 1 PAS (PER-ARNT-SIM) domain.

Ontologies

Keywords
Biological process	Two-component regulatory system
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	peptidyl-histidine phosphorylation Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro two-component signal transduction system (phosphorelay) Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW two-component sensor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 608	608	Signal transduction histidine-protein kinase atoS		PRO_0000074695
Regions
	Transmembrane	16 – 36	21	Potential
	Transmembrane	190 – 210	21	Potential
	Transmembrane	584 – 604	21	Potential
	Domain	212 – 259	48	HAMP
	Domain	260 – 325	66	PAS
	Domain	326 – 382	57	PAC
	Domain	395 – 602	208	Histidine kinase
Amino acid modifications
	Modified residue	398	1	Phosphohistidine; by autocatalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q06067-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 4669888F98985C9C	FASTA	608	67,790
	10 20 30 40 50 60 MHYMKWIYPR RLRNQMILMA ILMVIVPTLT IGYIVETEGR SAVLSEKEKK LSAVVNLLNQ 70 80 90 100 110 120 ALGDRYDLYI DLPREERIRA LNAELAPITE NITHAFPGIG AGYYNKMLDA IITYAPSALY 130 140 150 160 170 180 QNNVGVTIAA DHPGREVMRT NTPLVYSGRQ VRGDILNSML PIERNGEILG YIWANELTED 190 200 210 220 230 240 IRRQAWKMDV RIIIVLTAGL LISLLLIVLF SRRLSANIDI ITDGLSTLAQ NIPTRLPQLP 250 260 270 280 290 300 GEMGQISQSV NNLAQALRET RTLNDLIIEN AADGVIAIDR QGDVTTMNPA AEVITGYQRH 310 320 330 340 350 360 ELVGQPYSML FDNTQFYSPV LDTLEHGTEH VALEISFPGR DRTIELSVTT SRIHNTHGEM 370 380 390 400 410 420 IGALVIFSDL TARKETQRRM AQAERLATLG ELMAGVAHEV RNPLTAIRGY VQILRQQTSD 430 440 450 460 470 480 PIHQEYLSVV LKEIDSINKV IQQLLEFSRP RHSQWQQVSL NALVEETLVL VQTAGVQARV 490 500 510 520 530 540 DFISELDNEL SPINADRELL KQVLLNILIN AVQAISARGK IRIQTWQYSD SQQAISIEDN 550 560 570 580 590 600 GCGIDLSLQK KIFDPFFTTK ASGTGLGLAL SQRIINAHQG DIRVASLPGY GATFTLILPI NPQGNQTV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification, cloning, and nucleotide sequencing of the ornithine decarboxylase antizyme gene of Escherichia coli." Canellakis E.S., Paterakis A.A., Huang S.-C., Panagiotidis C.A., Kyriakidis D.A. Proc. Natl. Acad. Sci. U.S.A. 90:7129-7133(1993) [PubMed: 8346225] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S. , Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y., Horiuchi T. DNA Res. 3:379-392(1996) [PubMed: 9097040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed: 15919996] [Abstract] Cited for: SUBCELLULAR LOCATION. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L13078 Genomic DNA. Translation: AAA23449.1. U00096 Genomic DNA. Translation: AAC75279.1. AP009048 Genomic DNA. Translation: BAA16015.1.
PIR	A64992.
RefSeq	AP_002818.1. NP_416723.1.
3D structure databases
SMR	Q06067. Positions 254-371, 376-602.
ModBase	Search...
Protein-protein interaction databases
STRING	Q06067.
Genome annotation databases
GeneID	949011.
GenomeReviews	Gene locus JW2213 in contig AP009048_GR. Gene locus b2219 in contig U00096_GR.
KEGG	ecj:JW2213. eco:b2219.
Organism-specific databases
EchoBASE	EB1618.
EcoGene	EG11667. atoS.
CMR	Search...
Phylogenomic databases
eggNOG	COG0642.
HOGENOM	HBG364610.
OMA	KDSIEQY.
Enzyme and pathway databases
BioCyc	EcoCyc:ATOS-MONOMER. ECOL168927:B2219-MONOMER.
BRENDA	2.7.13.3. 246.
Gene expression databases
Genevestigator	Q06067.
Family and domain databases
InterPro	IPR003594. ATPase-like_ATP-bd. IPR003660. HAMP_linker_domain. IPR000014. PAS. IPR000700. PAS-assoc_C. IPR013767. PAS_fold. IPR004358. Sig_transdc_His_kin-like_C. IPR003661. Sig_transdc_His_kin_sub1_dim/P. IPR005467. Sig_transdc_His_kinase_core. IPR009082. Sig_transdc_His_kinase_dimeric. [Graphical view]
Gene3D	G3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit.
Pfam	PF00672. HAMP. 1 hit. PF02518. HATPase_c. 1 hit. PF00512. HisKA. 1 hit. PF00989. PAS. 1 hit. [Graphical view]
PRINTS	PR00344. BCTRLSENSOR.
SMART	SM00304. HAMP. 1 hit. SM00387. HATPase_c. 1 hit. SM00388. HisKA. 1 hit. SM00091. PAS. 1 hit. [Graphical view]
TIGRFAMs	TIGR00229. sensory_box. 1 hit.
PROSITE	PS50885. HAMP. 1 hit. PS50109. HIS_KIN. 1 hit. PS50113. PAC. 1 hit. PS50112. PAS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ATOS_ECOLI

Accession

Primary (citable) accession number: Q06067

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	February 9, 2010
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)