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Protein

Signal transduction histidine-protein kinase AtoS

Gene

atoS

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Member of the two-component regulatory system AtoS/AtoC. In the presence of acetoacetate, AtoS/AtoC stimulates the expression of the atoDAEB operon, leading to short chain fatty acid catabolism and activation of the poly-(R)-3-hydroxybutyrate (cPHB) biosynthetic pathway. Also induces the operon in response to spermidine (PubMed:16153782, PubMed:16564134, PubMed:17475408). Involved in the regulation of motility and chemotaxis, via transcriptional induction of the flagellar regulon (PubMed:22083893). AtoS is a membrane-associated kinase that phosphorylates and activates AtoC in response to environmental signals (PubMed:16153782, PubMed:18534200).5 Publications

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.2 Publications

GO - Molecular functioni

  • ATP binding Source: EcoCyc
  • phosphorelay sensor kinase activity Source: EcoCyc

GO - Biological processi

  • phosphorelay signal transduction system Source: EcoCyc
  • protein autophosphorylation Source: EcoCyc
  • regulation of transcription, DNA-templated Source: InterPro

Keywordsi

Molecular functionKinase, Transferase
Biological processTwo-component regulatory system
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:ATOS-MONOMER.
BRENDAi2.7.13.3. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
Signal transduction histidine-protein kinase AtoSCurated (EC:2.7.13.32 Publications)
Gene namesi
Name:atoS
Ordered Locus Names:b2219, JW2213
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11667. atoS.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 15CytoplasmicCuratedAdd BLAST15
Transmembranei16 – 36HelicalSequence analysisAdd BLAST21
Topological domaini37 – 189PeriplasmicCuratedAdd BLAST153
Transmembranei190 – 210HelicalSequence analysisAdd BLAST21
Topological domaini211 – 608CytoplasmicCuratedAdd BLAST398

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • intracellular Source: GOC
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Deletion of the atoSC locus renders cells not motile or responsive against any chemoattractant or repellent independently of the atoSC inducer's presence.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi398H → L: Lack of phosphorylation. Cannot respond to acetoacetate induction. 1 Publication1
Mutagenesisi565G → A: Cannot bind ATP and is unable to autophosphorylate. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000746951 – 608Signal transduction histidine-protein kinase AtoSAdd BLAST608

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei398Phosphohistidine; by autocatalysisPROSITE-ProRule annotation1 Publication1

Post-translational modificationi

Autophosphorylated (PubMed:16153782, PubMed:18534200). Each AtoS molecule may phosphorylate its partner within the dimer rather than phosphorylating itself (PubMed:18534200).2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ06067.
PRIDEiQ06067.

PTM databases

iPTMnetiQ06067.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi4262011. 13 interactors.
IntActiQ06067. 2 interactors.
STRINGi316385.ECDH10B_2377.

Structurei

3D structure databases

ProteinModelPortaliQ06067.
SMRiQ06067.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini212 – 262HAMPPROSITE-ProRule annotationAdd BLAST51
Domaini260 – 305PASPROSITE-ProRule annotationAdd BLAST46
Domaini326 – 382PACPROSITE-ProRule annotationAdd BLAST57
Domaini395 – 602Histidine kinasePROSITE-ProRule annotationAdd BLAST208

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105BZU. Bacteria.
COG0642. LUCA.
COG2202. LUCA.
HOGENOMiHOG000122301.
InParanoidiQ06067.
KOiK07710.
PhylomeDBiQ06067.

Family and domain databases

CDDicd00075. HATPase_c. 1 hit.
cd00082. HisKA. 1 hit.
cd00130. PAS. 1 hit.
Gene3Di3.30.565.10. 1 hit.
InterProiView protein in InterPro
IPR003660. HAMP_dom.
IPR003594. HATPase_C.
IPR036890. HATPase_C_sf.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR036097. HisK_dim/P_sf.
IPR000014. PAS.
IPR000700. PAS-assoc_C.
IPR035965. PAS-like_dom_sf.
IPR013767. PAS_fold.
IPR004358. Sig_transdc_His_kin-like_C.
PfamiView protein in Pfam
PF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF00989. PAS. 1 hit.
PRINTSiPR00344. BCTRLSENSOR.
SMARTiView protein in SMART
SM00304. HAMP. 1 hit.
SM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
SM00091. PAS. 1 hit.
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF55785. SSF55785. 1 hit.
SSF55874. SSF55874. 1 hit.
TIGRFAMsiTIGR00229. sensory_box. 1 hit.
PROSITEiView protein in PROSITE
PS50885. HAMP. 1 hit.
PS50109. HIS_KIN. 1 hit.
PS50113. PAC. 1 hit.
PS50112. PAS. 1 hit.

Sequencei

Sequence statusi: Complete.

Q06067-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHYMKWIYPR RLRNQMILMA ILMVIVPTLT IGYIVETEGR SAVLSEKEKK
60 70 80 90 100
LSAVVNLLNQ ALGDRYDLYI DLPREERIRA LNAELAPITE NITHAFPGIG
110 120 130 140 150
AGYYNKMLDA IITYAPSALY QNNVGVTIAA DHPGREVMRT NTPLVYSGRQ
160 170 180 190 200
VRGDILNSML PIERNGEILG YIWANELTED IRRQAWKMDV RIIIVLTAGL
210 220 230 240 250
LISLLLIVLF SRRLSANIDI ITDGLSTLAQ NIPTRLPQLP GEMGQISQSV
260 270 280 290 300
NNLAQALRET RTLNDLIIEN AADGVIAIDR QGDVTTMNPA AEVITGYQRH
310 320 330 340 350
ELVGQPYSML FDNTQFYSPV LDTLEHGTEH VALEISFPGR DRTIELSVTT
360 370 380 390 400
SRIHNTHGEM IGALVIFSDL TARKETQRRM AQAERLATLG ELMAGVAHEV
410 420 430 440 450
RNPLTAIRGY VQILRQQTSD PIHQEYLSVV LKEIDSINKV IQQLLEFSRP
460 470 480 490 500
RHSQWQQVSL NALVEETLVL VQTAGVQARV DFISELDNEL SPINADRELL
510 520 530 540 550
KQVLLNILIN AVQAISARGK IRIQTWQYSD SQQAISIEDN GCGIDLSLQK
560 570 580 590 600
KIFDPFFTTK ASGTGLGLAL SQRIINAHQG DIRVASLPGY GATFTLILPI

NPQGNQTV
Length:608
Mass (Da):67,790
Last modified:February 1, 1995 - v1
Checksum:i4669888F98985C9C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L13078 Genomic DNA. Translation: AAA23449.1.
U00096 Genomic DNA. Translation: AAC75279.1.
AP009048 Genomic DNA. Translation: BAA16015.1.
PIRiA64992.
RefSeqiNP_416723.1. NC_000913.3.
WP_000559125.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75279; AAC75279; b2219.
BAA16015; BAA16015; BAA16015.
GeneIDi949011.
KEGGiecj:JW2213.
eco:b2219.
PATRICifig|1411691.4.peg.16.

Similar proteinsi

Entry informationi

Entry nameiATOS_ECOLI
AccessioniPrimary (citable) accession number: Q06067
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: October 25, 2017
This is version 153 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene