Signal transduction histidine-protein kinase AtoS

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Q06067 (ATOS_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 108. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Signal transduction histidine-protein kinase AtoS
EC=2.7.13.3

Gene names

Name:	atoS
Ordered Locus Names:	b2219, JW2213

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	608 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Member of the two-component regulatory system AtoS/AtoC; may activate AtoC by phosphorylation.
Catalytic activity	ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.
Subcellular location	Cell inner membrane; Multi-pass membrane protein Ref.5.
Sequence similarities	Contains 1 HAMP domain. Contains 1 histidine kinase domain. Contains 1 PAC (PAS-associated C-terminal) domain. Contains 1 PAS (PER-ARNT-SIM) domain.

Ontologies

Keywords
Biological process	Two-component regulatory system
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	peptidyl-histidine phosphorylation Inferred from electronic annotation. Source: InterPro protein autophosphorylation Inferred from direct assay. Source: EcoCyc regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane enriched fraction Inferred from direct assay. Source: EcoCyc
Molecular function	ATP binding Inferred from mutant phenotype. Source: EcoCyc two-component sensor activity Inferred from direct assay. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 608

608

Signal transduction histidine-protein kinase AtoS

PRO_0000074695

Regions

Transmembrane

16 – 36

Helical; Potential

Transmembrane

190 – 210

Helical; Potential

Transmembrane

584 – 604

Helical; Potential

Domain

212 – 259

HAMP

Domain

260 – 325

PAS

Domain

326 – 382

PAC

Domain

395 – 602

208

Histidine kinase

Amino acid modifications

Modified residue

398

Phosphohistidine; by autocatalysis By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q06067 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 4669888F98985C9C
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FASTA

608

67,790

        10         20         30         40         50         60 
MHYMKWIYPR RLRNQMILMA ILMVIVPTLT IGYIVETEGR SAVLSEKEKK LSAVVNLLNQ 

        70         80         90        100        110        120 
ALGDRYDLYI DLPREERIRA LNAELAPITE NITHAFPGIG AGYYNKMLDA IITYAPSALY 

       130        140        150        160        170        180 
QNNVGVTIAA DHPGREVMRT NTPLVYSGRQ VRGDILNSML PIERNGEILG YIWANELTED 

       190        200        210        220        230        240 
IRRQAWKMDV RIIIVLTAGL LISLLLIVLF SRRLSANIDI ITDGLSTLAQ NIPTRLPQLP 

       250        260        270        280        290        300 
GEMGQISQSV NNLAQALRET RTLNDLIIEN AADGVIAIDR QGDVTTMNPA AEVITGYQRH 

       310        320        330        340        350        360 
ELVGQPYSML FDNTQFYSPV LDTLEHGTEH VALEISFPGR DRTIELSVTT SRIHNTHGEM 

       370        380        390        400        410        420 
IGALVIFSDL TARKETQRRM AQAERLATLG ELMAGVAHEV RNPLTAIRGY VQILRQQTSD 

       430        440        450        460        470        480 
PIHQEYLSVV LKEIDSINKV IQQLLEFSRP RHSQWQQVSL NALVEETLVL VQTAGVQARV 

       490        500        510        520        530        540 
DFISELDNEL SPINADRELL KQVLLNILIN AVQAISARGK IRIQTWQYSD SQQAISIEDN 

       550        560        570        580        590        600 
GCGIDLSLQK KIFDPFFTTK ASGTGLGLAL SQRIINAHQG DIRVASLPGY GATFTLILPI 


NPQGNQTV

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification, cloning, and nucleotide sequencing of the ornithine decarboxylase antizyme gene of Escherichia coli." Canellakis E.S., Paterakis A.A., Huang S.-C., Panagiotidis C.A., Kyriakidis D.A. Proc. Natl. Acad. Sci. U.S.A. 90:7129-7133(1993) [PubMed: 8346225] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S. Horiuchi T. DNA Res. 3:379-392(1996) [PubMed: 9097040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed: 15919996] [Abstract] Cited for: SUBCELLULAR LOCATION. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L13078 Genomic DNA. Translation: AAA23449.1. U00096 Genomic DNA. Translation: AAC75279.1. AP009048 Genomic DNA. Translation: BAA16015.1.
PIR	A64992.
RefSeq	NP_416723.1. NC_000913.2.
3D structure databases
ProteinModelPortal	Q06067.
SMR	Q06067. Positions 263-604.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q06067. 1 interaction.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000004380; EBESCP00000004380; EBESCG00000003571. EBESCT00000017103; EBESCP00000016394; EBESCG00000016162.
GeneID	949011.
GenomeReviews	Gene locus JW2213 in contig AP009048_GR. Gene locus b2219 in contig U00096_GR.
KEGG	ecj:JW2213. eco:b2219.
PATRIC	32119797. VBIEscCol129921_2308.
Organism-specific databases
EchoBASE	EB1618.
EcoGene	EG11667. atoS.
Phylogenomic databases
eggNOG	COG0642.
GeneTree	EBGT00050000008663.
HOGENOM	HBG364610.
OMA	NPIEQYP.
PhylomeDB	Q06067.
ProtClustDB	PRK11360.
Enzyme and pathway databases
BioCyc	EcoCyc:ATOS-MONOMER.
BRENDA	2.7.13.3. 2026.
Gene expression databases
Genevestigator	Q06067.
Family and domain databases
InterPro	IPR003594. ATPase-like_ATP-bd. IPR003660. HAMP_linker_domain. IPR000014. PAS. IPR000700. PAS-assoc_C. IPR013767. PAS_fold. IPR004358. Sig_transdc_His_kin-like_C. IPR003661. Sig_transdc_His_kin_sub1_dim/P. IPR005467. Sig_transdc_His_kinase_core. IPR009082. Sig_transdc_His_kinase_dimeric. [Graphical view]
Gene3D	G3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit.
KO	K07710.
Pfam	PF00672. HAMP. 1 hit. PF02518. HATPase_c. 1 hit. PF00512. HisKA. 1 hit. PF00989. PAS. 1 hit. [Graphical view]
PRINTS	PR00344. BCTRLSENSOR.
SMART	SM00304. HAMP. 1 hit. SM00387. HATPase_c. 1 hit. SM00388. HisKA. 1 hit. SM00091. PAS. 1 hit. [Graphical view]
SUPFAM	SSF55874. ATP_bd_ATPase. 1 hit. SSF47384. His_kin_homodim. 1 hit.
TIGRFAMs	TIGR00229. Sensory_box. 1 hit.
PROSITE	PS50885. HAMP. 1 hit. PS50109. HIS_KIN. 1 hit. PS50113. PAC. 1 hit. PS50112. PAS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ATOS_ECOLI

Accession

Primary (citable) accession number: Q06067

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	January 25, 2012
This is version 108 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents