Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Regulatory protein AtoC

Gene

atoC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Member of the two-component regulatory system AtoS/AtoC. In the presence of acetoacetate, AtoS/AtoC stimulates the expression of the atoDAEB operon, leading to short chain fatty acid catabolism and activation of the poly-(R)-3-hydroxybutyrate (cPHB) biosynthetic pathway. Also induces the operon in response to spermidine (PubMed:2883171, PubMed:16153782, PubMed:16564134, PubMed:17475408). Involved in the regulation of motility and chemotaxis, via transcriptional induction of the flagellar regulon (PubMed:22083893). AtoC acts by binding directly to the promoter region of the target genes (PubMed:17616594). In addition to its role as a transcriptional regulator, functions as a post-translational regulator that inhibits polyamine biosynthesis via regulation of ornithine decarboxylase (ODC) (PubMed:8346225).7 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi173 – 180ATP8
Nucleotide bindingi236 – 245ATP10
DNA bindingi433 – 452H-T-H motifBy similarityAdd BLAST20

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation, Two-component regulatory system
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:ATOC-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Regulatory protein AtoCCurated
Alternative name(s):
Acetoacetate metabolism regulatory proteinCurated
DNA-binding transcriptional regulator AtoCCurated
Ornithine decarboxylase antizyme1 Publication
Gene namesi
Name:atoC
Synonyms:az1 Publication
Ordered Locus Names:b2220, JW2214
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11668. atoC.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Deletion of the atoSC locus renders cells not motile or responsive against any chemoattractant or repellent independently of the atoSC inducer's presence.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi55D → G: Decreases phosphorylation. Significant decrease in ability to respond to acetoacetate induction. 40% decrease in cPHB accumulation. Cannot respond to acetoacetate induction; when associated with L-73. 2 Publications1
Mutagenesisi73H → L: Decreases phosphorylation. Decrease in ability to respond to acetoacetate induction. 70% decrease in cPHB accumulation. Cannot respond to acetoacetate induction; when associated with G-55. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000810201 – 461Regulatory protein AtoCAdd BLAST461

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei554-aspartylphosphate1 Publication1
Modified residuei73Phosphohistidine1 Publication1

Post-translational modificationi

Phosphorylated by AtoS (PubMed:16153782). Contains two phosphorylation sites, which are both involved in the transduction of the acetoacetate signal (PubMed:16153782). Asp-55 is probably the primary phosphorylation site, but either both residues can be phosphorylated independently by AtoS or the phosphate group can be transferred between them (PubMed:16153782).1 Publication
The N-terminus is blocked.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ06065.
PRIDEiQ06065.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-9190N.
IntActiQ06065. 10 interactors.
STRINGi316385.ECDH10B_2378.

Structurei

3D structure databases

ProteinModelPortaliQ06065.
SMRiQ06065.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini6 – 120Response regulatoryAdd BLAST115
Domaini145 – 374Sigma-54 factor interactionAdd BLAST230

Phylogenomic databases

eggNOGiENOG4105C1W. Bacteria.
COG2204. LUCA.
HOGENOMiHOG000058489.
InParanoidiQ06065.
KOiK07714.
PhylomeDBiQ06065.

Family and domain databases

CDDicd00156. REC. 1 hit.
InterProiView protein in InterPro
IPR003593. AAA+_ATPase.
IPR011006. CheY-like_superfamily.
IPR009057. Homeobox-like_sf.
IPR002197. HTH_Fis.
IPR027417. P-loop_NTPase.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR002078. Sigma_54_int.
IPR025662. Sigma_54_int_dom_ATP-bd_1.
IPR025943. Sigma_54_int_dom_ATP-bd_2.
IPR025944. Sigma_54_int_dom_CS.
PfamiView protein in Pfam
PF02954. HTH_8. 1 hit.
PF00072. Response_reg. 1 hit.
PF00158. Sigma54_activat. 1 hit.
PRINTSiPR01590. HTHFIS.
SMARTiView protein in SMART
SM00382. AAA. 1 hit.
SM00448. REC. 1 hit.
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF52172. SSF52172. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiView protein in PROSITE
PS50110. RESPONSE_REGULATORY. 1 hit.
PS00675. SIGMA54_INTERACT_1. 1 hit.
PS00676. SIGMA54_INTERACT_2. 1 hit.
PS00688. SIGMA54_INTERACT_3. 1 hit.
PS50045. SIGMA54_INTERACT_4. 1 hit.

Sequencei

Sequence statusi: Complete.

Q06065-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAINRILIV DDEDNVRRML STAFALQGFE THCANNGRTA LHLFADIHPD
60 70 80 90 100
VVLMDIRMPE MDGIKALKEM RSHETRTPVI LMTAYAEVET AVEALRCGAF
110 120 130 140 150
DYVIKPFDLD ELNLIVQRAL QLQSMKKEIR HLHQALSTSW QWGHILTNSP
160 170 180 190 200
AMMDICKDTA KIALSQASVL ISGESGTGKE LIARAIHYNS RRAKGPFIKV
210 220 230 240 250
NCAALPESLL ESELFGHEKG AFTGAQTLRQ GLFERANEGT LLLDEIGEMP
260 270 280 290 300
LVLQAKLLRI LQEREFERIG GHQTIKVDIR IIAATNRDLQ AMVKEGTFRE
310 320 330 340 350
DLFYRLNVIH LILPPLRDRR EDISLLANHF LQKFSSENQR DIIDIDPMAM
360 370 380 390 400
SLLTAWSWPG NIRELSNVIE RAVVMNSGPI IFSEDLPPQI RQPVCNAGEV
410 420 430 440 450
KTAPVGERNL KEEIKRVEKR IIMEVLEQQE GNRTRTALML GISRRALMYK
460
LQEYGIDPAD V
Length:461
Mass (Da):52,176
Last modified:November 1, 1995 - v2
Checksum:i478D5795790E339A
GO

Sequence cautioni

The sequence AAA23450 differs from that shown. Reason: Frameshift at position 92.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti99A → R in AAA23450 (PubMed:8346225).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U17902 Genomic DNA. Translation: AAA60332.1.
U00096 Genomic DNA. Translation: AAC75280.1.
AP009048 Genomic DNA. Translation: BAA16016.1.
L13078 Genomic DNA. Translation: AAA23450.1. Frameshift.
PIRiB64992.
RefSeqiNP_416724.1. NC_000913.3.
WP_000125282.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75280; AAC75280; b2220.
BAA16016; BAA16016; BAA16016.
GeneIDi947444.
KEGGiecj:JW2214.
eco:b2220.
PATRICifig|1411691.4.peg.15.

Similar proteinsi

Entry informationi

Entry nameiATOC_ECOLI
AccessioniPrimary (citable) accession number: Q06065
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: November 1, 1995
Last modified: November 22, 2017
This is version 161 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene