Reviewed, UniProtKB/Swiss-Prot Q06060 (MAPK_PEA)

Names and origin

Protein names	Recommended name:     Mitogen-activated protein kinase homolog D5     EC=2.7.11.24
Organism	Pisum sativum (Garden pea)
Taxonomic identifier	3888 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Fabales › Fabaceae › Papilionoideae › Fabeae › Pisum

Protein attributes

Sequence length	394 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Cofactor	Magnesium By similarity.
Enzyme regulation	Activated by tyrosine and threonine phosphorylation By similarity.
Tissue specificity	Leaves, roots, root apices, and dormant and growing axillary buds.
Domain	The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
Post-translational modification	Dually phosphorylated on Thr-220 and Tyr-222, which activates the enzyme By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. Contains 1 protein kinase domain.
Caution	It is uncertain whether Met-1, Met-14 or Met-30 is the initiator.

Ontologies

Keywords
Biological process	Cell cycle
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW MAP kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 394	394	Mitogen-activated protein kinase homolog D5		PRO_0000186319
Regions
	Domain	62 – 347	286	Protein kinase
	Nucleotide binding	68 – 76	9	ATP By similarity
	Motif	220 – 222	3	TXY
Sites
	Active site	188	1	Proton acceptor By similarity
	Binding site	91	1	ATP By similarity
Amino acid modifications
	Modified residue	220	1	Phosphothreonine By similarity
	Modified residue	222	1	Phosphotyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q06060-1 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: E7B4758EBD8F24A9	FASTA	394	45,145
	10 20 30 40 50 60 MEGGGGAPAA DAVMEDAAPQ QQEPQQQAAM GIENIPATLS HGGRFIQYNI FGNIFEVTAK 70 80 90 100 110 120 YRPPIMPIGK GAYGIVCSAH NSETNEHVAV KKIANAFDNK IDAKRTLREI KLVRHMDHEN 130 140 150 160 170 180 VVAIRDIVPP PQREVFNDVY IAYELMDTDL HQIIRSNQAL SEEHCQYFLY QILRGLKYIH 190 200 210 220 230 240 SANVLHRDLK PSNLLLNANC DLKICDFGLA RVTSETDFMT EYVVTRWYRA PELLLNSSDY 250 260 270 280 290 300 TAAIDVWSVG CIFMELMDRK PLFPGRDHVH QLRLLMELIG TPSEADLGFL NENAKRYIRQ 310 320 330 340 350 360 LPLYRRQSFQ EKFPHVHPEA IDLVEKMLTF DPRQRITVEN ALAHPYLTSL HDISDEPVCT 370 380 390 TPFSFDFEQH ALTEEQMKEL IYREALAFNP EYQQ

References

[1]	"Molecular cloning and expression of a MAP kinase homologue from pea." Stafstrom J.P., Altschuler M., Anderson D.H. Plant Mol. Biol. 22:83-90(1993) [PubMed: 8388749] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Alaska. Tissue: Seedling.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X70703 mRNA. Translation: CAA50036.1.
PIR	S33635.
3D structure databases
HSSP	HSSP built from PDB template 1KV1 based on UniProtKB Q16539.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.7.11.24. 287.
Family and domain databases
InterPro	IPR008351. JNK_MAPK. IPR003527. MAP_kinase_CS. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
PRINTS	PR01772. JNKMAPKINASE.
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS01351. MAPK. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MAPK_PEA

Accession

Primary (citable) accession number: Q06060

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	June 16, 2009
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)