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Q06060 (MPK_PEA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitogen-activated protein kinase homolog D5

EC=2.7.11.24
OrganismPisum sativum (Garden pea)
Taxonomic identifier3888 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeFabeaePisum

Protein attributes

Sequence length394 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Cofactor

Magnesium By similarity.

Enzyme regulation

Activated by tyrosine and threonine phosphorylation By similarity.

Tissue specificity

Leaves, roots, root apices, and dormant and growing axillary buds.

Domain

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Post-translational modification

Dually phosphorylated on Thr-220 and Tyr-222, which activates the enzyme By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

Contains 1 protein kinase domain.

Caution

It is uncertain whether Met-1, Met-14 or Met-30 is the initiator.

Ontologies

Keywords
   Biological processCell cycle
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Serine/threonine-protein kinase
Transferase
   PTMPhosphoprotein
Gene Ontology (GO)
   Biological_processcell cycle

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

MAP kinase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 394394Mitogen-activated protein kinase homolog D5
PRO_0000186319

Regions

Domain62 – 347286Protein kinase
Nucleotide binding68 – 769ATP By similarity
Motif220 – 2223TXY

Sites

Active site1881Proton acceptor By similarity
Binding site911ATP By similarity

Amino acid modifications

Modified residue2201Phosphothreonine By similarity
Modified residue2221Phosphotyrosine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q06060 [UniParc].

Last modified October 1, 1994. Version 1.
Checksum: E7B4758EBD8F24A9

FASTA39445,145
        10         20         30         40         50         60 
MEGGGGAPAA DAVMEDAAPQ QQEPQQQAAM GIENIPATLS HGGRFIQYNI FGNIFEVTAK 

        70         80         90        100        110        120 
YRPPIMPIGK GAYGIVCSAH NSETNEHVAV KKIANAFDNK IDAKRTLREI KLVRHMDHEN 

       130        140        150        160        170        180 
VVAIRDIVPP PQREVFNDVY IAYELMDTDL HQIIRSNQAL SEEHCQYFLY QILRGLKYIH 

       190        200        210        220        230        240 
SANVLHRDLK PSNLLLNANC DLKICDFGLA RVTSETDFMT EYVVTRWYRA PELLLNSSDY 

       250        260        270        280        290        300 
TAAIDVWSVG CIFMELMDRK PLFPGRDHVH QLRLLMELIG TPSEADLGFL NENAKRYIRQ 

       310        320        330        340        350        360 
LPLYRRQSFQ EKFPHVHPEA IDLVEKMLTF DPRQRITVEN ALAHPYLTSL HDISDEPVCT 

       370        380        390 
TPFSFDFEQH ALTEEQMKEL IYREALAFNP EYQQ 

« Hide

References

[1]"Molecular cloning and expression of a MAP kinase homologue from pea."
Stafstrom J.P., Altschuler M., Anderson D.H.
Plant Mol. Biol. 22:83-90(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Alaska.
Tissue: Seedling.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X70703 mRNA. Translation: CAA50036.1.
PIRS33635.

3D structure databases

ProteinModelPortalQ06060.
SMRQ06060. Positions 51-394.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ06060.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

BRENDA2.7.11.24. 4872.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMPK_PEA
AccessionPrimary (citable) accession number: Q06060
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: April 16, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families