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Protein

tRNA-dihydrouridine(47) synthase [NAD(P)(+)]

Gene

DUS3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs. Specifically modifies U47 in cytoplasmic tRNAs.2 Publications

Catalytic activityi

5,6-dihydrouracil(47) in tRNA + NAD(P)+ = uracil(47) in tRNA + NAD(P)H.1 Publication

Cofactori

FMNBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei354FMNBy similarity1
Active sitei386Proton donorBy similarity1
Binding sitei426FMNBy similarity1
Binding sitei457FMNBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri92 – 122C3H1-type 1PROSITE-ProRule annotationAdd BLAST31
Zinc fingeri134 – 164C3H1-type 2PROSITE-ProRule annotationAdd BLAST31
Nucleotide bindingi299 – 301FMNBy similarity3
Nucleotide bindingi509 – 511FMNBy similarity3
Nucleotide bindingi534 – 535FMNBy similarity2

GO - Molecular functioni

GO - Biological processi

  • tRNA modification Source: SGD

Keywordsi

Molecular functionOxidoreductase
Biological processtRNA processing
LigandFlavoprotein, FMN, Metal-binding, NAD, NADP, Zinc

Enzyme and pathway databases

BioCyciMetaCyc:G3O-32464-MONOMER
YEAST:G3O-32464-MONOMER
BRENDAi1.3.1.89 984

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-dihydrouridine(47) synthase [NAD(P)(+)] (EC:1.3.1.89)
Alternative name(s):
tRNA-dihydrouridine synthase 3
Gene namesi
Name:DUS3
Ordered Locus Names:YLR401C
ORF Names:L8084.19
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR401C
SGDiS000004393 DUS3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001621551 – 668tRNA-dihydrouridine(47) synthase [NAD(P)(+)]Add BLAST668

Proteomic databases

MaxQBiQ06053
PaxDbiQ06053
PRIDEiQ06053

PTM databases

iPTMnetiQ06053

Interactioni

Protein-protein interaction databases

BioGridi31659, 99 interactors
DIPiDIP-4469N
MINTiQ06053
STRINGi4932.YLR401C

Structurei

3D structure databases

ProteinModelPortaliQ06053
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Dus family. Dus3 subfamily.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri92 – 122C3H1-type 1PROSITE-ProRule annotationAdd BLAST31
Zinc fingeri134 – 164C3H1-type 2PROSITE-ProRule annotationAdd BLAST31

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00550000075134
HOGENOMiHOG000240610
InParanoidiQ06053
KOiK05544
OMAiWALMKAH
OrthoDBiEOG092C20WI

Family and domain databases

CDDicd02801 DUS_like_FMN, 1 hit
Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR035587 DUS-like_FMN-bd
IPR001269 tRNA_hU_synthase
IPR018517 tRNA_hU_synthase_CS
IPR000571 Znf_CCCH
PANTHERiPTHR11082 PTHR11082, 1 hit
PfamiView protein in Pfam
PF01207 Dus, 2 hits
PROSITEiView protein in PROSITE
PS01136 UPF0034, 1 hit
PS50103 ZF_C3H1, 2 hits

Sequencei

Sequence statusi: Complete.

Q06053-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEQNAEKRSI VGDDNSTVKR QDTSPSKGIA HIKPEYIVPL KQNENQKVAI
60 70 80 90 100
YDEEMSSDRM TNEFAGGTNK KNKNGRGKKR GQNKNRDNRQ VKEQNVLCPR
110 120 130 140 150
LIHGDISKCS FGDNCRFVHD INLYLSTKKP EVESNIFPSC PVFNSLGFCP
160 170 180 190 200
MGFKCRFLSS HLNKEDNILI SKKEIDPDAQ TIWSVKGEVN HISPERKLDL
210 220 230 240 250
IKRRFPFTKS NEILEIIDSF QQECRDSMKP EEEVESTPQL KKQDPDVEQP
260 270 280 290 300
VAPQVEQRNK ELSEHRMKQR EVYLKYKDTR YFAQEKKPLD LYHKKIVSPL
310 320 330 340 350
TTVGNLPYRR LMRKLGADVT YSEMALAVPL IQGTNSEWAL PKAHTSEFPG
360 370 380 390 400
FGVQVACSKA WQAAKAAEAL ANSVSEISEI NLNSGCPIDL LYRQGSGSAL
410 420 430 440 450
LDNPARMIRC LNAMNYVSKD IPITVKIRTG TKEGHPIAEG LVKRLVNETD
460 470 480 490 500
VAAITLHGRS RQQRYTKSAD WDYVSQVADT LRSAEADFIE TEQGKEGRDS
510 520 530 540 550
KNRIQFVGNG DVNNFEDWYR YLNGNENIDS VMVARGALIK PWIFEEVESQ
560 570 580 590 600
QYLDKTSTER LDILRDYAQF SMEHWGTDEY GISQCRRFFC EFMSFFHRYV
610 620 630 640 650
PMGICERYPV KLNERPPNWC GRDELETLMG STDVNDWIKL SDLFFGKTDE
660
NFVFVPKHKS SSYANRDS
Length:668
Mass (Da):76,596
Last modified:April 29, 2008 - v2
Checksum:i0DA116A41699E9C9
GO

Sequence cautioni

The sequence AAB82358 differs from that shown. Reason: Frameshift at position 601.Curated
The sequence CAA79376 differs from that shown. Reason: Frameshift at positions 603, 639 and 656.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U19729 Genomic DNA Translation: AAB82358.1 Frameshift.
Z18944 Genomic DNA Translation: CAA79376.1 Frameshift.
BK006945 Genomic DNA Translation: DAA09701.1
PIRiS55957
RefSeqiNP_013505.4, NM_001182289.3

Genome annotation databases

EnsemblFungiiYLR401C; YLR401C; YLR401C
GeneIDi851117
KEGGisce:YLR401C

Similar proteinsi

Entry informationi

Entry nameiDUS3_YEAST
AccessioniPrimary (citable) accession number: Q06053
Secondary accession number(s): D6VZ35, P39519
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: April 29, 2008
Last modified: May 23, 2018
This is version 148 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

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