Q06031 (GP63_CRIFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Leishmanolysin homolog
EC=3.4.24.36

Alternative name(s):
Cell surface protease
Major surface glycoprotein
Protein gp63

Gene names

Name:

gp63

Organism

Crithidia fasciculata

Taxonomic identifier

5656 [NCBI]

Taxonomic lineage

Eukaryota › Euglenozoa › Kinetoplastida › Trypanosomatidae › Crithidia

Protein attributes

Sequence length	653 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Plays an integral role during the infection of macrophages in the mammalian host By similarity.
Catalytic activity	Preference for hydrophobic residues at P1 and P1' and basic residues at P2' and P3'. A model nonapeptide is cleaved at -Ala-Tyr-|-Leu-Lys-Lys-.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subcellular location	Cell membrane; Lipid-anchor › GPI-anchor By similarity.
Sequence similarities	Belongs to the peptidase M8 family.

Ontologies

Keywords
Biological process	Cell adhesion
Cellular component	Cell membrane Membrane
Domain	Signal
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
PTM	Disulfide bond GPI-anchor Glycoprotein Lipoprotein Zymogen
Gene Ontology (GO)
Biological process	cell adhesion Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	anchored to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 45	45	By similarity
	Propeptide	46 – 113	68	Activation peptide By similarity		PRO_0000028652
	Chain	114 – 628	515	Leishmanolysin homolog		PRO_0000028653
	Propeptide	629 – 653	25	Removed in mature form Potential		PRO_0000028654
Sites
	Active site	277	1	By similarity
	Metal binding	276	1	Zinc; catalytic Potential
	Metal binding	280	1	Zinc; catalytic Potential
	Metal binding	346	1	Zinc; catalytic By similarity
Amino acid modifications
	Lipidation	628	1	GPI-anchor amidated serine Potential
	Glycosylation	383	1	N-linked (GlcNAc...) Potential
	Glycosylation	409	1	N-linked (GlcNAc...) Potential
	Glycosylation	569	1	N-linked (GlcNAc...) Potential
	Disulfide bond	138 ↔ 155		By similarity
	Disulfide bond	203 ↔ 242		By similarity
	Disulfide bond	326 ↔ 398		By similarity
	Disulfide bond	405 ↔ 468		By similarity
	Disulfide bond	418 ↔ 437		By similarity
	Disulfide bond	427 ↔ 502		By similarity
	Disulfide bond	479 ↔ 524		By similarity
	Disulfide bond	529 ↔ 579		By similarity
	Disulfide bond	549 ↔ 572		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q06031 [UniParc]. Last modified October 5, 2010. Version 2. Checksum: 263A1DA7568034B6	FASTA	653	69,171
	10 20 30 40 50 60 MHAPPTATRR SGPRRTHGIM ARLVRLAAGV LVVTLVIGAL TALSADDAKT HPHKVCIHDE 70 80 90 100 110 120 LQQSLLDSVA QQGLAPQRVS RVGLPYVASA TAAPAAQVGG VDFALAGDSA PDVTRSAEWG 130 140 150 160 170 180 ELRITVSAEE LTDPAYHCAT VGQVISNHID DYVTCTADDI MTAEKLDILM NYLIPEALQM 190 200 210 220 230 240 HKDRLQVQQV QGTWKVARMT SYCGRFKVPE EHFTTGLSNT DFVLYVASVP TSPGVLAWAN 250 260 270 280 290 300 TCQVFSNDQP AVGVINIPAA TITERYDHLM VHAVTHEIAH SLGFSNAFFT NTGIGQFVTG 310 320 330 340 350 360 VRGNPDTVPV INSPTVVAKA REHYGCDDVT YVELEDAGGS GTMGSHWKIR NAQDELMAGI 370 380 390 400 410 420 SGVAYYTSLT LSAFEDLGYY KANYSNAETM KWGKDVGCAF LTGKCVVDNV TQFPSMYCDK 430 440 450 460 470 480 DENVYRCHTA RLNLGSCEVT DYTFDLPDYL QYFTVPSVGG SADYYDYCPY IVRSPIGSCT 490 500 510 520 530 540 QAASSASPFV SAFNTFSMAS RCIDGTFTPK STGGATVTAH LGMCTNVACN TADKTYSIQV 550 560 570 580 590 600 YGNGAYIPCT PGATISLDTV SDAFEAGGNI TCPPYLEVCQ SNVKGAMDYE SMTNSGSGSS 610 620 630 640 650 RPAPVEPSGS GSGSSAATTA PSPTRDGSAA ADRIAPRTAA VALLALAVAA ACV

References

[1]	"Crithidia fasciculata contains a transcribed leishmanial surface proteinase (gp63) gene homologue." Inverso J.A., Medina-Acosta E., O'Connor J., Russell D.G., Cross G.A.M. Mol. Biochem. Parasitol. 57:47-54(1993) [PubMed: 8426615] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 11745.
[2]	Inverso J.A., Medina-Acosta E., O'Connor J., Russell D.G., Cross G.A.M. Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO LEU-122.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M94364 Genomic DNA. Translation: AAA30319.1. M94365 Genomic DNA. Translation: AAA30320.2.
PIR	A60961.
3D structure databases
ModBase	Search...
Protein family/group databases
MEROPS	M08.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001577. Peptidase_M8. [Graphical view]
PANTHER	PTHR10942. Peptidase_M8. 1 hit.
Pfam	PF01457. Peptidase_M8. 1 hit. [Graphical view]
PRINTS	PR00782. LSHMANOLYSIN.
PROSITE	PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GP63_CRIFA

Accession

Primary (citable) accession number: Q06031

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	October 5, 2010
Last modified:	November 16, 2011
This is version 70 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)