Reviewed,
UniProtKB/Swiss-Prot Q06031 (GP63_CRIFA)
Last modified
June 16, 2009.
Version 57.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Leishmanolysin homolog EC=3.4.24.36 Alternative name(s): Cell surface protease Major surface glycoprotein Protein gp63 | ||
| Gene names |
| ||
| Organism | Crithidia fasciculata | ||
| Taxonomic identifier | 5656 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Euglenozoa › Kinetoplastida › Trypanosomatidae › Crithidia |
Protein attributes
| Sequence length | 652 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Inferred from homology. |
General annotation (Comments)
| Function | Plays an integral role during the infection of macrophages in the mammalian host By similarity. |
| Catalytic activity | Preference for hydrophobic residues at P1 and P1' and basic residues at P2' and P3'. A model nonapeptide is cleaved at -Ala-Tyr-|-Leu-Lys-Lys-. |
| Cofactor | Binds 1 zinc ion per subunit By similarity. |
| Subcellular location | Cell membrane; Lipid-anchor › GPI-anchor By similarity. |
| Sequence similarities | Belongs to the peptidase M8 family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Cell adhesion |
| Cellular component | Cell membrane Membrane |
| Domain | Signal |
| Ligand | Metal-binding Zinc |
| Molecular function | Hydrolase Metalloprotease Protease |
| PTM | Disulfide bond GPI-anchor Glycoprotein Lipoprotein Zymogen |
| Gene Ontology (GO) | |
| Biological process | cell adhesion Inferred from electronic annotation. Source: UniProtKB-KW proteolysisInferred from electronic annotation. Source: InterPro |
| Cellular component | anchored to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membraneInferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro protein bindingInferred from electronic annotation. Source: UniProtKB-KW zinc ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||
Molecule processing | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 44 | 44 | By similarity | ||||||||
| Propeptide | 45 – 113 | 69 | Activation peptide By similarity | PRO_0000028652 | |||||||
| Chain | 114 – 627 | 514 | Leishmanolysin homolog | PRO_0000028653 | |||||||
| Propeptide | 628 – 652 | 25 | Removed in mature form Potential | PRO_0000028654 | |||||||
Sites | |||||||||||
| Active site | 276 | 1 | By similarity | ||||||||
| Metal binding | 275 | 1 | Zinc; catalytic Potential | ||||||||
| Metal binding | 279 | 1 | Zinc; catalytic Potential | ||||||||
| Metal binding | 345 | 1 | Zinc; catalytic By similarity | ||||||||
Amino acid modifications | |||||||||||
| Lipidation | 627 | 1 | GPI-anchor amidated serine Potential | ||||||||
| Glycosylation | 382 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 408 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 568 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Disulfide bond | 137 ↔ 154 | By similarity | |||||||||
| Disulfide bond | 202 ↔ 241 | By similarity | |||||||||
| Disulfide bond | 325 ↔ 397 | By similarity | |||||||||
| Disulfide bond | 404 ↔ 467 | By similarity | |||||||||
| Disulfide bond | 417 ↔ 436 | By similarity | |||||||||
| Disulfide bond | 426 ↔ 501 | By similarity | |||||||||
| Disulfide bond | 478 ↔ 523 | By similarity | |||||||||
| Disulfide bond | 528 ↔ 578 | By similarity | |||||||||
| Disulfide bond | 548 ↔ 571 | By similarity | |||||||||
Sequences
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References
| [1] | "Crithidia fasciculata contains a transcribed leishmanial surface proteinase (gp63) gene homologue." Inverso J.A., Medina-Acosta E., O'Connor J., Russell D.G., Cross G.A.M. Mol. Biochem. Parasitol. 57:47-54(1993) [PubMed: 8426615] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 11745. |
Cross-references
Sequence databases | |
|---|---|
| M94364 Genomic DNA. Translation: AAA30319.1. M94365 Genomic DNA. Translation: AAA30320.1. | |
| PIR | A60961. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1LML based on UniProtKB P08148. |
| ModBase | Search... |
Protein family/group databases | |
| MEROPS | M08.001. |
Enzyme and pathway databases | |
| BRENDA | 3.4.24.36. 571. |
Family and domain databases | |
| InterPro | IPR006025. Pept_M_Zn_BS. IPR001577. Peptidase_M8. [Graphical view] |
| PANTHER | PTHR10942. Peptidase_M8. 1 hit. |
| Pfam | PF01457. Peptidase_M8. 1 hit. [Graphical view] |
| PRINTS | PR00782. LSHMANOLYSIN. |
| PROSITE | PS00142. ZINC_PROTEASE. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | GP63_CRIFA | ||||||||
| Accession | Primary (citable) accession number: Q06031 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
Relevant documents
| Peptidase families Classification of peptidase families and list of entries |
| SIMILARITY comments Index of protein domains and families |

Clusters with


