Reviewed, UniProtKB/Swiss-Prot Q06031 (GP63_CRIFA)

Names and origin

Protein names

Recommended name:
    Leishmanolysin homolog
    EC=3.4.24.36
Alternative name(s):
    Cell surface protease
    Major surface glycoprotein
    Protein gp63

Gene names

Name:

gp63

Organism

Crithidia fasciculata

Taxonomic identifier

5656 [NCBI]

Taxonomic lineage

Eukaryota › Euglenozoa › Kinetoplastida › Trypanosomatidae › Crithidia

Protein attributes

Sequence length	652 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Plays an integral role during the infection of macrophages in the mammalian host By similarity.
Catalytic activity	Preference for hydrophobic residues at P1 and P1' and basic residues at P2' and P3'. A model nonapeptide is cleaved at -Ala-Tyr-|-Leu-Lys-Lys-.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subcellular location	Cell membrane; Lipid-anchor › GPI-anchor By similarity.
Sequence similarities	Belongs to the peptidase M8 family.

Ontologies

Keywords
Biological process	Cell adhesion
Cellular component	Cell membrane Membrane
Domain	Signal
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
PTM	Disulfide bond GPI-anchor Glycoprotein Lipoprotein Zymogen
Gene Ontology (GO)
Biological process	cell adhesion Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	anchored to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro protein binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 44	44	By similarity
	Propeptide	45 – 113	69	Activation peptide By similarity		PRO_0000028652
	Chain	114 – 627	514	Leishmanolysin homolog		PRO_0000028653
	Propeptide	628 – 652	25	Removed in mature form Potential		PRO_0000028654
Sites
	Active site	276	1	By similarity
	Metal binding	275	1	Zinc; catalytic Potential
	Metal binding	279	1	Zinc; catalytic Potential
	Metal binding	345	1	Zinc; catalytic By similarity
Amino acid modifications
	Lipidation	627	1	GPI-anchor amidated serine Potential
	Glycosylation	382	1	N-linked (GlcNAc...) Potential
	Glycosylation	408	1	N-linked (GlcNAc...) Potential
	Glycosylation	568	1	N-linked (GlcNAc...) Potential
	Disulfide bond	137 ↔ 154		By similarity
	Disulfide bond	202 ↔ 241		By similarity
	Disulfide bond	325 ↔ 397		By similarity
	Disulfide bond	404 ↔ 467		By similarity
	Disulfide bond	417 ↔ 436		By similarity
	Disulfide bond	426 ↔ 501		By similarity
	Disulfide bond	478 ↔ 523		By similarity
	Disulfide bond	528 ↔ 578		By similarity
	Disulfide bond	548 ↔ 571		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q06031-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 9B02FE3A283F4119	FASTA	652	69,058
	10 20 30 40 50 60 MHAPPTATRR SGPRRTHGIM ARLVRLAAGV LVVTLVIGAL TALSADDAKT HPHKVCIHDE 70 80 90 100 110 120 LQQSLLDSVA QQGLAPQRVS RVGLPYVASA TAAPAAQVGG VDFALAGDSA PDVTRSAEWG 130 140 150 160 170 180 ERITVSAEEL TDPAYHCATV GQVISNHIDD YVTCTADDIM TAEKLDILMN YLIPEALQMH 190 200 210 220 230 240 KDRLQVQQVQ GTWKVARMTS YCGRFKVPEE HFTTGLSNTD FVLYVASVPT SPGVLAWANT 250 260 270 280 290 300 CQVFSNDQPA VGVINIPAAT ITERYDHLMV HAVTHEIAHS LGFSNAFFTN TGIGQFVTGV 310 320 330 340 350 360 RGNPDTVPVI NSPTVVAKAR EHYGCDDVTY VELEDAGGSG TMGSHWKIRN AQDELMAGIS 370 380 390 400 410 420 GVAYYTSLTL SAFEDLGYYK ANYSNAETMK WGKDVGCAFL TGKCVVDNVT QFPSMYCDKD 430 440 450 460 470 480 ENVYRCHTAR LNLGSCEVTD YTFDLPDYLQ YFTVPSVGGS ADYYDYCPYI VRSPIGSCTQ 490 500 510 520 530 540 AASSASPFVS AFNTFSMASR CIDGTFTPKS TGGATVTAHL GMCTNVACNT ADKTYSIQVY 550 560 570 580 590 600 GNGAYIPCTP GATISLDTVS DAFEAGGNIT CPPYLEVCQS NVKGAMDYES MTNSGSGSSR 610 620 630 640 650 PAPVEPSGSG SGSSAATTAP SPTRDGSAAA DRIAPRTAAV ALLALAVAAA CV

References

[1]

"Crithidia fasciculata contains a transcribed leishmanial surface proteinase (gp63) gene homologue." Inverso J.A., Medina-Acosta E., O'Connor J., Russell D.G., Cross G.A.M. Mol. Biochem. Parasitol. 57:47-54(1993) [PubMed: 8426615] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 11745.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M94364 Genomic DNA. Translation: AAA30319.1. M94365 Genomic DNA. Translation: AAA30320.1.
PIR	A60961.
3D structure databases
HSSP	HSSP built from PDB template 1LML based on UniProtKB P08148.
ModBase	Search...
Protein family/group databases
MEROPS	M08.001.
Enzyme and pathway databases
BRENDA	3.4.24.36. 571.
Family and domain databases
InterPro	IPR006025. Pept_M_Zn_BS. IPR001577. Peptidase_M8. [Graphical view]
PANTHER	PTHR10942. Peptidase_M8. 1 hit.
Pfam	PF01457. Peptidase_M8. 1 hit. [Graphical view]
PRINTS	PR00782. LSHMANOLYSIN.
PROSITE	PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GP63_CRIFA

Accession

Primary (citable) accession number: Q06031

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	June 16, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)