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Q06006 (NIR_PSECL) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Copper-containing nitrite reductase

EC=1.7.2.1
Alternative name(s):
Cu-NIR
Gene names
Name:nirK
OrganismPseudomonas chlororaphis (Pseudomonas aureofaciens)
Taxonomic identifier333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length363 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Nitric oxide + H2O + ferricytochrome c = nitrite + ferrocytochrome c + 2 H+.

Cofactor

Binds 1 Cu2+ ion. The Cu2+ ion is held by residues from each of 2 monomers of the trimer. Nitrite is bound to the Cu2+ ion site. Pseudoazurin is the physiological electron donor for the Cu-NIR in vitro.

Binds 1 Cu+ ion. The Cu+ ion is bound within a single monomer.

FAD.

Pathway

Nitrogen metabolism; nitrate reduction (denitrification); dinitrogen from nitrate: step 2/4.

Subunit structure

Homotrimer By similarity.

Subcellular location

Periplasm.

Domain

The type I copper site in NIR plays a crucial role for electron transfer from pseudoazurin to the type II copper site of NIR, which comprises the catalytic center of NIR for the reduction of nitrite.

Sequence similarities

Belongs to the multicopper oxidase family.

Contains 2 plastocyanin-like domains.

Ontologies

Keywords
   Biological processNitrate assimilation
   Cellular componentPeriplasm
   DomainRepeat
Signal
   LigandCopper
FAD
Flavoprotein
Metal-binding
   Molecular functionOxidoreductase
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological_processdenitrification pathway

Inferred from electronic annotation. Source: UniProtKB-UniPathway

nitrate assimilation

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentperiplasmic space

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functioncopper ion binding

Inferred from electronic annotation. Source: InterPro

nitrite reductase (NO-forming) activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Ref.1
Chain25 – 363339Copper-containing nitrite reductase
PRO_0000002988

Regions

Domain25 – 193169Plastocyanin-like 1
Domain194 – 363170Plastocyanin-like 2

Sites

Metal binding1131Copper 1; type 1 By similarity
Metal binding1181Copper 2; type 2 By similarity
Metal binding1531Copper 2; type 2 By similarity
Metal binding1541Copper 1; type 1 By similarity
Metal binding1631Copper 1; type 1 By similarity
Metal binding1681Copper 1; type 1 By similarity
Metal binding3241Copper 2; type 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q06006 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 50DDB60CC4DC3E00

FASTA36339,248
        10         20         30         40         50         60 
MSVFRSVLGA CVLLGSCASS LALAGGAEGL QRVKVDLVAP PLVHPHEQVV SGPPKVVQFR 

        70         80         90        100        110        120 
MSIEEKKMVI DDQGTTLQAM TFNGSMPGPT LVVHEGDYIE LTLVNPATNS MPHNVDFHAA 

       130        140        150        160        170        180 
TGALGGAGLT QVVPGQEVVL RFKADRSGTF VYHCAPQGMV PWHVVSGMNG ALMVLPRDGL 

       190        200        210        220        230        240 
RDPQGKLLHY DRVYTIGESD LYIPKDKDGH YKDYPDLASS YQDTRAVMRT LTPSHVVFNG 

       250        260        270        280        290        300 
RVGALTGANA LTSKVGESVL FIHSQANRDS RPHLIGGHGD WVWTTGKFAN PPQRNMETWF 

       310        320        330        340        350        360 
IPGGSAVAAL YTFKQPGTYV YLSHNLIEAM ELGALAQIKV EGQWDDDLMT QVKAPGPIVE 


PKQ 

« Hide

References

[1]"Copper-containing nitrite reductase from Pseudomonas aureofaciens is functional in a mutationally cytochrome cd1-free background (NirS-) of Pseudomonas stutzeri."
Glockner A.B., Juengst A., Zumft W.G.
Arch. Microbiol. 160:18-26(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 25-41.
Strain: ATCC 13985 / DSM 50082 / NRRL B-1576 / Stanier 38.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z21945 Genomic DNA. Translation: CAA79939.1.
PIRS32112.

3D structure databases

ProteinModelPortalQ06006.
SMRQ06006. Positions 27-358.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00652; UER00707.

Family and domain databases

Gene3D2.60.40.420. 2 hits.
InterProIPR001117. Cu-oxidase.
IPR011707. Cu-oxidase_3.
IPR008972. Cupredoxin.
IPR001287. NO2-reductase_Cu.
[Graphical view]
PfamPF00394. Cu-oxidase. 1 hit.
PF07732. Cu-oxidase_3. 1 hit.
[Graphical view]
PRINTSPR00695. CUNO2RDTASE.
SUPFAMSSF49503. SSF49503. 2 hits.
TIGRFAMsTIGR02376. Cu_nitrite_red. 1 hit.
ProtoNetSearch...

Entry information

Entry nameNIR_PSECL
AccessionPrimary (citable) accession number: Q06006
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: October 16, 2013
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways