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Q06006

- NIR_PSECL

UniProt

Q06006 - NIR_PSECL

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Protein

Copper-containing nitrite reductase

Gene

nirK

Organism
Pseudomonas chlororaphis (Pseudomonas aureofaciens)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

Nitric oxide + H2O + ferricytochrome c = nitrite + ferrocytochrome c + 2 H+.

Cofactori

Binds 1 Cu2+ ion. The Cu2+ ion is held by residues from each of 2 monomers of the trimer. Nitrite is bound to the Cu2+ ion site. Pseudoazurin is the physiological electron donor for the Cu-NIR in vitro.
Binds 1 Cu+ ion. The Cu+ ion is bound within a single monomer.
FAD.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi113 – 1131Copper 1; type 1By similarity
Metal bindingi118 – 1181Copper 2; type 2By similarity
Metal bindingi153 – 1531Copper 2; type 2By similarity
Metal bindingi154 – 1541Copper 1; type 1By similarity
Metal bindingi163 – 1631Copper 1; type 1By similarity
Metal bindingi168 – 1681Copper 1; type 1By similarity
Metal bindingi324 – 3241Copper 2; type 2By similarity

GO - Molecular functioni

  1. copper ion binding Source: InterPro
  2. nitrite reductase (NO-forming) activity Source: UniProtKB-EC

GO - Biological processi

  1. denitrification pathway Source: UniProtKB-UniPathway
  2. nitrate assimilation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Nitrate assimilation

Keywords - Ligandi

Copper, FAD, Flavoprotein, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00652; UER00707.

Names & Taxonomyi

Protein namesi
Recommended name:
Copper-containing nitrite reductase (EC:1.7.2.1)
Alternative name(s):
Cu-NIR
Gene namesi
Name:nirK
OrganismiPseudomonas chlororaphis (Pseudomonas aureofaciens)
Taxonomic identifieri333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Subcellular locationi

GO - Cellular componenti

  1. periplasmic space Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 24241 PublicationAdd
BLAST
Chaini25 – 363339Copper-containing nitrite reductasePRO_0000002988Add
BLAST

Interactioni

Subunit structurei

Homotrimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ06006.
SMRiQ06006. Positions 27-358.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini25 – 193169Plastocyanin-like 1Add
BLAST
Domaini194 – 363170Plastocyanin-like 2Add
BLAST

Domaini

The type I copper site in NIR plays a crucial role for electron transfer from pseudoazurin to the type II copper site of NIR, which comprises the catalytic center of NIR for the reduction of nitrite.

Sequence similaritiesi

Belongs to the multicopper oxidase family.Curated
Contains 2 plastocyanin-like domains.Curated

Keywords - Domaini

Repeat, Signal

Family and domain databases

Gene3Di2.60.40.420. 2 hits.
InterProiIPR001117. Cu-oxidase.
IPR011707. Cu-oxidase_3.
IPR008972. Cupredoxin.
IPR001287. NO2-reductase_Cu.
[Graphical view]
PfamiPF00394. Cu-oxidase. 1 hit.
PF07732. Cu-oxidase_3. 1 hit.
[Graphical view]
PRINTSiPR00695. CUNO2RDTASE.
SUPFAMiSSF49503. SSF49503. 2 hits.
TIGRFAMsiTIGR02376. Cu_nitrite_red. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q06006-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSVFRSVLGA CVLLGSCASS LALAGGAEGL QRVKVDLVAP PLVHPHEQVV
60 70 80 90 100
SGPPKVVQFR MSIEEKKMVI DDQGTTLQAM TFNGSMPGPT LVVHEGDYIE
110 120 130 140 150
LTLVNPATNS MPHNVDFHAA TGALGGAGLT QVVPGQEVVL RFKADRSGTF
160 170 180 190 200
VYHCAPQGMV PWHVVSGMNG ALMVLPRDGL RDPQGKLLHY DRVYTIGESD
210 220 230 240 250
LYIPKDKDGH YKDYPDLASS YQDTRAVMRT LTPSHVVFNG RVGALTGANA
260 270 280 290 300
LTSKVGESVL FIHSQANRDS RPHLIGGHGD WVWTTGKFAN PPQRNMETWF
310 320 330 340 350
IPGGSAVAAL YTFKQPGTYV YLSHNLIEAM ELGALAQIKV EGQWDDDLMT
360
QVKAPGPIVE PKQ
Length:363
Mass (Da):39,248
Last modified:February 1, 1995 - v1
Checksum:i50DDB60CC4DC3E00
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z21945 Genomic DNA. Translation: CAA79939.1.
PIRiS32112.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z21945 Genomic DNA. Translation: CAA79939.1 .
PIRi S32112.

3D structure databases

ProteinModelPortali Q06006.
SMRi Q06006. Positions 27-358.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00652 ; UER00707 .

Family and domain databases

Gene3Di 2.60.40.420. 2 hits.
InterProi IPR001117. Cu-oxidase.
IPR011707. Cu-oxidase_3.
IPR008972. Cupredoxin.
IPR001287. NO2-reductase_Cu.
[Graphical view ]
Pfami PF00394. Cu-oxidase. 1 hit.
PF07732. Cu-oxidase_3. 1 hit.
[Graphical view ]
PRINTSi PR00695. CUNO2RDTASE.
SUPFAMi SSF49503. SSF49503. 2 hits.
TIGRFAMsi TIGR02376. Cu_nitrite_red. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Copper-containing nitrite reductase from Pseudomonas aureofaciens is functional in a mutationally cytochrome cd1-free background (NirS-) of Pseudomonas stutzeri."
    Glockner A.B., Juengst A., Zumft W.G.
    Arch. Microbiol. 160:18-26(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 25-41.
    Strain: ATCC 13985 / DSM 50082 / NRRL B-1576 / Stanier 38.

Entry informationi

Entry nameiNIR_PSECL
AccessioniPrimary (citable) accession number: Q06006
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: October 29, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3