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Q06006

- NIR_PSECL

UniProt

Q06006 - NIR_PSECL

Protein

Copper-containing nitrite reductase

Gene

nirK

Organism
Pseudomonas chlororaphis (Pseudomonas aureofaciens)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Nitric oxide + H2O + ferricytochrome c = nitrite + ferrocytochrome c + 2 H+.

    Cofactori

    Binds 1 Cu2+ ion. The Cu2+ ion is held by residues from each of 2 monomers of the trimer. Nitrite is bound to the Cu2+ ion site. Pseudoazurin is the physiological electron donor for the Cu-NIR in vitro.
    Binds 1 Cu+ ion. The Cu+ ion is bound within a single monomer.
    FAD.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi113 – 1131Copper 1; type 1By similarity
    Metal bindingi118 – 1181Copper 2; type 2By similarity
    Metal bindingi153 – 1531Copper 2; type 2By similarity
    Metal bindingi154 – 1541Copper 1; type 1By similarity
    Metal bindingi163 – 1631Copper 1; type 1By similarity
    Metal bindingi168 – 1681Copper 1; type 1By similarity
    Metal bindingi324 – 3241Copper 2; type 2By similarity

    GO - Molecular functioni

    1. copper ion binding Source: InterPro
    2. nitrite reductase (NO-forming) activity Source: UniProtKB-EC

    GO - Biological processi

    1. denitrification pathway Source: UniProtKB-UniPathway
    2. nitrate assimilation Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Nitrate assimilation

    Keywords - Ligandi

    Copper, FAD, Flavoprotein, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00652; UER00707.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Copper-containing nitrite reductase (EC:1.7.2.1)
    Alternative name(s):
    Cu-NIR
    Gene namesi
    Name:nirK
    OrganismiPseudomonas chlororaphis (Pseudomonas aureofaciens)
    Taxonomic identifieri333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

    Subcellular locationi

    GO - Cellular componenti

    1. periplasmic space Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Periplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 24241 PublicationAdd
    BLAST
    Chaini25 – 363339Copper-containing nitrite reductasePRO_0000002988Add
    BLAST

    Interactioni

    Subunit structurei

    Homotrimer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ06006.
    SMRiQ06006. Positions 27-358.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini25 – 193169Plastocyanin-like 1Add
    BLAST
    Domaini194 – 363170Plastocyanin-like 2Add
    BLAST

    Domaini

    The type I copper site in NIR plays a crucial role for electron transfer from pseudoazurin to the type II copper site of NIR, which comprises the catalytic center of NIR for the reduction of nitrite.

    Sequence similaritiesi

    Belongs to the multicopper oxidase family.Curated
    Contains 2 plastocyanin-like domains.Curated

    Keywords - Domaini

    Repeat, Signal

    Family and domain databases

    Gene3Di2.60.40.420. 2 hits.
    InterProiIPR001117. Cu-oxidase.
    IPR011707. Cu-oxidase_3.
    IPR008972. Cupredoxin.
    IPR001287. NO2-reductase_Cu.
    [Graphical view]
    PfamiPF00394. Cu-oxidase. 1 hit.
    PF07732. Cu-oxidase_3. 1 hit.
    [Graphical view]
    PRINTSiPR00695. CUNO2RDTASE.
    SUPFAMiSSF49503. SSF49503. 2 hits.
    TIGRFAMsiTIGR02376. Cu_nitrite_red. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q06006-1 [UniParc]FASTAAdd to Basket

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    MSVFRSVLGA CVLLGSCASS LALAGGAEGL QRVKVDLVAP PLVHPHEQVV    50
    SGPPKVVQFR MSIEEKKMVI DDQGTTLQAM TFNGSMPGPT LVVHEGDYIE 100
    LTLVNPATNS MPHNVDFHAA TGALGGAGLT QVVPGQEVVL RFKADRSGTF 150
    VYHCAPQGMV PWHVVSGMNG ALMVLPRDGL RDPQGKLLHY DRVYTIGESD 200
    LYIPKDKDGH YKDYPDLASS YQDTRAVMRT LTPSHVVFNG RVGALTGANA 250
    LTSKVGESVL FIHSQANRDS RPHLIGGHGD WVWTTGKFAN PPQRNMETWF 300
    IPGGSAVAAL YTFKQPGTYV YLSHNLIEAM ELGALAQIKV EGQWDDDLMT 350
    QVKAPGPIVE PKQ 363
    Length:363
    Mass (Da):39,248
    Last modified:February 1, 1995 - v1
    Checksum:i50DDB60CC4DC3E00
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z21945 Genomic DNA. Translation: CAA79939.1.
    PIRiS32112.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z21945 Genomic DNA. Translation: CAA79939.1 .
    PIRi S32112.

    3D structure databases

    ProteinModelPortali Q06006.
    SMRi Q06006. Positions 27-358.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00652 ; UER00707 .

    Family and domain databases

    Gene3Di 2.60.40.420. 2 hits.
    InterProi IPR001117. Cu-oxidase.
    IPR011707. Cu-oxidase_3.
    IPR008972. Cupredoxin.
    IPR001287. NO2-reductase_Cu.
    [Graphical view ]
    Pfami PF00394. Cu-oxidase. 1 hit.
    PF07732. Cu-oxidase_3. 1 hit.
    [Graphical view ]
    PRINTSi PR00695. CUNO2RDTASE.
    SUPFAMi SSF49503. SSF49503. 2 hits.
    TIGRFAMsi TIGR02376. Cu_nitrite_red. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Copper-containing nitrite reductase from Pseudomonas aureofaciens is functional in a mutationally cytochrome cd1-free background (NirS-) of Pseudomonas stutzeri."
      Glockner A.B., Juengst A., Zumft W.G.
      Arch. Microbiol. 160:18-26(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 25-41.
      Strain: ATCC 13985 / DSM 50082 / NRRL B-1576 / Stanier 38.

    Entry informationi

    Entry nameiNIR_PSECL
    AccessioniPrimary (citable) accession number: Q06006
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 80 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3