ID   ASSY_CARRP              Reviewed;         393 AA.
AC   Q05FN6;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   14-NOV-2006, sequence version 1.
DT   16-JUN-2009, entry version 15.
DE   RecName: Full=Argininosuccinate synthase;
DE            EC=6.3.4.5;
DE   AltName: Full=Citrulline--aspartate ligase;
GN   Name=argG; OrderedLocusNames=CRP_104;
OS   Carsonella ruddii (strain PV).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Candidatus Carsonella.
OX   NCBI_TaxID=387662;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=17038615; DOI=10.1126/science.1134196;
RA   Nakabachi A., Yamashita A., Toh H., Ishikawa H., Dunbar H.E.,
RA   Moran N.A., Hattori M.;
RT   "The 160-kilobase genome of the bacterial endosymbiont Carsonella.";
RL   Science 314:267-267(2006).
CC   -!- CATALYTIC ACTIVITY: ATP + L-citrulline + L-aspartate = AMP +
CC       diphosphate + N(omega)-(L-arginino)succinate.
CC   -!- PATHWAY: Amino-acid biosynthesis; L-arginine biosynthesis; L-
CC       arginine from L-ornithine and carbamoyl phosphate: step 2/3.
CC   -!- SUBUNIT: Homotetramer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (Probable).
CC   -!- SIMILARITY: Belongs to the argininosuccinate synthase family. Type
CC       1 subfamily.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; AP009180; BAF35135.1; -; Genomic_DNA.
DR   RefSeq; YP_802501.1; -.
DR   GeneID; 4414925; -.
DR   GenomeReviews; AP009180_GR; CRP_104.
DR   KEGG; crp:CRP_104; -.
DR   OMA; Q05FN6; YVFPMFR.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004055; F:argininosuccinate synthase activity; IEA:HAMAP.
DR   GO; GO:0005524; F:ATP binding; IEA:HAMAP.
DR   GO; GO:0006526; P:arginine biosynthetic process; IEA:HAMAP.
DR   HAMAP; MF_00005; -; 1.
DR   InterPro; IPR001518; Arginosuc_synth.
DR   InterPro; IPR018223; Arginosuc_synth_CS.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   Gene3D; G3DSA:3.40.50.620; Rossmann-like_a/b/a_fold; 1.
DR   PANTHER; PTHR11587; Arginosuc_synth; 1.
DR   Pfam; PF00764; Arginosuc_synth; 1.
DR   TIGRFAMs; TIGR00032; argG; 1.
DR   PROSITE; PS00564; ARGININOSUCCIN_SYN_1; 1.
DR   PROSITE; PS00565; ARGININOSUCCIN_SYN_2; 1.
PE   3: Inferred from homology;
KW   Amino-acid biosynthesis; Arginine biosynthesis; ATP-binding;
KW   Complete proteome; Cytoplasm; Ligase; Nucleotide-binding.
FT   CHAIN         1    393       Argininosuccinate synthase.
FT                                /FTId=PRO_1000000390.
FT   NP_BIND      10     18       ATP (By similarity).
FT   BINDING      37     37       ATP; via amide nitrogen and carbonyl
FT                                oxygen (By similarity).
FT   BINDING      88     88       Citrulline (By similarity).
FT   BINDING     118    118       ATP; via amide nitrogen (By similarity).
FT   BINDING     120    120       Aspartate (By similarity).
FT   BINDING     124    124       Aspartate (By similarity).
FT   BINDING     124    124       Citrulline (By similarity).
FT   BINDING     125    125       Aspartate (By similarity).
FT   BINDING     128    128       Citrulline (By similarity).
FT   BINDING     176    176       Citrulline (By similarity).
FT   BINDING     185    185       Citrulline (By similarity).
FT   BINDING     261    261       Citrulline (By similarity).
FT   BINDING     273    273       Citrulline (By similarity).
SQ   SEQUENCE   393 AA;  45320 MW;  1FDB21D282CD12F7 CRC64;
     MKIKEKIVLA YSGGLDTSVI VKWLQNELNF EVITFTADLG QGEEILLAKK KAKLLNIKNI
     FVKNLKKEFI KNFVFPFLRS SSTYENNYLL GTAIARPLIV KELMKISYYL NTNYVSHGAT
     GKGNDQIRFE LGFKYFNPKI KIIAPWRIWN LNSRNSLLNF CIKNNIKFDS KTKKYSIDKN
     LFHNSYEGGN LDNINYEPDE PMWEHTLSNY NSLDYPIYIS LTFKNGDPIK INNKNYNVEE
     LFLKLNNLGS IAGIGRLDII ENRLIGIKSR GCYESPGASI IMYARKKLES LILDKEIYSF
     KEEIALKYSK LVYNGYWWSP ERILLQKIID YTQTSINGII KLKIFKGQIN IASINSINSL
     FNSKNSSFDE ISNLFNQSDS SGFINIKSLR LII
//