SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q05D44

- IF2P_MOUSE

UniProt

Q05D44 - IF2P_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Eukaryotic translation initiation factor 5B

Gene
Eif5b, If2
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Function in general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes. Seems to function along with eIF-2 By similarity.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi634 – 6418GTP By similarity

GO - Molecular functioni

  1. GTPase activity Source: InterPro
  2. GTP binding Source: UniProtKB-KW
  3. translation initiation factor activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Initiation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 5B
Short name:
eIF-5B
Alternative name(s):
Translation initiation factor IF-2
Gene namesi
Name:Eif5b
Synonyms:If2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 1

Organism-specific databases

MGIiMGI:2441772. Eif5b.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12161216Eukaryotic translation initiation factor 5BPRO_0000354071Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei108 – 1081Phosphoserine By similarity
Modified residuei114 – 1141Phosphoserine2 Publications
Modified residuei137 – 1371Phosphoserine3 Publications
Modified residuei139 – 1391Phosphoserine3 Publications
Modified residuei165 – 1651Phosphoserine1 Publication
Modified residuei172 – 1721Phosphoserine By similarity
Modified residuei183 – 1831Phosphoserine By similarity
Modified residuei184 – 1841Phosphoserine By similarity
Modified residuei187 – 1871Phosphoserine2 Publications
Modified residuei191 – 1911Phosphoserine2 Publications
Modified residuei215 – 2151Phosphoserine4 Publications
Modified residuei300 – 3001Phosphothreonine By similarity
Modified residuei497 – 4971Phosphothreonine By similarity
Modified residuei556 – 5561Phosphoserine By similarity
Modified residuei584 – 5841Phosphoserine By similarity
Modified residuei585 – 5851Phosphoserine By similarity
Modified residuei587 – 5871Phosphoserine By similarity
Modified residuei591 – 5911Phosphoserine By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ05D44.
PaxDbiQ05D44.
PRIDEiQ05D44.

PTM databases

PhosphoSiteiQ05D44.

Expressioni

Gene expression databases

ArrayExpressiQ05D44.
BgeeiQ05D44.
GenevestigatoriQ05D44.

Interactioni

Subunit structurei

Interacts with ANXA5 in a calcium and phospholipid-dependent manner By similarity.

Protein-protein interaction databases

BioGridi230580. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ05D44.
SMRiQ05D44. Positions 583-1195.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini625 – 842218tr-type GAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni634 – 6418G1 By similarity
Regioni659 – 6635G2 By similarity
Regioni698 – 7014G3 By similarity
Regioni752 – 7554G4 By similarity
Regioni820 – 8223G5 By similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi39 – 452414Lys-richAdd
BLAST
Compositional biasi235 – 561327Glu-richAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0532.
GeneTreeiENSGT00730000111064.
HOVERGENiHBG019036.
InParanoidiQ8CFF4.
KOiK03243.
OMAiGSLRMHT.
OrthoDBiEOG7034GC.
PhylomeDBiQ05D44.
TreeFamiTF101535.

Family and domain databases

Gene3Di3.40.50.10050. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR000795. EF_GTP-bd_dom.
IPR029459. EFTU-type.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR023115. TIF_IF2_dom3.
IPR009000. Transl_B-barrel.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view]
PfamiPF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF14578. GTP_EFTU_D4. 1 hit.
PF11987. IF-2. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF52156. SSF52156. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q05D44-1 [UniParc]FASTAAdd to Basket

« Hide

MGKKQKNKSE DSTKDDTDLG ALAAEIEGAG AAKEQEPQKS KGKKKKEKKK     50
QDFDENDILR ELEELSLEAQ GIRADRDAAA VKPTENNEEE SASKQDKKKK 100
GQKGKKTSFD ENDSEELEDK DSKSKKTARP NSEAPLSGSE DADDSNKLSK 150
KGKKAQKSTK KRDGSEEDED NSKRSKERSR VNSSGESGGE SDEFLQSRKG 200
QKKNQKNKSV PTVDSGNEDD DSSFKIKTVA QKKAEKKERE KKKRDEEKAK 250
LRKMKEKEEL EKGKKEQSKQ REPQKRPEEE VLTLRGTPDT GAASEEKGDT 300
AAALEDDNEG DKKKKDKKKK KTEKDEKEKE KKKGPSKSTV KAIQEALAKL 350
KEEEERQKRE EEERIKRLEE LEAKRKEEER LEQEKRERKK QKEKERKERL 400
KKEGKLLTKS QREARARAEV TLRHLQAQGV EVPSKDSLPK KRPVYEDKKK 450
KKTPQQLESK EVSETLEISA PVEAVDQGGP EKEETPPSVE PEEEEDTEDA 500
GLDDWEAMAS DEEREKEGNM IHIEVEENPE EEEEEEEEEE EEESEDEEEE 550
GDSEGSDGDE EDCKLSDEKD SGKAGDTKPS KDASSDSEYD SDDDRTKEER 600
AYDKAKRRIE KRRLEHGKNV NTEKLRAPII CVLGHVDTGK TKILDKLRHT 650
HVQDGEAGGI TQQIGATNVP LEAINEQTKM IKNFDRENVR IPGMLIIDTP 700
GHESFSNLRN RGSSLCDIAI LVVDIMHGLE PQTIESINIL KSKKCPFIVA 750
LNKIDRLYDW KKSPDSDVAV TLKKQKKNTK DEFEERAKAI IVEFAQQGLN 800
AALFYENKDP RTFVSLVPTS AHTGDGMGSL IYLLVELTQT MLSKRLAHCE 850
ELRAQVMEVK ALPGMGTTID VILINGRLKE GDTIIVPGVE GPIVTQIRGL 900
LLPPPMKELR VKNQYEKHKE VEAAQGVKIL GKDLEKTLAG LPLLVAYKDD 950
EIPVLKDELI HELKQTLNAI KLEEKGVYVQ ASTLGSLEAL LEFLKTSEVP 1000
YAGINIGPVH KKDVMKASVM LEHDPQYAVI LAFDVRIERD AQEMADSLGV 1050
RIFSAEIIYH LFDAFTKYRQ DYKKQKQEEF KHIAVFPCKM KILPQYIFNS 1100
RDPIVIGVTV EAGQVKQGTP MCVPSKNFVD IGIVTSIEIN HKQVDVAKKG 1150
QEVCVKIEPI PGESPKMFGR HFEATDILVS KISRQSIDAL KDWFRDEMQK 1200
SDWQLIVELK KVFEII 1216
Length:1,216
Mass (Da):137,616
Last modified:November 25, 2008 - v2
Checksum:i38D1C21648E4EAEC
GO

Sequence cautioni

The sequence AAH18347.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.
The sequence AAH37150.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.
The sequence AAH40746.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.
The sequence AAH60288.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti121 – 1211D → N in BAE26259. 1 Publication
Sequence conflicti188 – 1881G → C in BAE26259. 1 Publication
Sequence conflicti254 – 2541M → L in BAE26259. 1 Publication
Sequence conflicti302 – 3021A → V in BAE26259. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC114583 Genomic DNA. No translation available.
AK145146 mRNA. Translation: BAE26259.1.
AK145732 mRNA. Translation: BAE26614.1.
AK163527 mRNA. Translation: BAE37384.1.
BC018347 mRNA. Translation: AAH18347.1. Sequence problems.
BC037150 mRNA. Translation: AAH37150.1. Sequence problems.
BC040746 mRNA. Translation: AAH40746.1. Sequence problems.
BC060288 mRNA. Translation: AAH60288.1. Sequence problems.
CCDSiCCDS35544.1.
RefSeqiNP_938045.2. NM_198303.2.
UniGeneiMm.260943.

Genome annotation databases

EnsembliENSMUST00000027252; ENSMUSP00000027252; ENSMUSG00000026083.
GeneIDi226982.
KEGGimmu:226982.
UCSCiuc007aso.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC114583 Genomic DNA. No translation available.
AK145146 mRNA. Translation: BAE26259.1 .
AK145732 mRNA. Translation: BAE26614.1 .
AK163527 mRNA. Translation: BAE37384.1 .
BC018347 mRNA. Translation: AAH18347.1 . Sequence problems.
BC037150 mRNA. Translation: AAH37150.1 . Sequence problems.
BC040746 mRNA. Translation: AAH40746.1 . Sequence problems.
BC060288 mRNA. Translation: AAH60288.1 . Sequence problems.
CCDSi CCDS35544.1.
RefSeqi NP_938045.2. NM_198303.2.
UniGenei Mm.260943.

3D structure databases

ProteinModelPortali Q05D44.
SMRi Q05D44. Positions 583-1195.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 230580. 1 interaction.

PTM databases

PhosphoSitei Q05D44.

Proteomic databases

MaxQBi Q05D44.
PaxDbi Q05D44.
PRIDEi Q05D44.

Protocols and materials databases

DNASUi 226982.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000027252 ; ENSMUSP00000027252 ; ENSMUSG00000026083 .
GeneIDi 226982.
KEGGi mmu:226982.
UCSCi uc007aso.1. mouse.

Organism-specific databases

CTDi 9669.
MGIi MGI:2441772. Eif5b.

Phylogenomic databases

eggNOGi COG0532.
GeneTreei ENSGT00730000111064.
HOVERGENi HBG019036.
InParanoidi Q8CFF4.
KOi K03243.
OMAi GSLRMHT.
OrthoDBi EOG7034GC.
PhylomeDBi Q05D44.
TreeFami TF101535.

Miscellaneous databases

ChiTaRSi EIF5B. mouse.
NextBioi 378430.
PROi Q05D44.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q05D44.
Bgeei Q05D44.
Genevestigatori Q05D44.

Family and domain databases

Gene3Di 3.40.50.10050. 1 hit.
3.40.50.300. 1 hit.
InterProi IPR000795. EF_GTP-bd_dom.
IPR029459. EFTU-type.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR023115. TIF_IF2_dom3.
IPR009000. Transl_B-barrel.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view ]
Pfami PF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF14578. GTP_EFTU_D4. 1 hit.
PF11987. IF-2. 1 hit.
[Graphical view ]
PRINTSi PR00315. ELONGATNFCT.
SUPFAMi SSF50447. SSF50447. 1 hit.
SSF52156. SSF52156. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsi TIGR00231. small_GTP. 1 hit.
PROSITEi PS51722. G_TR_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-771.
    Strain: C57BL/6J.
    Tissue: Corpora quadrigemina and Mammary gland.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-402.
    Strain: FVB/N.
    Tissue: Limb and Mammary tumor.
  4. "Exp5 exports eEF1A via tRNA from nuclei and synergizes with other transport pathways to confine translation to the cytoplasm."
    Bohnsack M.T., Regener K., Schwappach B., Saffrich R., Paraskeva E., Hartmann E., Goerlich D.
    EMBO J. 21:6205-6215(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-215, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic brain.
  6. "A differential phosphoproteomic analysis of retinoic acid-treated P19 cells."
    Smith J.C., Duchesne M.A., Tozzi P., Ethier M., Figeys D.
    J. Proteome Res. 6:3174-3186(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-187 AND SER-191, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Teratocarcinoma.
  7. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-114; SER-137; SER-139; SER-187; SER-191 AND SER-215, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  8. "Specific phosphopeptide enrichment with immobilized titanium ion affinity chromatography adsorbent for phosphoproteome analysis."
    Zhou H., Ye M., Dong J., Han G., Jiang X., Wu R., Zou H.
    J. Proteome Res. 7:3957-3967(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-137 AND SER-139, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  9. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-165 AND SER-215, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
    Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
    Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-114; SER-137; SER-139 AND SER-215, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiIF2P_MOUSE
AccessioniPrimary (citable) accession number: Q05D44
Secondary accession number(s): Q3SYI4
, Q3TQJ8, Q3UL37, Q3UM39, Q6PAI0, Q8CFF4, Q8CGD6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 25, 2008
Last sequence update: November 25, 2008
Last modified: September 3, 2014
This is version 64 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi