Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q05D44 (IF2P_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Eukaryotic translation initiation factor 5B

Short name=eIF-5B
Alternative name(s):
Translation initiation factor IF-2
Gene names
Name:Eif5b
Synonyms:If2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1216 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Function in general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes. Seems to function along with eIF-2 By similarity.

Subunit structure

Interacts with ANXA5 in a calcium and phospholipid-dependent manner By similarity.

Subcellular location

Cytoplasm Ref.4.

Sequence similarities

Belongs to the IF-2 family.

Sequence caution

The sequence AAH18347.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.

The sequence AAH37150.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.

The sequence AAH40746.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.

The sequence AAH60288.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandGTP-binding
Nucleotide-binding
   Molecular functionInitiation factor
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionGTP binding

Inferred from electronic annotation. Source: UniProtKB-KW

GTPase activity

Inferred from electronic annotation. Source: InterPro

translation initiation factor activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12161216Eukaryotic translation initiation factor 5B
PRO_0000354071

Regions

Nucleotide binding634 – 6418GTP By similarity
Compositional bias39 – 452414Lys-rich
Compositional bias235 – 561327Glu-rich

Amino acid modifications

Modified residue1081Phosphoserine By similarity
Modified residue1141Phosphoserine Ref.7 Ref.10
Modified residue1371Phosphoserine Ref.7 Ref.8 Ref.10
Modified residue1391Phosphoserine Ref.7 Ref.8 Ref.10
Modified residue1651Phosphoserine Ref.9
Modified residue1721Phosphoserine By similarity
Modified residue1831Phosphoserine By similarity
Modified residue1841Phosphoserine By similarity
Modified residue1871Phosphoserine Ref.6 Ref.7
Modified residue1911Phosphoserine Ref.6 Ref.7
Modified residue2151Phosphoserine Ref.5 Ref.7 Ref.9 Ref.10
Modified residue3001Phosphothreonine By similarity
Modified residue4971Phosphothreonine By similarity
Modified residue5561Phosphoserine By similarity
Modified residue5841Phosphoserine By similarity
Modified residue5851Phosphoserine By similarity
Modified residue5871Phosphoserine By similarity
Modified residue5911Phosphoserine By similarity

Experimental info

Sequence conflict1211D → N in BAE26259. Ref.2
Sequence conflict1881G → C in BAE26259. Ref.2
Sequence conflict2541M → L in BAE26259. Ref.2
Sequence conflict3021A → V in BAE26259. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q05D44 [UniParc].

Last modified November 25, 2008. Version 2.
Checksum: 38D1C21648E4EAEC

FASTA1,216137,616
        10         20         30         40         50         60 
MGKKQKNKSE DSTKDDTDLG ALAAEIEGAG AAKEQEPQKS KGKKKKEKKK QDFDENDILR 

        70         80         90        100        110        120 
ELEELSLEAQ GIRADRDAAA VKPTENNEEE SASKQDKKKK GQKGKKTSFD ENDSEELEDK 

       130        140        150        160        170        180 
DSKSKKTARP NSEAPLSGSE DADDSNKLSK KGKKAQKSTK KRDGSEEDED NSKRSKERSR 

       190        200        210        220        230        240 
VNSSGESGGE SDEFLQSRKG QKKNQKNKSV PTVDSGNEDD DSSFKIKTVA QKKAEKKERE 

       250        260        270        280        290        300 
KKKRDEEKAK LRKMKEKEEL EKGKKEQSKQ REPQKRPEEE VLTLRGTPDT GAASEEKGDT 

       310        320        330        340        350        360 
AAALEDDNEG DKKKKDKKKK KTEKDEKEKE KKKGPSKSTV KAIQEALAKL KEEEERQKRE 

       370        380        390        400        410        420 
EEERIKRLEE LEAKRKEEER LEQEKRERKK QKEKERKERL KKEGKLLTKS QREARARAEV 

       430        440        450        460        470        480 
TLRHLQAQGV EVPSKDSLPK KRPVYEDKKK KKTPQQLESK EVSETLEISA PVEAVDQGGP 

       490        500        510        520        530        540 
EKEETPPSVE PEEEEDTEDA GLDDWEAMAS DEEREKEGNM IHIEVEENPE EEEEEEEEEE 

       550        560        570        580        590        600 
EEESEDEEEE GDSEGSDGDE EDCKLSDEKD SGKAGDTKPS KDASSDSEYD SDDDRTKEER 

       610        620        630        640        650        660 
AYDKAKRRIE KRRLEHGKNV NTEKLRAPII CVLGHVDTGK TKILDKLRHT HVQDGEAGGI 

       670        680        690        700        710        720 
TQQIGATNVP LEAINEQTKM IKNFDRENVR IPGMLIIDTP GHESFSNLRN RGSSLCDIAI 

       730        740        750        760        770        780 
LVVDIMHGLE PQTIESINIL KSKKCPFIVA LNKIDRLYDW KKSPDSDVAV TLKKQKKNTK 

       790        800        810        820        830        840 
DEFEERAKAI IVEFAQQGLN AALFYENKDP RTFVSLVPTS AHTGDGMGSL IYLLVELTQT 

       850        860        870        880        890        900 
MLSKRLAHCE ELRAQVMEVK ALPGMGTTID VILINGRLKE GDTIIVPGVE GPIVTQIRGL 

       910        920        930        940        950        960 
LLPPPMKELR VKNQYEKHKE VEAAQGVKIL GKDLEKTLAG LPLLVAYKDD EIPVLKDELI 

       970        980        990       1000       1010       1020 
HELKQTLNAI KLEEKGVYVQ ASTLGSLEAL LEFLKTSEVP YAGINIGPVH KKDVMKASVM 

      1030       1040       1050       1060       1070       1080 
LEHDPQYAVI LAFDVRIERD AQEMADSLGV RIFSAEIIYH LFDAFTKYRQ DYKKQKQEEF 

      1090       1100       1110       1120       1130       1140 
KHIAVFPCKM KILPQYIFNS RDPIVIGVTV EAGQVKQGTP MCVPSKNFVD IGIVTSIEIN 

      1150       1160       1170       1180       1190       1200 
HKQVDVAKKG QEVCVKIEPI PGESPKMFGR HFEATDILVS KISRQSIDAL KDWFRDEMQK 

      1210 
SDWQLIVELK KVFEII 

« Hide

References

« Hide 'large scale' references
[1]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-771.
Strain: C57BL/6J.
Tissue: Corpora quadrigemina and Mammary gland.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-402.
Strain: FVB/N.
Tissue: Limb and Mammary tumor.
[4]"Exp5 exports eEF1A via tRNA from nuclei and synergizes with other transport pathways to confine translation to the cytoplasm."
Bohnsack M.T., Regener K., Schwappach B., Saffrich R., Paraskeva E., Hartmann E., Goerlich D.
EMBO J. 21:6205-6215(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[5]"Phosphoproteomic analysis of the developing mouse brain."
Ballif B.A., Villen J., Beausoleil S.A., Schwartz D., Gygi S.P.
Mol. Cell. Proteomics 3:1093-1101(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-215, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic brain.
[6]"A differential phosphoproteomic analysis of retinoic acid-treated P19 cells."
Smith J.C., Duchesne M.A., Tozzi P., Ethier M., Figeys D.
J. Proteome Res. 6:3174-3186(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-187 AND SER-191, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Teratocarcinoma.
[7]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-114; SER-137; SER-139; SER-187; SER-191 AND SER-215, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
[8]"Specific phosphopeptide enrichment with immobilized titanium ion affinity chromatography adsorbent for phosphoproteome analysis."
Zhou H., Ye M., Dong J., Han G., Jiang X., Wu R., Zou H.
J. Proteome Res. 7:3957-3967(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-137 AND SER-139, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
[9]"The phagosomal proteome in interferon-gamma-activated macrophages."
Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-165 AND SER-215, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-114; SER-137; SER-139 AND SER-215, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic fibroblast.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC114583 Genomic DNA. No translation available.
AK145146 mRNA. Translation: BAE26259.1.
AK145732 mRNA. Translation: BAE26614.1.
AK163527 mRNA. Translation: BAE37384.1.
BC018347 mRNA. Translation: AAH18347.1. Sequence problems.
BC037150 mRNA. Translation: AAH37150.1. Sequence problems.
BC040746 mRNA. Translation: AAH40746.1. Sequence problems.
BC060288 mRNA. Translation: AAH60288.1. Sequence problems.
CCDSCCDS35544.1.
RefSeqNP_938045.2. NM_198303.2.
UniGeneMm.260943.

3D structure databases

ProteinModelPortalQ05D44.
SMRQ05D44. Positions 583-1195.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid230580. 1 interaction.

PTM databases

PhosphoSiteQ05D44.

Proteomic databases

MaxQBQ05D44.
PaxDbQ05D44.
PRIDEQ05D44.

Protocols and materials databases

DNASU226982.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000027252; ENSMUSP00000027252; ENSMUSG00000026083.
GeneID226982.
KEGGmmu:226982.
UCSCuc007aso.1. mouse.

Organism-specific databases

CTD9669.
MGIMGI:2441772. Eif5b.

Phylogenomic databases

eggNOGCOG0532.
GeneTreeENSGT00730000111064.
HOVERGENHBG019036.
InParanoidQ8CFF4.
KOK03243.
OMAGSLRMHT.
OrthoDBEOG7034GC.
PhylomeDBQ05D44.
TreeFamTF101535.

Gene expression databases

ArrayExpressQ05D44.
BgeeQ05D44.
GenevestigatorQ05D44.

Family and domain databases

Gene3D3.40.50.10050. 1 hit.
3.40.50.300. 1 hit.
InterProIPR000795. EF_GTP-bd_dom.
IPR029459. EFTU-type.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR023115. TIF_IF2_dom3.
IPR009000. Transl_B-barrel.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view]
PfamPF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF14578. GTP_EFTU_D4. 1 hit.
PF11987. IF-2. 1 hit.
[Graphical view]
PRINTSPR00315. ELONGATNFCT.
SUPFAMSSF50447. SSF50447. 1 hit.
SSF52156. SSF52156. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00231. small_GTP. 1 hit.
ProtoNetSearch...

Other

ChiTaRSEIF5B. mouse.
NextBio378430.
PROQ05D44.
SOURCESearch...

Entry information

Entry nameIF2P_MOUSE
AccessionPrimary (citable) accession number: Q05D44
Secondary accession number(s): Q3SYI4 expand/collapse secondary AC list , Q3TQJ8, Q3UL37, Q3UM39, Q6PAI0, Q8CFF4, Q8CGD6
Entry history
Integrated into UniProtKB/Swiss-Prot: November 25, 2008
Last sequence update: November 25, 2008
Last modified: July 9, 2014
This is version 63 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

Translation initiation factors

List of translation initiation factor entries