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Protein

La-related protein 7

Gene

Larp7

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Negative transcriptional regulator of polymerase II genes, acting by means of the 7SK RNP system. Within the 7SK RNP complex, the positive transcription elongation factor b (P-TEFb) is sequestered in an inactive form, preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • germ cell proliferation Source: MGI
  • negative regulation of chromatin binding Source: MGI
  • negative regulation of transcription from RNA polymerase II promoter Source: MGI
  • positive regulation of G1/S transition of mitotic cell cycle Source: MGI
  • RNA processing Source: InterPro
Complete GO annotation...

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
La-related protein 7
Alternative name(s):
La ribonucleoprotein domain family member 7
Gene namesi
Name:Larp7
Synonyms:D3Wsu161e
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:107634. Larp7.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 570570La-related protein 7PRO_0000281144Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei251 – 2511PhosphothreonineCombined sources
Modified residuei253 – 2531PhosphoserineCombined sources
Modified residuei256 – 2561PhosphoserineCombined sources
Modified residuei260 – 2601PhosphothreonineCombined sources
Modified residuei334 – 3341PhosphoserineCombined sources
Modified residuei335 – 3351PhosphothreonineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ05CL8.
MaxQBiQ05CL8.
PaxDbiQ05CL8.
PRIDEiQ05CL8.

PTM databases

iPTMnetiQ05CL8.
PhosphoSiteiQ05CL8.

Expressioni

Developmental stagei

Ubiquitously expressed in the embryo.1 Publication

Gene expression databases

BgeeiQ05CL8.
CleanExiMM_LARP7.
ExpressionAtlasiQ05CL8. baseline and differential.
GenevisibleiQ05CL8. MM.

Interactioni

Subunit structurei

Integral part of the 7SK RNP complex. Specifically binds to the highly conserved 3'-terminal U-rich stretch of 7SK RNA. On stimulation, remains associated with 7SK RNA, whereas P-TEFb is released from the complex (By similarity).By similarity

Protein-protein interaction databases

IntActiQ05CL8. 4 interactions.
MINTiMINT-4613981.
STRINGi10090.ENSMUSP00000029588.

Structurei

3D structure databases

ProteinModelPortaliQ05CL8.
SMRiQ05CL8. Positions 23-214.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini22 – 11695HTH La-type RNA-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini119 – 19779RRMPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi196 – 364169Lys-richAdd
BLAST

Sequence similaritiesi

Contains 1 HTH La-type RNA-binding domain.PROSITE-ProRule annotation
Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IQXQ. Eukaryota.
COG5193. LUCA.
GeneTreeiENSGT00830000128323.
HOVERGENiHBG081891.
InParanoidiQ05CL8.
KOiK15191.
OMAiLSKTEWM.
OrthoDBiEOG72C50R.
PhylomeDBiQ05CL8.
TreeFamiTF314476.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
3.30.70.330. 2 hits.
InterProiIPR014886. La_RRM.
IPR002344. Lupus_La.
IPR006630. Lupus_La_RNA-bd.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF05383. La. 1 hit.
PF00076. RRM_1. 1 hit.
PF08777. RRM_3. 1 hit.
[Graphical view]
PRINTSiPR00302. LUPUSLA.
SMARTiSM00715. LA. 1 hit.
SM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF54928. SSF54928. 2 hits.
PROSITEiPS50961. HTH_LA. 1 hit.
PS50102. RRM. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q05CL8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
METENQKTME ESTKRKEEKK KRSRVKQVLA DIAKQVDFWF GDANLHKDKF
60 70 80 90 100
LREQIEKSRD GYVDISLLVS FNKMKKLTTD GKLIARALKS SSVVELDLEG
110 120 130 140 150
TRIRRKKPLG ERPKDEEERT VYVELLPKNV THSWIERVFG KCGNVVYISI
160 170 180 190 200
PHYKSTGDPK GFAFVEFETK EQAAKAIEFL NNPPEEAPRK PGIFPKTVKN
210 220 230 240 250
KPIPSLRVAE EKKKKKKKKG RIKKEESVQA KESAVDSSSS GVCKATKRPR
260 270 280 290 300
TASEGSEAET PEAPKQPAKK KKKRDKVEAS SLPEARAGKR ERCSAEDEDC
310 320 330 340 350
LPPRPKAKKR AQKDGVGQAA SEVSKESRDL EFCSTEEEKE TDRKGDSLSK
360 370 380 390 400
VKRKHKKKHK ERHKMGEEVI PLRVLSKTEW MDLKKEYLAL QKASMASLKK
410 420 430 440 450
TISQIKLESE METDCKAPTA GSGQECSTQE KVSAQGPQFV TGVIVKIVSG
460 470 480 490 500
EPLPGRKQVK DILATISEVV YIDLLEGDTE CHARFKTPED AQAVMNAQTE
510 520 530 540 550
IRKKHSWNLE VLSGDHEQRY WQKILVDRQA KLNQPREKKR GTEKLITKAE
560 570
KIRLAKTQQA SQHIRFSEYD
Length:570
Mass (Da):64,802
Last modified:March 20, 2007 - v2
Checksum:i025EB8EC3CA58F22
GO
Isoform 2 (identifier: Q05CL8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     179-185: FLNNPPE → VRTSVGP
     186-570: Missing.

Show »
Length:185
Mass (Da):21,334
Checksum:iA158BB9C4FA710EB
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti13 – 131T → TE in BC020127 (PubMed:15489334).Curated
Sequence conflicti202 – 2021P → L in AAH23165 (PubMed:15489334).Curated
Sequence conflicti214 – 2141K → R in BAB28459 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei179 – 1857FLNNPPE → VRTSVGP in isoform 2. 1 PublicationVSP_024022
Alternative sequencei186 – 570385Missing in isoform 2. 1 PublicationVSP_024023Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC113952 Genomic DNA. No translation available.
BC020127 mRNA. No translation available.
BC023165 mRNA. Translation: AAH23165.1. Different termination.
BC029178 mRNA. Translation: AAH29178.2.
AK012726 mRNA. Translation: BAB28435.1.
AK012772 mRNA. Translation: BAB28459.1.
AK015041 mRNA. Translation: BAB29687.1.
AK162147 mRNA. Translation: BAE36753.1.
CCDSiCCDS38626.1. [Q05CL8-1]
RefSeqiNP_613059.2. NM_138593.2. [Q05CL8-1]
UniGeneiMm.291032.

Genome annotation databases

EnsembliENSMUST00000029588; ENSMUSP00000029588; ENSMUSG00000027968. [Q05CL8-1]
GeneIDi28036.
KEGGimmu:28036.
UCSCiuc008rhd.1. mouse. [Q05CL8-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC113952 Genomic DNA. No translation available.
BC020127 mRNA. No translation available.
BC023165 mRNA. Translation: AAH23165.1. Different termination.
BC029178 mRNA. Translation: AAH29178.2.
AK012726 mRNA. Translation: BAB28435.1.
AK012772 mRNA. Translation: BAB28459.1.
AK015041 mRNA. Translation: BAB29687.1.
AK162147 mRNA. Translation: BAE36753.1.
CCDSiCCDS38626.1. [Q05CL8-1]
RefSeqiNP_613059.2. NM_138593.2. [Q05CL8-1]
UniGeneiMm.291032.

3D structure databases

ProteinModelPortaliQ05CL8.
SMRiQ05CL8. Positions 23-214.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ05CL8. 4 interactions.
MINTiMINT-4613981.
STRINGi10090.ENSMUSP00000029588.

PTM databases

iPTMnetiQ05CL8.
PhosphoSiteiQ05CL8.

Proteomic databases

EPDiQ05CL8.
MaxQBiQ05CL8.
PaxDbiQ05CL8.
PRIDEiQ05CL8.

Protocols and materials databases

DNASUi28036.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000029588; ENSMUSP00000029588; ENSMUSG00000027968. [Q05CL8-1]
GeneIDi28036.
KEGGimmu:28036.
UCSCiuc008rhd.1. mouse. [Q05CL8-1]

Organism-specific databases

CTDi51574.
MGIiMGI:107634. Larp7.

Phylogenomic databases

eggNOGiENOG410IQXQ. Eukaryota.
COG5193. LUCA.
GeneTreeiENSGT00830000128323.
HOVERGENiHBG081891.
InParanoidiQ05CL8.
KOiK15191.
OMAiLSKTEWM.
OrthoDBiEOG72C50R.
PhylomeDBiQ05CL8.
TreeFamiTF314476.

Miscellaneous databases

ChiTaRSiLarp7. mouse.
PROiQ05CL8.
SOURCEiSearch...

Gene expression databases

BgeeiQ05CL8.
CleanExiMM_LARP7.
ExpressionAtlasiQ05CL8. baseline and differential.
GenevisibleiQ05CL8. MM.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
3.30.70.330. 2 hits.
InterProiIPR014886. La_RRM.
IPR002344. Lupus_La.
IPR006630. Lupus_La_RNA-bd.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF05383. La. 1 hit.
PF00076. RRM_1. 1 hit.
PF08777. RRM_3. 1 hit.
[Graphical view]
PRINTSiPR00302. LUPUSLA.
SMARTiSM00715. LA. 1 hit.
SM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF54928. SSF54928. 2 hits.
PROSITEiPS50961. HTH_LA. 1 hit.
PS50102. RRM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-355 AND 388-570 (ISOFORM 1).
    Strain: Czech II and FVB/N.
    Tissue: Mammary tumor.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-342 AND 511-570 (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Egg, Embryo and Testis.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-260, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.
  6. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
    Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
    Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.
  7. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-251; SER-253; SER-256; THR-260; SER-334 AND THR-335, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Kidney, Lung, Pancreas, Spleen and Testis.
  8. "Loss of function mutation in LARP7, chaperone of 7SK ncRNA, causes a syndrome of facial dysmorphism, intellectual disability, and primordial dwarfism."
    Alazami A.M., Al-Owain M., Alzahrani F., Shuaib T., Al-Shamrani H., Al-Falki Y.H., Al-Qahtani S.M., Alsheddi T., Colak D., Alkuraya F.S.
    Hum. Mutat. 33:1429-1434(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiLARP7_MOUSE
AccessioniPrimary (citable) accession number: Q05CL8
Secondary accession number(s): Q3TSC2
, Q8K2Y6, Q8VDW3, Q9CSI2, Q9CSI9, Q9CUQ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: March 20, 2007
Last modified: June 8, 2016
This is version 88 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.