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Protein

Protein turtle homolog A

Gene

Igsf9

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions in dendrite outgrowth and synapse maturation.2 Publications

GO - Molecular functioni

  • protein binding involved in cell-cell adhesion Source: MGI

GO - Biological processi

  • dendrite development Source: MGI
  • homophilic cell adhesion via plasma membrane adhesion molecules Source: MGI
  • regulation of synapse organization Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Neurogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Protein turtle homolog A
Alternative name(s):
Dendrite arborization and synapse maturation protein 1
Immunoglobulin superfamily member 9A
Short name:
IgSF9A
Gene namesi
Name:Igsf9
Synonyms:Dasm1, Igsf9a, Nrt1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:2135283. Igsf9.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini21 – 734714ExtracellularSequence analysisAdd
BLAST
Transmembranei735 – 75521HelicalSequence analysisAdd
BLAST
Topological domaini756 – 1179424CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • axon Source: MGI
  • cell junction Source: UniProtKB-KW
  • dendrite Source: MGI
  • inhibitory synapse Source: MGI
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Synapse

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi1177 – 11771T → A: Loss of interaction with MAGI2 and SHANK1 and loss of function. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence analysisAdd
BLAST
Chaini21 – 11791159Protein turtle homolog APRO_0000306108Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi41 ↔ 108PROSITE-ProRule annotation
Disulfide bondi158 ↔ 206PROSITE-ProRule annotation
Glycosylationi188 – 1881N-linked (GlcNAc...)Sequence analysis
Glycosylationi239 – 2391N-linked (GlcNAc...)Sequence analysis
Disulfide bondi248 ↔ 301PROSITE-ProRule annotation
Glycosylationi256 – 2561N-linked (GlcNAc...)Sequence analysis
Disulfide bondi344 ↔ 395PROSITE-ProRule annotation
Disulfide bondi440 ↔ 486PROSITE-ProRule annotation
Glycosylationi513 – 5131N-linked (GlcNAc...)Sequence analysis
Glycosylationi524 – 5241N-linked (GlcNAc...)Sequence analysis
Modified residuei809 – 8091PhosphoserineBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ05BQ1.
PRIDEiQ05BQ1.

PTM databases

PhosphoSiteiQ05BQ1.

Expressioni

Tissue specificityi

Expressed in both cell bodies and dendrites of cortical and hippocampal neurons and also cerebellar Purkinje cells (at protein level).1 Publication

Developmental stagei

Detected in brain during embryonic development at least from E7.5 until E16.5. Mainly expressed within the dorsal root glanglia, trigeminal glanglia and olfactory epithelium of E10.5 embryos. Expressed to a lower extent in neuroepithelium, retina and hindgut.2 Publications

Gene expression databases

BgeeiQ05BQ1.
CleanExiMM_IGSF9.
ExpressionAtlasiQ05BQ1. baseline and differential.
GenevisibleiQ05BQ1. MM.

Interactioni

Subunit structurei

Interacts with MAGI2 and SHANK1.1 Publication

GO - Molecular functioni

  • protein binding involved in cell-cell adhesion Source: MGI

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000058275.

Structurei

3D structure databases

ProteinModelPortaliQ05BQ1.
SMRiQ05BQ1. Positions 626-718.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini24 – 124101Ig-like 1Add
BLAST
Domaini136 – 21681Ig-like 2Add
BLAST
Domaini226 – 31893Ig-like 3Add
BLAST
Domaini322 – 41089Ig-like 4Add
BLAST
Domaini418 – 50285Ig-like 5Add
BLAST
Domaini507 – 611105Fibronectin type-III 1PROSITE-ProRule annotationAdd
BLAST
Domaini623 – 71896Fibronectin type-III 2PROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi1177 – 11793PDZ-binding

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi823 – 974152Pro-richAdd
BLAST

Domaini

The PDZ-binding motif mediates interactions with MAGI2 and SHANK1.

Sequence similaritiesi

Contains 2 fibronectin type-III domains.PROSITE-ProRule annotation

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IQJK. Eukaryota.
ENOG410XNZS. LUCA.
GeneTreeiENSGT00530000063887.
HOGENOMiHOG000154716.
HOVERGENiHBG079460.
InParanoidiQ05BQ1.
OMAiRVEDQGW.
OrthoDBiEOG7M98FF.
PhylomeDBiQ05BQ1.
TreeFamiTF326128.

Family and domain databases

Gene3Di2.60.40.10. 7 hits.
InterProiIPR003961. FN3_dom.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
[Graphical view]
PfamiPF00041. fn3. 2 hits.
PF07679. I-set. 1 hit.
[Graphical view]
SMARTiSM00060. FN3. 2 hits.
SM00409. IG. 5 hits.
SM00408. IGc2. 4 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 5 hits.
SSF49265. SSF49265. 1 hit.
PROSITEiPS50853. FN3. 2 hits.
PS50835. IG_LIKE. 5 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q05BQ1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MIWCLRLTVL SLIISQGADG RRKPEVVSVV GRAGESAVLG CDLLPPAGHP
60 70 80 90 100
PLHVIEWLRF GFLLPIFIQF GLYSPRIDPD YVGRVRLQTG ASLQIEGLRV
110 120 130 140 150
EDQGWYECRV LFLDQHSPEQ DFANGSWVHL TVNSPPQFQE TPPLVLEVKE
160 170 180 190 200
LEAVTLRCVA RGSPQPYVTW KFRGQDLGKG QGQVQVQNGT LWIRRVERGS
210 220 230 240 250
AGDYTCQASS SEGSITHATQ LLVLGPPVIV VPPSNSTVNS SQDVSLACRA
260 270 280 290 300
EAYPANLTYS WFQDGVNVFH ISRLQSRVRI LVDGSLWLQA TQPDDAGHYT
310 320 330 340 350
CVPSNGFLHP PSASAYLTVL YPAQVTVMPP ETPLPTGMRG VIRCPVRANP
360 370 380 390 400
PLLFVTWTKD GQALQLDKFP GWSLGPEGSL IIALGNENAL GEYSCTPYNS
410 420 430 440 450
LGTAGPSPVT QVLLKAPPAF IDQPKEEYFQ EVGRELLIPC SARGDPPPIV
460 470 480 490 500
SWAKVGRGLQ GQAQVDSNNS LVLRPLTKEA QGRWECSASN AVARVTTSTN
510 520 530 540 550
VYVLGTSPHV VTNVSVVPLP KGANVSWEPG FDGGYLQRFS VWYTPLAKRP
560 570 580 590 600
DRAHHDWVSL AVPIGATHLL VPGLQAHAQY QFSVLAQNKL GSGPFSEIVL
610 620 630 640 650
SIPEGLPTTP AAPGLPPTEI PPPLSPPRGL VAVRTPRGVL LHWDPPELIP
660 670 680 690 700
GRLDGYILEG RQGSQGWEIL DQGVAGTEIQ LLVPGLIKDV LYEFRLVAFA
710 720 730 740 750
DSYVSDPSNV ANISTSGLEV YPSRTQLPGL LPQPVLAGVV GGVCFLGVAV
760 770 780 790 800
LVSILAACLM NRRRAARRHR KRLRQDPPLI FSPRGKSGSH SAPGSGSPDS
810 820 830 840 850
VTKFKLQGSP VPSLRQSLLW GEPARPPSPH PDSPLGRGPL PLEPICRGPD
860 870 880 890 900
GRFVMGPTVA PSQEKLCLER SEPRTSAKRL AQSFDCSSSS PSGVPQPLCI
910 920 930 940 950
TDISPVGQPL AAVPSPLPGP GPLLQYLSLP FFREMNVDGD WPPLEEPTPA
960 970 980 990 1000
PPPDFMDSQP CPTSSFLPPP DSPPANLRAV LPGTLMGVGV SSEPPYTALA
1010 1020 1030 1040 1050
DWTLRERVLP GLLSAAPRGS LTSQSSGRGS ASFLRPPSTA PSAGGSYLSP
1060 1070 1080 1090 1100
APGDTSSWAS GPERWPRREH VVTVSKRRNT SVDENYEWDS EFPGDMELLE
1110 1120 1130 1140 1150
TWHPGLASSR THPELEPELG VKTPEESCLL NPTHAAGPEA RCAALREEFL
1160 1170
AFRRRRDATR ARLPAYQQSI SYPEQATLL
Length:1,179
Mass (Da):127,330
Last modified:October 2, 2007 - v2
Checksum:i8CD76F4D1B32A2DF
GO
Isoform 2 (identifier: Q05BQ1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     505-528: GTSPHVVTNVSVVPLPKGANVSWE → EPSVLTEPTMTGYLWLCLSGLHTS
     529-1179: Missing.

Note: No experimental confirmation available.
Show »
Length:528
Mass (Da):57,608
Checksum:i5D74ADA7C166B951
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti49 – 491H → R in AAK49447 (PubMed:11401431).Curated
Sequence conflicti1161 – 11611A → V in AAH24487 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei505 – 52824GTSPH…NVSWE → EPSVLTEPTMTGYLWLCLSG LHTS in isoform 2. 1 PublicationVSP_028408Add
BLAST
Alternative sequencei529 – 1179651Missing in isoform 2. 1 PublicationVSP_028409Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC121551 Genomic DNA. No translation available.
BC024487 mRNA. Translation: AAH24487.1.
BC034594 mRNA. Translation: AAH34594.1.
AF284775 Genomic DNA. Translation: AAK49447.1.
CCDSiCCDS15515.1. [Q05BQ1-1]
RefSeqiNP_001139272.1. NM_001145800.1. [Q05BQ1-1]
NP_291086.2. NM_033608.3. [Q05BQ1-1]
XP_006497119.1. XM_006497056.2. [Q05BQ1-1]
UniGeneiMm.214530.

Genome annotation databases

EnsembliENSMUST00000052629; ENSMUSP00000058275; ENSMUSG00000037995. [Q05BQ1-1]
ENSMUST00000111235; ENSMUSP00000106866; ENSMUSG00000037995. [Q05BQ1-1]
ENSMUST00000127482; ENSMUSP00000117854; ENSMUSG00000037995. [Q05BQ1-2]
GeneIDi93842.
KEGGimmu:93842.
UCSCiuc007dqn.2. mouse. [Q05BQ1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC121551 Genomic DNA. No translation available.
BC024487 mRNA. Translation: AAH24487.1.
BC034594 mRNA. Translation: AAH34594.1.
AF284775 Genomic DNA. Translation: AAK49447.1.
CCDSiCCDS15515.1. [Q05BQ1-1]
RefSeqiNP_001139272.1. NM_001145800.1. [Q05BQ1-1]
NP_291086.2. NM_033608.3. [Q05BQ1-1]
XP_006497119.1. XM_006497056.2. [Q05BQ1-1]
UniGeneiMm.214530.

3D structure databases

ProteinModelPortaliQ05BQ1.
SMRiQ05BQ1. Positions 626-718.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000058275.

PTM databases

PhosphoSiteiQ05BQ1.

Proteomic databases

PaxDbiQ05BQ1.
PRIDEiQ05BQ1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000052629; ENSMUSP00000058275; ENSMUSG00000037995. [Q05BQ1-1]
ENSMUST00000111235; ENSMUSP00000106866; ENSMUSG00000037995. [Q05BQ1-1]
ENSMUST00000127482; ENSMUSP00000117854; ENSMUSG00000037995. [Q05BQ1-2]
GeneIDi93842.
KEGGimmu:93842.
UCSCiuc007dqn.2. mouse. [Q05BQ1-1]

Organism-specific databases

CTDi57549.
MGIiMGI:2135283. Igsf9.

Phylogenomic databases

eggNOGiENOG410IQJK. Eukaryota.
ENOG410XNZS. LUCA.
GeneTreeiENSGT00530000063887.
HOGENOMiHOG000154716.
HOVERGENiHBG079460.
InParanoidiQ05BQ1.
OMAiRVEDQGW.
OrthoDBiEOG7M98FF.
PhylomeDBiQ05BQ1.
TreeFamiTF326128.

Miscellaneous databases

ChiTaRSiIgsf9. mouse.
NextBioi351705.
PROiQ05BQ1.
SOURCEiSearch...

Gene expression databases

BgeeiQ05BQ1.
CleanExiMM_IGSF9.
ExpressionAtlasiQ05BQ1. baseline and differential.
GenevisibleiQ05BQ1. MM.

Family and domain databases

Gene3Di2.60.40.10. 7 hits.
InterProiIPR003961. FN3_dom.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
[Graphical view]
PfamiPF00041. fn3. 2 hits.
PF07679. I-set. 1 hit.
[Graphical view]
SMARTiSM00060. FN3. 2 hits.
SM00409. IG. 5 hits.
SM00408. IGc2. 4 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 5 hits.
SSF49265. SSF49265. 1 hit.
PROSITEiPS50853. FN3. 2 hits.
PS50835. IG_LIKE. 5 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of Igsf9 in mouse and human: a new member of the immunoglobulin superfamily expressed in the developing nervous system."
    Doudney K., Murdoch J.N., Braybrook C., Paternotte C., Bentley L., Copp A.J., Stanier P.
    Genomics 79:663-670(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), DEVELOPMENTAL STAGE.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 970-1179 (ISOFORM 1).
    Strain: FVB/N.
    Tissue: Colon and Eye.
  4. "Comparative physical and transcript maps of approximately 1 Mb around loop-tail, a gene for severe neural tube defects on distal mouse chromosome 1 and human chromosome 1q22-q23."
    Doudney K., Murdoch J.N., Paternotte C., Bentley L., Gregory S., Copp A.J., Stanier P.
    Genomics 72:180-192(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 21-83.
    Strain: 129S6/SvEvTac.
  5. "Control of dendrite arborization by an Ig family member, dendrite arborization and synapse maturation 1 (Dasm1)."
    Shi S.-H., Cox D.N., Wang D., Jan L.Y., Jan Y.-N.
    Proc. Natl. Acad. Sci. U.S.A. 101:13341-13345(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  6. "The immunoglobulin family member dendrite arborization and synapse maturation 1 (Dasm1) controls excitatory synapse maturation."
    Shi S.-H., Cheng T., Jan L.Y., Jan Y.-N.
    Proc. Natl. Acad. Sci. U.S.A. 101:13346-13351(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH MAGI2 AND SHANK1, MUTAGENESIS OF THR-1177.

Entry informationi

Entry nameiTUTLA_MOUSE
AccessioniPrimary (citable) accession number: Q05BQ1
Secondary accession number(s): Q8R1J8, Q925P4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: October 2, 2007
Last modified: May 11, 2016
This is version 76 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.