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Protein

Proton-coupled folate transporter

Gene

SLC46A1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Has been shown to act both as an intestinal proton-coupled high-affinity folate transporter and as an intestinal heme transporter which mediates heme uptake from the gut lumen into duodenal epithelial cells. The iron is then released from heme and may be transported into the bloodstream. Dietary heme iron is an important nutritional source of iron. Shows a higher affinity for folate than heme.By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transport

Keywords - Ligandi

Folate-binding

Enzyme and pathway databases

ReactomeiR-BTA-196757. Metabolism of folate and pterines.
R-BTA-917937. Iron uptake and transport.

Names & Taxonomyi

Protein namesi
Recommended name:
Proton-coupled folate transporter
Alternative name(s):
Heme carrier protein 1
PCFT/HCP1
Retinal pigment epithelium transporter
Solute carrier family 46 member 1
Gene namesi
Name:SLC46A1By similarity
Synonyms:HCP11 Publication, PCFTBy similarity, TRPEImported
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 19

Subcellular locationi

  • Apical cell membrane 1 Publication; Multi-pass membrane protein 1 Publication
  • Cytoplasm By similarity

  • Note: Localizes to the apical membrane of intestinal cells in iron-deficient cells, while it resides in internal cellular compartments in iron-replete cells.By similarity1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2424CytoplasmicSequence analysisAdd
BLAST
Transmembranei25 – 4824HelicalSequence analysisAdd
BLAST
Topological domaini49 – 8436ExtracellularSequence analysisAdd
BLAST
Transmembranei85 – 10723HelicalSequence analysisAdd
BLAST
Topological domaini108 – 1136CytoplasmicSequence analysis
Transmembranei114 – 13724HelicalSequence analysisAdd
BLAST
Topological domaini138 – 1458ExtracellularSequence analysis
Transmembranei146 – 16823HelicalSequence analysisAdd
BLAST
Topological domaini169 – 18012CytoplasmicSequence analysisAdd
BLAST
Transmembranei181 – 20323HelicalSequence analysisAdd
BLAST
Topological domaini204 – 2129ExtracellularSequence analysis
Transmembranei213 – 23624HelicalSequence analysisAdd
BLAST
Topological domaini237 – 26529CytoplasmicSequence analysisAdd
BLAST
Transmembranei266 – 28823HelicalSequence analysisAdd
BLAST
Topological domaini289 – 30921ExtracellularSequence analysisAdd
BLAST
Transmembranei310 – 32819HelicalSequence analysisAdd
BLAST
Topological domaini329 – 3313CytoplasmicSequence analysis
Transmembranei332 – 35625HelicalSequence analysisAdd
BLAST
Topological domaini357 – 3593ExtracellularSequence analysis
Transmembranei360 – 38122HelicalSequence analysisAdd
BLAST
Topological domaini382 – 39312CytoplasmicSequence analysisAdd
BLAST
Transmembranei394 – 41219HelicalSequence analysisAdd
BLAST
Topological domaini413 – 42412ExtracellularSequence analysisAdd
BLAST
Transmembranei425 – 44925HelicalSequence analysisAdd
BLAST
Topological domaini450 – 45910CytoplasmicSequence analysis

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 459459Proton-coupled folate transporterPRO_0000291649Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Glycosylationi58 – 581N-linked (GlcNAc...)Sequence analysis
Glycosylationi68 – 681N-linked (GlcNAc...)Sequence analysis
Modified residuei458 – 4581PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ05B81.

Expressioni

Tissue specificityi

Expressed in retina and retinal pigment epithelium.1 Publication

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000035161.

Structurei

3D structure databases

ProteinModelPortaliQ05B81.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2816. Eukaryota.
ENOG410ZVCA. LUCA.
GeneTreeiENSGT00530000063076.
HOGENOMiHOG000054191.
HOVERGENiHBG055334.
InParanoidiQ05B81.
KOiK14613.
OMAiTRHRENC.
OrthoDBiEOG7DNNVC.
TreeFamiTF315701.

Family and domain databases

InterProiIPR011701. MFS.
IPR020846. MFS_dom.
IPR005829. Sugar_transporter_CS.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q05B81-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEGRANSPGE PRAWPTRSVL CRGCVEPLVF LANFALVLQG PVTTQYLWHR
60 70 80 90 100
FSADLGYNGT RHRDSCSNHS VDPIAQEVET LTSHWTLYMN VGGFLVGLFS
110 120 130 140 150
STLLGAWSDC VGRRPLLVLA SLGLLLQTVL SIFVVQLHLH IGYLVLGRIL
160 170 180 190 200
CALLGDFSGL LAASFASVAD VSSSRTRTIR MALLEACIGV AGMLASFIGG
210 220 230 240 250
FLLQEQVYVN PFWLALAVLT VMTLYAAFCF GETVKERTPT RLFTLRHHRS
260 270 280 290 300
VIQLYVTQAP EKSRKHLALY SLAIFVMITV HLGAQDILTL YELSAPLCWD
310 320 330 340 350
SRLISYGSAA QQLPYLTSLL GLRLLQYCLA DTWVAEIGLV FNILGMMVFA
360 370 380 390 400
FATITPLMFT GYGLLFLSLV VTPIIRAKLS RLVRQSEQGA LFSALACVNG
410 420 430 440 450
LAMLMASGIF NSLYPATLNL MKGFPFLLAA GLLFIPAILM GILERDNHCP

EFQEFSQSP
Length:459
Mass (Da):50,428
Last modified:November 14, 2006 - v1
Checksum:i6468953BC5F14D85
GO

Sequence cautioni

The sequence ABM06094.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti2 – 21E → K in AAY45892 (PubMed:17335806).Curated
Sequence conflicti115 – 1151P → S in AAY45892 (PubMed:17335806).Curated
Sequence conflicti227 – 2271A → T in AAY45892 (PubMed:17335806).Curated
Sequence conflicti227 – 2271A → T in ABM06094 (PubMed:16305752).Curated
Sequence conflicti251 – 2511V → I in AAY45892 (PubMed:17335806).Curated
Sequence conflicti388 – 3881Q → H in AAY45892 (PubMed:17335806).Curated
Sequence conflicti429 – 4291A → G in AAY45892 (PubMed:17335806).Curated
Sequence conflicti429 – 4291A → G in ABM06094 (PubMed:16305752).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY999968 mRNA. Translation: AAY45892.1.
BC122640 mRNA. Translation: AAI22641.1.
BT029834 mRNA. Translation: ABM06094.1. Different initiation.
RefSeqiNP_001073053.1. NM_001079585.1.
UniGeneiBt.7353.

Genome annotation databases

EnsembliENSBTAT00000035283; ENSBTAP00000035161; ENSBTAG00000002817.
GeneIDi511097.
KEGGibta:511097.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY999968 mRNA. Translation: AAY45892.1.
BC122640 mRNA. Translation: AAI22641.1.
BT029834 mRNA. Translation: ABM06094.1. Different initiation.
RefSeqiNP_001073053.1. NM_001079585.1.
UniGeneiBt.7353.

3D structure databases

ProteinModelPortaliQ05B81.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000035161.

Proteomic databases

PaxDbiQ05B81.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000035283; ENSBTAP00000035161; ENSBTAG00000002817.
GeneIDi511097.
KEGGibta:511097.

Organism-specific databases

CTDi113235.

Phylogenomic databases

eggNOGiKOG2816. Eukaryota.
ENOG410ZVCA. LUCA.
GeneTreeiENSGT00530000063076.
HOGENOMiHOG000054191.
HOVERGENiHBG055334.
InParanoidiQ05B81.
KOiK14613.
OMAiTRHRENC.
OrthoDBiEOG7DNNVC.
TreeFamiTF315701.

Enzyme and pathway databases

ReactomeiR-BTA-196757. Metabolism of folate and pterines.
R-BTA-917937. Iron uptake and transport.

Miscellaneous databases

NextBioi20869775.

Family and domain databases

InterProiIPR011701. MFS.
IPR020846. MFS_dom.
IPR005829. Sugar_transporter_CS.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Heme carrier protein 1 (HCP1) expression and functional analysis in the retina and retinal pigment epithelium."
    Sharma S., Dimasi D., Broeer S., Kumar R., Della N.G.
    Exp. Cell Res. 313:1251-1259(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Tissue: Retinal pigment epithelium1 Publication.
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: HerefordImported.
    Tissue: Fetal skinImported.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 5-459.

Entry informationi

Entry nameiPCFT_BOVIN
AccessioniPrimary (citable) accession number: Q05B81
Secondary accession number(s): A1L0R5, A1L547
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 26, 2007
Last sequence update: November 14, 2006
Last modified: December 9, 2015
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.