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Protein

Proton-coupled folate transporter

Gene

SLC46A1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Has been shown to act both as an intestinal proton-coupled high-affinity folate transporter and as an intestinal heme transporter which mediates heme uptake from the gut lumen into duodenal epithelial cells. The iron is then released from heme and may be transported into the bloodstream. Dietary heme iron is an important nutritional source of iron. Shows a higher affinity for folate than heme.By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transport

Keywords - Ligandi

Folate-binding

Enzyme and pathway databases

ReactomeiR-BTA-196757. Metabolism of folate and pterines.
R-BTA-917937. Iron uptake and transport.

Names & Taxonomyi

Protein namesi
Recommended name:
Proton-coupled folate transporter
Alternative name(s):
Heme carrier protein 1
PCFT/HCP1
Retinal pigment epithelium transporter
Solute carrier family 46 member 1
Gene namesi
Name:SLC46A1By similarity
Synonyms:HCP11 Publication, PCFTBy similarity, TRPEImported
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 19

Subcellular locationi

  • Apical cell membrane 1 Publication; Multi-pass membrane protein 1 Publication
  • Cytoplasm By similarity

  • Note: Localizes to the apical membrane of intestinal cells in iron-deficient cells, while it resides in internal cellular compartments in iron-replete cells.By similarity1 Publication

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 24CytoplasmicSequence analysisAdd BLAST24
Transmembranei25 – 48HelicalSequence analysisAdd BLAST24
Topological domaini49 – 84ExtracellularSequence analysisAdd BLAST36
Transmembranei85 – 107HelicalSequence analysisAdd BLAST23
Topological domaini108 – 113CytoplasmicSequence analysis6
Transmembranei114 – 137HelicalSequence analysisAdd BLAST24
Topological domaini138 – 145ExtracellularSequence analysis8
Transmembranei146 – 168HelicalSequence analysisAdd BLAST23
Topological domaini169 – 180CytoplasmicSequence analysisAdd BLAST12
Transmembranei181 – 203HelicalSequence analysisAdd BLAST23
Topological domaini204 – 212ExtracellularSequence analysis9
Transmembranei213 – 236HelicalSequence analysisAdd BLAST24
Topological domaini237 – 265CytoplasmicSequence analysisAdd BLAST29
Transmembranei266 – 288HelicalSequence analysisAdd BLAST23
Topological domaini289 – 309ExtracellularSequence analysisAdd BLAST21
Transmembranei310 – 328HelicalSequence analysisAdd BLAST19
Topological domaini329 – 331CytoplasmicSequence analysis3
Transmembranei332 – 356HelicalSequence analysisAdd BLAST25
Topological domaini357 – 359ExtracellularSequence analysis3
Transmembranei360 – 381HelicalSequence analysisAdd BLAST22
Topological domaini382 – 393CytoplasmicSequence analysisAdd BLAST12
Transmembranei394 – 412HelicalSequence analysisAdd BLAST19
Topological domaini413 – 424ExtracellularSequence analysisAdd BLAST12
Transmembranei425 – 449HelicalSequence analysisAdd BLAST25
Topological domaini450 – 459CytoplasmicSequence analysis10

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002916491 – 459Proton-coupled folate transporterAdd BLAST459

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Glycosylationi58N-linked (GlcNAc...)Sequence analysis1
Glycosylationi68N-linked (GlcNAc...)Sequence analysis1
Modified residuei458PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ05B81.

Expressioni

Tissue specificityi

Expressed in retina and retinal pigment epithelium.1 Publication

Gene expression databases

BgeeiENSBTAG00000002817.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000035161.

Structurei

3D structure databases

ProteinModelPortaliQ05B81.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2816. Eukaryota.
ENOG410ZVCA. LUCA.
GeneTreeiENSGT00530000063076.
HOGENOMiHOG000054191.
HOVERGENiHBG055334.
InParanoidiQ05B81.
KOiK14613.
OMAiTRHRENC.
OrthoDBiEOG091G08MP.
TreeFamiTF315701.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR011701. MFS.
IPR020846. MFS_dom.
IPR005829. Sugar_transporter_CS.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q05B81-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEGRANSPGE PRAWPTRSVL CRGCVEPLVF LANFALVLQG PVTTQYLWHR
60 70 80 90 100
FSADLGYNGT RHRDSCSNHS VDPIAQEVET LTSHWTLYMN VGGFLVGLFS
110 120 130 140 150
STLLGAWSDC VGRRPLLVLA SLGLLLQTVL SIFVVQLHLH IGYLVLGRIL
160 170 180 190 200
CALLGDFSGL LAASFASVAD VSSSRTRTIR MALLEACIGV AGMLASFIGG
210 220 230 240 250
FLLQEQVYVN PFWLALAVLT VMTLYAAFCF GETVKERTPT RLFTLRHHRS
260 270 280 290 300
VIQLYVTQAP EKSRKHLALY SLAIFVMITV HLGAQDILTL YELSAPLCWD
310 320 330 340 350
SRLISYGSAA QQLPYLTSLL GLRLLQYCLA DTWVAEIGLV FNILGMMVFA
360 370 380 390 400
FATITPLMFT GYGLLFLSLV VTPIIRAKLS RLVRQSEQGA LFSALACVNG
410 420 430 440 450
LAMLMASGIF NSLYPATLNL MKGFPFLLAA GLLFIPAILM GILERDNHCP

EFQEFSQSP
Length:459
Mass (Da):50,428
Last modified:November 14, 2006 - v1
Checksum:i6468953BC5F14D85
GO

Sequence cautioni

The sequence ABM06094 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2E → K in AAY45892 (PubMed:17335806).Curated1
Sequence conflicti115P → S in AAY45892 (PubMed:17335806).Curated1
Sequence conflicti227A → T in AAY45892 (PubMed:17335806).Curated1
Sequence conflicti227A → T in ABM06094 (PubMed:16305752).Curated1
Sequence conflicti251V → I in AAY45892 (PubMed:17335806).Curated1
Sequence conflicti388Q → H in AAY45892 (PubMed:17335806).Curated1
Sequence conflicti429A → G in AAY45892 (PubMed:17335806).Curated1
Sequence conflicti429A → G in ABM06094 (PubMed:16305752).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY999968 mRNA. Translation: AAY45892.1.
BC122640 mRNA. Translation: AAI22641.1.
BT029834 mRNA. Translation: ABM06094.1. Different initiation.
RefSeqiNP_001073053.1. NM_001079585.1.
UniGeneiBt.7353.

Genome annotation databases

EnsembliENSBTAT00000035283; ENSBTAP00000035161; ENSBTAG00000002817.
GeneIDi511097.
KEGGibta:511097.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY999968 mRNA. Translation: AAY45892.1.
BC122640 mRNA. Translation: AAI22641.1.
BT029834 mRNA. Translation: ABM06094.1. Different initiation.
RefSeqiNP_001073053.1. NM_001079585.1.
UniGeneiBt.7353.

3D structure databases

ProteinModelPortaliQ05B81.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000035161.

Proteomic databases

PaxDbiQ05B81.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000035283; ENSBTAP00000035161; ENSBTAG00000002817.
GeneIDi511097.
KEGGibta:511097.

Organism-specific databases

CTDi113235.

Phylogenomic databases

eggNOGiKOG2816. Eukaryota.
ENOG410ZVCA. LUCA.
GeneTreeiENSGT00530000063076.
HOGENOMiHOG000054191.
HOVERGENiHBG055334.
InParanoidiQ05B81.
KOiK14613.
OMAiTRHRENC.
OrthoDBiEOG091G08MP.
TreeFamiTF315701.

Enzyme and pathway databases

ReactomeiR-BTA-196757. Metabolism of folate and pterines.
R-BTA-917937. Iron uptake and transport.

Gene expression databases

BgeeiENSBTAG00000002817.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR011701. MFS.
IPR020846. MFS_dom.
IPR005829. Sugar_transporter_CS.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPCFT_BOVIN
AccessioniPrimary (citable) accession number: Q05B81
Secondary accession number(s): A1L0R5, A1L547
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 26, 2007
Last sequence update: November 14, 2006
Last modified: September 7, 2016
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.