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Q05B57

- OTU1_BOVIN

UniProt

Q05B57 - OTU1_BOVIN

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Protein

Ubiquitin thioesterase OTU1

Gene

YOD1

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Hydrolase that can remove conjugated ubiquitin from proteins and participates in endoplasmic reticulum-associated degradation (ERAD) for misfolded lumenal proteins. May act by triming the ubiquitin chain on the associated substrate to facilitate their threading through the VCP/p97 pore. Ubiquitin moieties on substrates may present a steric impediment to the threading process when the substrate is transferred to the VCP pore and threaded through VCP's axial channel. Mediates deubiquitination of 'Lys-27'-, 'Lys-29'- and 'Lys-33'-linked polyubiquitin chains. Also able to hydrolyze 'Lys-11'-linked ubiquitin chains. Cleaves both polyubiquitin and di-ubiquitin (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei157 – 1571By similarity
Active sitei160 – 1601NucleophileBy similarity
Binding sitei266 – 2661Substrate; via carbonyl oxygenBy similarity
Active sitei267 – 2671By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri318 – 34225C2H2-typeAdd
BLAST

GO - Molecular functioni

  1. Lys48-specific deubiquitinase activity Source: Ensembl
  2. metal ion binding Source: UniProtKB-KW
  3. ubiquitin-specific protease activity Source: UniProtKB

GO - Biological processi

  1. cellular amino acid metabolic process Source: UniProtKB
  2. endoplasmic reticulum unfolded protein response Source: UniProtKB
  3. ER-associated ubiquitin-dependent protein catabolic process Source: UniProtKB
  4. protein K11-linked deubiquitination Source: UniProtKB
  5. protein K27-linked deubiquitination Source: UniProtKB
  6. protein K29-linked deubiquitination Source: UniProtKB
  7. protein K33-linked deubiquitination Source: UniProtKB
  8. protein K48-linked deubiquitination Source: UniProtKB
  9. protein K63-linked deubiquitination Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway, Unfolded protein response

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiC85.007.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin thioesterase OTU1 (EC:3.4.19.12)
Gene namesi
Name:YOD1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 16

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 348348Ubiquitin thioesterase OTU1PRO_0000282355Add
BLAST

Interactioni

Subunit structurei

Interacts with VCP; the interaction is direct. Interacts with FAF2/UBXD8. Interacts with DERL1; however interaction is dependent on the UBAX-like region, suggesting that it may be indirect (By similarity).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000027017.

Structurei

3D structure databases

ProteinModelPortaliQ05B57.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini149 – 274126OTUPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni50 – 12879UBX-likeAdd
BLAST
Regioni154 – 1607Cys-loopBy similarity
Regioni213 – 22311Variable-loopBy similarityAdd
BLAST
Regioni263 – 2675His-loopBy similarity
Regioni291 – 2966S2 siteBy similarity

Domaini

The UBAX-like region mediates the interaction with VCP.By similarity
The C2H2-type zinc finger mediates specificity for 'Lys-27'-, 'Lys-29'- and 'Lys-33'-linked polyubiquitin chains but not for 'Lys-11'-linked ubiquitin chains. Selectivity for 'Lys-11'-linked ubiquitin chains is provided by recognition of the sequence surrounding 'Lys-11' in ubiquitin. The S2 site region provides specificity for longer 'Lys-11'-linked ubiquitin chains (By similarity).By similarity

Sequence similaritiesi

Contains 1 C2H2-type zinc finger.Curated
Contains 1 OTU domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri318 – 34225C2H2-typeAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiCOG5539.
GeneTreeiENSGT00390000009989.
HOGENOMiHOG000193461.
HOVERGENiHBG097006.
InParanoidiQ05B57.
KOiK13719.
OMAiKSSRQFT.
OrthoDBiEOG7J1808.
TreeFamiTF323700.

Family and domain databases

InterProiIPR003323. OTU.
IPR029071. Ubiquitin-rel_dom.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
[Graphical view]
PfamiPF02338. OTU. 1 hit.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
PROSITEiPS50802. OTU. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q05B57-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFGPAKGGHF GVHPAAGCPG GVSQPAAGTK AGPAGVCPVG GRTNAMWRLR
60 70 80 90 100
CKAKEGTHVL QGLSSRTRVR ELQGQIAAIT GISPGCQRIL VGYPPECLDL
110 120 130 140 150
SNGDTILEDL PIQSGDMLIV EEDQNRPKTS PAFTKYGAPS YVRETLPVLA
160 170 180 190 200
RMAVPADNSC LFTSVYYVVE GGVLNPACAP EMRRFIAQIV ASDPDFYSEA
210 220 230 240 250
ILGKTNEEYC DWIKRDDTWG GAIEISILSK FYQCEICVVD TQTVRIDRFG
260 270 280 290 300
EDAGYTKRVL LIYDGIHYDP LHLVFPDPDT PPLTIFSSYD DIVLVQALEL
310 320 330 340
ADEARKKRQF TDVNRFTLRC MVCQKGLTGQ AEARDHAKET GHTNFGEV
Length:348
Mass (Da):38,021
Last modified:November 14, 2006 - v1
Checksum:i9CA87726C44F725F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC122788 mRNA. Translation: AAI22789.1.
RefSeqiNP_001073778.1. NM_001080309.2.
UniGeneiBt.52189.

Genome annotation databases

EnsembliENSBTAT00000027017; ENSBTAP00000027017; ENSBTAG00000020273.
GeneIDi539931.
KEGGibta:539931.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC122788 mRNA. Translation: AAI22789.1 .
RefSeqi NP_001073778.1. NM_001080309.2.
UniGenei Bt.52189.

3D structure databases

ProteinModelPortali Q05B57.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9913.ENSBTAP00000027017.

Protein family/group databases

MEROPSi C85.007.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSBTAT00000027017 ; ENSBTAP00000027017 ; ENSBTAG00000020273 .
GeneIDi 539931.
KEGGi bta:539931.

Organism-specific databases

CTDi 55432.

Phylogenomic databases

eggNOGi COG5539.
GeneTreei ENSGT00390000009989.
HOGENOMi HOG000193461.
HOVERGENi HBG097006.
InParanoidi Q05B57.
KOi K13719.
OMAi KSSRQFT.
OrthoDBi EOG7J1808.
TreeFami TF323700.

Miscellaneous databases

NextBioi 20878308.

Family and domain databases

InterProi IPR003323. OTU.
IPR029071. Ubiquitin-rel_dom.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
[Graphical view ]
Pfami PF02338. OTU. 1 hit.
[Graphical view ]
SMARTi SM00355. ZnF_C2H2. 1 hit.
[Graphical view ]
SUPFAMi SSF54236. SSF54236. 1 hit.
PROSITEi PS50802. OTU. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Crossbred X Angus.
    Tissue: Liver.

Entry informationi

Entry nameiOTU1_BOVIN
AccessioniPrimary (citable) accession number: Q05B57
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: November 14, 2006
Last modified: October 29, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3