Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q05B57 (OTU1_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin thioesterase OTU1

EC=3.4.19.12
Gene names
Name:YOD1
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length348 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Hydrolase that can remove conjugated ubiquitin from proteins and participates in endoplasmic reticulum-associated degradation (ERAD) for misfolded lumenal proteins. May act by triming the ubiquitin chain on the associated substrate to facilitate their threading through the VCP/p97 pore. Ubiquitin moieties on substrates may present a steric impediment to the threading process when the substrate is transferred to the VCP pore and threaded through VCP's axial channel. Mediates deubiquitination of 'Lys-27'-, 'Lys-29'- and 'Lys-33'-linked polyubiquitin chains. Also able to hydrolyze 'Lys-11'-linked ubiquitin chains. Cleaves both polyubiquitin and di-ubiquitin By similarity.

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Subunit structure

Interacts with VCP; the interaction is direct. Interacts with FAF2/UBXD8. Interacts with DERL1; however interaction is dependent on the UBAX-like region, suggesting that it may be indirect By similarity.

Domain

The UBAX-like region mediates the interaction with VCP By similarity.

The C2H2-type zinc finger mediates specificity for 'Lys-27'-, 'Lys-29'- and 'Lys-33'-linked polyubiquitin chains but not for 'Lys-11'-linked ubiquitin chains. Selectivity for 'Lys-11'-linked ubiquitin chains is provided by recognition of the sequence surrounding 'Lys-11' in ubiquitin. The S2 site region provides specificity for longer 'Lys-11'-linked ubiquitin chains By similarity.

Sequence similarities

Contains 1 C2H2-type zinc finger.

Contains 1 OTU domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 348348Ubiquitin thioesterase OTU1
PRO_0000282355

Regions

Domain149 – 274126OTU
Zinc finger318 – 34225C2H2-type
Region50 – 12879UBX-like
Region154 – 1607Cys-loop By similarity
Region213 – 22311Variable-loop By similarity
Region263 – 2675His-loop By similarity
Region291 – 2966S2 site By similarity

Sites

Active site1571 By similarity
Active site1601Nucleophile By similarity
Active site2671 By similarity
Binding site2661Substrate; via carbonyl oxygen By similarity

Sequences

Sequence LengthMass (Da)Tools
Q05B57 [UniParc].

Last modified November 14, 2006. Version 1.
Checksum: 9CA87726C44F725F

FASTA34838,021
        10         20         30         40         50         60 
MFGPAKGGHF GVHPAAGCPG GVSQPAAGTK AGPAGVCPVG GRTNAMWRLR CKAKEGTHVL 

        70         80         90        100        110        120 
QGLSSRTRVR ELQGQIAAIT GISPGCQRIL VGYPPECLDL SNGDTILEDL PIQSGDMLIV 

       130        140        150        160        170        180 
EEDQNRPKTS PAFTKYGAPS YVRETLPVLA RMAVPADNSC LFTSVYYVVE GGVLNPACAP 

       190        200        210        220        230        240 
EMRRFIAQIV ASDPDFYSEA ILGKTNEEYC DWIKRDDTWG GAIEISILSK FYQCEICVVD 

       250        260        270        280        290        300 
TQTVRIDRFG EDAGYTKRVL LIYDGIHYDP LHLVFPDPDT PPLTIFSSYD DIVLVQALEL 

       310        320        330        340 
ADEARKKRQF TDVNRFTLRC MVCQKGLTGQ AEARDHAKET GHTNFGEV 

« Hide

References

[1]NIH - Mammalian Gene Collection (MGC) project
Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Crossbred X Angus.
Tissue: Liver.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC122788 mRNA. Translation: AAI22789.1.
RefSeqNP_001073778.1. NM_001080309.2.
UniGeneBt.52189.

3D structure databases

ProteinModelPortalQ05B57.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9913.ENSBTAP00000027017.

Protein family/group databases

MEROPSC85.007.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSBTAT00000027017; ENSBTAP00000027017; ENSBTAG00000020273.
GeneID539931.
KEGGbta:539931.

Organism-specific databases

CTD55432.

Phylogenomic databases

eggNOGCOG5539.
GeneTreeENSGT00390000009989.
HOGENOMHOG000193461.
HOVERGENHBG097006.
InParanoidQ05B57.
KOK13719.
OMAFDGIHYD.
OrthoDBEOG7J1808.
TreeFamTF323700.

Family and domain databases

InterProIPR003323. OTU.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
[Graphical view]
PfamPF02338. OTU. 1 hit.
[Graphical view]
SMARTSM00355. ZnF_C2H2. 1 hit.
[Graphical view]
PROSITEPS50802. OTU. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20878308.

Entry information

Entry nameOTU1_BOVIN
AccessionPrimary (citable) accession number: Q05B57
Entry history
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: November 14, 2006
Last modified: February 19, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families