Q05B57 (OTU1_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin thioesterase OTU1
EC=3.4.19.12

Gene names

Name:

YOD1

Organism

Bos taurus (Bovine) [Reference proteome]

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	348 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Hydrolase that can remove conjugated ubiquitin from proteins and participates in endoplasmic reticulum-associated degradation (ERAD) for misfolded lumenal proteins. May act by triming the ubiquitin chain on the associated substrate to facilitate their threading through the VCP/p97 pore. Ubiquitin moieties on substrates may present a steric impediment to the threading process when the substrate is transferred to the VCP pore and threaded through VCP's axial channel. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Able to cleave both polyubiquitin and di-ubiquitin By similarity.
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Subunit structure	Interacts with VCP; the interaction is direct. Interacts with FAF2/UBXD8. Interacts with DERL1; however interaction is dependent on the UBAX-like region, suggesting that it may be indirect By similarity.
Domain	The UBAX-like region mediates the interaction with VCP By similarity.
Sequence similarities	Contains 1 C2H2-type zinc finger. Contains 1 OTU domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway Unfolded protein response
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Protease Thiol protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ER-associated protein catabolic process Inferred from sequence or structural similarity. Source: UniProtKB cellular amino acid metabolic process Inferred from sequence or structural similarity. Source: UniProtKB endoplasmic reticulum unfolded protein response Inferred from sequence or structural similarity. Source: UniProtKB protein K48-linked deubiquitination Inferred from sequence or structural similarity. Source: UniProtKB protein K63-linked deubiquitination Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular_function	ubiquitin-specific protease activity Inferred from sequence or structural similarity. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 348	348	Ubiquitin thioesterase OTU1		PRO_0000282355
Regions
	Domain	149 – 274	126	OTU
	Zinc finger	318 – 342	25	C2H2-type
	Region	50 – 128	79	UBX-like
Sites
	Active site	157	1	By similarity
	Active site	160	1	Nucleophile By similarity
	Active site	342	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q05B57 [UniParc]. Last modified November 14, 2006. Version 1. Checksum: 9CA87726C44F725F	FASTA	348	38,021
	10 20 30 40 50 60 MFGPAKGGHF GVHPAAGCPG GVSQPAAGTK AGPAGVCPVG GRTNAMWRLR CKAKEGTHVL 70 80 90 100 110 120 QGLSSRTRVR ELQGQIAAIT GISPGCQRIL VGYPPECLDL SNGDTILEDL PIQSGDMLIV 130 140 150 160 170 180 EEDQNRPKTS PAFTKYGAPS YVRETLPVLA RMAVPADNSC LFTSVYYVVE GGVLNPACAP 190 200 210 220 230 240 EMRRFIAQIV ASDPDFYSEA ILGKTNEEYC DWIKRDDTWG GAIEISILSK FYQCEICVVD 250 260 270 280 290 300 TQTVRIDRFG EDAGYTKRVL LIYDGIHYDP LHLVFPDPDT PPLTIFSSYD DIVLVQALEL 310 320 330 340 ADEARKKRQF TDVNRFTLRC MVCQKGLTGQ AEARDHAKET GHTNFGEV

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Crossbred X Angus. Tissue: Liver.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC122788 mRNA. Translation: AAI22789.1.
IPI	IPI00700582.
RefSeq	NP_001073778.1. NM_001080309.2.
UniGene	Bt.52189.
3D structure databases
ProteinModelPortal	Q05B57.
ModBase	Search...
Protein-protein interaction databases
STRING	9913.ENSBTAP00000027017.
Protein family/group databases
MEROPS	C85.007.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000027017; ENSBTAP00000027017; ENSBTAG00000020273.
GeneID	539931.
KEGG	bta:539931.
Organism-specific databases
CTD	55432.
Phylogenomic databases
eggNOG	COG5539.
GeneTree	ENSGT00390000009989.
HOGENOM	HOG000193461.
HOVERGEN	HBG097006.
InParanoid	Q05B57.
KO	K13719.
OMA	PIQSGDM.
OrthoDB	EOG4GF3FR.
Family and domain databases
InterPro	IPR003323. OTU. IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. [Graphical view]
Pfam	PF02338. OTU. 1 hit. [Graphical view]
SMART	SM00355. ZnF_C2H2. 1 hit. [Graphical view]
PROSITE	PS50802. OTU. 1 hit. PS00028. ZINC_FINGER_C2H2_1. 1 hit. PS50157. ZINC_FINGER_C2H2_2. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	20878308.

Entry information

Entry name

OTU1_BOVIN

Accession

Primary (citable) accession number: Q05B57

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2007
Last sequence update:	November 14, 2006
Last modified:	April 3, 2013
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program