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Q05B57

- OTU1_BOVIN

UniProt

Q05B57 - OTU1_BOVIN

Protein

Ubiquitin thioesterase OTU1

Gene

YOD1

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 55 (01 Oct 2014)
      Sequence version 1 (14 Nov 2006)
      Previous versions | rss
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    Functioni

    Hydrolase that can remove conjugated ubiquitin from proteins and participates in endoplasmic reticulum-associated degradation (ERAD) for misfolded lumenal proteins. May act by triming the ubiquitin chain on the associated substrate to facilitate their threading through the VCP/p97 pore. Ubiquitin moieties on substrates may present a steric impediment to the threading process when the substrate is transferred to the VCP pore and threaded through VCP's axial channel. Mediates deubiquitination of 'Lys-27'-, 'Lys-29'- and 'Lys-33'-linked polyubiquitin chains. Also able to hydrolyze 'Lys-11'-linked ubiquitin chains. Cleaves both polyubiquitin and di-ubiquitin By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei157 – 1571By similarity
    Active sitei160 – 1601NucleophileBy similarity
    Binding sitei266 – 2661Substrate; via carbonyl oxygenBy similarity
    Active sitei267 – 2671By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri318 – 34225C2H2-typeAdd
    BLAST

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. ubiquitin-specific protease activity Source: UniProtKB

    GO - Biological processi

    1. cellular amino acid metabolic process Source: UniProtKB
    2. endoplasmic reticulum unfolded protein response Source: UniProtKB
    3. ER-associated ubiquitin-dependent protein catabolic process Source: UniProtKB
    4. protein K11-linked deubiquitination Source: UniProtKB
    5. protein K27-linked deubiquitination Source: UniProtKB
    6. protein K29-linked deubiquitination Source: UniProtKB
    7. protein K33-linked deubiquitination Source: UniProtKB
    8. protein K48-linked deubiquitination Source: UniProtKB
    9. protein K63-linked deubiquitination Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Ubl conjugation pathway, Unfolded protein response

    Keywords - Ligandi

    Metal-binding, Zinc

    Protein family/group databases

    MEROPSiC85.007.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin thioesterase OTU1 (EC:3.4.19.12)
    Gene namesi
    Name:YOD1
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 16

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 348348Ubiquitin thioesterase OTU1PRO_0000282355Add
    BLAST

    Interactioni

    Subunit structurei

    Interacts with VCP; the interaction is direct. Interacts with FAF2/UBXD8. Interacts with DERL1; however interaction is dependent on the UBAX-like region, suggesting that it may be indirect By similarity.By similarity

    Protein-protein interaction databases

    STRINGi9913.ENSBTAP00000027017.

    Structurei

    3D structure databases

    ProteinModelPortaliQ05B57.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini149 – 274126OTUPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni50 – 12879UBX-likeAdd
    BLAST
    Regioni154 – 1607Cys-loopBy similarity
    Regioni213 – 22311Variable-loopBy similarityAdd
    BLAST
    Regioni263 – 2675His-loopBy similarity
    Regioni291 – 2966S2 siteBy similarity

    Domaini

    The UBAX-like region mediates the interaction with VCP.By similarity
    The C2H2-type zinc finger mediates specificity for 'Lys-27'-, 'Lys-29'- and 'Lys-33'-linked polyubiquitin chains but not for 'Lys-11'-linked ubiquitin chains. Selectivity for 'Lys-11'-linked ubiquitin chains is provided by recognition of the sequence surrounding 'Lys-11' in ubiquitin. The S2 site region provides specificity for longer 'Lys-11'-linked ubiquitin chains By similarity.By similarity

    Sequence similaritiesi

    Contains 1 C2H2-type zinc finger.Curated
    Contains 1 OTU domain.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri318 – 34225C2H2-typeAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5539.
    GeneTreeiENSGT00390000009989.
    HOGENOMiHOG000193461.
    HOVERGENiHBG097006.
    InParanoidiQ05B57.
    KOiK13719.
    OMAiKSSRQFT.
    OrthoDBiEOG7J1808.
    TreeFamiTF323700.

    Family and domain databases

    InterProiIPR003323. OTU.
    IPR029071. Ubiquitin-rel_dom.
    IPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    [Graphical view]
    PfamiPF02338. OTU. 1 hit.
    [Graphical view]
    SMARTiSM00355. ZnF_C2H2. 1 hit.
    [Graphical view]
    SUPFAMiSSF54236. SSF54236. 1 hit.
    PROSITEiPS50802. OTU. 1 hit.
    PS00028. ZINC_FINGER_C2H2_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q05B57-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFGPAKGGHF GVHPAAGCPG GVSQPAAGTK AGPAGVCPVG GRTNAMWRLR    50
    CKAKEGTHVL QGLSSRTRVR ELQGQIAAIT GISPGCQRIL VGYPPECLDL 100
    SNGDTILEDL PIQSGDMLIV EEDQNRPKTS PAFTKYGAPS YVRETLPVLA 150
    RMAVPADNSC LFTSVYYVVE GGVLNPACAP EMRRFIAQIV ASDPDFYSEA 200
    ILGKTNEEYC DWIKRDDTWG GAIEISILSK FYQCEICVVD TQTVRIDRFG 250
    EDAGYTKRVL LIYDGIHYDP LHLVFPDPDT PPLTIFSSYD DIVLVQALEL 300
    ADEARKKRQF TDVNRFTLRC MVCQKGLTGQ AEARDHAKET GHTNFGEV 348
    Length:348
    Mass (Da):38,021
    Last modified:November 14, 2006 - v1
    Checksum:i9CA87726C44F725F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC122788 mRNA. Translation: AAI22789.1.
    RefSeqiNP_001073778.1. NM_001080309.2.
    UniGeneiBt.52189.

    Genome annotation databases

    EnsembliENSBTAT00000027017; ENSBTAP00000027017; ENSBTAG00000020273.
    GeneIDi539931.
    KEGGibta:539931.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC122788 mRNA. Translation: AAI22789.1 .
    RefSeqi NP_001073778.1. NM_001080309.2.
    UniGenei Bt.52189.

    3D structure databases

    ProteinModelPortali Q05B57.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9913.ENSBTAP00000027017.

    Protein family/group databases

    MEROPSi C85.007.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000027017 ; ENSBTAP00000027017 ; ENSBTAG00000020273 .
    GeneIDi 539931.
    KEGGi bta:539931.

    Organism-specific databases

    CTDi 55432.

    Phylogenomic databases

    eggNOGi COG5539.
    GeneTreei ENSGT00390000009989.
    HOGENOMi HOG000193461.
    HOVERGENi HBG097006.
    InParanoidi Q05B57.
    KOi K13719.
    OMAi KSSRQFT.
    OrthoDBi EOG7J1808.
    TreeFami TF323700.

    Miscellaneous databases

    NextBioi 20878308.

    Family and domain databases

    InterProi IPR003323. OTU.
    IPR029071. Ubiquitin-rel_dom.
    IPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    [Graphical view ]
    Pfami PF02338. OTU. 1 hit.
    [Graphical view ]
    SMARTi SM00355. ZnF_C2H2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54236. SSF54236. 1 hit.
    PROSITEi PS50802. OTU. 1 hit.
    PS00028. ZINC_FINGER_C2H2_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. NIH - Mammalian Gene Collection (MGC) project
      Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Crossbred X Angus.
      Tissue: Liver.

    Entry informationi

    Entry nameiOTU1_BOVIN
    AccessioniPrimary (citable) accession number: Q05B57
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 3, 2007
    Last sequence update: November 14, 2006
    Last modified: October 1, 2014
    This is version 55 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3