Q05AS3 (SPAST_XENTR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Spastin
EC=3.6.4.3

Gene names

Name:	spast
Synonyms:	spg4
ORF Names:	TEgg045n18.1

Organism

Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)

Taxonomic identifier

8364 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Silurana

Protein attributes

Sequence length	603 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	ATP-dependent microtubule severing protein. Microtubule severing may promote reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. Required for membrane traffic from the endoplasmic reticulum (ER) to the Golgi and for completion of the abscission stage of cytokinesis. Also plays a role in axon growth and the formation of axonal branches By similarity.
Catalytic activity	ATP + H2O = ADP + phosphate.
Subunit structure	Homohexamer. The homohexamer is stabilized by ATP-binding. The homohexamer may adopt a ring conformation through which microtubules pass prior to being severed. Interacts with microtubules By similarity.
Subcellular location	Membrane; Single-pass membrane protein Potential. Cytoplasm › cytoskeleton › centrosome By similarity. Cytoplasm › cytoskeleton By similarity. Cytoplasm › perinuclear region By similarity. Nucleus By similarity.
Sequence similarities	Belongs to the AAA ATPase family. Spastin subfamily. Contains 1 MIT domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Differentiation Neurogenesis
Cellular component	Cytoplasm Cytoskeleton Membrane Microtubule Nucleus
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Molecular function	Developmental protein Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW microtubule bundle formation Inferred from sequence or structural similarity. Source: UniProtKB microtubule severing Inferred from sequence or structural similarity. Source: UniProtKB protein hexamerization Inferred from sequence or structural similarity. Source: UniProtKB protein homooligomerization Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW microtubule Inferred from electronic annotation. Source: UniProtKB-KW microtubule organizing center Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell perinuclear region of cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW alpha-tubulin binding Inferred from sequence or structural similarity. Source: UniProtKB beta-tubulin binding Inferred from sequence or structural similarity. Source: UniProtKB microtubule binding Inferred from sequence or structural similarity. Source: UniProtKB microtubule-severing ATPase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q05AS3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q05AS3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     189-220: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 603	603	Spastin		PRO_0000367139
Regions
	Transmembrane	54 – 76	23	Helical; Potential
	Domain	113 – 188	76	MIT
	Nucleotide binding	369 – 376	8	ATP Potential
Natural variations
	Alternative sequence	189 – 220	32	Missing in isoform 2.		VSP_036649
Experimental info
	Sequence conflict	9	1	N → D in CAJ82090. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 14, 2006. Version 1. Checksum: DF159AB3E69DAC00	FASTA	603	66,279
	10 20 30 40 50 60 MNSPGGRNNK KKPVTPAAET GPGPPTPPPP PAETQVLLAP PSLHKRNLYL FSYPLLAAFS 70 80 90 100 110 120 LLRFLAFQLG LLFVWFCERL SRRVMADKGS TAARTAAAPA QDRPQEPEVV RSYHQQAFQY 130 140 150 160 170 180 ISMALRIDEE EKDQKEQAIQ WYKKGIEELE KGIAVTITGK GEQYDRARRL QAKMSTNLLM 190 200 210 220 230 240 AKDRLQLLAK LKADIQGQHS QMEVCSDNTN LPCRNGLLKP EKGAVPKKKD PPSISSNSYS 250 260 270 280 290 300 RVKAAPKSGS LGNRIPNCTG VSSSARQAGP NAPSNRGAAG KNNTRTNKPT TPTTAVRKKD 310 320 330 340 350 360 MKNLRNVDSN LANLILNEIV DSGPTVKFAD IAGQDLAKQA LQEIVILPSI RPELFTGLRA 370 380 390 400 410 420 PARGLLLFGP PGNGKTMLAK AVAAESNATF FNISAASLTS KYVGEGEKLV RALFSVAREL 430 440 450 460 470 480 QPSIIFIDEV DSLLCERREG EHDASRRLKT EFLIEFDGVQ SGGDDRVLVM GATNRPQELD 490 500 510 520 530 540 DAVLRRFTKR VYVSLPNEET RLLLLKNLLS KQGNPLNEKE LTQLSRLTEG YSGSDITALA 550 560 570 580 590 600 KDAALGPIRE LKPEQVKNMA ASEMRNIKYS DFLSSLKKIK CSVSPSTLES YIRWNKEFGD TTV
	Isoform 2 [UniParc]. Checksum: 74E8F5C2B3603FB1	FASTA	571	62,787
	10 20 30 40 50 60 MNSPGGRNNK KKPVTPAAET GPGPPTPPPP PAETQVLLAP PSLHKRNLYL FSYPLLAAFS 70 80 90 100 110 120 LLRFLAFQLG LLFVWFCERL SRRVMADKGS TAARTAAAPA QDRPQEPEVV RSYHQQAFQY 130 140 150 160 170 180 ISMALRIDEE EKDQKEQAIQ WYKKGIEELE KGIAVTITGK GEQYDRARRL QAKMSTNLLM 190 200 210 220 230 240 AKDRLQLLEK GAVPKKKDPP SISSNSYSRV KAAPKSGSLG NRIPNCTGVS SSARQAGPNA 250 260 270 280 290 300 PSNRGAAGKN NTRTNKPTTP TTAVRKKDMK NLRNVDSNLA NLILNEIVDS GPTVKFADIA 310 320 330 340 350 360 GQDLAKQALQ EIVILPSIRP ELFTGLRAPA RGLLLFGPPG NGKTMLAKAV AAESNATFFN 370 380 390 400 410 420 ISAASLTSKY VGEGEKLVRA LFSVARELQP SIIFIDEVDS LLCERREGEH DASRRLKTEF 430 440 450 460 470 480 LIEFDGVQSG GDDRVLVMGA TNRPQELDDA VLRRFTKRVY VSLPNEETRL LLLKNLLSKQ 490 500 510 520 530 540 GNPLNEKELT QLSRLTEGYS GSDITALAKD AALGPIRELK PEQVKNMAAS EMRNIKYSDF 550 560 570 LSSLKKIKCS VSPSTLESYI RWNKEFGDTT V

References

[1]	Sanger Xenopus tropicalis EST/cDNA project Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Egg.
[2]	NIH - Xenopus Gene Collection (XGC) project Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: N6. Tissue: Skin.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR760974 mRNA. Translation: CAJ82090.1. BC123973 mRNA. Translation: AAI23974.1.
RefSeq	NP_001016453.1. NM_001016453.2.
UniGene	Str.10574.
3D structure databases
ProteinModelPortal	Q05AS3.
SMR	Q05AS3. Positions 311-595.
ModBase	Search...
Protein-protein interaction databases
STRING	Q05AS3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSXETT00000057121; ENSXETP00000057121; ENSXETG00000011392.
GeneID	549207.
KEGG	xtr:549207.
Organism-specific databases
CTD	6683.
Xenbase	XB-GENE-947839. spast.
Phylogenomic databases
GeneTree	ENSGT00570000078874.
HOVERGEN	HBG108502.
OMA	THKSTPK.
PhylomeDB	Q05AS3.
Gene expression databases
Bgee	Q05AS3.
Family and domain databases
InterPro	IPR003593. ATPase_AAA+_core. IPR003959. ATPase_AAA_core. IPR003960. ATPase_AAA_CS. IPR007330. MIT. IPR017179. Spastin. [Graphical view]
KO	K13254.
Pfam	PF00004. AAA. 1 hit. PF04212. MIT. 1 hit. [Graphical view]
PIRSF	PIRSF037338. Spastin. 1 hit.
SMART	SM00382. AAA. 1 hit. SM00745. MIT. 1 hit. [Graphical view]
PROSITE	PS00674. AAA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SPAST_XENTR

Accession

Primary (citable) accession number: Q05AS3
Secondary accession number(s): Q28H96

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	November 14, 2006
Last modified:	November 16, 2011
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program