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Protein

E3 ubiquitin-protein ligase DCST1

Gene

Dcst1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

E3 ubiquitin-protein ligase which mediates 'Lys-48'-linked ubiquitination of STAT2 and induces its proteosomal degradation thereby negatively regulating type-I-interferon signaling.By similarity

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.By similarity
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri672 – 711RING-type; degenerateAdd BLAST40

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processImmunity, Innate immunity, Ubl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase DCST1Curated (EC:2.3.2.27By similarity)
Alternative name(s):
DC-STAMP domain-containing protein 1
RING-type E3 ubiquitin transferaseCurated
Gene namesi
Name:Dcst1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1925022 Dcst1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 46CytoplasmicSequence analysisAdd BLAST46
Transmembranei47 – 67HelicalSequence analysisAdd BLAST21
Topological domaini68 – 76ExtracellularSequence analysis9
Transmembranei77 – 97HelicalSequence analysisAdd BLAST21
Topological domaini98 – 107CytoplasmicSequence analysis10
Transmembranei108 – 128HelicalSequence analysisAdd BLAST21
Topological domaini129 – 394ExtracellularSequence analysisAdd BLAST266
Transmembranei395 – 415HelicalSequence analysisAdd BLAST21
Topological domaini416 – 489CytoplasmicSequence analysisAdd BLAST74
Transmembranei490 – 510HelicalSequence analysisAdd BLAST21
Topological domaini511 – 576ExtracellularSequence analysisAdd BLAST66
Transmembranei577 – 597HelicalSequence analysisAdd BLAST21
Topological domaini598 – 732CytoplasmicSequence analysisAdd BLAST135

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002788251 – 732E3 ubiquitin-protein ligase DCST1Add BLAST732

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi184N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi217N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi346N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi374N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi551N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ059Y8
PRIDEiQ059Y8

PTM databases

iPTMnetiQ059Y8
PhosphoSitePlusiQ059Y8

Expressioni

Gene expression databases

BgeeiENSMUSG00000042672
CleanExiMM_DCST1
GenevisibleiQ059Y8 MM

Interactioni

Subunit structurei

Interacts with STAT2; the interaction results in STAT2 'Lys-48'-linked ubiquitination leading to its proteasomal degradation.By similarity

Family & Domainsi

Domaini

The RING-type zinc finger domain is responsible for E3 ubiquitin ligase activity.By similarity

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri672 – 711RING-type; degenerateAdd BLAST40

Keywords - Domaini

Transmembrane, Transmembrane helix, Zinc-finger

Phylogenomic databases

eggNOGiKOG3726 Eukaryota
ENOG410ZWQR LUCA
GeneTreeiENSGT00730000110972
HOGENOMiHOG000112180
HOVERGENiHBG081422
InParanoidiQ059Y8
KOiK22375
OMAiIYISTYF
OrthoDBiEOG091G0GX3
TreeFamiTF318254

Family and domain databases

InterProiView protein in InterPro
IPR012858 DC_STAMP-like
PfamiView protein in Pfam
PF07782 DC_STAMP, 1 hit

Sequencei

Sequence statusi: Complete.

Q059Y8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFLSSTLHS LGIFEKISRI KEVLKNRLLD LTKRRDQARE QQRKRPHTII
60 70 80 90 100
QGLLLWSLPV SWIRFLWRQP GEFPVTAFLL GAGTGGLLAI GLFQLLVNPM
110 120 130 140 150
NIYEEQKVVA LYCLASLGAI GWGTSPHIRC ASLLLVPKML GKEGRLFVMG
160 170 180 190 200
YALAAIYSGP AANLRSNINE VIASLGCTVE LQINNTRSAW RVSTAPLRAV
210 220 230 240 250
FKGMVGSKDS LNKEIQNVST SFEEMDEQVK SDAGYSSEDW DKNRESTEMF
260 270 280 290 300
GTSRVRPYLS TQMVYELRTR LRCIHVVNKA ILSCYRWFDK KHKNCMRRIH
310 320 330 340 350
LPLFNNMICV PMKFKFLCNI AKVIEIWCYK RIPVEGNFGQ TYDSVNQSIH
360 370 380 390 400
GLSGEFSANI NLKEEKQSSM VGLNTTNWEH MGTEVRDYVR QQETYLQWAM
410 420 430 440 450
GLLHVLLSCT FLLVFHSAFS YMDHYNWDIR FDNIYISTYF CQIDARRKKL
460 470 480 490 500
GKQSLLPLRK AERKTVIFPF KATIQAWEMR YVIRELLETL PIVLLLLVLC
510 520 530 540 550
AIDWALYSVF DTIRQHSFVQ YSFRSSHKLE IKVEGDSILA KLLRKTIGAL
560 570 580 590 600
NTSSSTDVET NNMPCLPQPI SLNARDYFKA SLPTLLLVCL CLAQAFGYRL
610 620 630 640 650
RRVIAAFYFP KREKKRALFF YNEFLKKRSA FTKLRRAAIV RRANQQKAPP
660 670 680 690 700
HYLVEALYRR CPLLHRFMRQ RCVVCQAMET PDSYVCPTPD CKALYCRSCW
710 720 730
DDMQRLCPVC TPREELSSSA HSDSNDDAVY GD
Length:732
Mass (Da):84,332
Last modified:November 14, 2006 - v1
Checksum:i22AB2CD98AC17CD9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC125478 mRNA Translation: AAI25479.1
BC125480 mRNA Translation: AAI25481.1
CCDSiCCDS38490.1
RefSeqiNP_084250.1, NM_029974.2
UniGeneiMm.164013

Genome annotation databases

EnsembliENSMUST00000070820; ENSMUSP00000065502; ENSMUSG00000042672
GeneIDi77772
KEGGimmu:77772
UCSCiuc008pyy.1 mouse

Similar proteinsi

Entry informationi

Entry nameiDCST1_MOUSE
AccessioniPrimary (citable) accession number: Q059Y8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 6, 2007
Last sequence update: November 14, 2006
Last modified: April 25, 2018
This is version 72 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health