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Protein

Thiamine transporter

Gene

THI7

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Responsible for intake of thiamine.

GO - Molecular functioni

  • 5-amino-1-ribofuranosylimidazole-4-carboxamide transporter activity Source: SGD
  • nucleobase transmembrane transporter activity Source: GO_Central
  • thiamine transmembrane transporter activity Source: SGD

GO - Biological processi

  • 5-amino-1-ribofuranosylimidazole-4-carboxamide transmembrane transport Source: SGD
  • nucleobase transport Source: GO_Central
  • thiamine transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-32346-MONOMER.

Protein family/group databases

TCDBi2.A.39.4.1. the nucleobase:cation symporter-1 (ncs1) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Thiamine transporter
Gene namesi
Name:THI7
Synonyms:THI10
Ordered Locus Names:YLR237W
ORF Names:L8083.2
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR237W.
SGDiS000004227. THI7.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4141CytoplasmicSequence analysisAdd
BLAST
Transmembranei42 – 6221HelicalSequence analysisAdd
BLAST
Topological domaini63 – 7311ExtracellularSequence analysisAdd
BLAST
Transmembranei74 – 9421HelicalSequence analysisAdd
BLAST
Topological domaini95 – 11117CytoplasmicSequence analysisAdd
BLAST
Transmembranei112 – 13221HelicalSequence analysisAdd
BLAST
Topological domaini133 – 17341ExtracellularSequence analysisAdd
BLAST
Transmembranei174 – 19421HelicalSequence analysisAdd
BLAST
Topological domaini195 – 1973CytoplasmicSequence analysis
Transmembranei198 – 21821HelicalSequence analysisAdd
BLAST
Topological domaini219 – 24022ExtracellularSequence analysisAdd
BLAST
Transmembranei241 – 26121HelicalSequence analysisAdd
BLAST
Topological domaini262 – 27413CytoplasmicSequence analysisAdd
BLAST
Transmembranei275 – 29521HelicalSequence analysisAdd
BLAST
Topological domaini296 – 33237ExtracellularSequence analysisAdd
BLAST
Transmembranei333 – 35321HelicalSequence analysisAdd
BLAST
Topological domaini354 – 37118CytoplasmicSequence analysisAdd
BLAST
Transmembranei372 – 39221HelicalSequence analysisAdd
BLAST
Topological domaini393 – 3942ExtracellularSequence analysis
Transmembranei395 – 41521HelicalSequence analysisAdd
BLAST
Topological domaini416 – 44631CytoplasmicSequence analysisAdd
BLAST
Transmembranei447 – 46721HelicalSequence analysisAdd
BLAST
Topological domaini468 – 48316ExtracellularSequence analysisAdd
BLAST
Transmembranei484 – 50421HelicalSequence analysisAdd
BLAST
Topological domaini505 – 59894CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 598598Thiamine transporterPRO_0000197926Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei560 – 5601PhosphoserineCombined sources
Modified residuei572 – 5721PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ05998.

PTM databases

iPTMnetiQ05998.

Interactioni

Protein-protein interaction databases

BioGridi31504. 42 interactions.
DIPiDIP-4784N.
IntActiQ05998. 2 interactions.
MINTiMINT-573771.

Structurei

3D structure databases

ProteinModelPortaliQ05998.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00530000065049.
HOGENOMiHOG000066000.
InParanoidiQ05998.
KOiK03457.
OMAiWFGSVSP.
OrthoDBiEOG7C2R9F.

Family and domain databases

InterProiIPR012681. NCS1.
IPR001248. Pur-cyt_permease.
[Graphical view]
PfamiPF02133. Transp_cyt_pur. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00800. ncs1. 1 hit.

Sequencei

Sequence statusi: Complete.

Q05998-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFGSKVSRA LRFLEIPVKD RASVSFLKNP DLQPIKSANQ TWGFWSNFAY
60 70 80 90 100
WGVMSFSVGT WMSASSALGV GLSYPETIGT FIVGDVLTII FTLANSCPGY
110 120 130 140 150
DWKVGFTLAQ RFVFGIYGSA FGIIIRILMS IVNYGSNAWV GGLCINMILD
160 170 180 190 200
SWSHHYLHLP NTLSSKVAMT TKELIGFIIF HVLTAFCYLM KPYHMNYILI
210 220 230 240 250
WSCVATFFSM LGMVIYLAKQ AHGVGELFTS TKSTATGSTK AWAWVYMISY
260 270 280 290 300
WFGSVSPGST NQSDYSRFGS SNWAIWAGTI CALLIPTTLI PVFGVIGAST
310 320 330 340 350
CDKLYGEQYW MPMDIFNHWL TTNYSAGARA GAFFCGLSFV LSQMSYTISN
360 370 380 390 400
CGFASGMDLA GLLPKYVDIK RGALFAACVS WACLPWNFYN SSSTFLTVMS
410 420 430 440 450
SFGVVMTPII SVMICDNFLI RKRQYSITNA FILKGEYYFT KGVNWRAIVA
460 470 480 490 500
WVCGMTPGLP GIAWEVNNDY FHNTGIVNFF YGDSFFSFLI SFFVYWGLCL
510 520 530 540 550
LFPFKITVKH DDKDYYGAFT DEEARKKGMV PYSEISEEEI RAYTLGEGYT
560 570 580 590
TGHEYRPEGS DDEIPELVKT SSENTNEFEI VHHKNNEKQS STASEKAA
Length:598
Mass (Da):66,904
Last modified:November 1, 1996 - v1
Checksum:i5160ADCAFC68A39E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U19027 Genomic DNA. Translation: AAB67405.1.
D55634 Genomic DNA. Translation: BAA09504.1.
BK006945 Genomic DNA. Translation: DAA09551.1.
PIRiS51456.
RefSeqiNP_013338.1. NM_001182124.1.

Genome annotation databases

EnsemblFungiiYLR237W; YLR237W; YLR237W.
GeneIDi850938.
KEGGisce:YLR237W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U19027 Genomic DNA. Translation: AAB67405.1.
D55634 Genomic DNA. Translation: BAA09504.1.
BK006945 Genomic DNA. Translation: DAA09551.1.
PIRiS51456.
RefSeqiNP_013338.1. NM_001182124.1.

3D structure databases

ProteinModelPortaliQ05998.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31504. 42 interactions.
DIPiDIP-4784N.
IntActiQ05998. 2 interactions.
MINTiMINT-573771.

Protein family/group databases

TCDBi2.A.39.4.1. the nucleobase:cation symporter-1 (ncs1) family.

PTM databases

iPTMnetiQ05998.

Proteomic databases

MaxQBiQ05998.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR237W; YLR237W; YLR237W.
GeneIDi850938.
KEGGisce:YLR237W.

Organism-specific databases

EuPathDBiFungiDB:YLR237W.
SGDiS000004227. THI7.

Phylogenomic databases

GeneTreeiENSGT00530000065049.
HOGENOMiHOG000066000.
InParanoidiQ05998.
KOiK03457.
OMAiWFGSVSP.
OrthoDBiEOG7C2R9F.

Enzyme and pathway databases

BioCyciYEAST:G3O-32346-MONOMER.

Miscellaneous databases

PROiQ05998.

Family and domain databases

InterProiIPR012681. NCS1.
IPR001248. Pur-cyt_permease.
[Graphical view]
PfamiPF02133. Transp_cyt_pur. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00800. ncs1. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and characterization of a thiamin transport gene, THI10, from Saccharomyces cerevisiae."
    Enjo F., Nosaka K., Ogata M., Iwashima A., Nishimura H.
    J. Biol. Chem. 272:19165-19170(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: S288c / GRF88.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
    Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
    , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
    Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  5. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.
  6. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-560, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiTHI7_YEAST
AccessioniPrimary (citable) accession number: Q05998
Secondary accession number(s): D6VYN5, P87335
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: July 6, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3120 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.