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Protein

Zona pellucida sperm-binding protein 2

Gene

ZP2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

The mammalian zona pellucida, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP2 may act as a secondary sperm receptor.By similarity

GO - Molecular functioni

  • acrosin binding Source: UniProtKB
  • coreceptor activity Source: ProtInc

GO - Biological processi

  • binding of sperm to zona pellucida Source: UniProtKB
  • intracellular protein transport Source: UniProtKB
  • prevention of polyspermy Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Biological processi

Fertilization

Enzyme and pathway databases

BioCyciZFISH:ENSG00000103310-MONOMER.
ReactomeiR-HSA-1300644. Interaction With The Zona Pellucida.

Names & Taxonomyi

Protein namesi
Recommended name:
Zona pellucida sperm-binding protein 2
Alternative name(s):
Zona pellucida glycoprotein 2
Short name:
Zp-2
Zona pellucida protein A
Cleaved into the following chain:
Gene namesi
Name:ZP2
Synonyms:ZPA
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

HGNCiHGNC:13188. ZP2.

Subcellular locationi

Processed zona pellucida sperm-binding protein 2 :
  • Secretedextracellular spaceextracellular matrix By similarity

  • Note: The glycoproteinaceous translucent extracellular matrix that surrounds the mammalian oocyte is called zona pellucida.By similarity
  • Cell membrane By similarity; Single-pass type I membrane protein By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini39 – 716ExtracellularSequence analysisAdd BLAST678
Transmembranei717 – 736HelicalSequence analysisAdd BLAST20
Topological domaini737 – 745CytoplasmicSequence analysis9

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Extracellular matrix, Membrane, Secreted

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000103310.
PharmGKBiPA37756.

Polymorphism and mutation databases

BioMutaiZP2.
DMDMi466206.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 38Add BLAST38
ChainiPRO_000004168939 – 640Zona pellucida sperm-binding protein 2Add BLAST602
ChainiPRO_000030455939 – ?Processed zona pellucida sperm-binding protein 2
PropeptideiPRO_0000041690641 – 745Removed in mature formBy similarityAdd BLAST105

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi88 ↔ 106By similarity
Glycosylationi105N-linked (GlcNAc...)Sequence analysis1
Glycosylationi122N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi372 ↔ 465By similarity
Disulfide bondi403 ↔ 424By similarity
Glycosylationi462O-linked (GalNAc...)By similarity1
Disulfide bondi545 ↔ 615By similarity
Disulfide bondi566 ↔ 634By similarity
Disulfide bondi620 ↔ 630By similarity

Post-translational modificationi

Proteolytically cleaved before the transmembrane segment to yield the secreted ectodomain incorporated in the zona pellucida.By similarity
Proteolytically cleaved in the N-terminal part by the metalloendopeptidase ASTL exocytosed from cortical granules after fertilization, yielding a N-terminal peptide of about 30 kDa which remains covalently attached to the C-terminal peptide via disulfide bond(s). This cleavage may play an important role in the post-fertilization block to polyspermy. Additional proteolytically cleavage of the N-terminal peptide of 30 kDa occurs in one-cell and two-cell embryos.By similarity
N-glycosylated.By similarity
O-glycosylated; contains sulfate-substituted glycans.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei172 – 173Cleavage; by ASTLBy similarity2
Sitei640 – 641CleavageBy similarity2

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ05996.
PaxDbiQ05996.
PeptideAtlasiQ05996.
PRIDEiQ05996.

PTM databases

iPTMnetiQ05996.
PhosphoSitePlusiQ05996.

Expressioni

Gene expression databases

BgeeiENSG00000103310.
CleanExiHS_ZP2.
GenevisibleiQ05996. HS.

Organism-specific databases

HPAiHPA011296.

Interactioni

Subunit structurei

Can form homopolymers that assemble into long fibers (in vitro). Polymers of ZP2 and ZP3 organized into long filaments cross-linked by ZP1 homodimers.By similarity

GO - Molecular functioni

  • acrosin binding Source: UniProtKB

Protein-protein interaction databases

IntActiQ05996. 2 interactors.
MINTiMINT-7241855.
STRINGi9606.ENSP00000219593.

Structurei

3D structure databases

ProteinModelPortaliQ05996.
SMRiQ05996.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini371 – 637ZPPROSITE-ProRule annotationAdd BLAST267

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni469 – 491DisorderedBy similarityAdd BLAST23

Domaini

The ZP domain is involved in the polymerization of the ZP proteins to form the zona pellucida.By similarity

Sequence similaritiesi

Belongs to the ZP domain family. ZPA subfamily.Curated
Contains 1 ZP domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IHBM. Eukaryota.
ENOG4110GZY. LUCA.
GeneTreeiENSGT00530000063096.
HOGENOMiHOG000059563.
HOVERGENiHBG004379.
InParanoidiQ05996.
KOiK19927.
OMAiDDCWATS.
OrthoDBiEOG091G02TE.
PhylomeDBiQ05996.
TreeFamiTF332794.

Family and domain databases

InterProiIPR001507. ZP_dom.
IPR017977. ZP_dom_CS.
[Graphical view]
PfamiPF00100. Zona_pellucida. 1 hit.
[Graphical view]
PRINTSiPR00023. ZPELLUCIDA.
SMARTiSM00241. ZP. 1 hit.
[Graphical view]
PROSITEiPS00682. ZP_1. 1 hit.
PS51034. ZP_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q05996-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MACRQRGGSW SPSGWFNAGW STYRSISLFF ALVTSGNSID VSQLVNPAFP
60 70 80 90 100
GTVTCDEREI TVEFPSSPGT KKWHASVVDP LGLDMPNCTY ILDPEKLTLR
110 120 130 140 150
ATYDNCTRRV HGGHQMTIRV MNNSAALRHG AVMYQFFCPA MQVEETQGLS
160 170 180 190 200
ASTICQKDFM SFSLPRVFSG LADDSKGTKV QMGWSIEVGD GARAKTLTLP
210 220 230 240 250
EAMKEGFSLL IDNHRMTFHV PFNATGVTHY VQGNSHLYMV SLKLTFISPG
260 270 280 290 300
QKVIFSSQAI CAPDPVTCNA THMTLTIPEF PGKLKSVSFE NQNIDVSQLH
310 320 330 340 350
DNGIDLEATN GMKLHFSKTL LKTKLSEKCL LHQFYLASLK LTFLLRPETV
360 370 380 390 400
SMVIYPECLC ESPVSIVTGE LCTQDGFMDV EVYSYQTQPA LDLGTLRVGN
410 420 430 440 450
SSCQPVFEAQ SQGLVRFHIP LNGCGTRYKF EDDKVVYENE IHALWTDFPP
460 470 480 490 500
SKISRDSEFR MTVKCSYSRN DMLLNINVES LTPPVASVKL GPFTLILQSY
510 520 530 540 550
PDNSYQQPYG ENEYPLVRFL RQPIYMEVRV LNRDDPNIKL VLDDCWATST
560 570 580 590 600
MDPDSFPQWN VVVDGCAYDL DNYQTTFHPV GSSVTHPDHY QRFDMKAFAF
610 620 630 640 650
VSEAHVLSSL VYFHCSALIC NRLSPDSPLC SVTCPVSSRH RRATGATEAE
660 670 680 690 700
KMTVSLPGPI LLLSDDSSFR GVGSSDLKAS GSSGEKSRSE TGEEVGSRGA
710 720 730 740
MDTKGHKTAG DVGSKAVAAV AAFAGVVATL GFIYYLYEKR TVSNH
Length:745
Mass (Da):82,357
Last modified:February 1, 1994 - v1
Checksum:i2614DA79738F2CDD
GO
Isoform 2 (identifier: Q05996-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     460-468: Missing.

Note: No experimental confirmation available.
Show »
Length:736
Mass (Da):81,300
Checksum:i0FA63E0D52271077
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_02470536G → V.1 PublicationCorresponds to variant rs2075520dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_054508460 – 468Missing in isoform 2. 1 Publication9

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M90366 mRNA. Translation: AAA61335.1.
AK313003 mRNA. Translation: BAG35839.1.
AF001550 Genomic DNA. Translation: AAB67599.1.
CH471228 Genomic DNA. Translation: EAW66859.1.
BC096304 mRNA. Translation: AAH96304.1.
BC096305 mRNA. Translation: AAH96305.1.
BC096306 mRNA. Translation: AAH96306.1.
BC096307 mRNA. Translation: AAH96307.1.
CCDSiCCDS10596.1. [Q05996-1]
CCDS73843.1. [Q05996-2]
PIRiA48833.
RefSeqiNP_001277033.1. NM_001290104.1. [Q05996-2]
NP_003451.1. NM_003460.2. [Q05996-1]
UniGeneiHs.73982.

Genome annotation databases

EnsembliENST00000574002; ENSP00000460971; ENSG00000103310. [Q05996-1]
ENST00000574091; ENSP00000458991; ENSG00000103310. [Q05996-2]
GeneIDi7783.
KEGGihsa:7783.
UCSCiuc002dii.3. human. [Q05996-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M90366 mRNA. Translation: AAA61335.1.
AK313003 mRNA. Translation: BAG35839.1.
AF001550 Genomic DNA. Translation: AAB67599.1.
CH471228 Genomic DNA. Translation: EAW66859.1.
BC096304 mRNA. Translation: AAH96304.1.
BC096305 mRNA. Translation: AAH96305.1.
BC096306 mRNA. Translation: AAH96306.1.
BC096307 mRNA. Translation: AAH96307.1.
CCDSiCCDS10596.1. [Q05996-1]
CCDS73843.1. [Q05996-2]
PIRiA48833.
RefSeqiNP_001277033.1. NM_001290104.1. [Q05996-2]
NP_003451.1. NM_003460.2. [Q05996-1]
UniGeneiHs.73982.

3D structure databases

ProteinModelPortaliQ05996.
SMRiQ05996.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ05996. 2 interactors.
MINTiMINT-7241855.
STRINGi9606.ENSP00000219593.

PTM databases

iPTMnetiQ05996.
PhosphoSitePlusiQ05996.

Polymorphism and mutation databases

BioMutaiZP2.
DMDMi466206.

Proteomic databases

MaxQBiQ05996.
PaxDbiQ05996.
PeptideAtlasiQ05996.
PRIDEiQ05996.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000574002; ENSP00000460971; ENSG00000103310. [Q05996-1]
ENST00000574091; ENSP00000458991; ENSG00000103310. [Q05996-2]
GeneIDi7783.
KEGGihsa:7783.
UCSCiuc002dii.3. human. [Q05996-1]

Organism-specific databases

CTDi7783.
GeneCardsiZP2.
HGNCiHGNC:13188. ZP2.
HPAiHPA011296.
MIMi182888. gene.
neXtProtiNX_Q05996.
OpenTargetsiENSG00000103310.
PharmGKBiPA37756.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IHBM. Eukaryota.
ENOG4110GZY. LUCA.
GeneTreeiENSGT00530000063096.
HOGENOMiHOG000059563.
HOVERGENiHBG004379.
InParanoidiQ05996.
KOiK19927.
OMAiDDCWATS.
OrthoDBiEOG091G02TE.
PhylomeDBiQ05996.
TreeFamiTF332794.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000103310-MONOMER.
ReactomeiR-HSA-1300644. Interaction With The Zona Pellucida.

Miscellaneous databases

GeneWikiiZP2.
GenomeRNAii7783.
PROiQ05996.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000103310.
CleanExiHS_ZP2.
GenevisibleiQ05996. HS.

Family and domain databases

InterProiIPR001507. ZP_dom.
IPR017977. ZP_dom_CS.
[Graphical view]
PfamiPF00100. Zona_pellucida. 1 hit.
[Graphical view]
PRINTSiPR00023. ZPELLUCIDA.
SMARTiSM00241. ZP. 1 hit.
[Graphical view]
PROSITEiPS00682. ZP_1. 1 hit.
PS51034. ZP_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiZP2_HUMAN
AccessioniPrimary (citable) accession number: Q05996
Secondary accession number(s): B2R7J2
, Q4VAN9, Q4VAP0, Q4VAP1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: November 2, 2016
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.