Reviewed, UniProtKB/Swiss-Prot Q05979 (KYNU_YEAST)

Names and origin

Protein names

Recommended name:
    Kynureninase
    EC=3.7.1.3
Alternative name(s):
    L-kynurenine hydrolase

Gene names

Name:	BNA5
Ordered Locus Names:	YLR231C
ORF Names:	L8083.14

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	453 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the cleavage of L-kynurenine (L-Kyn) and L-3-hydroxykynurenine (L-3OHKyn) into anthranilic (AA) and 3-hydroxyanthranilic acids (3-OHAA), respectively.
Catalytic activity	L-kynurenine + H(2)O = anthranilate + L-alanine. L-3-hydroxykynurenine + H(2)O = 3-hydroxyanthranilate + L-alanine.
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Amino-acid degradation; L-kynurenine degradation; L-alanine and anthranilate from L-kynurenine: step 1/1. Cofactor biosynthesis; NAD(+) biosynthesis; pyridine-2,3-dicarboxylate from L-kynurenine: step 2/3.
Subcellular location	Cytoplasm. Nucleus.
Miscellaneous	Present with 3060 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the kynureninase family.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Cellular component	Cytoplasm Nucleus
Ligand	Pyridoxal phosphate
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	de novo NAD biosynthetic process from tryptophan Ref.2 Inferred from genetic interaction. Source: SGD tryptophan catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Ref.3 Inferred from electronic annotation. Source: InterPro nucleus Ref.3 Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	kynureninase activity Ref.2 Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	P43582	1	EBI-10016,EBI-22766
ESS1	P22696	1	EBI-10016,EBI-6679
PRP40	P33203	1	EBI-10016,EBI-701
RSP5	P39940	1	EBI-10016,EBI-16219
SSM4	P40318	1	EBI-10016,EBI-18208
URN1	Q06525	1	EBI-10016,EBI-35138

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 453	453	Kynureninase		PRO_0000218665
Sites
	Binding site	252	1	Pyridoxal phosphate (covalent) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q05979-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 95C84CBB4F6FCF9B	FASTA	453	51,032
	10 20 30 40 50 60 MEKALELDGE YPESLRDEFN IPTFKSMGLS SDDKPVTYLC GNSLGLMPKS TRNSINAELD 70 80 90 100 110 120 AWSDCAVESH FKHPEEARGK VPWVSIDLPI LPLLAPIVGA QENEVAVMNS LTANLNSLLI 130 140 150 160 170 180 TFYKPTEKRF KILFEKGSFP SDYYAFYNQC KIHGISEPEN VFIQIEPREG ETYIRTQDIL 190 200 210 220 230 240 DTIEVNQDEL ALVCLSGVQY YTGQYFDIGR ITSFAHQFPD ILVGWDLAHA VGNVPLQLHD 250 260 270 280 290 300 WGVDFACWCS YKYLNAGPGG IGGLFVHSKH TKPDPAKESL PRLAGWWGND PAKRFQMLEV 310 320 330 340 350 360 FEPIPGALGF RQSNPSVIDT VALRSSLELF AKFNGINEVR KRSLLLTNYM TELLEASKYY 370 380 390 400 410 420 KHPLRIEKLP CFFTILTPTS TDEEHGAQLS LYFDSDTGKE DIMPKVFQYL HDHGVIGDAR 430 440 450 RPNVIRLAPA PLYNTFSDVY IAVNALNEAM DKL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D. Nature 387:87-90(1997) [PubMed: 9169871] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Aerobic and anaerobic NAD+ metabolism in Saccharomyces cerevisiae." Panozzo C., Nawara M., Suski C., Kucharczyka R., Skoneczny M., Becam A.-M., Rytka J., Herbert C.J. FEBS Lett. 517:97-102(2002) [PubMed: 12062417] [Abstract] Cited for: FUNCTION, PATHWAY.
[3]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U19027 Genomic DNA. Translation: AAB67417.1.
PIR	S51453.
RefSeq	NP_013332.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:6518N.
IntAct	Q05979.
Proteomic databases
PeptideAtlas	Q05979.
Genome annotation databases
Ensembl	YLR231C. Saccharomyces cerevisiae. [Contig view]
GeneID	850933.
GenomeReviews	Gene locus YLR231C in contig Y13138_GR.
KEGG	sce:YLR231C.
NMPDR	fig|4932.3.peg.4345.
Organism-specific databases
CYGD	YLR231c.
SGD	S000004221. BNA5.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	Q05979.
Enzyme and pathway databases
BioCyc	MetaCyc:MON-8163.
Gene expression databases
GermOnline	YLR231C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR010111. Kynureninase. IPR015421. PyrdxlP-dep_Trfase_major_sub1. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
PANTHER	PTHR14084. Kynureninase. 1 hit.
TIGRFAMs	TIGR01814. kynureninase. 1 hit.
ProtoNet	Search...
Other Resources
LinkHub	Q05979.
NextBio	967372.

Entry information

Entry name

KYNU_YEAST

Accession

Primary (citable) accession number: Q05979

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 1, 1996
Last modified:	November 25, 2008
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)