Reviewed, UniProtKB/Swiss-Prot Q05972 (CH601_SYNY3)

Names and origin

Protein names

Recommended name:
    60 kDa chaperonin 1
Alternative name(s):
    Protein Cpn60 1
    groEL protein 1

Gene names

Name:	groL1
Synonyms:	cpn60-1, groEL-1, groEL1
Ordered Locus Names:	slr2076

Organism

Synechocystis sp. (strain PCC 6803) [Complete proteome] [HAMAP]

Taxonomic identifier

1148 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechocystis

Protein attributes

Sequence length	541 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions By similarity.
Subunit structure	Oligomer of 14 subunits composed of two stacked rings of 7 subunits By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00600
Induction	By stress conditions e.g. heat shock. HAMAP MF_00600
Sequence similarities	Belongs to the chaperonin (HSP60) family.

Ontologies

Keywords
Biological process	Stress response
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Chaperone
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	protein refolding Inferred from electronic annotation. Source: HAMAP response to stress Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP unfolded protein binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
trxA	P52231	1	EBI-862119,EBI-862916

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1 Ref.3 Ref.4
	Chain	2 – 541	540	60 kDa chaperonin 1 HAMAP MF_00600		PRO_0000063575
Experimental info
	Sequence conflict	12	1	R → T AA sequence Ref.4
	Sequence conflict	91	1	A → D in BAA02180. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q05972-1 [UniParc]. Last modified January 23, 2007. Version 4. Checksum: 37E158A939CBFCB8	FASTA	541	57,653
	10 20 30 40 50 60 MAKSIIYNDE ARRALERGMD ILAEAVAVTL GPKGRNVVLE KKFGSPQIIN DGITIAKEIE 70 80 90 100 110 120 LEDHVENTGV SLIRQAASKT NDVAGDGTTT ATVLAHAIVK EGLRNVAAGA NPISLKRGID 130 140 150 160 170 180 KATDFLVARI KEHAQPVGDS KAIAQVGAIS AGNDEEVGQM IANAMDKVGQ EGVISLEEGK 190 200 210 220 230 240 SMTTELEITE GMRFDKGYIS PYFVTDAERM EAVLEDPRIL ITDKKINLVQ DLVPILEQVA 250 260 270 280 290 300 RQGKPLLIIA EDIEKEALAT LVVNRLRGVL NVAAVKAPGF GDRRKQMLED IATLTGGQVI 310 320 330 340 350 360 SEDAGLKLES ATVDSLGSAR RINITKDNTT IVAEGNEAAV KSRCEQIRRQ IEETDSSYDK 370 380 390 400 410 420 EKLQERLAKL AGGVAVIKVG AATETEMKDR KLRLEDAINA TKAAVEEGIV PGGGTTLAHL 430 440 450 460 470 480 APQLEDWATG NLKDEELTGA LIVARALPAP LKRIAENAGQ NGAVISERVK EKEFNVGYNA 490 500 510 520 530 540 ASLEYVDMLA AGIVDPAKVT RSALQNAASI AGMVLTTECI VVDKPEKEKA PAGAPGGDFD Y

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A second groEL-like gene, organized in a groESL operon is present in the genome of Synechocystis sp. PCC 6803." Lehel C., Los D.A., Wada H., Gyorgyei J., Horvath I., Kovacs E., Murata N., Vigh L. J. Biol. Chem. 268:1799-1804(1993) [PubMed: 8093614] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-26.
[2]	"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed: 8905231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Towards a proteome project of cyanobacterium Synechocystis sp. strain PCC6803: linking 130 protein spots with their respective genes." Sazuka T., Ohara O. Electrophoresis 18:1252-1258(1997) [PubMed: 9298645] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-21.
[4]	"Heat shock protein synthesis of the cyanobacterium Synechocystis PCC 6803: purification of the GroEL-related chaperonin." Lehel C., Wada H., Kovacs E., Toroek Z., Gombos Z., Horvath I., Murata N., Vigh L. Plant Mol. Biol. 18:327-336(1992) [PubMed: 1346251] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-26.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D12677 Genomic DNA. Translation: BAA02180.1. BA000022 Genomic DNA. Translation: BAA17411.1.
PIR	B44425.
RefSeq	NP_440731.1.
3D structure databases
HSSP	HSSP built from PDB template 1GRL based on UniProtKB P06139.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q05972. 2 interactions.
Genome annotation databases
GeneID	954034.
GenomeReviews	Gene locus slr2076 in contig BA000022_GR.
KEGG	syn:slr2076.
NMPDR	fig|1148.1.peg.832.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q05972.
OMA	Q05972. ELETWAN.
Enzyme and pathway databases
BioCyc	SSP1148:SLR2076-MON.
Family and domain databases
HAMAP	MF_00600. [Tree]
InterPro	IPR018370. Chaperonin_Cpn60_CS. IPR001844. Chaprnin_Cpn60. IPR002423. Cpn60/TCP-1. [Graphical view]
PANTHER	PTHR11353. Cpn60/TCP-1. 1 hit.
Pfam	PF00118. Cpn60_TCP1. 1 hit. [Graphical view]
PRINTS	PR00298. CHAPERONIN60.
TIGRFAMs	TIGR02348. GroEL. 1 hit.
PROSITE	PS00296. CHAPERONINS_CPN60. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CH601_SYNY3

Accession

Primary (citable) accession number: Q05972
Secondary accession number(s): P73379

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 67 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)