Skip Header

 
Contribute Send feedback
Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot Q05967 (PGLR_TOBAC)

Last modified June 16, 2009. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein namesRecommended name:
    Polygalacturonase
      Short name=PG
    EC=3.2.1.15
Alternative name(s):
    Pectinase
Gene names
Name: PG1
OrganismNicotiana tabacum (Common tobacco)
Taxonomic identifier4097 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

Hide | Top

Protein attributes

Sequence length396 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level.

Hide | Top

General annotation (Comments)

Function

May function in depolymerizing pectin during pollen development, germination, and tube growth.

Catalytic activity

Random hydrolysis of (1->4)-alpha-D-galactosiduronic linkages in pectate and other galacturonans.

Subcellular location

Secreted. Secreted › cell wall.

Tissue specificity

Pollen.

Sequence similarities

Belongs to the glycosyl hydrolase 28 family.

Contains 5 PbH1 repeats.

Hide | Top

Ontologies

Keywords
   Biological processCell wall biogenesis/degradation
   Cellular componentCell wall
Secreted
   DomainRepeat
Signal
   Molecular functionGlycosidase
Hydrolase
   PTMGlycoprotein
Gene Ontology (GO)
   Biological processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

cell wall organization

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcell wall

Inferred from electronic annotation. Source: UniProtKB-SubCell

extracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionpolygalacturonase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Hide | Top

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 Potential
Chain23 – 396374Polygalacturonase
PRO_0000024813

Sites

Active site2131Proton donor By similarity
Active site2361 By similarity

Amino acid modifications

Glycosylation1431N-linked (GlcNAc...) Potential
Glycosylation1511N-linked (GlcNAc...) Potential
Glycosylation1741N-linked (GlcNAc...) Potential
Glycosylation1811N-linked (GlcNAc...) Potential
Glycosylation2031N-linked (GlcNAc...) Potential
Glycosylation2081N-linked (GlcNAc...) Potential
Glycosylation2591N-linked (GlcNAc...) Potential
Glycosylation2941N-linked (GlcNAc...) Potential

Natural variations

Natural variant1541I → V
Natural variant2581R → S
Natural variant2621F → V
Natural variant2861H → R
Natural variant3431R → C in clones G27W, G27X and G27Y.
Natural variant3811G → D in clone G27W.

Hide | Top

Sequences

Sequence LengthMass (Da)Tools
Q05967-1 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: B3A486FB281E492B

FASTA39642,469
        10         20         30         40         50         60 
MDLKFKVHFA LVLLFLAHFG ESQTGVFDIT KYGANSNADI SEALLNAFKE ACQSTSPSTI 

        70         80         90        100        110        120 
VIPKGTFTMN QVKLEGPCKS PLELQIQATL KAPSDPSQLK VGEWLTVNKL DQFTMSGGGI 

       130        140        150        160        170        180 
LDGQAAAAWE CKQSKKCNKL PNNLSFNSLT NSTIKDITTL DSKSFHVNVN QCKNLTFIRF 

       190        200        210        220        230        240 
NVSAPANSPN TDGIHVSRSS SVNITDSNFS TGDDCISVGD ETEQLYITRV TCGPGHGISV 

       250        260        270        280        290        300 
GSLGGNPDEK PVVGVFVRNC TFTNTDNGVR IKTWPASHPG VVNDVHFEDI IVQNVSNPVV 

       310        320        330        340        350        360 
IDQVYCPFNK CNKDLPSQVK ISKVSFQNIK GTSRTQDAVS LLRSKGVPCE GIEVGDIDIT 

       370        380        390 
YSGKEGPAKS SCENIKPSLK GKQNPPVCTA SAASSS

« Hide

Hide | Top

References

[1]"Characterization of a tobacco gene encoding a pollen-specific polygalacturonase."
Tebbutt S.J., Lonsdale D.M.
Plant Mol. Biol. 25:283-297(1994) [PubMed: 8018876] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
Strain: cv. Havana.
Tissue: Pollen.

Hide | Top

Cross-references

Sequence databases

X71020 Genomic DNA. Translation: CAA50338.1.
X71017 mRNA. Translation: CAA50335.1. Different initiation.
X71018 mRNA. Translation: CAA50336.1. Different initiation.
X71016 mRNA. Translation: CAA50334.1. Different initiation.
X71019 mRNA. Translation: CAA50337.1. Different initiation.
PIRS46532.
S46533.

3D structure databases

ModBaseSearch...

Protein family/group databases

CAZyGH28. Glycoside Hydrolase Family 28.

Enzyme and pathway databases

BRENDA3.2.1.15. 298.

Family and domain databases

InterProIPR000743. Glyco_hydro_28.
IPR006626. PbH1.
IPR012334. Pectin_lyas_fold.
[Graphical view]
Gene3DG3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
PfamPF00295. Glyco_hydro_28. 1 hit.
[Graphical view]
SMARTSM00710. PbH1. 5 hits.
[Graphical view]
PROSITEPS00502. POLYGALACTURONASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Hide | Top

Entry information

Entry namePGLR_TOBAC
AccessionPrimary (citable) accession number: Q05967
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: June 16, 2009
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

Hide | Top

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents