ID   FL3H_DIACA              Reviewed;         365 AA.
AC   Q05964;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   05-MAY-2009, entry version 47.
DE   RecName: Full=Naringenin,2-oxoglutarate 3-dioxygenase;
DE            EC=1.14.11.9;
DE   AltName: Full=Flavonone-3-hydroxylase;
DE            Short=F3H;
DE   AltName: Full=FHT;
GN   Name=FHT;
OS   Dianthus caryophyllus (Carnation) (Clove pink).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   Caryophyllales; Caryophyllaceae; Dianthus.
OX   NCBI_TaxID=3570;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Flower bud;
RX   MEDLINE=94039114; PubMed=8223617;
RX   DOI=10.1111/j.1432-1033.1993.tb18301.x;
RA   Britsch L., Dedio J., Saedler H., Forkmann G.;
RT   "Molecular characterization of flavanone 3 beta-hydroxylases.
RT   Consensus sequence, comparison with related enzymes and the role of
RT   conserved histidine residues.";
RL   Eur. J. Biochem. 217:745-754(1993).
CC   -!- FUNCTION: Catalyzes the 3-beta-hydroxylation of 2S-flavonones to
CC       2R,3R-dihydroflavonols which are intermediates in the biosynthesis
CC       of flavonols, anthocyanidins, catechins and proanthocyanidins in
CC       plants.
CC   -!- CATALYTIC ACTIVITY: A flavanone + 2-oxoglutarate + O(2) = a
CC       dihydroflavonol + succinate + CO(2).
CC   -!- COFACTOR: Iron.
CC   -!- COFACTOR: Ascorbate.
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid
CC       biosynthesis.
CC   -!- SIMILARITY: Belongs to the iron/ascorbate-dependent oxidoreductase
CC       family.
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DR   EMBL; X72592; CAA51190.1; -; mRNA.
DR   EMBL; X70378; CAA49839.1; -; Genomic_DNA.
DR   PIR; S31921; S31921.
DR   HSSP; Q96323; 1GP6.
DR   BRENDA; 1.14.11.9; 21500.
DR   GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0031418; F:L-ascorbic acid binding; IEA:UniProtKB-KW.
DR   GO; GO:0045486; F:naringenin 3-dioxygenase activity; IEA:EC.
DR   GO; GO:0016702; F:oxidoreductase activity, acting on single d...; IEA:UniProtKB-KW.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR005123; Oxoglutarate/Fe-dep_Oase.
DR   Pfam; PF03171; 2OG-FeII_Oxy; 1.
PE   2: Evidence at transcript level;
KW   Dioxygenase; Flavonoid biosynthesis; Iron; Metal-binding;
KW   Oxidoreductase; Vitamin C.
FT   CHAIN         1    365       Naringenin,2-oxoglutarate 3-dioxygenase.
FT                                /FTId=PRO_0000067285.
FT   METAL        76     76       Iron (Potential).
FT   METAL       218    218       Iron (Potential).
FT   METAL       220    220       Iron (Potential).
FT   METAL       276    276       Iron (Potential).
SQ   SEQUENCE   365 AA;  40964 MW;  55F39E674A9293C9 CRC64;
     MVAEKPKTLT SLEGDDKLNS NFVRDEDERP KVAYNEFSND IPVISLAGID GEKRGEICRK
     IVEACEDWGI FQVVDHGVGD DLIADMTRLA REFFALPAEE KLRFDMSGGK KGGFIVSSHL
     QGEVVQDWRE IVTYFSYPTN SRDYTRWPDK PEGWIKVTEE YSNKLMTLAC TLLGVLSEAM
     GLELEALTKA CVDMDQKIVV NYYPKCPQPD LTLGLKRHTD PGTITLLLQD QVGGLQATRD
     GGKTWITVQP VPGAFVVNLG DHGHFLSNGR FKNADHQAVV NSECSRLSIA TFQNPSPDAT
     VYPLAIREGE NSIMEEPITF ADLYRRKMAK DLEIARHKRL AKEEMPFKEL DEAKFESKSI
     DQILA
//