Reviewed, UniProtKB/Swiss-Prot Q05964 (FL3H_DIACA)

Names and origin

Protein names

Recommended name:
    Naringenin,2-oxoglutarate 3-dioxygenase
    EC=1.14.11.9
Alternative name(s):
    Flavanone-3-hydroxylase
      Short name=F3H
    FHT

Gene names

Name:

FHT

Organism

Dianthus caryophyllus (Carnation) (Clove pink)

Taxonomic identifier

3570 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › Caryophyllales › Caryophyllaceae › Dianthus

Protein attributes

Sequence length	365 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the 3-beta-hydroxylation of 2S-flavanones to 2R,3R-dihydroflavonols which are intermediates in the biosynthesis of flavonols, anthocyanidins, catechins and proanthocyanidins in plants.
Catalytic activity	A flavanone + 2-oxoglutarate + O2 = a dihydroflavonol + succinate + CO2.
Cofactor	Iron. Ascorbate.
Pathway	Secondary metabolite biosynthesis; flavonoid biosynthesis.
Sequence similarities	Belongs to the iron/ascorbate-dependent oxidoreductase family. Contains 1 Fe2OG dioxygenase domain.

Ontologies

Keywords
Biological process	Flavonoid biosynthesis
Ligand	Iron Metal-binding Vitamin C
Molecular function	Dioxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	flavonoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	L-ascorbic acid binding Inferred from electronic annotation. Source: UniProtKB-KW iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW naringenin 3-dioxygenase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 365	365	Naringenin,2-oxoglutarate 3-dioxygenase		PRO_0000067285
Regions
	Domain	191 – 295	105	Fe2OG dioxygenase
Sites
	Metal binding	76	1	Iron Potential
	Metal binding	218	1	Iron Potential
	Metal binding	220	1	Iron Potential
	Metal binding	276	1	Iron Potential

Sequences

	Sequence		Length	Mass (Da)
	Q05964-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 55F39E674A9293C9	FASTA	365	40,964
	10 20 30 40 50 60 MVAEKPKTLT SLEGDDKLNS NFVRDEDERP KVAYNEFSND IPVISLAGID GEKRGEICRK 70 80 90 100 110 120 IVEACEDWGI FQVVDHGVGD DLIADMTRLA REFFALPAEE KLRFDMSGGK KGGFIVSSHL 130 140 150 160 170 180 QGEVVQDWRE IVTYFSYPTN SRDYTRWPDK PEGWIKVTEE YSNKLMTLAC TLLGVLSEAM 190 200 210 220 230 240 GLELEALTKA CVDMDQKIVV NYYPKCPQPD LTLGLKRHTD PGTITLLLQD QVGGLQATRD 250 260 270 280 290 300 GGKTWITVQP VPGAFVVNLG DHGHFLSNGR FKNADHQAVV NSECSRLSIA TFQNPSPDAT 310 320 330 340 350 360 VYPLAIREGE NSIMEEPITF ADLYRRKMAK DLEIARHKRL AKEEMPFKEL DEAKFESKSI DQILA

References

[1]

"Molecular characterization of flavanone 3 beta-hydroxylases. Consensus sequence, comparison with related enzymes and the role of conserved histidine residues." Britsch L., Dedio J., Saedler H., Forkmann G. Eur. J. Biochem. 217:745-754(1993) [PubMed: 8223617] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Flower bud.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X72592 mRNA. Translation: CAA51190.1. X70378 Genomic DNA. Translation: CAA49839.1.
PIR	S31921.
3D structure databases
SMR	Q05964. Positions 13-330.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.14.11.9. 21500.
Family and domain databases
InterPro	IPR005123. Oxoglutarate/Fe-dep_oxygenase. [Graphical view]
Pfam	PF03171. 2OG-FeII_Oxy. 1 hit. [Graphical view]
PROSITE	PS51471. FE2OG_OXY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FL3H_DIACA

Accession

Primary (citable) accession number: Q05964

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	January 19, 2010
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)