ID   FL3H_CALCH              Reviewed;         356 AA.
AC   Q05963;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   05-MAY-2009, entry version 49.
DE   RecName: Full=Naringenin,2-oxoglutarate 3-dioxygenase;
DE            EC=1.14.11.9;
DE   AltName: Full=Flavonone-3-hydroxylase;
DE            Short=F3H;
DE   AltName: Full=FHT;
GN   Name=FHT;
OS   Callistephus chinensis (China aster).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   asterids; campanulids; Asterales; Asteraceae; Asteroideae; Astereae;
OC   Callistephus.
OX   NCBI_TaxID=13379;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Flower bud;
RX   MEDLINE=94039114; PubMed=8223617;
RX   DOI=10.1111/j.1432-1033.1993.tb18301.x;
RA   Britsch L., Dedio J., Saedler H., Forkmann G.;
RT   "Molecular characterization of flavanone 3 beta-hydroxylases.
RT   Consensus sequence, comparison with related enzymes and the role of
RT   conserved histidine residues.";
RL   Eur. J. Biochem. 217:745-754(1993).
CC   -!- FUNCTION: Catalyzes the 3-beta-hydroxylation of 2S-flavonones to
CC       2R,3R-dihydroflavonols which are intermediates in the biosynthesis
CC       of flavonols, anthocyanidins, catechins and proanthocyanidins in
CC       plants.
CC   -!- CATALYTIC ACTIVITY: A flavanone + 2-oxoglutarate + O(2) = a
CC       dihydroflavonol + succinate + CO(2).
CC   -!- COFACTOR: Iron.
CC   -!- COFACTOR: Ascorbate.
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid
CC       biosynthesis.
CC   -!- SIMILARITY: Belongs to the iron/ascorbate-dependent oxidoreductase
CC       family.
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DR   EMBL; X72593; CAA51191.1; -; mRNA.
DR   PIR; S38336; S32147.
DR   HSSP; Q96323; 1GP4.
DR   BRENDA; 1.14.11.9; 275959.
DR   GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0031418; F:L-ascorbic acid binding; IEA:UniProtKB-KW.
DR   GO; GO:0045486; F:naringenin 3-dioxygenase activity; IEA:EC.
DR   GO; GO:0016702; F:oxidoreductase activity, acting on single d...; IEA:UniProtKB-KW.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR005123; Oxoglutarate/Fe-dep_Oase.
DR   Pfam; PF03171; 2OG-FeII_Oxy; 1.
PE   2: Evidence at transcript level;
KW   Dioxygenase; Flavonoid biosynthesis; Iron; Metal-binding;
KW   Oxidoreductase; Vitamin C.
FT   CHAIN         1    356       Naringenin,2-oxoglutarate 3-dioxygenase.
FT                                /FTId=PRO_0000067284.
FT   METAL        73     73       Iron (Potential).
FT   METAL       215    215       Iron (Potential).
FT   METAL       217    217       Iron (Potential).
FT   METAL       273    273       Iron (Potential).
SQ   SEQUENCE   356 AA;  40210 MW;  BE622A4A8C39BA0B CRC64;
     MAAPISLKWE EHSLHENKFV RDEDERPKVP YNTFSNEIPV ISLAGIDGCR RAEICDEIVK
     ACEDWGIFQV VDHGVDTKLL SDMTGLARDF FHLPTQEKLR FDMTGGKKGG FIVSSHLQGE
     AVQDWREIVT YFSYPIKARD YSRWPDKPNE WRAVTEEYSK VLMGLACKLL EVLSEAMGLE
     KEALTKACVD MDQKVVVNYY PKCPQPDLTL GLKRHTDPGT ITLLLQDQVG GLQATRDGGE
     SWITVKPVEG AFVVNLGDHG HYLSNGRFKN ADHQAVVNSS TSRLSIATFQ NPAPEAIVYP
     LKINEGEKSI MEEPMTFMEM YKKKMSTDLE LARLKKLAKD KQQDLEVVKP IQNIFA
//