Reviewed, UniProtKB/Swiss-Prot Q05963 (FL3H_CALCH)

Names and origin

Protein names

Recommended name:
    Naringenin,2-oxoglutarate 3-dioxygenase
    EC=1.14.11.9
Alternative name(s):
    Flavanone-3-hydroxylase
      Short name=F3H
    FHT

Gene names

Name:

FHT

Organism

Callistephus chinensis (China aster)

Taxonomic identifier

13379 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › campanulids › Asterales › Asteraceae › Asteroideae › Astereae › Callistephus

Protein attributes

Sequence length	356 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the 3-beta-hydroxylation of 2S-flavanones to 2R,3R-dihydroflavonols which are intermediates in the biosynthesis of flavonols, anthocyanidins, catechins and proanthocyanidins in plants.
Catalytic activity	A flavanone + 2-oxoglutarate + O2 = a dihydroflavonol + succinate + CO2.
Cofactor	Iron. Ascorbate.
Pathway	Secondary metabolite biosynthesis; flavonoid biosynthesis.
Sequence similarities	Belongs to the iron/ascorbate-dependent oxidoreductase family. Contains 1 Fe2OG dioxygenase domain.

Ontologies

Keywords
Biological process	Flavonoid biosynthesis
Ligand	Iron Metal-binding Vitamin C
Molecular function	Dioxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	flavonoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	L-ascorbic acid binding Inferred from electronic annotation. Source: UniProtKB-KW iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW naringenin 3-dioxygenase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 356	356	Naringenin,2-oxoglutarate 3-dioxygenase		PRO_0000067284
Regions
	Domain	188 – 292	105	Fe2OG dioxygenase
Sites
	Metal binding	73	1	Iron Potential
	Metal binding	215	1	Iron Potential
	Metal binding	217	1	Iron Potential
	Metal binding	273	1	Iron Potential

Sequences

	Sequence		Length	Mass (Da)
	Q05963-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: BE622A4A8C39BA0B	FASTA	356	40,210
	10 20 30 40 50 60 MAAPISLKWE EHSLHENKFV RDEDERPKVP YNTFSNEIPV ISLAGIDGCR RAEICDEIVK 70 80 90 100 110 120 ACEDWGIFQV VDHGVDTKLL SDMTGLARDF FHLPTQEKLR FDMTGGKKGG FIVSSHLQGE 130 140 150 160 170 180 AVQDWREIVT YFSYPIKARD YSRWPDKPNE WRAVTEEYSK VLMGLACKLL EVLSEAMGLE 190 200 210 220 230 240 KEALTKACVD MDQKVVVNYY PKCPQPDLTL GLKRHTDPGT ITLLLQDQVG GLQATRDGGE 250 260 270 280 290 300 SWITVKPVEG AFVVNLGDHG HYLSNGRFKN ADHQAVVNSS TSRLSIATFQ NPAPEAIVYP 310 320 330 340 350 LKINEGEKSI MEEPMTFMEM YKKKMSTDLE LARLKKLAKD KQQDLEVVKP IQNIFA

References

[1]

"Molecular characterization of flavanone 3 beta-hydroxylases. Consensus sequence, comparison with related enzymes and the role of conserved histidine residues." Britsch L., Dedio J., Saedler H., Forkmann G. Eur. J. Biochem. 217:745-754(1993) [PubMed: 8223617] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Flower bud.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X72593 mRNA. Translation: CAA51191.1.
PIR	S32147. S38336.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.14.11.9. 275959.
Family and domain databases
InterPro	IPR005123. Oxoglutarate/Fe-dep_oxygenase. [Graphical view]
Pfam	PF03171. 2OG-FeII_Oxy. 1 hit. [Graphical view]
PROSITE	PS51471. FE2OG_OXY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FL3H_CALCH

Accession

Primary (citable) accession number: Q05963

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	January 19, 2010
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)