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Reviewed, UniProtKB/Swiss-Prot Q05963 (FL3H_CALCH)

Last modified January 19, 2010. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Naringenin,2-oxoglutarate 3-dioxygenase
    EC=1.14.11.9
Alternative name(s):
    Flavanone-3-hydroxylase
      Short name=F3H
    FHT
Gene names
Name: FHT
OrganismCallistephus chinensis (China aster)
Taxonomic identifier13379 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonscore eudicotyledonsasteridscampanulidsAsteralesAsteraceaeAsteroideaeAstereaeCallistephus

Protein attributes

Sequence length356 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level.

General annotation (Comments)

Function

Catalyzes the 3-beta-hydroxylation of 2S-flavanones to 2R,3R-dihydroflavonols which are intermediates in the biosynthesis of flavonols, anthocyanidins, catechins and proanthocyanidins in plants.

Catalytic activity

A flavanone + 2-oxoglutarate + O2 = a dihydroflavonol + succinate + CO2.

Cofactor

Iron.

Ascorbate.

Pathway

Secondary metabolite biosynthesis; flavonoid biosynthesis.

Sequence similarities

Belongs to the iron/ascorbate-dependent oxidoreductase family.

Contains 1 Fe2OG dioxygenase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 356356Naringenin,2-oxoglutarate 3-dioxygenase
PRO_0000067284

Regions

Domain188 – 292105Fe2OG dioxygenase

Sites

Metal binding731Iron Potential
Metal binding2151Iron Potential
Metal binding2171Iron Potential
Metal binding2731Iron Potential

Sequences

Sequence LengthMass (Da)Tools
Q05963-1 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: BE622A4A8C39BA0B

FASTA35640,210
        10         20         30         40         50         60 
MAAPISLKWE EHSLHENKFV RDEDERPKVP YNTFSNEIPV ISLAGIDGCR RAEICDEIVK 

        70         80         90        100        110        120 
ACEDWGIFQV VDHGVDTKLL SDMTGLARDF FHLPTQEKLR FDMTGGKKGG FIVSSHLQGE 

       130        140        150        160        170        180 
AVQDWREIVT YFSYPIKARD YSRWPDKPNE WRAVTEEYSK VLMGLACKLL EVLSEAMGLE 

       190        200        210        220        230        240 
KEALTKACVD MDQKVVVNYY PKCPQPDLTL GLKRHTDPGT ITLLLQDQVG GLQATRDGGE 

       250        260        270        280        290        300 
SWITVKPVEG AFVVNLGDHG HYLSNGRFKN ADHQAVVNSS TSRLSIATFQ NPAPEAIVYP 

       310        320        330        340        350 
LKINEGEKSI MEEPMTFMEM YKKKMSTDLE LARLKKLAKD KQQDLEVVKP IQNIFA 

« Hide

References

[1]"Molecular characterization of flavanone 3 beta-hydroxylases. Consensus sequence, comparison with related enzymes and the role of conserved histidine residues."
Britsch L., Dedio J., Saedler H., Forkmann G.
Eur. J. Biochem. 217:745-754(1993) [PubMed: 8223617] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Flower bud.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X72593 mRNA. Translation: CAA51191.1.
PIRS32147. S38336.

3D structure databases

ModBaseSearch...

Enzyme and pathway databases

BRENDA1.14.11.9. 275959.

Family and domain databases

InterProIPR005123. Oxoglutarate/Fe-dep_oxygenase.
[Graphical view]
PfamPF03171. 2OG-FeII_Oxy. 1 hit.
[Graphical view]
PROSITEPS51471. FE2OG_OXY. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameFL3H_CALCH
AccessionPrimary (citable) accession number: Q05963
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: January 19, 2010
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents